Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alkaline phosphatase L

Gene

phoA2

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both a phosphomonoesterase and phosphodiesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

GO - Molecular functioni

GO - Biological processi

  • protein secretion by the type II secretion system Source: PseudoCAP
  • protein transport by the Sec complex Source: PseudoCAP

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase L1 Publication (EC:3.1.3.1)
Short name:
L-AP
Alternative name(s):
Low molecular weight phosphatase
Protein DING1 Publication
Gene namesi
Name:phoA2Curated
Synonyms:phoA1 Publication, pstS
Ordered Locus Names:PA0688
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0688.

Subcellular locationi

  • Secreted 1 Publication
  • Periplasm 1 Publication

  • Note: Secreted and to some extent periplasmic.1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000003186024 – 368Alkaline phosphatase LAdd BLAST345

Proteomic databases

PaxDbiP35482.

Expressioni

Developmental stagei

Expressed 7 hours after introduction into phosphate poor media.1 Publication

Inductioni

Contradictory; shown to be induced in phosphate poor media (PubMed:8454193). Does not respond to decreased phosphate (PubMed:18282104).2 Publications

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi208964.PA0688.

Structurei

3D structure databases

ProteinModelPortaliP35482.
SMRiP35482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PstS family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108UPW. Bacteria.
COG0226. LUCA.
InParanoidiP35482.
OMAiKGPKNDG.

Family and domain databases

InterProiView protein in InterPro
IPR024370. PBP_domain.
PfamiView protein in Pfam
PF12849. PBP_like_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRSLIAAS LSVAALVSAQ AMAVTGGGAS LPAELYKGSA DSILPANFSY
60 70 80 90 100
AVTGSGTGKN AFLTNNSSLF GTTGTVHYAG SDSVLSGSEL TTYNSNYNGT
110 120 130 140 150
YGPLIQIPSV ATSVTVPYRK DGNTTLNLTS AQLCDAFSGA KTTWGQLLGT
160 170 180 190 200
TDSTPIRIVY RTGSSGTTEL FTRHLNSICP TRFATNSTFT NARLPAGGTL
210 220 230 240 250
PSNWVGVAAT STVVSTVKAT NGSLGYVSPD AVNINSNAEV SRVNGNLPTQ
260 270 280 290 300
ANVSTALGSV APPANAADRA DPSKWVPVFT NPSAGYSIVG YTNFVFGQCY
310 320 330 340 350
KDASVSTDVR AFINKHYGGT TTNAAVAAHG FIPLTPAWKS AIVSAFYTGT
360
SENLAIGNTN VCNTKGRP
Length:368
Mass (Da):37,885
Last modified:January 11, 2001 - v2
Checksum:i44F3D6BF203098F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37K → R (PubMed:10984043).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047381 Genomic DNA. Translation: AAC04870.1.
AE004091 Genomic DNA. Translation: AAG04077.1.
PIRiE83559.
RefSeqiNP_249379.1. NC_002516.2.
WP_003112123.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04077; AAG04077; PA0688.
GeneIDi880790.
KEGGipae:PA0688.
PATRICifig|208964.12.peg.720.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPPBL_PSEAE
AccessioniPrimary (citable) accession number: P35482
Secondary accession number(s): O52768
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2001
Last modified: July 5, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families