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Protein

Alkaline phosphatase H

Gene

phoA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has only phosphomonoesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion per subunit.
  • Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77MagnesiumBy similarity1
Metal bindingi77Zinc 2By similarity1
Active sitei128Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi179MagnesiumBy similarity1
Metal bindingi181MagnesiumBy similarity1
Metal bindingi346MagnesiumBy similarity1
Metal bindingi353Zinc 1By similarity1
Metal bindingi357Zinc 1By similarity1
Metal bindingi395Zinc 2By similarity1
Metal bindingi396Zinc 2By similarity1
Metal bindingi438Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • protein secretion by the type II secretion system Source: PseudoCAP
  • protein transport by the Sec complex Source: PseudoCAP

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase H (EC:3.1.3.1)
Alternative name(s):
High molecular weight phosphatase1 Publication
Short name:
H-AP
Gene namesi
Name:phoA
Ordered Locus Names:PA3296
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3296.

Subcellular locationi

  • Secreted 1 Publication
  • Periplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000002401427 – 476Alkaline phosphatase HAdd BLAST450

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35483.

Expressioni

Developmental stagei

Expressed constitutively.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3296.

Structurei

3D structure databases

ProteinModelPortaliP35483.
SMRiP35483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099117.
InParanoidiP35483.
KOiK01077.
OMAiEHTGTQL.
PhylomeDBiP35483.

Family and domain databases

CDDicd16012. ALP. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
PANTHERiPTHR11596. PTHR11596. 1 hit.
PfamiView protein in Pfam
PF00245. Alk_phosphatase. 1 hit.
PRINTSiPR00113. ALKPHPHTASE.
SMARTiView protein in SMART
SM00098. alkPPc. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00123. ALKALINE_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR
60 70 80 90 100
VEQDLTQALK QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG
110 120 130 140 150
IDALPLTGQY THYSLHKDSG LPDYVTDSAA SATAWSTGVK SYNGAIGVDI
160 170 180 190 200
HEQPHRNLLE LAKLNGKATG NVSTAELQDA TPAALLAHVT ARKCYGPEAT
210 220 230 240 250
SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET AKAGRYAGKT
260 270 280 290 300
LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT
310 320 330 340 350
YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA
360 370 380 390 400
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS
410 420 430 440 450
QIIPPETAAP GLTQLLTTKD GAPLAISYGN SEESSQEHTG TQLRIAAYGP
460 470
QAANVTGLTD QTDLFFTIRR ALNLRD
Length:476
Mass (Da):50,409
Last modified:January 11, 2001 - v2
Checksum:iA3059B30BBEA809C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27R → Q AA sequence (PubMed:8454193).Curated1
Sequence conflicti39R → A AA sequence (PubMed:8454193).Curated1
Sequence conflicti44 – 45EY → VR AA sequence (PubMed:8454193).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06684.1.
PIRiA83235.
RefSeqiNP_251986.1. NC_002516.2.
WP_003113164.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06684; AAG06684; PA3296.
GeneIDi882459.
KEGGipae:PA3296.
PATRICifig|208964.12.peg.3448.

Similar proteinsi

Entry informationi

Entry nameiPPBH_PSEAE
AccessioniPrimary (citable) accession number: P35483
Secondary accession number(s): Q9HYU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: August 30, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families