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P35550

- FBRL_MOUSE

UniProt

P35550 - FBRL_MOUSE

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Protein

rRNA 2'-O-methyltransferase fibrillarin

Gene

Fbl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N(5)-methyl-L-glutamine-[histone].

GO - Molecular functioni

  1. histone-glutamine methyltransferase activity Source: UniProtKB
  2. poly(A) RNA binding Source: Ensembl
  3. snoRNA binding Source: MGI

GO - Biological processi

  1. histone glutamine methylation Source: UniProtKB
  2. osteoblast differentiation Source: Ensembl
  3. rRNA processing Source: MGI
  4. snoRNA metabolic process Source: MGI
  5. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Ribonucleoprotein, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA 2'-O-methyltransferase fibrillarin (EC:2.1.1.-)
Alternative name(s):
Histone-glutamine methyltransferase
Nucleolar protein 1
Gene namesi
Name:Fbl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:95486. Fbl.

Subcellular locationi

Nucleusnucleolus
Note: Fibrillar region of the nucleolus.

GO - Cellular componenti

  1. Cajal body Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. granular component Source: MGI
  4. membrane Source: Ensembl
  5. nucleolus Source: MGI
  6. ribonucleoprotein complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327rRNA 2'-O-methyltransferase fibrillarinPRO_0000148508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Asymmetric dimethylarginineBy similarity
Modified residuei15 – 151Asymmetric dimethylarginineBy similarity
Modified residuei21 – 211Asymmetric dimethylarginineBy similarity
Modified residuei24 – 241Asymmetric dimethylarginineBy similarity
Modified residuei28 – 281Asymmetric dimethylarginineBy similarity
Modified residuei31 – 311Asymmetric dimethylarginineBy similarity
Modified residuei130 – 1301PhosphoserineBy similarity

Post-translational modificationi

By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiP35550.
PaxDbiP35550.
PRIDEiP35550.

PTM databases

PhosphoSiteiP35550.

Expressioni

Gene expression databases

BgeeiP35550.
CleanExiMM_FBL.
GenevestigatoriP35550.

Interactioni

Subunit structurei

Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5, NOLC1 and UTP20. Interacts with DDX5 and C1QBP (By similarity). Interacts with NOL11 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199604. 1 interaction.
IntActiP35550. 6 interactions.
MINTiMINT-1854224.

Structurei

3D structure databases

ProteinModelPortaliP35550.
SMRiP35550. Positions 93-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni178 – 1792S-adenosyl-L-methionine bindingBy similarity
Regioni197 – 1982S-adenosyl-L-methionine bindingBy similarity
Regioni222 – 2232S-adenosyl-L-methionine bindingBy similarity
Regioni242 – 2454S-adenosyl-L-methionine bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 8582DMA/Gly-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1889.
GeneTreeiENSGT00550000074792.
HOGENOMiHOG000106741.
HOVERGENiHBG002472.
InParanoidiP35550.
KOiK14563.
OMAiWNPNKSK.
OrthoDBiEOG7KWSJP.
PhylomeDBiP35550.
TreeFamiTF300639.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35550 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPGFSPRGG GFGGRGGFGD RGGRGGGRGG RGGFGGGRGG FGGGGRGRGG
60 70 80 90 100
GGGGFRGRGG GGGRGGGFQS GGNRGRGGGR GGKRGNQSGK NVMVEPHRHE
110 120 130 140 150
GVFICRGKED ALVTKNLVPG ESVYGEKRVS ISEGDDKIEY RAWNPFRSKL
160 170 180 190 200
AAAILGGVDQ IHIKPGAKVL YLGAASGTTV SHVSDIVGPD GLVYAVEFSH
210 220 230 240 250
RSGRDLINLA KKRTNIIPVI EDARHPHKYR MLIAMVDVIF ADVAQPDQTR
260 270 280 290 300
IVALNAHTFL RNGGHFVISI KANCIDSTAS AEAVFASEVK KMQQENMKPQ
310 320
EQLTLEPYER DHAVVVGVYR PPPKVKN
Length:327
Mass (Da):34,307
Last modified:August 30, 2005 - v2
Checksum:i0E22FE7C758089C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61S → R in CAA80307. (PubMed:8218401)Curated
Sequence conflicti63 – 631G → V in CAA80307. (PubMed:8218401)Curated
Sequence conflicti113 – 1131V → F in CAA80307. (PubMed:8218401)Curated
Sequence conflicti137 – 1371K → T in CAA80307. (PubMed:8218401)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22593 mRNA. Translation: CAA80307.1.
BC003813 mRNA. Translation: AAH03813.1.
BC092274 mRNA. Translation: AAH92274.1.
CCDSiCCDS21036.1.
PIRiS38342.
RefSeqiNP_032017.2. NM_007991.3.
XP_006543829.1. XM_006543766.1.
UniGeneiMm.4595.

Genome annotation databases

EnsembliENSMUST00000042405; ENSMUSP00000037613; ENSMUSG00000046865.
GeneIDi102643269.
14113.
KEGGimmu:102643269.
mmu:14113.
UCSCiuc009fxy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22593 mRNA. Translation: CAA80307.1 .
BC003813 mRNA. Translation: AAH03813.1 .
BC092274 mRNA. Translation: AAH92274.1 .
CCDSi CCDS21036.1.
PIRi S38342.
RefSeqi NP_032017.2. NM_007991.3.
XP_006543829.1. XM_006543766.1.
UniGenei Mm.4595.

3D structure databases

ProteinModelPortali P35550.
SMRi P35550. Positions 93-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199604. 1 interaction.
IntActi P35550. 6 interactions.
MINTi MINT-1854224.

PTM databases

PhosphoSitei P35550.

Proteomic databases

MaxQBi P35550.
PaxDbi P35550.
PRIDEi P35550.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000042405 ; ENSMUSP00000037613 ; ENSMUSG00000046865 .
GeneIDi 102643269.
14113.
KEGGi mmu:102643269.
mmu:14113.
UCSCi uc009fxy.1. mouse.

Organism-specific databases

CTDi 2091.
MGIi MGI:95486. Fbl.

Phylogenomic databases

eggNOGi COG1889.
GeneTreei ENSGT00550000074792.
HOGENOMi HOG000106741.
HOVERGENi HBG002472.
InParanoidi P35550.
KOi K14563.
OMAi WNPNKSK.
OrthoDBi EOG7KWSJP.
PhylomeDBi P35550.
TreeFami TF300639.

Miscellaneous databases

ChiTaRSi FBL. mouse.
NextBioi 285166.
PROi P35550.
SOURCEi Search...

Gene expression databases

Bgeei P35550.
CleanExi MM_FBL.
Genevestigatori P35550.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_00351. RNA_methyltransf_FlpA.
InterProi IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01269. Fibrillarin. 1 hit.
[Graphical view ]
PIRSFi PIRSF006540. Nop17p. 1 hit.
PRINTSi PR00052. FIBRILLARIN.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00566. FIBRILLARIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequence analysis of U3 snoRNA-associated mouse fibrillarin."
    Turley S.J., Tan E.M., Pollard K.M.
    Biochim. Biophys. Acta 1216:119-122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Macrophage.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver and Mammary tumor.

Entry informationi

Entry nameiFBRL_MOUSE
AccessioniPrimary (citable) accession number: P35550
Secondary accession number(s): Q99L58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 30, 2005
Last modified: October 29, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3