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Protein

Alkaline phosphatase L

Gene

phoA2

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has both a phosphomonoesterase and phosphodiesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase L1 Publication (EC:3.1.3.1)
Short name:
L-AP
Alternative name(s):
Low molecular weight phosphatase
Protein DING1 Publication
Gene namesi
Name:phoA2Curated
Synonyms:phoA1 Publication, pstS
Ordered Locus Names:PA0688
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0688.

Subcellular locationi

  • Secreted 1 Publication
  • Periplasm 1 Publication

  • Note: Secreted and to some extent periplasmic.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 368345Alkaline phosphatase LPRO_0000031860Add
BLAST

Expressioni

Developmental stagei

Expressed 7 hours after introduction into phosphate poor media.1 Publication

Inductioni

Contradictory; shown to be induced in phosphate poor media (PubMed:8454193). Does not respond to decreased phosphate (PubMed:18282104).2 Publications

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi208964.PA0688.

Structurei

3D structure databases

ProteinModelPortaliP35482.
SMRiP35482. Positions 24-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PstS family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0226.
InParanoidiP35482.
OMAiGRITYMS.
OrthoDBiEOG6384GB.

Family and domain databases

InterProiIPR024370. PBP_domain.
[Graphical view]
PfamiPF12849. PBP_like_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRSLIAAS LSVAALVSAQ AMAVTGGGAS LPAELYKGSA DSILPANFSY
60 70 80 90 100
AVTGSGTGKN AFLTNNSSLF GTTGTVHYAG SDSVLSGSEL TTYNSNYNGT
110 120 130 140 150
YGPLIQIPSV ATSVTVPYRK DGNTTLNLTS AQLCDAFSGA KTTWGQLLGT
160 170 180 190 200
TDSTPIRIVY RTGSSGTTEL FTRHLNSICP TRFATNSTFT NARLPAGGTL
210 220 230 240 250
PSNWVGVAAT STVVSTVKAT NGSLGYVSPD AVNINSNAEV SRVNGNLPTQ
260 270 280 290 300
ANVSTALGSV APPANAADRA DPSKWVPVFT NPSAGYSIVG YTNFVFGQCY
310 320 330 340 350
KDASVSTDVR AFINKHYGGT TTNAAVAAHG FIPLTPAWKS AIVSAFYTGT
360
SENLAIGNTN VCNTKGRP
Length:368
Mass (Da):37,885
Last modified:January 11, 2001 - v2
Checksum:i44F3D6BF203098F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371K → R (PubMed:10984043).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047381 Genomic DNA. Translation: AAC04870.1.
AE004091 Genomic DNA. Translation: AAG04077.1.
PIRiE83559.
RefSeqiNP_249379.1. NC_002516.2.
WP_003112123.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04077; AAG04077; PA0688.
GeneIDi880790.
KEGGipae:PA0688.
PATRICi19835651. VBIPseAer58763_0720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047381 Genomic DNA. Translation: AAC04870.1.
AE004091 Genomic DNA. Translation: AAG04077.1.
PIRiE83559.
RefSeqiNP_249379.1. NC_002516.2.
WP_003112123.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP35482.
SMRiP35482. Positions 24-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04077; AAG04077; PA0688.
GeneIDi880790.
KEGGipae:PA0688.
PATRICi19835651. VBIPseAer58763_0720.

Organism-specific databases

PseudoCAPiPA0688.

Phylogenomic databases

eggNOGiCOG0226.
InParanoidiP35482.
OMAiGRITYMS.
OrthoDBiEOG6384GB.

Family and domain databases

InterProiIPR024370. PBP_domain.
[Graphical view]
PfamiPF12849. PBP_like_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and preliminary characterization of phoA encoding alkaline phosphatase of Pseudomonas aeruginosa."
    Schuermann M., Liebeton K., Jaeger K.-E.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa."
    Tan A.S.P., Worobec E.A.
    FEMS Microbiol. Lett. 106:281-286(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-37, FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
    Strain: H103.
  4. "Structure-function aspects of PstS in multi-drug-resistant Pseudomonas aeruginosa."
    Zaborina O., Holbrook C., Chen Y., Long J., Zaborin A., Morozova I., Fernandez H., Wang Y., Turner J.R., Alverdy J.C.
    PLoS Pathog. 4:E43-E43(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiPPBL_PSEAE
AccessioniPrimary (citable) accession number: P35482
Secondary accession number(s): O52768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: May 27, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.