Reviewed, UniProtKB/Swiss-Prot P35483 (PPBH_PSEAE)

Names and origin

Protein names

Recommended name:
    Alkaline phosphatase H
    EC=3.1.3.1
Alternative name(s):
    High molecular weight phosphatase
      Short name=H-AP

Gene names

Name:	phoA
Ordered Locus Names:	PA3296

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has only a phosphomonoesterase activity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion. Binds 2 zinc ions.
Subcellular location	Secreted. Periplasm.
Developmental stage	Expressed constitutively.
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Periplasm Secreted
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.2
	Chain	27 – 476	450	Alkaline phosphatase H		PRO_0000024014
Sites
	Active site	128	1	Phosphoserine intermediate By similarity
	Metal binding	77	1	Magnesium By similarity
	Metal binding	77	1	Zinc 2 By similarity
	Metal binding	179	1	Magnesium By similarity
	Metal binding	181	1	Magnesium By similarity
	Metal binding	346	1	Magnesium By similarity
	Metal binding	353	1	Zinc 1 By similarity
	Metal binding	357	1	Zinc 1 By similarity
	Metal binding	395	1	Zinc 2 By similarity
	Metal binding	396	1	Zinc 2 By similarity
	Metal binding	438	1	Zinc 1 By similarity
Experimental info
	Sequence conflict	27	1	R → Q AA sequence Ref.2
	Sequence conflict	39	1	R → A AA sequence Ref.2
	Sequence conflict	44 – 45	2	EY → VR AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35483-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: A3059B30BBEA809C	FASTA	476	50,409
	10 20 30 40 50 60 MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR VEQDLTQALK 70 80 90 100 110 120 QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG IDALPLTGQY THYSLHKDSG 130 140 150 160 170 180 LPDYVTDSAA SATAWSTGVK SYNGAIGVDI HEQPHRNLLE LAKLNGKATG NVSTAELQDA 190 200 210 220 230 240 TPAALLAHVT ARKCYGPEAT SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET 250 260 270 280 290 300 AKAGRYAGKT LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT 310 320 330 340 350 360 YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA SIDKQDHAAN 370 380 390 400 410 420 PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS QIIPPETAAP GLTQLLTTKD 430 440 450 460 470 GAPLAISYGN SEESSQEHTG TQLRIAAYGP QAANVTGLTD QTDLFFTIRR ALNLRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa." Tan A.S.P., Worobec E.A. FEMS Microbiol. Lett. 106:281-286(1993) [PubMed: 8454193] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-45. Strain: H103.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG06684.1.
PIR	A83235.
RefSeq	NP_251986.1.
3D structure databases
HSSP	HSSP built from PDB template 1AJC based on UniProtKB P00634.
SMR	P35483. Positions 31-475.
ModBase	Search...
Genome annotation databases
GeneID	882459.
GenomeReviews	Gene locus PA3296 in contig AE004091_GR.
KEGG	pae:PA3296.
NMPDR	fig|208964.1.peg.3296.
Organism-specific databases
PseudoCAP	PA3296.
CMR	Search...
Phylogenomic databases
HOGENOM	P35483.
OMA	VKQSTIA.
Enzyme and pathway databases
BioCyc	PAER208964:PA3296-MON.
BRENDA	3.1.3.1. 354.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPBH_PSEAE

Accession

Primary (citable) accession number: P35483
Secondary accession number(s): Q9HYU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 11, 2001
Last modified:	November 3, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)