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Protein

rRNA 2'-O-methyltransferase fibrillarin

Gene

Fbl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N5-methyl-L-glutamine-[histone].

GO - Molecular functioni

GO - Biological processi

  • box C/D snoRNA 3'-end processing Source: GO_Central
  • histone glutamine methylation Source: UniProtKB
  • rRNA methylation Source: GO_Central
  • snoRNA localization Source: MGI
  • snoRNA metabolic process Source: MGI

Keywordsi

Molecular functionMethyltransferase, Ribonucleoprotein, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA 2'-O-methyltransferase fibrillarin (EC:2.1.1.-)
Alternative name(s):
Histone-glutamine methyltransferase
Nucleolar protein 1
Gene namesi
Name:Fbl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95486 Fbl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485081 – 327rRNA 2'-O-methyltransferase fibrillarinAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8Asymmetric dimethylarginineBy similarity1
Modified residuei15Asymmetric dimethylarginineBy similarity1
Modified residuei21Asymmetric dimethylarginineBy similarity1
Modified residuei24Asymmetric dimethylarginineBy similarity1
Modified residuei28Asymmetric dimethylarginineBy similarity1
Modified residuei31Asymmetric dimethylarginineBy similarity1
Cross-linki90Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei122PhosphoserineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei132PhosphoserineBy similarity1
Cross-linki137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP35550
MaxQBiP35550
PaxDbiP35550
PeptideAtlasiP35550
PRIDEiP35550

PTM databases

iPTMnetiP35550
PhosphoSitePlusiP35550

Expressioni

Gene expression databases

BgeeiENSMUSG00000046865
CleanExiMM_FBL
ExpressionAtlasiP35550 baseline and differential
GenevisibleiP35550 MM

Interactioni

Subunit structurei

Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5, NOLC1 and UTP20. Interacts with DDX5 and C1QBP. Interacts with NOL11. Interacts with PHI1D1. Interacts with RRP1B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi19960428 interactors.
CORUMiP35550
IntActiP35550 31 interactors.
MINTiP35550
STRINGi10090.ENSMUSP00000037613

Structurei

3D structure databases

ProteinModelPortaliP35550
SMRiP35550
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni178 – 179S-adenosyl-L-methionine bindingBy similarity2
Regioni197 – 198S-adenosyl-L-methionine bindingBy similarity2
Regioni222 – 223S-adenosyl-L-methionine bindingBy similarity2
Regioni242 – 245S-adenosyl-L-methionine bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 85DMA/Gly-richAdd BLAST82

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1596 Eukaryota
COG1889 LUCA
GeneTreeiENSGT00550000074792
HOGENOMiHOG000106741
HOVERGENiHBG002472
InParanoidiP35550
KOiK14563
OMAiQPNQAEI
OrthoDBiEOG091G0GV0
PhylomeDBiP35550
TreeFamiTF300639

Family and domain databases

HAMAPiMF_00351 RNA_methyltransf_FlpA, 1 hit
InterProiView protein in InterPro
IPR000692 Fibrillarin
IPR020813 Fibrillarin_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01269 Fibrillarin, 1 hit
PIRSFiPIRSF006540 Nop17p, 1 hit
PRINTSiPR00052 FIBRILLARIN
SMARTiView protein in SMART
SM01206 Fibrillarin, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS00566 FIBRILLARIN, 1 hit

Sequencei

Sequence statusi: Complete.

P35550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPGFSPRGG GFGGRGGFGD RGGRGGGRGG RGGFGGGRGG FGGGGRGRGG
60 70 80 90 100
GGGGFRGRGG GGGRGGGFQS GGNRGRGGGR GGKRGNQSGK NVMVEPHRHE
110 120 130 140 150
GVFICRGKED ALVTKNLVPG ESVYGEKRVS ISEGDDKIEY RAWNPFRSKL
160 170 180 190 200
AAAILGGVDQ IHIKPGAKVL YLGAASGTTV SHVSDIVGPD GLVYAVEFSH
210 220 230 240 250
RSGRDLINLA KKRTNIIPVI EDARHPHKYR MLIAMVDVIF ADVAQPDQTR
260 270 280 290 300
IVALNAHTFL RNGGHFVISI KANCIDSTAS AEAVFASEVK KMQQENMKPQ
310 320
EQLTLEPYER DHAVVVGVYR PPPKVKN
Length:327
Mass (Da):34,307
Last modified:August 30, 2005 - v2
Checksum:i0E22FE7C758089C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6S → R in CAA80307 (PubMed:8218401).Curated1
Sequence conflicti63G → V in CAA80307 (PubMed:8218401).Curated1
Sequence conflicti113V → F in CAA80307 (PubMed:8218401).Curated1
Sequence conflicti137K → T in CAA80307 (PubMed:8218401).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22593 mRNA Translation: CAA80307.1
BC003813 mRNA Translation: AAH03813.1
BC092274 mRNA Translation: AAH92274.1
CCDSiCCDS21036.1
PIRiS38342
RefSeqiNP_032017.2, NM_007991.3
UniGeneiMm.4595

Genome annotation databases

EnsembliENSMUST00000042405; ENSMUSP00000037613; ENSMUSG00000046865
GeneIDi14113
KEGGimmu:14113
UCSCiuc009fxy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiFBRL_MOUSE
AccessioniPrimary (citable) accession number: P35550
Secondary accession number(s): Q99L58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 30, 2005
Last modified: April 25, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome