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Protein

Chloride channel protein 2

Gene

Clcn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel (PubMed:1311421, PubMed:12163466). Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport (PubMed:1311421).2 Publications

GO - Molecular functioni

  • voltage-gated chloride channel activity Source: RGD

GO - Biological processi

  • chloride transport Source: RGD
  • lung development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi2.A.49.2.6. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 2
Short name:
ClC-2
Gene namesi
Name:Clcn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2361. Clcn2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9393CytoplasmicBy similarityAdd
BLAST
Transmembranei94 – 12734HelicalBy similarityAdd
BLAST
Transmembranei136 – 16126HelicalBy similarityAdd
BLAST
Intramembranei170 – 1778HelicalSequence analysis
Transmembranei186 – 20419HelicalBy similarityAdd
BLAST
Transmembranei211 – 22919HelicalBy similarityAdd
BLAST
Intramembranei245 – 25713HelicalBy similarityAdd
BLAST
Intramembranei261 – 2699HelicalBy similarity
Transmembranei281 – 30121HelicalBy similarityAdd
BLAST
Transmembranei327 – 35529HelicalBy similarityAdd
BLAST
Transmembranei364 – 38320HelicalBy similarityAdd
BLAST
Transmembranei435 – 45521HelicalBy similarityAdd
BLAST
Transmembranei463 – 48624HelicalBy similarityAdd
BLAST
Intramembranei503 – 51715HelicalBy similarityAdd
BLAST
Intramembranei518 – 5192Note=Loop between two helicesBy similarity
Intramembranei520 – 53112HelicalBy similarityAdd
BLAST
Intramembranei532 – 5365Note=Loop between two helicesBy similarity
Transmembranei537 – 55418HelicalBy similarityAdd
BLAST
Topological domaini555 – 907353CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • chloride channel complex Source: UniProtKB-KW
  • dendrite Source: RGD
  • perikaryon Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 907907Chloride channel protein 2PRO_0000094436Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261PhosphothreonineBy similarity
Modified residuei767 – 7671PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated. Activated by dephosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35525.
PRIDEiP35525.

PTM databases

iPTMnetiP35525.
PhosphoSiteiP35525.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSRNOG00000001742.
GenevisibleiP35525. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996661.
STRINGi10116.ENSRNOP00000066524.

Structurei

3D structure databases

ProteinModelPortaliP35525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini590 – 64859CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini799 – 85961CBS 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi167 – 1715Selectivity filter part_1By similarity
Motifi209 – 2135Selectivity filter part_2By similarity
Motifi463 – 4675Selectivity filter part_3By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 98Poly-Ala

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP35525.
KOiK05011.
OMAiESCEKRK.
OrthoDBiEOG091G01RJ.
PhylomeDBiP35525.
TreeFamiTF300522.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR002244. Cl-channel-2.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01113. CLCHANNEL2.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATAAAAT VAGEGMEPRA LQYEQTLMYG RYTQELGAFA KEEAARIRLG
60 70 80 90 100
GPEPWKGSPS ARATPELLEY GQSRCARCRI CSVRCHKFLV SRVGEDWIFL
110 120 130 140 150
VLLGLLMALV SWAMDYAIAV CLQAQQWMSR GLNTNILLQY LAWVTYPVVL
160 170 180 190 200
ITFSAGFTQI LAPQAVGSGI PEMKTILRGV VLKEYLTLKT FVAKVIGLTC
210 220 230 240 250
ALGSGMPLGK EGPFVHIASM CAALLSKFLS LFGGIYENES RNTEMLAAAC
260 270 280 290 300
AVGVGCCFAA PIGGVLFSIE VTSTFFAVRN YWRGFFAATF SAFIFRVLAV
310 320 330 340 350
WNRDEETITA LFKTRFRLDF PFDLQELPAF AVIGIASGFG GALFVYLNRK
360 370 380 390 400
IVQVMRKQKT INRFLMKKRL LFPALVTLLI STLTFPPGFG QFMAGQLSQK
410 420 430 440 450
ETLVTLFDNR TWVRQGLVED LGAPSTSQAW SPPRANVFLT LVIFILMKFW
460 470 480 490 500
MSALATTIPV PCGAFMPVFV IGAAFGRLVG ESMAAWFPDG IHTDSSTYRI
510 520 530 540 550
VPGGYAVVGA AALAGAVTHT VSTAVIVFEL TGQIAHILPV MIAVILANAV
560 570 580 590 600
AQSLQPSLYD SIIRIKKLPY LPELGWGRHQ QYRVRVEDIM VRDVPHVALS
610 620 630 640 650
CTFRDLRLAL HRTKGRMLAL VESPESMILL GSIERSQVVA LLGAQLSPAR
660 670 680 690 700
RRQHMQKLRK AQMSPPSDQE SPPSSETSIR FQVNTEDSGF PGAHGQTHKP
710 720 730 740 750
LKPALKRGPS NATSLQEGTT GNMESAGIAL RSLFCGSPPL ESTTSELEKS
760 770 780 790 800
ESCDKRKLKR VRISLASDSD LEGKMSPEEI LEWEEQQLDE PVNFSDCKID
810 820 830 840 850
PAPFQLVERT SLHKTHTIFS LLGVDHAYVT SIGRLIGIVT LKELRKAIEG
860 870 880 890 900
SVTAQGVKVR PPLASFRDSA TSSSDTETTE VHALWGPRSR HGLPREGTPS

DSDDKCQ
Length:907
Mass (Da):99,328
Last modified:June 1, 1994 - v1
Checksum:i6192EEB2EE0D6528
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64139 mRNA. Translation: CAA45500.1.
PIRiS23399.
RefSeqiNP_058833.1. NM_017137.1.
XP_003751129.1. XM_003751081.3.
XP_006248624.1. XM_006248562.2.
UniGeneiRn.11073.

Genome annotation databases

EnsembliENSRNOT00000050927; ENSRNOP00000050925; ENSRNOG00000001742.
ENSRNOT00000071707; ENSRNOP00000066524; ENSRNOG00000050854.
GeneIDi100912455.
29232.
KEGGirno:100912455.
rno:29232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64139 mRNA. Translation: CAA45500.1.
PIRiS23399.
RefSeqiNP_058833.1. NM_017137.1.
XP_003751129.1. XM_003751081.3.
XP_006248624.1. XM_006248562.2.
UniGeneiRn.11073.

3D structure databases

ProteinModelPortaliP35525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996661.
STRINGi10116.ENSRNOP00000066524.

Protein family/group databases

TCDBi2.A.49.2.6. the chloride carrier/channel (clc) family.

PTM databases

iPTMnetiP35525.
PhosphoSiteiP35525.

Proteomic databases

PaxDbiP35525.
PRIDEiP35525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050927; ENSRNOP00000050925; ENSRNOG00000001742.
ENSRNOT00000071707; ENSRNOP00000066524; ENSRNOG00000050854.
GeneIDi100912455.
29232.
KEGGirno:100912455.
rno:29232.

Organism-specific databases

CTDi1181.
RGDi2361. Clcn2.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP35525.
KOiK05011.
OMAiESCEKRK.
OrthoDBiEOG091G01RJ.
PhylomeDBiP35525.
TreeFamiTF300522.

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiP35525.

Gene expression databases

BgeeiENSRNOG00000001742.
GenevisibleiP35525. RN.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR002244. Cl-channel-2.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01113. CLCHANNEL2.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN2_RAT
AccessioniPrimary (citable) accession number: P35525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.