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P35550

- FBRL_MOUSE

UniProt

P35550 - FBRL_MOUSE

Protein

rRNA 2'-O-methyltransferase fibrillarin

Gene

Fbl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N(5)-methyl-L-glutamine-[histone].

    GO - Molecular functioni

    1. histone-glutamine methyltransferase activity Source: UniProtKB
    2. snoRNA binding Source: MGI

    GO - Biological processi

    1. histone glutamine methylation Source: UniProtKB
    2. rRNA processing Source: MGI
    3. snoRNA metabolic process Source: MGI
    4. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Ribonucleoprotein, Transferase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    rRNA 2'-O-methyltransferase fibrillarin (EC:2.1.1.-)
    Alternative name(s):
    Histone-glutamine methyltransferase
    Nucleolar protein 1
    Gene namesi
    Name:Fbl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:95486. Fbl.

    Subcellular locationi

    Nucleusnucleolus
    Note: Fibrillar region of the nucleolus.

    GO - Cellular componenti

    1. Cajal body Source: MGI
    2. granular component Source: MGI
    3. nucleolus Source: MGI
    4. ribonucleoprotein complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327rRNA 2'-O-methyltransferase fibrillarinPRO_0000148508Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei8 – 81Asymmetric dimethylarginineBy similarity
    Modified residuei15 – 151Asymmetric dimethylarginineBy similarity
    Modified residuei21 – 211Asymmetric dimethylarginineBy similarity
    Modified residuei24 – 241Asymmetric dimethylarginineBy similarity
    Modified residuei28 – 281Asymmetric dimethylarginineBy similarity
    Modified residuei31 – 311Asymmetric dimethylarginineBy similarity
    Modified residuei130 – 1301PhosphoserineBy similarity

    Post-translational modificationi

    By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiP35550.
    PaxDbiP35550.
    PRIDEiP35550.

    PTM databases

    PhosphoSiteiP35550.

    Expressioni

    Gene expression databases

    BgeeiP35550.
    CleanExiMM_FBL.
    GenevestigatoriP35550.

    Interactioni

    Subunit structurei

    Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5, NOLC1 and UTP20. Interacts with DDX5 and C1QBP By similarity. Interacts with NOL11 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi199604. 1 interaction.
    IntActiP35550. 6 interactions.
    MINTiMINT-1854224.

    Structurei

    3D structure databases

    ProteinModelPortaliP35550.
    SMRiP35550. Positions 93-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni178 – 1792S-adenosyl-L-methionine bindingBy similarity
    Regioni197 – 1982S-adenosyl-L-methionine bindingBy similarity
    Regioni222 – 2232S-adenosyl-L-methionine bindingBy similarity
    Regioni242 – 2454S-adenosyl-L-methionine bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 8582DMA/Gly-richAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1889.
    GeneTreeiENSGT00550000074792.
    HOGENOMiHOG000106741.
    HOVERGENiHBG002472.
    InParanoidiP35550.
    KOiK14563.
    OMAiWNPNKSK.
    OrthoDBiEOG7KWSJP.
    PhylomeDBiP35550.
    TreeFamiTF300639.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_00351. RNA_methyltransf_FlpA.
    InterProiIPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01269. Fibrillarin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006540. Nop17p. 1 hit.
    PRINTSiPR00052. FIBRILLARIN.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00566. FIBRILLARIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35550-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPGFSPRGG GFGGRGGFGD RGGRGGGRGG RGGFGGGRGG FGGGGRGRGG    50
    GGGGFRGRGG GGGRGGGFQS GGNRGRGGGR GGKRGNQSGK NVMVEPHRHE 100
    GVFICRGKED ALVTKNLVPG ESVYGEKRVS ISEGDDKIEY RAWNPFRSKL 150
    AAAILGGVDQ IHIKPGAKVL YLGAASGTTV SHVSDIVGPD GLVYAVEFSH 200
    RSGRDLINLA KKRTNIIPVI EDARHPHKYR MLIAMVDVIF ADVAQPDQTR 250
    IVALNAHTFL RNGGHFVISI KANCIDSTAS AEAVFASEVK KMQQENMKPQ 300
    EQLTLEPYER DHAVVVGVYR PPPKVKN 327
    Length:327
    Mass (Da):34,307
    Last modified:August 30, 2005 - v2
    Checksum:i0E22FE7C758089C3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61S → R in CAA80307. (PubMed:8218401)Curated
    Sequence conflicti63 – 631G → V in CAA80307. (PubMed:8218401)Curated
    Sequence conflicti113 – 1131V → F in CAA80307. (PubMed:8218401)Curated
    Sequence conflicti137 – 1371K → T in CAA80307. (PubMed:8218401)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22593 mRNA. Translation: CAA80307.1.
    BC003813 mRNA. Translation: AAH03813.1.
    BC092274 mRNA. Translation: AAH92274.1.
    CCDSiCCDS21036.1.
    PIRiS38342.
    RefSeqiNP_032017.2. NM_007991.3.
    XP_006543829.1. XM_006543766.1.
    UniGeneiMm.4595.

    Genome annotation databases

    EnsembliENSMUST00000042405; ENSMUSP00000037613; ENSMUSG00000046865.
    GeneIDi102643269.
    14113.
    KEGGimmu:102643269.
    mmu:14113.
    UCSCiuc009fxy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22593 mRNA. Translation: CAA80307.1 .
    BC003813 mRNA. Translation: AAH03813.1 .
    BC092274 mRNA. Translation: AAH92274.1 .
    CCDSi CCDS21036.1.
    PIRi S38342.
    RefSeqi NP_032017.2. NM_007991.3.
    XP_006543829.1. XM_006543766.1.
    UniGenei Mm.4595.

    3D structure databases

    ProteinModelPortali P35550.
    SMRi P35550. Positions 93-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199604. 1 interaction.
    IntActi P35550. 6 interactions.
    MINTi MINT-1854224.

    PTM databases

    PhosphoSitei P35550.

    Proteomic databases

    MaxQBi P35550.
    PaxDbi P35550.
    PRIDEi P35550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042405 ; ENSMUSP00000037613 ; ENSMUSG00000046865 .
    GeneIDi 102643269.
    14113.
    KEGGi mmu:102643269.
    mmu:14113.
    UCSCi uc009fxy.1. mouse.

    Organism-specific databases

    CTDi 2091.
    MGIi MGI:95486. Fbl.

    Phylogenomic databases

    eggNOGi COG1889.
    GeneTreei ENSGT00550000074792.
    HOGENOMi HOG000106741.
    HOVERGENi HBG002472.
    InParanoidi P35550.
    KOi K14563.
    OMAi WNPNKSK.
    OrthoDBi EOG7KWSJP.
    PhylomeDBi P35550.
    TreeFami TF300639.

    Miscellaneous databases

    ChiTaRSi FBL. mouse.
    NextBioi 285166.
    PROi P35550.
    SOURCEi Search...

    Gene expression databases

    Bgeei P35550.
    CleanExi MM_FBL.
    Genevestigatori P35550.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_00351. RNA_methyltransf_FlpA.
    InterProi IPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01269. Fibrillarin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006540. Nop17p. 1 hit.
    PRINTSi PR00052. FIBRILLARIN.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00566. FIBRILLARIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequence analysis of U3 snoRNA-associated mouse fibrillarin."
      Turley S.J., Tan E.M., Pollard K.M.
      Biochim. Biophys. Acta 1216:119-122(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Macrophage.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver and Mammary tumor.

    Entry informationi

    Entry nameiFBRL_MOUSE
    AccessioniPrimary (citable) accession number: P35550
    Secondary accession number(s): Q99L58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3