Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P35483

- PPBH_PSEAE

UniProt

P35483 - PPBH_PSEAE

Protein

Alkaline phosphatase H

Gene

phoA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Has only a phosphomonoesterase activity.

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.
    Binds 2 zinc ions.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771MagnesiumBy similarity
    Metal bindingi77 – 771Zinc 2By similarity
    Active sitei128 – 1281Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi179 – 1791MagnesiumBy similarity
    Metal bindingi181 – 1811MagnesiumBy similarity
    Metal bindingi346 – 3461MagnesiumBy similarity
    Metal bindingi353 – 3531Zinc 1By similarity
    Metal bindingi357 – 3571Zinc 1By similarity
    Metal bindingi395 – 3951Zinc 2By similarity
    Metal bindingi396 – 3961Zinc 2By similarity
    Metal bindingi438 – 4381Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase H (EC:3.1.3.1)
    Alternative name(s):
    High molecular weight phosphatase
    Short name:
    H-AP
    Gene namesi
    Name:phoA
    Ordered Locus Names:PA3296
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3296.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 476450Alkaline phosphatase HPRO_0000024014Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Expressed constitutively.

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA3296.

    Structurei

    3D structure databases

    ProteinModelPortaliP35483.
    SMRiP35483. Positions 33-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099117.
    KOiK01077.
    OMAiDKGFFLQ.
    OrthoDBiEOG661H4G.
    PhylomeDBiP35483.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 2 hits.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35483-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR    50
    VEQDLTQALK QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG 100
    IDALPLTGQY THYSLHKDSG LPDYVTDSAA SATAWSTGVK SYNGAIGVDI 150
    HEQPHRNLLE LAKLNGKATG NVSTAELQDA TPAALLAHVT ARKCYGPEAT 200
    SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET AKAGRYAGKT 250
    LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT 300
    YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA 350
    SIDKQDHAAN PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS 400
    QIIPPETAAP GLTQLLTTKD GAPLAISYGN SEESSQEHTG TQLRIAAYGP 450
    QAANVTGLTD QTDLFFTIRR ALNLRD 476
    Length:476
    Mass (Da):50,409
    Last modified:January 11, 2001 - v2
    Checksum:iA3059B30BBEA809C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271R → Q AA sequence (PubMed:8454193)Curated
    Sequence conflicti39 – 391R → A AA sequence (PubMed:8454193)Curated
    Sequence conflicti44 – 452EY → VR AA sequence (PubMed:8454193)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06684.1.
    PIRiA83235.
    RefSeqiNP_251986.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06684; AAG06684; PA3296.
    GeneIDi882459.
    KEGGipae:PA3296.
    PATRICi19841179. VBIPseAer58763_3448.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06684.1 .
    PIRi A83235.
    RefSeqi NP_251986.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali P35483.
    SMRi P35483. Positions 33-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06684 ; AAG06684 ; PA3296 .
    GeneIDi 882459.
    KEGGi pae:PA3296.
    PATRICi 19841179. VBIPseAer58763_3448.

    Organism-specific databases

    PseudoCAPi PA3296.

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099117.
    KOi K01077.
    OMAi DKGFFLQ.
    OrthoDBi EOG661H4G.
    PhylomeDBi P35483.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 2 hits.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. "Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
      Tan A.S.P., Worobec E.A.
      FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-45.
      Strain: H103.

    Entry informationi

    Entry nameiPPBH_PSEAE
    AccessioniPrimary (citable) accession number: P35483
    Secondary accession number(s): Q9HYU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3