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P35482

- PPBL_PSEAE

UniProt

P35482 - PPBL_PSEAE

Protein

Alkaline phosphatase L

Gene

pstS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Has both a phosphomonoesterase and phosphodiesterase activity.

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.
    Binds 2 zinc ions per subunit.

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase L (EC:3.1.3.1)
    Short name:
    L-AP
    Alternative name(s):
    Low molecular weight phosphatase
    Gene namesi
    Name:pstS
    Ordered Locus Names:PA0688
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0688.

    Subcellular locationi

    Secreted. Periplasm
    Note: Secreted and to some extent periplasmic.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 368345Alkaline phosphatase LPRO_0000031860Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed 7 hours after introduction into phosphate poor media.

    Inductioni

    In phosphate poor media.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi208964.PA0688.

    Structurei

    3D structure databases

    ProteinModelPortaliP35482.
    SMRiP35482. Positions 24-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PstS family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0226.
    OMAiGRITYMS.
    OrthoDBiEOG6384GB.

    Family and domain databases

    InterProiIPR024370. PBP_domain.
    [Graphical view]
    PfamiPF12849. PBP_like_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35482-1 [UniParc]FASTAAdd to Basket

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    MFKRSLIAAS LSVAALVSAQ AMAVTGGGAS LPAELYKGSA DSILPANFSY    50
    AVTGSGTGKN AFLTNNSSLF GTTGTVHYAG SDSVLSGSEL TTYNSNYNGT 100
    YGPLIQIPSV ATSVTVPYRK DGNTTLNLTS AQLCDAFSGA KTTWGQLLGT 150
    TDSTPIRIVY RTGSSGTTEL FTRHLNSICP TRFATNSTFT NARLPAGGTL 200
    PSNWVGVAAT STVVSTVKAT NGSLGYVSPD AVNINSNAEV SRVNGNLPTQ 250
    ANVSTALGSV APPANAADRA DPSKWVPVFT NPSAGYSIVG YTNFVFGQCY 300
    KDASVSTDVR AFINKHYGGT TTNAAVAAHG FIPLTPAWKS AIVSAFYTGT 350
    SENLAIGNTN VCNTKGRP 368
    Length:368
    Mass (Da):37,885
    Last modified:January 11, 2001 - v2
    Checksum:i44F3D6BF203098F1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371K → R(PubMed:10984043)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF047381 Genomic DNA. Translation: AAC04870.1.
    AE004091 Genomic DNA. Translation: AAG04077.1.
    PIRiE83559.
    RefSeqiNP_249379.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG04077; AAG04077; PA0688.
    GeneIDi880790.
    KEGGipae:PA0688.
    PATRICi19835651. VBIPseAer58763_0720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF047381 Genomic DNA. Translation: AAC04870.1 .
    AE004091 Genomic DNA. Translation: AAG04077.1 .
    PIRi E83559.
    RefSeqi NP_249379.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali P35482.
    SMRi P35482. Positions 24-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA0688.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG04077 ; AAG04077 ; PA0688 .
    GeneIDi 880790.
    KEGGi pae:PA0688.
    PATRICi 19835651. VBIPseAer58763_0720.

    Organism-specific databases

    PseudoCAPi PA0688.

    Phylogenomic databases

    eggNOGi COG0226.
    OMAi GRITYMS.
    OrthoDBi EOG6384GB.

    Family and domain databases

    InterProi IPR024370. PBP_domain.
    [Graphical view ]
    Pfami PF12849. PBP_like_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and preliminary characterization of phoA encoding alkaline phosphatase of Pseudomonas aeruginosa."
      Schuermann M., Liebeton K., Jaeger K.-E.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    3. "Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
      Tan A.S.P., Worobec E.A.
      FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-37.
      Strain: H103.

    Entry informationi

    Entry nameiPPBL_PSEAE
    AccessioniPrimary (citable) accession number: P35482
    Secondary accession number(s): O52768
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3