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Protein

Chloride channel protein 2

Gene

Clcn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel (PubMed:1311421, PubMed:12163466). Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport (PubMed:1311421).2 Publications

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

GO - Molecular functioni

  • voltage-gated chloride channel activity Source: RGD

GO - Biological processi

  • cell differentiation involved in salivary gland development Source: RGD
  • chloride transport Source: RGD
  • lung development Source: RGD
  • regulation of ion transmembrane transport Source: UniProtKB-KW
  • retina development in camera-type eye Source: RGD

Keywordsi

Molecular functionChloride channel, Ion channel, Voltage-gated channel
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-RNO-2672351 Stimuli-sensing channels

Protein family/group databases

TCDBi2.A.49.2.6 the chloride carrier/channel (clc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 2
Short name:
ClC-2
Gene namesi
Name:Clcn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2361 Clcn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 93CytoplasmicBy similarityAdd BLAST93
Transmembranei94 – 127HelicalBy similarityAdd BLAST34
Transmembranei136 – 161HelicalBy similarityAdd BLAST26
Intramembranei170 – 177HelicalSequence analysis8
Transmembranei186 – 204HelicalBy similarityAdd BLAST19
Transmembranei211 – 229HelicalBy similarityAdd BLAST19
Intramembranei245 – 257HelicalBy similarityAdd BLAST13
Intramembranei261 – 269HelicalBy similarity9
Transmembranei281 – 301HelicalBy similarityAdd BLAST21
Transmembranei327 – 355HelicalBy similarityAdd BLAST29
Transmembranei364 – 383HelicalBy similarityAdd BLAST20
Transmembranei435 – 455HelicalBy similarityAdd BLAST21
Transmembranei463 – 486HelicalBy similarityAdd BLAST24
Intramembranei503 – 517HelicalBy similarityAdd BLAST15
Intramembranei518 – 519Note=Loop between two helicesBy similarity2
Intramembranei520 – 531HelicalBy similarityAdd BLAST12
Intramembranei532 – 536Note=Loop between two helicesBy similarity5
Transmembranei537 – 554HelicalBy similarityAdd BLAST18
Topological domaini555 – 907CytoplasmicBy similarityAdd BLAST353

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944361 – 907Chloride channel protein 2Add BLAST907

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphothreonineBy similarity1
Modified residuei767PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. Activated by dephosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35525
PRIDEiP35525

PTM databases

iPTMnetiP35525
PhosphoSitePlusiP35525

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSRNOG00000001742
GenevisibleiP35525 RN

Interactioni

Protein-protein interaction databases

CORUMiP35525
STRINGi10116.ENSRNOP00000066524

Structurei

3D structure databases

ProteinModelPortaliP35525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini590 – 648CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini799 – 859CBS 2PROSITE-ProRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi167 – 171Selectivity filter part_1By similarity5
Motifi209 – 213Selectivity filter part_2By similarity5
Motifi463 – 467Selectivity filter part_3By similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 9Poly-Ala8

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
GeneTreeiENSGT00760000119109
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiP35525
KOiK05011
OMAiWAMDYAI
OrthoDBiEOG091G01RJ
PhylomeDBiP35525
TreeFamiTF300522

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR002244 Cl-channel-2
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
PfamiView protein in Pfam
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01113 CLCHANNEL2
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits

Sequencei

Sequence statusi: Complete.

P35525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATAAAAT VAGEGMEPRA LQYEQTLMYG RYTQELGAFA KEEAARIRLG
60 70 80 90 100
GPEPWKGSPS ARATPELLEY GQSRCARCRI CSVRCHKFLV SRVGEDWIFL
110 120 130 140 150
VLLGLLMALV SWAMDYAIAV CLQAQQWMSR GLNTNILLQY LAWVTYPVVL
160 170 180 190 200
ITFSAGFTQI LAPQAVGSGI PEMKTILRGV VLKEYLTLKT FVAKVIGLTC
210 220 230 240 250
ALGSGMPLGK EGPFVHIASM CAALLSKFLS LFGGIYENES RNTEMLAAAC
260 270 280 290 300
AVGVGCCFAA PIGGVLFSIE VTSTFFAVRN YWRGFFAATF SAFIFRVLAV
310 320 330 340 350
WNRDEETITA LFKTRFRLDF PFDLQELPAF AVIGIASGFG GALFVYLNRK
360 370 380 390 400
IVQVMRKQKT INRFLMKKRL LFPALVTLLI STLTFPPGFG QFMAGQLSQK
410 420 430 440 450
ETLVTLFDNR TWVRQGLVED LGAPSTSQAW SPPRANVFLT LVIFILMKFW
460 470 480 490 500
MSALATTIPV PCGAFMPVFV IGAAFGRLVG ESMAAWFPDG IHTDSSTYRI
510 520 530 540 550
VPGGYAVVGA AALAGAVTHT VSTAVIVFEL TGQIAHILPV MIAVILANAV
560 570 580 590 600
AQSLQPSLYD SIIRIKKLPY LPELGWGRHQ QYRVRVEDIM VRDVPHVALS
610 620 630 640 650
CTFRDLRLAL HRTKGRMLAL VESPESMILL GSIERSQVVA LLGAQLSPAR
660 670 680 690 700
RRQHMQKLRK AQMSPPSDQE SPPSSETSIR FQVNTEDSGF PGAHGQTHKP
710 720 730 740 750
LKPALKRGPS NATSLQEGTT GNMESAGIAL RSLFCGSPPL ESTTSELEKS
760 770 780 790 800
ESCDKRKLKR VRISLASDSD LEGKMSPEEI LEWEEQQLDE PVNFSDCKID
810 820 830 840 850
PAPFQLVERT SLHKTHTIFS LLGVDHAYVT SIGRLIGIVT LKELRKAIEG
860 870 880 890 900
SVTAQGVKVR PPLASFRDSA TSSSDTETTE VHALWGPRSR HGLPREGTPS

DSDDKCQ
Length:907
Mass (Da):99,328
Last modified:June 1, 1994 - v1
Checksum:i6192EEB2EE0D6528
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64139 mRNA Translation: CAA45500.1
PIRiS23399
RefSeqiNP_058833.1, NM_017137.1
XP_006248624.1, XM_006248562.3
UniGeneiRn.11073

Genome annotation databases

EnsembliENSRNOT00000050927; ENSRNOP00000050925; ENSRNOG00000001742
ENSRNOT00000071707; ENSRNOP00000066524; ENSRNOG00000050854
GeneIDi108348101
29232
KEGGirno:108348101
rno:29232

Similar proteinsi

Entry informationi

Entry nameiCLCN2_RAT
AccessioniPrimary (citable) accession number: P35525
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health