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P35516

- MTL1_LACLL

UniProt

P35516 - MTL1_LACLL

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Protein

Modification methylase LlaI

Gene

llaIM

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Methylation of specific adenine residues; required for both restriction and modification activities. May recognize a non-palindromic sequence.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3437. M.LlaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase LlaI (EC:2.1.1.72)
Short name:
M.LlaI
Alternative name(s):
Adenine-specific methyltransferase LlaI
Gene namesi
Name:llaIM
Encoded oniPlasmid pTR20300 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Modification methylase LlaIPRO_0000087961Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP35516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProiIPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF036638. M_m6A_StsI. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35516-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYLGNKTNL LNFIQQVIKK HDIQGQTFAD LFAGTGSVGD YFKGEYTVLS
60 70 80 90 100
NDYMYFSKVI SEAKLLNSEK PKFDSFVKRY GKTPFQWLNE REYTPNDGYF
110 120 130 140 150
VYNNYTPRAE RMYLTEENAL KIDGMRLDIE ELFQEGVISK AEYSYLLASL
160 170 180 190 200
LESVTKVSNT SGTYQAFFKF WESRALKKFT IMPLEMKDSL SVSKDNRCFN
210 220 230 240 250
KNTNRLVREI SGDIAYIDPP YTITQYTNSY HVLETIARYD NPELFGKTGR
260 270 280 290 300
RVKREFSGYS NKSKAYYEFE DLFRQINFTH VLVSYSNQSI VPLDELVDLA
310 320 330 340 350
RRFAVDGIVE VETNEYREYS TNNSSMKGEG KKLQEVIIYF KKNLETNKSP
360 370 380 390 400
LNYAGSKDDV IPRIFKLLPK HVTTFVDAMG GAFNVGANRT ALNKVVYNEY
410 420 430 440 450
HPFVFEMMQM IVNTPADELI RNVEQIVTRY SLEKKGKEAF NRLRDHYNNE
460 470 480 490 500
EQTPINLYTL NIYSFQNILR FNQAKKYNTP IGNNEFNEGY KDRITRFVTR
510 520 530 540 550
APEVEMRLGS YSAINFNEYD DDTVFYFDPP YLVTTAGYND GKRGFDGWDA
560 570 580 590 600
EQEASLLKYL TELDSAGKKF MLSNVLEHKG KTNHLLMEWI QHHGFNVNTI
610 620
GETGIKYPRR EILVTNYNTF ER
Length:622
Mass (Da):72,513
Last modified:June 1, 1994 - v1
Checksum:i69A817F46BE9C772
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17233 Genomic DNA. Translation: AAA65073.1.
PIRiS35122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17233 Genomic DNA. Translation: AAA65073.1 .
PIRi S35122.

3D structure databases

ProteinModelPortali P35516.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3437. M.LlaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProi IPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 2 hits.
[Graphical view ]
PIRSFi PIRSF036638. M_m6A_StsI. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage."
    Hill C., Miller L.A., Klaenhammer T.R.
    J. Bacteriol. 173:4363-4370(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ME2.

Entry informationi

Entry nameiMTL1_LACLL
AccessioniPrimary (citable) accession number: P35516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3