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Protein

Chloride channel protein 1

Gene

Clcn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei189 – 1891ChlorideBy similarity
Binding sitei484 – 4841Chloride; via amide nitrogenBy similarity
Binding sitei578 – 5781ChlorideBy similarity

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • voltage-gated chloride channel activity Source: RGD

GO - Biological processi

  • chloride transport Source: RGD
  • muscle contraction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 1
Short name:
ClC-1
Alternative name(s):
Chloride channel protein, skeletal muscle
Gene namesi
Name:Clcn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2360. Clcn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 114114CytoplasmicBy similarityAdd
BLAST
Transmembranei115 – 15238HelicalBy similarityAdd
BLAST
Transmembranei159 – 18224HelicalBy similarityAdd
BLAST
Intramembranei191 – 1988HelicalBy similarity
Transmembranei207 – 22519HelicalBy similarityAdd
BLAST
Transmembranei232 – 25019HelicalBy similarityAdd
BLAST
Intramembranei266 – 27813HelicalBy similarityAdd
BLAST
Intramembranei282 – 2909HelicalBy similarity
Transmembranei302 – 32120HelicalBy similarityAdd
BLAST
Transmembranei348 – 37629HelicalBy similarityAdd
BLAST
Transmembranei385 – 40420HelicalBy similarityAdd
BLAST
Transmembranei454 – 47421HelicalBy similarityAdd
BLAST
Transmembranei482 – 50524HelicalBy similarityAdd
BLAST
Intramembranei522 – 53615HelicalBy similarityAdd
BLAST
Intramembranei537 – 5382Note=Loop between two helicesBy similarity
Intramembranei539 – 55012HelicalBy similarityAdd
BLAST
Intramembranei551 – 5555Note=Loop between two helicesBy similarity
Transmembranei556 – 57318HelicalBy similarityAdd
BLAST
Topological domaini574 – 994421CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 994994Chloride channel protein 1PRO_0000094431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei892 – 8921PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35524.
PRIDEiP35524.

PTM databases

iPTMnetiP35524.
PhosphoSiteiP35524.
SwissPalmiP35524.

Expressioni

Tissue specificityi

Predominantly expressed in skeletal muscles.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247717. 3 interactions.
STRINGi10116.ENSRNOP00000043372.

Chemistry

BindingDBiP35524.

Structurei

3D structure databases

ProteinModelPortaliP35524.
SMRiP35524. Positions 803-878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini609 – 66860CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini827 – 88256CBS 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi188 – 1925Selectivity filter part_1By similarity
Motifi230 – 2345Selectivity filter part_2By similarity
Motifi482 – 4865Selectivity filter part_3By similarity

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP35524.
KOiK05010.
PhylomeDBiP35524.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002243. Cl_channel-1.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01112. CLCHANNEL1.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSQSQQHG GEQSWWGTAP QYQYMPFEHC TSYGLPSENG GLQHRPRKDL
60 70 80 90 100
GPRHNAHPTQ IYGHHKEQYS YQAQDRGIPK KTDSSSTVDS LDEDHYSKCQ
110 120 130 140 150
DCVHRLGRVL RRKLGEDWIF LVLLGLLMAL VSWCMDYVSA KSLQAYKWTY
160 170 180 190 200
AQMQPSLPLQ YLAWVTFPLI LILFSALFCQ LISPQAVGSG IPEMKTILRG
210 220 230 240 250
VVLKEYLTLK AFVAKVVALT AGLGSGIPVG KEGPFVHIAS ICAAVLSKFM
260 270 280 290 300
SMFSGVYEQP YYYTDILTVG CAVGVGCCFG TPLGGVLFSI EVTSTYFAVR
310 320 330 340 350
NYWRGFFAAT FSAFVFRVLA VWNKDAVTIT ALFRTNFRMD FPFDLKELPA
360 370 380 390 400
FAVIGICCGF LGAVFVYLHR QVMLGVRKHK ALSQFLAKHR LLYPGIVTFV
410 420 430 440 450
IASLTFPPGM GQFMAGELMP REAISTLFDN NTWVKHIGDP KSLGQSAVWI
460 470 480 490 500
HPQVNVVIII LLFFVMKFWM SIVATTMPIP CGGFMPVFVL GAAFGRLVGE
510 520 530 540 550
IMAMLFPEGI LFDDIIYKIL PGGYAVIGAA ALTGAVSHTV STAVICFELT
560 570 580 590 600
GQIAHILPMM VAVILANMVA QSLQPSLYDS IIQVKKLPYL PDLGWNQLSK
610 620 630 640 650
FTIFVEDIMV RDVKFVSASC TYGELRNLLQ TTTVKTLPLV DSKDSMILLG
660 670 680 690 700
SVERSELQSL LQRHLCAERR LKAAQDMARK LSELPYNGQA QLAGEWHPGG
710 720 730 740 750
RPESFAFVDE DEDEDVSRKT ELPQTPTPPP PPPPPLPPQF PIAPSYPEEP
760 770 780 790 800
NGPLPSHKQP PEASDSADQR SSIFQRLLHC LLGKAHSTKK KITQDSTDLV
810 820 830 840 850
DNMSPEEIEA WEREQLSQPV CFDFCCIDQS PFQLVEQTTL HKTHTLFSLL
860 870 880 890 900
GLHLAYVTSM GKLRGVLALE ELQKAIKGHT KSGVQLRPPL ASFRNTTSIR
910 920 930 940 950
KTPGGPPPPA ESWNVPEGED GAPEREVMVP TMPETPVPPP SPEVPSCLAP
960 970 980 990
ARVEGELEEL EMVGNLGPEE DLADILHGPS LRSTDEEDED ELIL
Length:994
Mass (Da):110,073
Last modified:June 1, 1994 - v1
Checksum:iDCDDCD0D26E48FAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62894 mRNA. Translation: CAA44683.1.
PIRiS19595.
RefSeqiNP_037279.1. NM_013147.1.
UniGeneiRn.10440.

Genome annotation databases

GeneIDi25688.
KEGGirno:25688.
UCSCiRGD:2360. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62894 mRNA. Translation: CAA44683.1.
PIRiS19595.
RefSeqiNP_037279.1. NM_013147.1.
UniGeneiRn.10440.

3D structure databases

ProteinModelPortaliP35524.
SMRiP35524. Positions 803-878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247717. 3 interactions.
STRINGi10116.ENSRNOP00000043372.

Chemistry

BindingDBiP35524.

PTM databases

iPTMnetiP35524.
PhosphoSiteiP35524.
SwissPalmiP35524.

Proteomic databases

PaxDbiP35524.
PRIDEiP35524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25688.
KEGGirno:25688.
UCSCiRGD:2360. rat.

Organism-specific databases

CTDi1180.
RGDi2360. Clcn1.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP35524.
KOiK05010.
PhylomeDBiP35524.

Miscellaneous databases

PROiP35524.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002243. Cl_channel-1.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01112. CLCHANNEL1.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel."
    Steinmeyer K., Ortland C., Jentsch T.J.
    Nature 354:301-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-892, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLCN1_RAT
AccessioniPrimary (citable) accession number: P35524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.