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Protein

Protein S100-A1

Gene

S100a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi20 – 33141; low affinityAdd
BLAST
Calcium bindingi63 – 74122; high affinityAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent protein binding Source: RGD
  2. calcium ion binding Source: RGD
  3. protein homodimerization activity Source: RGD

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: RGD
  2. positive regulation of voltage-gated calcium channel activity Source: RGD
  3. regulation of heart contraction Source: InterPro
  4. substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-A1
Alternative name(s):
S-100 protein alpha chain
S-100 protein subunit alpha
S100 calcium-binding protein A1
Gene namesi
Name:S100a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3614. S100a1.

Subcellular locationi

GO - Cellular componenti

  1. A band Source: RGD
  2. cytoplasm Source: RGD
  3. cytosol Source: RGD
  4. I band Source: RGD
  5. M band Source: RGD
  6. neuron projection Source: RGD
  7. nucleus Source: Ensembl
  8. sarcoplasmic reticulum Source: Ensembl
  9. Z disc Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 9493Protein S100-A1PRO_0000143963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861S-nitrosocysteineBy similarity

Keywords - PTMi

S-nitrosylation

Proteomic databases

PaxDbiP35467.
PRIDEiP35467.

Expressioni

Tissue specificityi

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Gene expression databases

GenevestigatoriP35467.

Interactioni

Subunit structurei

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Also forms heterodimers with S100P. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Interacts with CACYBP in a calcium-dependent manner. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FKBP4Q027907EBI-6477109,EBI-1047444From a different organism.
HSP90AA1P079004EBI-6477109,EBI-296047From a different organism.
HSPA1BP081074EBI-6477109,EBI-629985From a different organism.
PPIDP268827EBI-6477109,EBI-6477155From a different organism.

Protein-protein interaction databases

IntActiP35467. 4 interactions.
MINTiMINT-4591010.
STRINGi10116.ENSRNOP00000017000.

Structurei

Secondary structure

1
94
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2017Combined sources
Beta strandi22 – 254Combined sources
Beta strandi27 – 304Combined sources
Helixi31 – 4111Combined sources
Helixi43 – 475Combined sources
Helixi49 – 513Combined sources
Helixi52 – 6413Combined sources
Beta strandi68 – 703Combined sources
Helixi72 – 8514Combined sources
Helixi91 – 933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K2HNMR-A/B2-94[»]
1ZFSNMR-A/B2-94[»]
2K2FNMR-A/B2-94[»]
2KBMNMR-A/B2-94[»]
SMRiP35467. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35467.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the S-100 family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG277258.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP35467.
OMAiINVFHTH.
OrthoDBiEOG7R833W.
PhylomeDBiP35467.
TreeFamiTF332727.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028486. S100-A1.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF66. PTHR11639:SF66. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSSFLDVQK
60 70 80 90
DADAVDKIMK ELDENGDGEV DFQEFVVLVA ALTVACNNFF WENS
Length:94
Mass (Da):10,560
Last modified:January 23, 2007 - v3
Checksum:i7B9C0A6C1C4FCCE0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141N → H in AAB20539 (PubMed:1742602).Curated
Sequence conflicti57 – 571K → R in AAB20539 (PubMed:1742602).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26358, U26357 Genomic DNA. Translation: AAB53657.1.
S68809 mRNA. Translation: AAB20539.2.
RefSeqiNP_001007637.1. NM_001007636.2.
XP_006232665.1. XM_006232603.2.
UniGeneiRn.11091.

Genome annotation databases

EnsembliENSRNOT00000017000; ENSRNOP00000017000; ENSRNOG00000012410.
GeneIDi295214.
KEGGirno:295214.
UCSCiRGD:3614. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26358, U26357 Genomic DNA. Translation: AAB53657.1.
S68809 mRNA. Translation: AAB20539.2.
RefSeqiNP_001007637.1. NM_001007636.2.
XP_006232665.1. XM_006232603.2.
UniGeneiRn.11091.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K2HNMR-A/B2-94[»]
1ZFSNMR-A/B2-94[»]
2K2FNMR-A/B2-94[»]
2KBMNMR-A/B2-94[»]
SMRiP35467. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP35467. 4 interactions.
MINTiMINT-4591010.
STRINGi10116.ENSRNOP00000017000.

Proteomic databases

PaxDbiP35467.
PRIDEiP35467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017000; ENSRNOP00000017000; ENSRNOG00000012410.
GeneIDi295214.
KEGGirno:295214.
UCSCiRGD:3614. rat.

Organism-specific databases

CTDi6271.
RGDi3614. S100a1.

Phylogenomic databases

eggNOGiNOG277258.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP35467.
OMAiINVFHTH.
OrthoDBiEOG7R833W.
PhylomeDBiP35467.
TreeFamiTF332727.

Miscellaneous databases

EvolutionaryTraceiP35467.
NextBioi639119.
PROiP35467.

Gene expression databases

GenevestigatoriP35467.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028486. S100-A1.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF66. PTHR11639:SF66. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Song W.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  2. "Isolation of a rat S100 alpha cDNA and distribution of its mRNA in rat tissues."
    Zimmer D.B., Song W., Zimmer W.E.
    Brain Res. Bull. 27:157-162(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-94.
    Tissue: Kidney.
  3. "S100B interaction with the receptor for advanced glycation end products (RAGE): a novel receptor-mediated mechanism for myocyte apoptosis postinfarction."
    Tsoporis J.N., Izhar S., Leong-Poi H., Desjardins J.F., Huttunen H.J., Parker T.G.
    Circ. Res. 106:93-101(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AGER.
  4. "Three-dimensional solution structure of the calcium-signaling protein apo-S100A1 as determined by NMR."
    Rustandi R.R., Baldisseri D.M., Inman K.G., Nizner P., Hamilton S.M., Landar A., Landar A., Zimmer D.B., Weber D.J.
    Biochemistry 41:788-796(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  5. Cited for: STRUCTURE BY NMR IN COMPLEX WITH CALCIUM IONS AND CAPZA1, SUBUNIT.
  6. "S100A1 and calmodulin compete for the same binding site on ryanodine receptor."
    Wright N.T., Prosser B.L., Varney K.M., Zimmer D.B., Schneider M.F., Weber D.J.
    J. Biol. Chem. 283:26676-26683(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR IN COMPLEX WITH RYR1, INTERACTION WITH RYR1 AND RYR2.

Entry informationi

Entry nameiS10A1_RAT
AccessioniPrimary (citable) accession number: P35467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.