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P35482 (PPBL_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase L

Short name=L-AP
EC=3.1.3.1
Alternative name(s):
Low molecular weight phosphatase
Gene names
Name:pstS
Ordered Locus Names:PA0688
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has both a phosphomonoesterase and phosphodiesterase activity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit.

Binds 2 zinc ions per subunit.

Subunit structure

Homodimer.

Subcellular location

Secreted. Periplasm. Note: Secreted and to some extent periplasmic.

Developmental stage

Expressed 7 hours after introduction into phosphate poor media.

Induction

In phosphate poor media.

Sequence similarities

Belongs to the PstS family.

Ontologies

Keywords
   Cellular componentPeriplasm
Secreted
   DomainSignal
   LigandMagnesium
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

periplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalkaline phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.3
Chain24 – 368345Alkaline phosphatase L
PRO_0000031860

Experimental info

Sequence conflict371K → R Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35482 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 44F3D6BF203098F1

FASTA36837,885
        10         20         30         40         50         60 
MFKRSLIAAS LSVAALVSAQ AMAVTGGGAS LPAELYKGSA DSILPANFSY AVTGSGTGKN 

        70         80         90        100        110        120 
AFLTNNSSLF GTTGTVHYAG SDSVLSGSEL TTYNSNYNGT YGPLIQIPSV ATSVTVPYRK 

       130        140        150        160        170        180 
DGNTTLNLTS AQLCDAFSGA KTTWGQLLGT TDSTPIRIVY RTGSSGTTEL FTRHLNSICP 

       190        200        210        220        230        240 
TRFATNSTFT NARLPAGGTL PSNWVGVAAT STVVSTVKAT NGSLGYVSPD AVNINSNAEV 

       250        260        270        280        290        300 
SRVNGNLPTQ ANVSTALGSV APPANAADRA DPSKWVPVFT NPSAGYSIVG YTNFVFGQCY 

       310        320        330        340        350        360 
KDASVSTDVR AFINKHYGGT TTNAAVAAHG FIPLTPAWKS AIVSAFYTGT SENLAIGNTN 


VCNTKGRP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and preliminary characterization of phoA encoding alkaline phosphatase of Pseudomonas aeruginosa."
Schuermann M., Liebeton K., Jaeger K.-E.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
Tan A.S.P., Worobec E.A.
FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-37.
Strain: H103.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF047381 Genomic DNA. Translation: AAC04870.1.
AE004091 Genomic DNA. Translation: AAG04077.1.
PIRE83559.
RefSeqNP_249379.1. NC_002516.2.

3D structure databases

ProteinModelPortalP35482.
SMRP35482. Positions 24-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA0688.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG04077; AAG04077; PA0688.
GeneID880790.
KEGGpae:PA0688.
PATRIC19835651. VBIPseAer58763_0720.

Organism-specific databases

PseudoCAPPA0688.

Phylogenomic databases

eggNOGCOG0226.
OMAGRITYMS.
OrthoDBEOG6384GB.

Family and domain databases

InterProIPR024370. PBP_domain.
[Graphical view]
PfamPF12849. PBP_like_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPBL_PSEAE
AccessionPrimary (citable) accession number: P35482
Secondary accession number(s): O52768
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: June 11, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families