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Protein

Major seminal plasma glycoprotein PSP-II

Gene
N/A
Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Major seminal plasma glycoprotein PSP-II
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 137116Major seminal plasma glycoprotein PSP-IIPRO_0000033189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 51PROSITE-ProRule annotation1 Publication
Disulfide bondi74 ↔ 95PROSITE-ProRule annotation1 Publication
Glycosylationi119 – 1191N-linked (GlcNAc...) (complex)1 PublicationCAR_000148

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35496.

PTM databases

UniCarbKBiP35496.

Expressioni

Tissue specificityi

Seminal plasma or sperm.

Interactioni

Subunit structurei

Monomer or heterodimer with PSP-I (depending on the type of glycosylation of PSP-I).

Protein-protein interaction databases

MINTiMINT-1528244.
STRINGi9823.ENSSSCP00000003225.

Structurei

Secondary structure

1
137
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 253Combined sources
Helixi26 – 294Combined sources
Beta strandi32 – 343Combined sources
Beta strandi37 – 426Combined sources
Beta strandi49 – 568Combined sources
Beta strandi62 – 7110Combined sources
Beta strandi77 – 859Combined sources
Beta strandi90 – 10314Combined sources
Beta strandi105 – 11713Combined sources
Beta strandi125 – 1317Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SPPX-ray2.40B22-137[»]
ProteinModelPortaliP35496.
SMRiP35496. Positions 22-133.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35496.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 131102CUBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the spermadhesin family.Curated
Contains 1 CUB domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JGFV. Eukaryota.
ENOG41118AQ. LUCA.
HOGENOMiHOG000168451.
HOVERGENiHBG004277.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR000124. Spermadhesin.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00985. SPERMADHESIN_1. 1 hit.
PS00986. SPERMADHESIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLGTAIPWA LLLSTATLVS TARINGPDEC GRVIKDTSGS ISNTDRQKNL
60 70 80 90 100
CTWTILMKPD QKVRMAIPYL NLACGKEYVE VFDGLLSGPS YGKLCAGAAI
110 120 130
VFLSTANTMT IKYNRISGNS SSPFLIYFYG SSPGSEY
Length:137
Mass (Da):14,816
Last modified:February 1, 1996 - v2
Checksum:iFD4C26A97DAF5D23
GO

Mass spectrometryi

Molecular mass is 12410±8 Da from positions 22 - 137. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02627 mRNA. Translation: AAC48400.1.
PIRiS65875.
RefSeqiNP_999001.1. NM_213836.1.
UniGeneiSsc.94425.

Genome annotation databases

GeneIDi396817.
KEGGissc:396817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02627 mRNA. Translation: AAC48400.1.
PIRiS65875.
RefSeqiNP_999001.1. NM_213836.1.
UniGeneiSsc.94425.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SPPX-ray2.40B22-137[»]
ProteinModelPortaliP35496.
SMRiP35496. Positions 22-133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1528244.
STRINGi9823.ENSSSCP00000003225.

PTM databases

UniCarbKBiP35496.

Proteomic databases

PaxDbiP35496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396817.
KEGGissc:396817.

Organism-specific databases

CTDi396817.

Phylogenomic databases

eggNOGiENOG410JGFV. Eukaryota.
ENOG41118AQ. LUCA.
HOGENOMiHOG000168451.
HOVERGENiHBG004277.

Miscellaneous databases

EvolutionaryTraceiP35496.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR000124. Spermadhesin.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00985. SPERMADHESIN_1. 1 hit.
PS00986. SPERMADHESIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and sequence analysis of two porcine seminal proteins, PSP-I and PSP-II: new members of the spermadhesin family."
    Kwok S.C.M., Yang D., Dai G., Soares M.J., Chen S., McMurtry J.P.
    DNA Cell Biol. 12:605-610(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Seminal vesicle.
  2. "Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasma."
    Rutherfurd K.J., Swiderek K.M., Green C.B., Chen S., Shively J.E., Kwok S.C.M.
    Arch. Biochem. Biophys. 295:352-359(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-34.
    Tissue: Sperm.
  3. "Boar spermadhesin PSP-II: location of posttranslational modifications, heterodimer formation with PSP-I glycoforms and effect of dimerization on the ligand-binding capabilities of the subunits."
    Calvete J.J., Mann K., Schaefer W., Raida M., Sanz L., Toepfer-Petersen E.
    FEBS Lett. 365:179-182(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-119, MASS SPECTROMETRY.
    Tissue: Sperm.
  4. "Structural characterization of the oligosaccharide chains of native and crystallized boar seminal plasma spermadhesin PSP-I and PSP-II glycoforms."
    Nimtz M., Grabenhorst E., Conradt H.S., Sanz L., Calvete J.J.
    Eur. J. Biochem. 265:703-718(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE OF CARBOHYDRATE.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiPSP2_PIG
AccessioniPrimary (citable) accession number: P35496
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.