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P35516 (MTL1_LACLL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase LlaI

Short name=M.LlaI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase LlaI
Gene names
Name:llaIM
Encoded onPlasmid pTR2030
OrganismLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifier1360 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylation of specific adenine residues; required for both restriction and modification activities. May recognize a non-palindromic sequence.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
   Biological processRestriction system
   DomainRepeat
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

site-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Modification methylase LlaI
PRO_0000087961

Sequences

Sequence LengthMass (Da)Tools
P35516 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 69A817F46BE9C772

FASTA62272,513
        10         20         30         40         50         60 
MRYLGNKTNL LNFIQQVIKK HDIQGQTFAD LFAGTGSVGD YFKGEYTVLS NDYMYFSKVI 

        70         80         90        100        110        120 
SEAKLLNSEK PKFDSFVKRY GKTPFQWLNE REYTPNDGYF VYNNYTPRAE RMYLTEENAL 

       130        140        150        160        170        180 
KIDGMRLDIE ELFQEGVISK AEYSYLLASL LESVTKVSNT SGTYQAFFKF WESRALKKFT 

       190        200        210        220        230        240 
IMPLEMKDSL SVSKDNRCFN KNTNRLVREI SGDIAYIDPP YTITQYTNSY HVLETIARYD 

       250        260        270        280        290        300 
NPELFGKTGR RVKREFSGYS NKSKAYYEFE DLFRQINFTH VLVSYSNQSI VPLDELVDLA 

       310        320        330        340        350        360 
RRFAVDGIVE VETNEYREYS TNNSSMKGEG KKLQEVIIYF KKNLETNKSP LNYAGSKDDV 

       370        380        390        400        410        420 
IPRIFKLLPK HVTTFVDAMG GAFNVGANRT ALNKVVYNEY HPFVFEMMQM IVNTPADELI 

       430        440        450        460        470        480 
RNVEQIVTRY SLEKKGKEAF NRLRDHYNNE EQTPINLYTL NIYSFQNILR FNQAKKYNTP 

       490        500        510        520        530        540 
IGNNEFNEGY KDRITRFVTR APEVEMRLGS YSAINFNEYD DDTVFYFDPP YLVTTAGYND 

       550        560        570        580        590        600 
GKRGFDGWDA EQEASLLKYL TELDSAGKKF MLSNVLEHKG KTNHLLMEWI QHHGFNVNTI 

       610        620 
GETGIKYPRR EILVTNYNTF ER 

« Hide

References

[1]"In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage."
Hill C., Miller L.A., Klaenhammer T.R.
J. Bacteriol. 173:4363-4370(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ME2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17233 Genomic DNA. Translation: AAA65073.1.
PIRS35122.

3D structure databases

ProteinModelPortalP35516.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3437. M.LlaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProIPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFPIRSF036638. M_m6A_StsI. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 3 hits.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTL1_LACLL
AccessionPrimary (citable) accession number: P35516
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries