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P35456

- UPAR_MOUSE

UniProt

P35456 - UPAR_MOUSE

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Protein

Urokinase plasminogen activator surface receptor

Gene

Plaur

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase plasminogen activator surface receptor
Short name:
U-PAR
Short name:
uPAR
Alternative name(s):
CD_antigen: CD87
Gene namesi
Name:Plaur
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:97612. Plaur.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – ?298275Urokinase plasminogen activator surface receptorPRO_0000036096Add
BLAST
Propeptidei?299 – 32729Removed in mature formSequence AnalysisPRO_0000036097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 471 Publication
Disulfide bondi29 ↔ 351 Publication
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi40 ↔ 681 Publication
Glycosylationi75 – 751N-linked (GlcNAc...)1 Publication
Disulfide bondi94 ↔ 991 Publication
Disulfide bondi119 ↔ 1461 Publication
Disulfide bondi122 ↔ 1291 Publication
Disulfide bondi139 ↔ 1681 Publication
Disulfide bondi174 ↔ 1911 Publication
Glycosylationi183 – 1831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi192 ↔ 1971 Publication
Glycosylationi193 – 1931N-linked (GlcNAc...)1 Publication
Disulfide bondi215 ↔ 2431 Publication
Disulfide bondi218 ↔ 2261 Publication
Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi236 ↔ 2621 Publication
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi268 ↔ 2871 Publication
Glycosylationi282 – 2821N-linked (GlcNAc...)1 Publication
Disulfide bondi288 ↔ 2931 Publication
Lipidationi298 – 2981GPI-anchor amidated glycineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP35456.
PaxDbiP35456.
PRIDEiP35456.

Miscellaneous databases

PMAP-CutDBP35456.

Expressioni

Tissue specificityi

Expressed in angiogenic endothelial cells (at protein level).1 Publication

Gene expression databases

BgeeiP35456.
CleanExiMM_PLAUR.
ExpressionAtlasiP35456. baseline and differential.
GenevestigatoriP35456.

Interactioni

Subunit structurei

Monomer (Probable). Interacts (via the UPAR/Ly6 domains) with SRPX2. Interacts with MRC2 (By similarity). Interacts with SORL1 (By similarity).By similarityCurated

Protein-protein interaction databases

IntActiP35456. 1 interaction.

Structurei

Secondary structure

1
327
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 295
Beta strandi36 – 394
Beta strandi47 – 5610
Beta strandi61 – 688
Beta strandi76 – 827
Beta strandi85 – 917
Beta strandi118 – 1203
Turni124 – 1274
Beta strandi129 – 1324
Beta strandi136 – 1383
Beta strandi146 – 1538
Beta strandi163 – 1708
Beta strandi175 – 1817
Beta strandi183 – 19412
Turni195 – 1984
Beta strandi214 – 2185
Beta strandi220 – 23011
Beta strandi232 – 2354
Beta strandi242 – 2465
Beta strandi259 – 2635
Beta strandi265 – 2717
Helixi272 – 2754
Beta strandi285 – 2917
Helixi292 – 2943

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LAQX-ray3.20U/V24-300[»]
ProteinModelPortaliP35456.
SMRiP35456. Positions 24-297.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35456.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 11794UPAR/Ly6 1Add
BLAST
Domaini117 – 21296UPAR/Ly6 2Add
BLAST
Domaini213 – 29886UPAR/Ly6 3Add
BLAST

Sequence similaritiesi

Contains 3 UPAR/Ly6 domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG41870.
HOGENOMiHOG000136855.
HOVERGENiHBG000245.
InParanoidiP35456.
KOiK03985.
OMAiTNRTMSY.
OrthoDBiEOG7QRQVG.
PhylomeDBiP35456.
TreeFamiTF338662.

Family and domain databases

InterProiIPR018363. CD59_antigen_CS.
IPR016054. LY6_UPA_recep-like.
IPR001526. LY6_UPAR.
[Graphical view]
PfamiPF00021. UPAR_LY6. 3 hits.
[Graphical view]
SMARTiSM00134. LU. 3 hits.
[Graphical view]
PROSITEiPS00983. LY6_UPAR. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P35456-1) [UniParc]FASTAAdd to Basket

Also known as: GPI-anchored

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLPRRLLLL LLLATTCVPA SQGLQCMQCE SNQSCLVEEC ALGQDLCRTT
60 70 80 90 100
VLREWQDDRE LEVVTRGCAH SEKTNRTMSY RMGSMIISLT ETVCATNLCN
110 120 130 140 150
RPRPGARGRA FPQGRYLECA SCTSLDQSCE RGREQSLQCR YPTEHCIEVV
160 170 180 190 200
TLQSTERSLK DEDYTRGCGS LPGCPGTAGF HSNQTFHFLK CCNYTHCNGG
210 220 230 240 250
PVLDLQSFPP NGFQCYSCEG NNTLGCSSEE ASLINCRGPM NQCLVATGLD
260 270 280 290 300
VLGNRSYTVR GCATASWCQG SHVADSFPTH LNVSVSCCHG SGCNSPTGGA
310 320
PRPGPAQLSL IASLLLTLGL WGVLLWT

Note: GPI-anchored form.

Length:327
Mass (Da):35,428
Last modified:June 1, 1994 - v1
Checksum:iA117441D738B6343
GO
Isoform 2 (identifier: P35456-2) [UniParc] [UniParc]FASTAAdd to Basket

Also known as: Secreted

The sequence of this isoform differs from the canonical sequence as follows:
     157-222: RSLKDEDYTR...FQCYSCEGNN → SKLPSAGQLL...LVMSRILLSF
     223-327: Missing.

Show »
Length:222
Mass (Da):24,619
Checksum:i8BA6B9F21389C587
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei157 – 22266RSLKD…CEGNN → SKLPSAGQLLVEIFKSWEQS ASKRQLNPHTVTGPTFSVTG SSGSLDQLGSDQEPSYLVMS RILLSF in isoform 2. 1 PublicationVSP_031837Add
BLAST
Alternative sequencei223 – 327105Missing in isoform 2. 1 PublicationVSP_031838Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62700 mRNA. Translation: CAA44574.1.
X62701 mRNA. Translation: CAA44575.1.
U12235 Genomic DNA. Translation: AAB60484.1.
CCDSiCCDS20950.1. [P35456-1]
PIRiA55356.
B41643.
RefSeqiNP_035243.1. NM_011113.3. [P35456-1]
XP_006539702.1. XM_006539639.1. [P35456-1]
XP_006539703.1. XM_006539640.1. [P35456-1]
XP_006539704.1. XM_006539641.1. [P35456-1]
UniGeneiMm.1359.

Genome annotation databases

EnsembliENSMUST00000002284; ENSMUSP00000002284; ENSMUSG00000046223. [P35456-1]
GeneIDi18793.
KEGGimmu:18793.
UCSCiuc009fpr.1. mouse. [P35456-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62700 mRNA. Translation: CAA44574.1 .
X62701 mRNA. Translation: CAA44575.1 .
U12235 Genomic DNA. Translation: AAB60484.1 .
CCDSi CCDS20950.1. [P35456-1 ]
PIRi A55356.
B41643.
RefSeqi NP_035243.1. NM_011113.3. [P35456-1 ]
XP_006539702.1. XM_006539639.1. [P35456-1 ]
XP_006539703.1. XM_006539640.1. [P35456-1 ]
XP_006539704.1. XM_006539641.1. [P35456-1 ]
UniGenei Mm.1359.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LAQ X-ray 3.20 U/V 24-300 [» ]
ProteinModelPortali P35456.
SMRi P35456. Positions 24-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35456. 1 interaction.

Proteomic databases

MaxQBi P35456.
PaxDbi P35456.
PRIDEi P35456.

Protocols and materials databases

DNASUi 18793.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002284 ; ENSMUSP00000002284 ; ENSMUSG00000046223 . [P35456-1 ]
GeneIDi 18793.
KEGGi mmu:18793.
UCSCi uc009fpr.1. mouse. [P35456-1 ]

Organism-specific databases

CTDi 5329.
MGIi MGI:97612. Plaur.

Phylogenomic databases

eggNOGi NOG41870.
HOGENOMi HOG000136855.
HOVERGENi HBG000245.
InParanoidi P35456.
KOi K03985.
OMAi TNRTMSY.
OrthoDBi EOG7QRQVG.
PhylomeDBi P35456.
TreeFami TF338662.

Miscellaneous databases

EvolutionaryTracei P35456.
NextBioi 295076.
PMAP-CutDB P35456.
PROi P35456.
SOURCEi Search...

Gene expression databases

Bgeei P35456.
CleanExi MM_PLAUR.
ExpressionAtlasi P35456. baseline and differential.
Genevestigatori P35456.

Family and domain databases

InterProi IPR018363. CD59_antigen_CS.
IPR016054. LY6_UPA_recep-like.
IPR001526. LY6_UPAR.
[Graphical view ]
Pfami PF00021. UPAR_LY6. 3 hits.
[Graphical view ]
SMARTi SM00134. LU. 3 hits.
[Graphical view ]
PROSITEi PS00983. LY6_UPAR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract."
    Kristensen P., Eriksen J., Blasi F., Danoe K.
    J. Cell Biol. 115:1763-1771(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
    Tissue: Macrophage.
  2. "The murine urokinase-type plasminogen activator receptor gene."
    Suh T.T., Nerlov C., Dano K., Degen J.L.
    J. Biol. Chem. 269:25992-25998(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: INTERACTION WITH SRPX2, TISSUE SPECIFICITY.
  4. "Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy."
    Lin L., Gardsvoll H., Huai Q., Huang M., Ploug M.
    J. Biol. Chem. 285:10982-10992(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 24-300 IN COMPLEX WITH PLAU, DISULFIDE BONDS, GLYCOSYLATION AT ASN-75; ASN-193 AND ASN-282.

Entry informationi

Entry nameiUPAR_MOUSE
AccessioniPrimary (citable) accession number: P35456
Secondary accession number(s): P35457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3