P35493 (PMGI_RICCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase Short name=BPG-independent PGAM Short name=Phosphoglyceromutase EC=5.4.2.1 Alternative name(s): PGAM-I
Organism	Ricinus communis (Castor bean) [Complete proteome]
Taxonomic identifier	3988 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate By similarity.
Catalytic activity	2-phospho-D-glycerate = 3-phospho-D-glycerate.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Found ubiquitously in germinating seed.
Developmental stage	Expression most important during germination, it decreases during development.
Sequence similarities	Belongs to the BPG-independent phosphoglycerate mutase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	manganese ion binding Inferred from electronic annotation. Source: InterPro phosphoglycerate mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	2,3-bisphosphoglycerate-independent phosphoglycerate mutase		PRO_0000212113
Sites
	Active site	78	1	Phosphoserine intermediate By similarity
	Metal binding	25	1	Manganese 2 By similarity
	Metal binding	78	1	Manganese 2 By similarity
	Metal binding	427	1	Manganese 1 By similarity
	Metal binding	431	1	Manganese 1 By similarity
	Metal binding	468	1	Manganese 2 By similarity
	Metal binding	469	1	Manganese 2 By similarity
	Metal binding	498	1	Manganese 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35493 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: F6456D7D8067862C	FASTA	556	60,818
	10 20 30 40 50 60 GEFTWKLADH PKLPKGKTIA MVVLDGWGEA KPDQYNCIHV AETPTMDSFK KTAPERWRLI 70 80 90 100 110 120 KAHGTAVGLP TEDDMGNSEV GHNALGAGRI YAQGAKLVDL ALASGKIYEG EGFKYVKECF 130 140 150 160 170 180 DKGTLHLIGL LSDGGVHSRL DQLQLLLKGA AEHGAKRIRV HVLTDGRDVI DGTSVGFAET 190 200 210 220 230 240 LEKDLENLRE KGVDAQVASG GGRMYVTMDR YENDWNVVKR GWDAQVLGEA PYKFKSAVEA 250 260 270 280 290 300 IKKLREEPKA NDQYLPPFVI VDENGKPVGP IVDGDAVVTI NFRADRMVML AKALEYENFD 310 320 330 340 350 360 TFDRVRFPKI HYAGMLQYDG ELKLPSHYLV SPPEIERTSG EYLVHNGVHT FACSETVKFG 370 380 390 400 410 420 HVTFFWNGNR SGYFNPEMEE YVEIPSDVGI TFNVQPKMKA IEIAEKARDA ILSGKFQQVR 430 440 450 460 470 480 VNIPNGDMVG HTGDVEATVV GCKAADEAVK MIIDAIEQVG GIYVVTADHG NAEDMVKRDK 490 500 510 520 530 540 SGKPMADKSG KIQILTSHTL QPVPIAIGGP GLTPGVRFRS DIPTGGLANV AATVMNLHGF 550 EAPSDYEPTL IEAVDN

References

[1]

"Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression." Huang Y., Blakeley S.D., McAleese S.M., Fothergill-Gilmore L.A., Dennis D.T. Plant Mol. Biol. 23:1039-1053(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-12. Strain: cv. Baker 296. Tissue: Endosperm.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70652 mRNA. Translation: CAA49995.1.
PIR	S49647.
3D structure databases
ProteinModelPortal	P35493.
ModBase	Search...
Proteomic databases
ProMEX	P35493.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os03t0330200-00.
Enzyme and pathway databases
UniPathway	UPA00109; UER00186.
Family and domain databases
Gene3D	3.40.1450.10. 1 hit. 3.40.720.10. 2 hits.
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR011258. BPG-indep_PGM_N. IPR006124. Metalloenzyme. IPR005995. Pgm_bpd_ind. [Graphical view]
Pfam	PF06415. iPGM_N. 1 hit. PF01676. Metalloenzyme. 1 hit. [Graphical view]
PIRSF	PIRSF001492. IPGAM. 1 hit.
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit. SSF64158. BPG-indep_PGM_N. 1 hit.
TIGRFAMs	TIGR01307. pgm_bpd_ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PMGI_RICCO

Accession

Primary (citable) accession number: P35493

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program