P35516 (MTL1_LACLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase LlaI
Short name=M.LlaI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase LlaI

Gene names

Name:

llaIM

Encoded on

Plasmid pTR2030

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylation of specific adenine residues; required for both restriction and modification activities. May recognize a non-palindromic sequence.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Modification methylase LlaI		PRO_0000087961

Sequences

	Sequence		Length	Mass (Da)
	P35516 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 69A817F46BE9C772	FASTA	622	72,513
	10 20 30 40 50 60 MRYLGNKTNL LNFIQQVIKK HDIQGQTFAD LFAGTGSVGD YFKGEYTVLS NDYMYFSKVI 70 80 90 100 110 120 SEAKLLNSEK PKFDSFVKRY GKTPFQWLNE REYTPNDGYF VYNNYTPRAE RMYLTEENAL 130 140 150 160 170 180 KIDGMRLDIE ELFQEGVISK AEYSYLLASL LESVTKVSNT SGTYQAFFKF WESRALKKFT 190 200 210 220 230 240 IMPLEMKDSL SVSKDNRCFN KNTNRLVREI SGDIAYIDPP YTITQYTNSY HVLETIARYD 250 260 270 280 290 300 NPELFGKTGR RVKREFSGYS NKSKAYYEFE DLFRQINFTH VLVSYSNQSI VPLDELVDLA 310 320 330 340 350 360 RRFAVDGIVE VETNEYREYS TNNSSMKGEG KKLQEVIIYF KKNLETNKSP LNYAGSKDDV 370 380 390 400 410 420 IPRIFKLLPK HVTTFVDAMG GAFNVGANRT ALNKVVYNEY HPFVFEMMQM IVNTPADELI 430 440 450 460 470 480 RNVEQIVTRY SLEKKGKEAF NRLRDHYNNE EQTPINLYTL NIYSFQNILR FNQAKKYNTP 490 500 510 520 530 540 IGNNEFNEGY KDRITRFVTR APEVEMRLGS YSAINFNEYD DDTVFYFDPP YLVTTAGYND 550 560 570 580 590 600 GKRGFDGWDA EQEASLLKYL TELDSAGKKF MLSNVLEHKG KTNHLLMEWI QHHGFNVNTI 610 620 GETGIKYPRR EILVTNYNTF ER

References

[1]

"In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage." Hill C., Miller L.A., Klaenhammer T.R. J. Bacteriol. 173:4363-4370(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ME2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17233 Genomic DNA. Translation: AAA65073.1.
PIR	S35122.
3D structure databases
ProteinModelPortal	P35516.
ModBase	Search...
Protein family/group databases
REBASE	3437. M.LlaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1020.10. 1 hit.
InterPro	IPR012186. Ade-mod_methylase_MStsI. IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 2 hits. [Graphical view]
PIRSF	PIRSF036638. M_m6A_StsI. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTL1_LACLL

Accession

Primary (citable) accession number: P35516

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program