Skip Header

Contribute Send feedback
Read comments (?) or add your own

P35483 (PPBH_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase H
EC=3.1.3.1
Alternative name(s):
High molecular weight phosphatase
Short name=H-AP
Gene names
Name:phoA
Ordered Locus Names:PA3296
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has only a phosphomonoesterase activity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion.

Binds 2 zinc ions.

Subcellular location

Secreted. Periplasm.

Developmental stage

Expressed constitutively.

Sequence similarities

Belongs to the alkaline phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
Secreted
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from electronic annotation. Source: GOC

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

periplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalkaline phosphatase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.2
Chain27 – 476450Alkaline phosphatase H
PRO_0000024014

Sites

Active site1281Phosphoserine intermediate By similarity
Metal binding771Magnesium By similarity
Metal binding771Zinc 2 By similarity
Metal binding1791Magnesium By similarity
Metal binding1811Magnesium By similarity
Metal binding3461Magnesium By similarity
Metal binding3531Zinc 1 By similarity
Metal binding3571Zinc 1 By similarity
Metal binding3951Zinc 2 By similarity
Metal binding3961Zinc 2 By similarity
Metal binding4381Zinc 1 By similarity

Experimental info

Sequence conflict271R → Q AA sequence Ref.2
Sequence conflict391R → A AA sequence Ref.2
Sequence conflict44 – 452EY → VR AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35483 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: A3059B30BBEA809C

FASTA47650,409
        10         20         30         40         50         60 
MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR VEQDLTQALK 

        70         80         90        100        110        120 
QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG IDALPLTGQY THYSLHKDSG 

       130        140        150        160        170        180 
LPDYVTDSAA SATAWSTGVK SYNGAIGVDI HEQPHRNLLE LAKLNGKATG NVSTAELQDA 

       190        200        210        220        230        240 
TPAALLAHVT ARKCYGPEAT SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET 

       250        260        270        280        290        300 
AKAGRYAGKT LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT 

       310        320        330        340        350        360 
YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA SIDKQDHAAN 

       370        380        390        400        410        420 
PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS QIIPPETAAP GLTQLLTTKD 

       430        440        450        460        470 
GAPLAISYGN SEESSQEHTG TQLRIAAYGP QAANVTGLTD QTDLFFTIRR ALNLRD

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
Tan A.S.P., Worobec E.A.
FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-45.
Strain: H103.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004091 Genomic DNA. Translation: AAG06684.1.
PIRA83235.
RefSeqNP_251986.1. NC_002516.2.

3D structure databases

ProteinModelPortalP35483.
SMRP35483. Positions 33-474.
ModBaseSearch...

Protein-protein interaction databases

STRING208964.PA3296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID882459.
KEGGpae:PA3296.
PATRIC19841179. VBIPseAer58763_3448.

Organism-specific databases

PseudoCAPPA3296.

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099117.
KOK01077.
OMACPSNALE.
ProtClustDBPRK10518.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPBH_PSEAE
AccessionPrimary (citable) accession number: P35483
Secondary accession number(s): Q9HYU7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents