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P35483

- PPBH_PSEAE

UniProt

P35483 - PPBH_PSEAE

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Protein
Alkaline phosphatase H
Gene
phoA, PA3296
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Has only a phosphomonoesterase activity.

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion.
Binds 2 zinc ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Magnesium By similarity
Metal bindingi77 – 771Zinc 2 By similarity
Active sitei128 – 1281Phosphoserine intermediate By similarity
Metal bindingi179 – 1791Magnesium By similarity
Metal bindingi181 – 1811Magnesium By similarity
Metal bindingi346 – 3461Magnesium By similarity
Metal bindingi353 – 3531Zinc 1 By similarity
Metal bindingi357 – 3571Zinc 1 By similarity
Metal bindingi395 – 3951Zinc 2 By similarity
Metal bindingi396 – 3961Zinc 2 By similarity
Metal bindingi438 – 4381Zinc 1 By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase H (EC:3.1.3.1)
Alternative name(s):
High molecular weight phosphatase
Short name:
H-AP
Gene namesi
Name:phoA
Ordered Locus Names:PA3296
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3296.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 Publication
Add
BLAST
Chaini27 – 476450Alkaline phosphatase H
PRO_0000024014Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed constitutively.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3296.

Structurei

3D structure databases

ProteinModelPortaliP35483.
SMRiP35483. Positions 33-474.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099117.
KOiK01077.
OMAiDKGFFLQ.
OrthoDBiEOG661H4G.
PhylomeDBiP35483.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35483-1 [UniParc]FASTAAdd to Basket

« Hide

MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR    50
VEQDLTQALK QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG 100
IDALPLTGQY THYSLHKDSG LPDYVTDSAA SATAWSTGVK SYNGAIGVDI 150
HEQPHRNLLE LAKLNGKATG NVSTAELQDA TPAALLAHVT ARKCYGPEAT 200
SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET AKAGRYAGKT 250
LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT 300
YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA 350
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS 400
QIIPPETAAP GLTQLLTTKD GAPLAISYGN SEESSQEHTG TQLRIAAYGP 450
QAANVTGLTD QTDLFFTIRR ALNLRD 476
Length:476
Mass (Da):50,409
Last modified:January 11, 2001 - v2
Checksum:iA3059B30BBEA809C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271R → Q AA sequence 1 Publication
Sequence conflicti39 – 391R → A AA sequence 1 Publication
Sequence conflicti44 – 452EY → VR AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG06684.1.
PIRiA83235.
RefSeqiNP_251986.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06684; AAG06684; PA3296.
GeneIDi882459.
KEGGipae:PA3296.
PATRICi19841179. VBIPseAer58763_3448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG06684.1 .
PIRi A83235.
RefSeqi NP_251986.1. NC_002516.2.

3D structure databases

ProteinModelPortali P35483.
SMRi P35483. Positions 33-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06684 ; AAG06684 ; PA3296 .
GeneIDi 882459.
KEGGi pae:PA3296.
PATRICi 19841179. VBIPseAer58763_3448.

Organism-specific databases

PseudoCAPi PA3296.

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099117.
KOi K01077.
OMAi DKGFFLQ.
OrthoDBi EOG661H4G.
PhylomeDBi P35483.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
    Tan A.S.P., Worobec E.A.
    FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-45.
    Strain: H103.

Entry informationi

Entry nameiPPBH_PSEAE
AccessioniPrimary (citable) accession number: P35483
Secondary accession number(s): Q9HYU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi