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P35467

- S10A1_RAT

UniProt

P35467 - S10A1_RAT

Protein

Protein S100-A1

Gene

S100a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi20 – 33141; low affinityAdd
    BLAST
    Calcium bindingi63 – 74122; high affinityAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: RGD
    2. calcium ion binding Source: RGD
    3. protein binding Source: UniProtKB
    4. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: RGD
    2. positive regulation of voltage-gated calcium channel activity Source: RGD
    3. regulation of heart contraction Source: InterPro

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein S100-A1
    Alternative name(s):
    S-100 protein alpha chain
    S-100 protein subunit alpha
    S100 calcium-binding protein A1
    Gene namesi
    Name:S100a1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi3614. S100a1.

    Subcellular locationi

    GO - Cellular componenti

    1. A band Source: RGD
    2. cytoplasm Source: RGD
    3. cytosol Source: RGD
    4. I band Source: RGD
    5. M band Source: RGD
    6. neuron projection Source: RGD
    7. nucleus Source: Ensembl
    8. sarcoplasmic reticulum Source: Ensembl
    9. Z disc Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 9493Protein S100-A1PRO_0000143963Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei86 – 861S-nitrosocysteineBy similarity

    Keywords - PTMi

    S-nitrosylation

    Proteomic databases

    PaxDbiP35467.
    PRIDEiP35467.

    Expressioni

    Tissue specificityi

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

    Gene expression databases

    GenevestigatoriP35467.

    Interactioni

    Subunit structurei

    Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Also forms heterodimers with S100P. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Interacts with CACYBP in a calcium-dependent manner. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FKBP4Q027907EBI-6477109,EBI-1047444From a different organism.
    HSP90AA1P079004EBI-6477109,EBI-296047From a different organism.
    HSPA1BP081074EBI-6477109,EBI-629985From a different organism.
    PPIDP268827EBI-6477109,EBI-6477155From a different organism.

    Protein-protein interaction databases

    IntActiP35467. 4 interactions.
    MINTiMINT-4591010.
    STRINGi10116.ENSRNOP00000017000.

    Structurei

    Secondary structure

    1
    94
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2017
    Beta strandi22 – 254
    Beta strandi27 – 304
    Helixi31 – 4111
    Helixi43 – 475
    Helixi49 – 513
    Helixi52 – 6413
    Beta strandi68 – 703
    Helixi72 – 8514
    Helixi91 – 933

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K2HNMR-A/B2-94[»]
    1ZFSNMR-A/B2-94[»]
    2K2FNMR-A/B2-94[»]
    2KBMNMR-A/B2-94[»]
    ProteinModelPortaliP35467.
    SMRiP35467. Positions 2-94.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35467.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the S-100 family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG277258.
    GeneTreeiENSGT00740000114954.
    HOGENOMiHOG000246968.
    HOVERGENiHBG001479.
    InParanoidiP35467.
    OMAiINVFHTH.
    OrthoDBiEOG7R833W.
    PhylomeDBiP35467.
    TreeFamiTF332727.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028486. S100-A1.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view]
    PANTHERiPTHR11639:SF66. PTHR11639:SF66. 1 hit.
    PfamiPF00036. EF-hand_1. 1 hit.
    PF01023. S_100. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35467-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSSFLDVQK   50
    DADAVDKIMK ELDENGDGEV DFQEFVVLVA ALTVACNNFF WENS 94
    Length:94
    Mass (Da):10,560
    Last modified:January 23, 2007 - v3
    Checksum:i7B9C0A6C1C4FCCE0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141N → H in AAB20539. (PubMed:1742602)Curated
    Sequence conflicti57 – 571K → R in AAB20539. (PubMed:1742602)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26358, U26357 Genomic DNA. Translation: AAB53657.1.
    S68809 mRNA. Translation: AAB20539.2.
    RefSeqiNP_001007637.1. NM_001007636.2.
    XP_006232665.1. XM_006232603.1.
    UniGeneiRn.11091.

    Genome annotation databases

    EnsembliENSRNOT00000017000; ENSRNOP00000017000; ENSRNOG00000012410.
    GeneIDi295214.
    KEGGirno:295214.
    UCSCiRGD:3614. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26358 , U26357 Genomic DNA. Translation: AAB53657.1 .
    S68809 mRNA. Translation: AAB20539.2 .
    RefSeqi NP_001007637.1. NM_001007636.2.
    XP_006232665.1. XM_006232603.1.
    UniGenei Rn.11091.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K2H NMR - A/B 2-94 [» ]
    1ZFS NMR - A/B 2-94 [» ]
    2K2F NMR - A/B 2-94 [» ]
    2KBM NMR - A/B 2-94 [» ]
    ProteinModelPortali P35467.
    SMRi P35467. Positions 2-94.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35467. 4 interactions.
    MINTi MINT-4591010.
    STRINGi 10116.ENSRNOP00000017000.

    Proteomic databases

    PaxDbi P35467.
    PRIDEi P35467.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000017000 ; ENSRNOP00000017000 ; ENSRNOG00000012410 .
    GeneIDi 295214.
    KEGGi rno:295214.
    UCSCi RGD:3614. rat.

    Organism-specific databases

    CTDi 6271.
    RGDi 3614. S100a1.

    Phylogenomic databases

    eggNOGi NOG277258.
    GeneTreei ENSGT00740000114954.
    HOGENOMi HOG000246968.
    HOVERGENi HBG001479.
    InParanoidi P35467.
    OMAi INVFHTH.
    OrthoDBi EOG7R833W.
    PhylomeDBi P35467.
    TreeFami TF332727.

    Miscellaneous databases

    EvolutionaryTracei P35467.
    NextBioi 639119.
    PROi P35467.

    Gene expression databases

    Genevestigatori P35467.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028486. S100-A1.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view ]
    PANTHERi PTHR11639:SF66. PTHR11639:SF66. 1 hit.
    Pfami PF00036. EF-hand_1. 1 hit.
    PF01023. S_100. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Song W.
      Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    2. "Isolation of a rat S100 alpha cDNA and distribution of its mRNA in rat tissues."
      Zimmer D.B., Song W., Zimmer W.E.
      Brain Res. Bull. 27:157-162(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-94.
      Tissue: Kidney.
    3. "S100B interaction with the receptor for advanced glycation end products (RAGE): a novel receptor-mediated mechanism for myocyte apoptosis postinfarction."
      Tsoporis J.N., Izhar S., Leong-Poi H., Desjardins J.F., Huttunen H.J., Parker T.G.
      Circ. Res. 106:93-101(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AGER.
    4. "Three-dimensional solution structure of the calcium-signaling protein apo-S100A1 as determined by NMR."
      Rustandi R.R., Baldisseri D.M., Inman K.G., Nizner P., Hamilton S.M., Landar A., Landar A., Zimmer D.B., Weber D.J.
      Biochemistry 41:788-796(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    5. Cited for: STRUCTURE BY NMR IN COMPLEX WITH CALCIUM IONS AND CAPZA1, SUBUNIT.
    6. "S100A1 and calmodulin compete for the same binding site on ryanodine receptor."
      Wright N.T., Prosser B.L., Varney K.M., Zimmer D.B., Schneider M.F., Weber D.J.
      J. Biol. Chem. 283:26676-26683(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR IN COMPLEX WITH RYR1, INTERACTION WITH RYR1 AND RYR2.

    Entry informationi

    Entry nameiS10A1_RAT
    AccessioniPrimary (citable) accession number: P35467
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3