Reviewed, UniProtKB/Swiss-Prot P35482 (PPBL_PSEAE)

Names and origin

Protein names

Recommended name:
    Alkaline phosphatase L
      Short name=L-AP
    EC=3.1.3.1
Alternative name(s):
    Low molecular weight phosphatase

Gene names

Name:	pstS
Ordered Locus Names:	PA0688

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has both a phosphomonoesterase and phosphodiesterase activity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit. Binds 2 zinc ions per subunit.
Subunit structure	Homodimer.
Subcellular location	Secreted. Periplasm. Note: Secreted and to some extent periplasmic.
Developmental stage	Expressed 7 hours after introduction into phosphate poor media.
Induction	In phosphate poor media.
Sequence similarities	Belongs to the pstS family.

Ontologies

Keywords
Cellular component	Periplasm Secreted
Domain	Signal
Ligand	Magnesium Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.3
	Chain	24 – 368	345	Alkaline phosphatase L		PRO_0000031860
Experimental info
	Sequence conflict	37	1	K → R Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35482-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 44F3D6BF203098F1	FASTA	368	37,885
	10 20 30 40 50 60 MFKRSLIAAS LSVAALVSAQ AMAVTGGGAS LPAELYKGSA DSILPANFSY AVTGSGTGKN 70 80 90 100 110 120 AFLTNNSSLF GTTGTVHYAG SDSVLSGSEL TTYNSNYNGT YGPLIQIPSV ATSVTVPYRK 130 140 150 160 170 180 DGNTTLNLTS AQLCDAFSGA KTTWGQLLGT TDSTPIRIVY RTGSSGTTEL FTRHLNSICP 190 200 210 220 230 240 TRFATNSTFT NARLPAGGTL PSNWVGVAAT STVVSTVKAT NGSLGYVSPD AVNINSNAEV 250 260 270 280 290 300 SRVNGNLPTQ ANVSTALGSV APPANAADRA DPSKWVPVFT NPSAGYSIVG YTNFVFGQCY 310 320 330 340 350 360 KDASVSTDVR AFINKHYGGT TTNAAVAAHG FIPLTPAWKS AIVSAFYTGT SENLAIGNTN VCNTKGRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and preliminary characterization of phoA encoding alkaline phosphatase of Pseudomonas aeruginosa." Schuermann M., Liebeton K., Jaeger K.-E. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa." Tan A.S.P., Worobec E.A. FEMS Microbiol. Lett. 106:281-286(1993) [PubMed: 8454193] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-37. Strain: H103.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047381 Genomic DNA. Translation: AAC04870.1. AE004091 Genomic DNA. Translation: AAG04077.1.
PIR	E83559.
RefSeq	NP_249379.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880790.
GenomeReviews	Gene locus PA0688 in contig AE004091_GR.
KEGG	pae:PA0688.
NMPDR	fig|208964.1.peg.689.
Organism-specific databases
PseudoCAP	PA0688.
CMR	Search...
Phylogenomic databases
HOGENOM	P35482.
OMA	PFNKAGS.
Enzyme and pathway databases
BioCyc	PAER208964:PA0688-MON.
BRENDA	3.1.3.1. 354.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PPBL_PSEAE

Accession

Primary (citable) accession number: P35482
Secondary accession number(s): O52768

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 11, 2001
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)