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P35516

- MTL1_LACLL

UniProt

P35516 - MTL1_LACLL

Protein

Modification methylase LlaI

Gene

llaIM

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Methylation of specific adenine residues; required for both restriction and modification activities. May recognize a non-palindromic sequence.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3437. M.LlaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase LlaI (EC:2.1.1.72)
    Short name:
    M.LlaI
    Alternative name(s):
    Adenine-specific methyltransferase LlaI
    Gene namesi
    Name:llaIM
    Encoded oniPlasmid pTR20300 Publication
    OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
    Taxonomic identifieri1360 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Modification methylase LlaIPRO_0000087961Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP35516.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 4 hits.
    InterProiIPR012186. Ade-mod_methylase_MStsI.
    IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 2 hits.
    [Graphical view]
    PIRSFiPIRSF036638. M_m6A_StsI. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 3 hits.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35516-1 [UniParc]FASTAAdd to Basket

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    MRYLGNKTNL LNFIQQVIKK HDIQGQTFAD LFAGTGSVGD YFKGEYTVLS    50
    NDYMYFSKVI SEAKLLNSEK PKFDSFVKRY GKTPFQWLNE REYTPNDGYF 100
    VYNNYTPRAE RMYLTEENAL KIDGMRLDIE ELFQEGVISK AEYSYLLASL 150
    LESVTKVSNT SGTYQAFFKF WESRALKKFT IMPLEMKDSL SVSKDNRCFN 200
    KNTNRLVREI SGDIAYIDPP YTITQYTNSY HVLETIARYD NPELFGKTGR 250
    RVKREFSGYS NKSKAYYEFE DLFRQINFTH VLVSYSNQSI VPLDELVDLA 300
    RRFAVDGIVE VETNEYREYS TNNSSMKGEG KKLQEVIIYF KKNLETNKSP 350
    LNYAGSKDDV IPRIFKLLPK HVTTFVDAMG GAFNVGANRT ALNKVVYNEY 400
    HPFVFEMMQM IVNTPADELI RNVEQIVTRY SLEKKGKEAF NRLRDHYNNE 450
    EQTPINLYTL NIYSFQNILR FNQAKKYNTP IGNNEFNEGY KDRITRFVTR 500
    APEVEMRLGS YSAINFNEYD DDTVFYFDPP YLVTTAGYND GKRGFDGWDA 550
    EQEASLLKYL TELDSAGKKF MLSNVLEHKG KTNHLLMEWI QHHGFNVNTI 600
    GETGIKYPRR EILVTNYNTF ER 622
    Length:622
    Mass (Da):72,513
    Last modified:June 1, 1994 - v1
    Checksum:i69A817F46BE9C772
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17233 Genomic DNA. Translation: AAA65073.1.
    PIRiS35122.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17233 Genomic DNA. Translation: AAA65073.1 .
    PIRi S35122.

    3D structure databases

    ProteinModelPortali P35516.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3437. M.LlaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 4 hits.
    InterProi IPR012186. Ade-mod_methylase_MStsI.
    IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF036638. M_m6A_StsI. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 3 hits.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage."
      Hill C., Miller L.A., Klaenhammer T.R.
      J. Bacteriol. 173:4363-4370(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ME2.

    Entry informationi

    Entry nameiMTL1_LACLL
    AccessioniPrimary (citable) accession number: P35516
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3