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Protein

Chloride intracellular channel protein 5

Gene

CLIC5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for normal hearing. It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti. Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (By similarity).By similarity

GO - Molecular functioni

  • chloride channel activity Source: UniProtKB
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

  • auditory receptor cell stereocilium organization Source: GO_Central
  • chloride transport Source: UniProtKB
  • sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Hearing, Ion transport, Transport

Keywords - Ligandi

Chloride

Protein family/group databases

TCDBi1.A.12.1.1. the intracellular chloride channel (clic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 5
Alternative name(s):
Chlorine channel protein p64
Gene namesi
Name:CLIC5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei219 – 23921Helical; Note=After insertion into the membraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB
  • Golgi apparatus Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: UniProtKB
  • microtubule organizing center Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Chloride intracellular channel protein 5PRO_0000144213Add
BLAST

Post-translational modificationi

Phosphorylated.Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35526.
PRIDEiP35526.

Expressioni

Tissue specificityi

Expressed in most tissues. Higher levels found in kidney, heart, skeletal muscle, T84 and PANC-1 cells.

Interactioni

Subunit structurei

Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1.By similarity

Protein-protein interaction databases

BioGridi159015. 1 interaction.
STRINGi9913.ENSBTAP00000013537.

Structurei

3D structure databases

ProteinModelPortaliP35526.
SMRiP35526. Positions 191-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati118 – 12581
Repeati126 – 13382
Repeati134 – 14183
Repeati142 – 14984
Domaini286 – 427142GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 149324 X 8 AA tandem repeats of [AGQ]-[SP]-D-[PS]-E-E-P-QAdd
BLAST

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
HOGENOMiHOG000065740.
HOVERGENiHBG050995.
InParanoidiP35526.
KOiK05025.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030264. CLIC-5.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF152. PTHR11260:SF152. 2 hits.
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDENYSTTI YNRVQTERVY EDSDPAENGG PLYDEVHEDV RREDNLYVNE
60 70 80 90 100
LENQEYDSVA VYPVGRQGRT SASLQPETGE YVLPDEPYSK AQDPHPGEPT
110 120 130 140 150
ADEDISLEEL LSPTKDHQSD SEEPQASDPE EPQASDPEEP QGPDPEEPQE
160 170 180 190 200
NGNEMEADLP SPSSFTIQNS RAFSTREISP TSYSADDVSE GNESASASPE
210 220 230 240 250
INLFVKAGID GESIGNCPFS QRLFMILWLK GVVFNVTTVD LKRKPADLHN
260 270 280 290 300
LAPGTHPPFL TFNGDVKTDV NKIEEFLEET LTPEKYPRLA AKHRESNTAG
310 320 330 340 350
IDIFVKFSAY IKNTKQQSNA ALERGLTKAL KKLDDYLNTP LPEEIDADTR
360 370 380 390 400
GDDEKGSRRK FLDGDELTLA DCNLLPKLHV VKIVAKKYRN YDFPAEMTGL
410 420 430
WRYLKNAYAR DEFTNTCAAD SEIELAYADV AKRLSRS
Length:437
Mass (Da):48,959
Last modified:June 1, 1994 - v1
Checksum:iFE5522856BBE799F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16547 mRNA. Translation: AAA02561.1.
AF109199 mRNA. Translation: AAD26139.1.
PIRiA47104.
RefSeqiNP_776701.1. NM_174276.3.
UniGeneiBt.194.

Genome annotation databases

GeneIDi281696.
KEGGibta:281696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16547 mRNA. Translation: AAA02561.1.
AF109199 mRNA. Translation: AAD26139.1.
PIRiA47104.
RefSeqiNP_776701.1. NM_174276.3.
UniGeneiBt.194.

3D structure databases

ProteinModelPortaliP35526.
SMRiP35526. Positions 191-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159015. 1 interaction.
STRINGi9913.ENSBTAP00000013537.

Protein family/group databases

TCDBi1.A.12.1.1. the intracellular chloride channel (clic) family.

Proteomic databases

PaxDbiP35526.
PRIDEiP35526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281696.
KEGGibta:281696.

Organism-specific databases

CTDi53405.

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
HOGENOMiHOG000065740.
HOVERGENiHBG050995.
InParanoidiP35526.
KOiK05025.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030264. CLIC-5.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF152. PTHR11260:SF152. 2 hits.
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of p64, a chloride channel protein from kidney microsomes."
    Landry D.W., Sullivan S., Nicolaides M., Redhead C., Edelman A., Field M., Al-Awqati Q., Edwards J.F.
    J. Biol. Chem. 268:14948-14955(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons."
    Chuang J.Z., Milner T.A., Zhu M., Sung C.H.
    J. Neurosci. 19:2919-2928(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.

Entry informationi

Entry nameiCLIC5_BOVIN
AccessioniPrimary (citable) accession number: P35526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.