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P35487

- ODPAT_MOUSE

UniProt

P35487 - ODPAT_MOUSE

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Protein
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
Gene
Pdha2, Pdha-2, Pdhal
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle By similarity.

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Thiamine pyrophosphate By similarity.

Enzyme regulationi

Pyruvate dehydrogenase activity is inhibited by phosphorylation of PDHA2; it is reactivated by dephosphorylation By similarity.

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
  2. glycolytic process Source: InterPro
  3. pyruvate metabolic process Source: UniProtKB
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (EC:1.2.4.1)
Alternative name(s):
PDHE1-A type II
Gene namesi
Name:Pdha2
Synonyms:Pdha-2, Pdhal
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:97533. Pdha2.

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030Mitochondrion By similarity
Add
BLAST
Chaini31 – 391361Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
PRO_0000020448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei294 – 2941Phosphoserine; by PDK1, PDK2, PDK3 and PDK4 By similarity
Modified residuei301 – 3011Phosphoserine; by PDK3 By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35487.
PRIDEiP35487.

2D gel databases

REPRODUCTION-2DPAGEP35487.

PTM databases

PhosphoSiteiP35487.

Expressioni

Tissue specificityi

Testis.

Gene expression databases

BgeeiP35487.
CleanExiMM_PDHA2.
GenevestigatoriP35487.

Interactioni

Subunit structurei

Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules By similarity.

Structurei

3D structure databases

ProteinModelPortaliP35487.
SMRiP35487. Positions 31-391.

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1071.
GeneTreeiENSGT00530000063174.
HOGENOMiHOG000281336.
HOVERGENiHBG001863.
InParanoidiQ497M8.
KOiK00161.
OMAiTSNERSA.
OrthoDBiEOG73JKVQ.
PhylomeDBiP35487.
TreeFamiTF300742.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
TIGRFAMsiTIGR03182. PDH_E1_alph_y. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35487-1 [UniParc]FASTAAdd to Basket

« Hide

MRKMLTAVLS HVFSGMVQKP ALRGLLSSLK FSNDATCDIK KCDLYRLEEG    50
PPTSTVLTRA EALKYYRTMQ VIRRMELKAD QLYKQKFIRG FCHLCDGQEA 100
CCVGLEAGIN PTDHVITSYR AHGFCYTRGL SVKSILAELT GRKGGCAKGK 150
GGSMHMYGKN FYGGNGIVGA QVPLGAGVAF ACKYLKNGQV CLALYGDGAA 200
NQGQVFEAYN MSALWKLPCV FICENNLYGM GTSNERSAAS TDYHKKGFII 250
PGLRVNGMDI LCVREATKFA ADHCRSGKGP IVMELQTYRY HGHSMSDPGI 300
SYRSREEVHN VRSKSDPIML LRERIISNNL SNIEELKEID ADVKKEVEDA 350
AQFATTDPEP AVEDIANYLY HQDPPFEVRG AHKWLKYKSH S 391
Length:391
Mass (Da):43,413
Last modified:June 1, 1994 - v1
Checksum:iA2DE823362485977
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76728 mRNA. Translation: AAA53047.1.
AK076791 mRNA. Translation: BAC36482.1.
BC100460 mRNA. Translation: AAI00461.1.
CCDSiCCDS17872.1.
PIRiS23507.
RefSeqiNP_032837.1. NM_008811.2.
UniGeneiMm.4223.

Genome annotation databases

EnsembliENSMUST00000057860; ENSMUSP00000060774; ENSMUSG00000047674.
GeneIDi18598.
KEGGimmu:18598.
UCSCiuc008rob.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76728 mRNA. Translation: AAA53047.1 .
AK076791 mRNA. Translation: BAC36482.1 .
BC100460 mRNA. Translation: AAI00461.1 .
CCDSi CCDS17872.1.
PIRi S23507.
RefSeqi NP_032837.1. NM_008811.2.
UniGenei Mm.4223.

3D structure databases

ProteinModelPortali P35487.
SMRi P35487. Positions 31-391.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P35487.

2D gel databases

REPRODUCTION-2DPAGE P35487.

Proteomic databases

PaxDbi P35487.
PRIDEi P35487.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000057860 ; ENSMUSP00000060774 ; ENSMUSG00000047674 .
GeneIDi 18598.
KEGGi mmu:18598.
UCSCi uc008rob.1. mouse.

Organism-specific databases

CTDi 5161.
MGIi MGI:97533. Pdha2.

Phylogenomic databases

eggNOGi COG1071.
GeneTreei ENSGT00530000063174.
HOGENOMi HOG000281336.
HOVERGENi HBG001863.
InParanoidi Q497M8.
KOi K00161.
OMAi TSNERSA.
OrthoDBi EOG73JKVQ.
PhylomeDBi P35487.
TreeFami TF300742.

Miscellaneous databases

NextBioi 294494.
PROi P35487.
SOURCEi Search...

Gene expression databases

Bgeei P35487.
CleanExi MM_PDHA2.
Genevestigatori P35487.

Family and domain databases

Gene3Di 3.40.50.970. 1 hit.
InterProi IPR001017. DH_E1.
IPR017597. Pyrv_DH_E1_asu_subgrp-y.
IPR029061. THDP-binding.
[Graphical view ]
Pfami PF00676. E1_dh. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 1 hit.
TIGRFAMsi TIGR03182. PDH_E1_alph_y. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterisation of the mouse pyruvate dehydrogenase E1 alpha genes."
    Fitzgerald G.F., Hutchison W.M., Dahl H.-H.M.
    Biochim. Biophys. Acta 1131:83-90(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiODPAT_MOUSE
AccessioniPrimary (citable) accession number: P35487
Secondary accession number(s): Q497M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

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