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Protein

Protein S100-A1

Gene

S100a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably acts as a Ca2+ signal transducer (By similarity). In response to an increase in intracellular Ca2+ levels, binds calcium which triggers a conformational change (PubMed:16169012, PubMed:18650434). This conformational change allows interaction of S1001A with specific target proteins, such as TPR-containing proteins, and the modulation of their activity (By similarity).By similarity2 Publications

Miscellaneous

Able to bind zinc in vitro; the binding sites are different from the calcium binding sites. The physiological relevance of zinc binding is unclear. Physiological concentrations of potassium antagonize the binding of both divalent cations, especially affecting the high-affinity calcium-binding sites.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi20 – 331; low affinity3 PublicationsAdd BLAST14
Calcium bindingi63 – 742; high affinity3 PublicationsAdd BLAST12

GO - Molecular functioni

  • ATPase binding Source: Ensembl
  • calcium-dependent protein binding Source: RGD
  • calcium ion binding Source: RGD
  • protein homodimerization activity Source: RGD
  • S100 protein binding Source: Ensembl

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-A1
Alternative name(s):
S-100 protein alpha chain
S-100 protein subunit alpha
S100 calcium-binding protein A1
Gene namesi
Name:S100a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3614. S100a1.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • A band Source: RGD
  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • I band Source: RGD
  • M band Source: RGD
  • neuron projection Source: RGD
  • nucleus Source: Ensembl
  • sarcoplasmic reticulum Source: Ensembl
  • Z disc Source: RGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001439631 – 94Protein S100-A1Add BLAST94

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86S-nitrosocysteineBy similarity1

Keywords - PTMi

S-nitrosylation

Proteomic databases

PaxDbiP35467.
PRIDEiP35467.

Expressioni

Tissue specificityi

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.1 Publication

Gene expression databases

BgeeiENSRNOG00000012410.
GenevisibleiP35467. RN.

Interactioni

Subunit structurei

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (By similarity). Also forms heterodimers with S100P (By similarity). Interacts with AGER (PubMed:19910580). Interacts with CAPZA1 (PubMed:19452629). Interacts with FKBP4. Interacts with RYR1 and RYR2 (PubMed:18650434). Interacts with CACYBP in a calcium-dependent manner (By similarity). Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ATPase binding Source: Ensembl
  • calcium-dependent protein binding Source: RGD
  • protein homodimerization activity Source: RGD
  • S100 protein binding Source: Ensembl

Protein-protein interaction databases

BioGridi254914. 1 interactor.
IntActiP35467. 4 interactors.
MINTiMINT-4591010.
STRINGi10116.ENSRNOP00000017000.

Structurei

Secondary structure

194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 20Combined sources17
Beta strandi22 – 25Combined sources4
Beta strandi27 – 30Combined sources4
Helixi31 – 41Combined sources11
Helixi43 – 47Combined sources5
Helixi49 – 51Combined sources3
Helixi52 – 64Combined sources13
Beta strandi68 – 70Combined sources3
Helixi72 – 85Combined sources14
Helixi91 – 93Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K2HNMR-A/B2-94[»]
1ZFSNMR-A/B2-94[»]
2K2FNMR-A/B2-94[»]
2KBMNMR-A/B2-94[»]
ProteinModelPortaliP35467.
SMRiP35467.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35467.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 48EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini50 – 85EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the S-100 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IXZ2. Eukaryota.
ENOG4111987. LUCA.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP35467.
OMAiTAAMGSE.
OrthoDBiEOG091G10JX.
PhylomeDBiP35467.
TreeFamiTF332727.

Family and domain databases

CDDicd05025. S-100A1. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028486. S100-A1.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
PANTHERiPTHR11639:SF86. PTHR11639:SF86. 1 hit.
PfamiView protein in Pfam
PF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 1 hit.
SM01394. S_100. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.

Sequencei

Sequence statusi: Complete.

P35467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSSFLDVQK
60 70 80 90
DADAVDKIMK ELDENGDGEV DFQEFVVLVA ALTVACNNFF WENS
Length:94
Mass (Da):10,560
Last modified:January 23, 2007 - v3
Checksum:i7B9C0A6C1C4FCCE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14N → H in AAB20539 (PubMed:1742602).Curated1
Sequence conflicti57K → R in AAB20539 (PubMed:1742602).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26358, U26357 Genomic DNA. Translation: AAB53657.1.
S68809 mRNA. Translation: AAB20539.2.
RefSeqiNP_001007637.1. NM_001007636.3.

Genome annotation databases

EnsembliENSRNOT00000017000; ENSRNOP00000017000; ENSRNOG00000012410.
GeneIDi295214.
KEGGirno:295214.
UCSCiRGD:3614. rat.

Similar proteinsi

Entry informationi

Entry nameiS10A1_RAT
AccessioniPrimary (citable) accession number: P35467
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families