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P35483

- PPBH_PSEAE

UniProt

P35483 - PPBH_PSEAE

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Protein

Alkaline phosphatase H

Gene

phoA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Has only phosphomonoesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg(2+) ion per subunit
  • Zn2+Note: Binds 2 Zn(2+) ions per subunit

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771MagnesiumBy similarity
Metal bindingi77 – 771Zinc 2By similarity
Active sitei128 – 1281Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi179 – 1791MagnesiumBy similarity
Metal bindingi181 – 1811MagnesiumBy similarity
Metal bindingi346 – 3461MagnesiumBy similarity
Metal bindingi353 – 3531Zinc 1By similarity
Metal bindingi357 – 3571Zinc 1By similarity
Metal bindingi395 – 3951Zinc 2By similarity
Metal bindingi396 – 3961Zinc 2By similarity
Metal bindingi438 – 4381Zinc 1By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase H (EC:3.1.3.1)
Alternative name(s):
High molecular weight phosphatase1 Publication
Short name:
H-AP
Gene namesi
Name:phoA
Ordered Locus Names:PA3296
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3296.

Subcellular locationi

Secreted 1 Publication. Periplasm 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 476450Alkaline phosphatase HPRO_0000024014Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed constitutively.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3296.

Structurei

3D structure databases

ProteinModelPortaliP35483.
SMRiP35483. Positions 31-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099117.
InParanoidiP35483.
KOiK01077.
OMAiDKGFFLQ.
OrthoDBiEOG661H4G.
PhylomeDBiP35483.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35483-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR
60 70 80 90 100
VEQDLTQALK QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG
110 120 130 140 150
IDALPLTGQY THYSLHKDSG LPDYVTDSAA SATAWSTGVK SYNGAIGVDI
160 170 180 190 200
HEQPHRNLLE LAKLNGKATG NVSTAELQDA TPAALLAHVT ARKCYGPEAT
210 220 230 240 250
SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET AKAGRYAGKT
260 270 280 290 300
LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT
310 320 330 340 350
YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA
360 370 380 390 400
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS
410 420 430 440 450
QIIPPETAAP GLTQLLTTKD GAPLAISYGN SEESSQEHTG TQLRIAAYGP
460 470
QAANVTGLTD QTDLFFTIRR ALNLRD
Length:476
Mass (Da):50,409
Last modified:January 11, 2001 - v2
Checksum:iA3059B30BBEA809C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271R → Q AA sequence (PubMed:8454193)Curated
Sequence conflicti39 – 391R → A AA sequence (PubMed:8454193)Curated
Sequence conflicti44 – 452EY → VR AA sequence (PubMed:8454193)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06684.1.
PIRiA83235.
RefSeqiNP_251986.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06684; AAG06684; PA3296.
GeneIDi882459.
KEGGipae:PA3296.
PATRICi19841179. VBIPseAer58763_3448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06684.1 .
PIRi A83235.
RefSeqi NP_251986.1. NC_002516.2.

3D structure databases

ProteinModelPortali P35483.
SMRi P35483. Positions 31-475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06684 ; AAG06684 ; PA3296 .
GeneIDi 882459.
KEGGi pae:PA3296.
PATRICi 19841179. VBIPseAer58763_3448.

Organism-specific databases

PseudoCAPi PA3296.

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099117.
InParanoidi P35483.
KOi K01077.
OMAi DKGFFLQ.
OrthoDBi EOG661H4G.
PhylomeDBi P35483.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
    Tan A.S.P., Worobec E.A.
    FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-45, FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
    Strain: H103.

Entry informationi

Entry nameiPPBH_PSEAE
AccessioniPrimary (citable) accession number: P35483
Secondary accession number(s): Q9HYU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: November 26, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3