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P35456

- UPAR_MOUSE

UniProt

P35456 - UPAR_MOUSE

Protein

Urokinase plasminogen activator surface receptor

Gene

Plaur

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urokinase plasminogen activator surface receptor
    Short name:
    U-PAR
    Short name:
    uPAR
    Alternative name(s):
    CD_antigen: CD87
    Gene namesi
    Name:Plaur
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

    Organism-specific databases

    MGIiMGI:97612. Plaur.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-SubCell
    3. integral component of plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – ?298275Urokinase plasminogen activator surface receptorPRO_0000036096Add
    BLAST
    Propeptidei?299 – 32729Removed in mature formSequence AnalysisPRO_0000036097Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 471 Publication
    Disulfide bondi29 ↔ 351 Publication
    Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi40 ↔ 681 Publication
    Glycosylationi75 – 751N-linked (GlcNAc...)1 Publication
    Disulfide bondi94 ↔ 991 Publication
    Disulfide bondi119 ↔ 1461 Publication
    Disulfide bondi122 ↔ 1291 Publication
    Disulfide bondi139 ↔ 1681 Publication
    Disulfide bondi174 ↔ 1911 Publication
    Glycosylationi183 – 1831N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi192 ↔ 1971 Publication
    Glycosylationi193 – 1931N-linked (GlcNAc...)1 Publication
    Disulfide bondi215 ↔ 2431 Publication
    Disulfide bondi218 ↔ 2261 Publication
    Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi236 ↔ 2621 Publication
    Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi268 ↔ 2871 Publication
    Glycosylationi282 – 2821N-linked (GlcNAc...)1 Publication
    Disulfide bondi288 ↔ 2931 Publication
    Lipidationi298 – 2981GPI-anchor amidated glycineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP35456.
    PRIDEiP35456.

    Miscellaneous databases

    PMAP-CutDBP35456.

    Expressioni

    Tissue specificityi

    Expressed in angiogenic endothelial cells (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP35456.
    BgeeiP35456.
    CleanExiMM_PLAUR.
    GenevestigatoriP35456.

    Interactioni

    Subunit structurei

    Monomer Probable. Interacts (via the UPAR/Ly6 domains) with SRPX2. Interacts with MRC2 By similarity. Interacts with SORL1 By similarity.By similarityCurated

    Protein-protein interaction databases

    IntActiP35456. 1 interaction.

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi25 – 295
    Beta strandi36 – 394
    Beta strandi47 – 5610
    Beta strandi61 – 688
    Beta strandi76 – 827
    Beta strandi85 – 917
    Beta strandi118 – 1203
    Turni124 – 1274
    Beta strandi129 – 1324
    Beta strandi136 – 1383
    Beta strandi146 – 1538
    Beta strandi163 – 1708
    Beta strandi175 – 1817
    Beta strandi183 – 19412
    Turni195 – 1984
    Beta strandi214 – 2185
    Beta strandi220 – 23011
    Beta strandi232 – 2354
    Beta strandi242 – 2465
    Beta strandi259 – 2635
    Beta strandi265 – 2717
    Helixi272 – 2754
    Beta strandi285 – 2917
    Helixi292 – 2943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LAQX-ray3.20U/V24-300[»]
    ProteinModelPortaliP35456.
    SMRiP35456. Positions 24-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35456.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 11794UPAR/Ly6 1Add
    BLAST
    Domaini117 – 21296UPAR/Ly6 2Add
    BLAST
    Domaini213 – 29886UPAR/Ly6 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 UPAR/Ly6 domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG41870.
    HOGENOMiHOG000136855.
    HOVERGENiHBG000245.
    InParanoidiP35456.
    KOiK03985.
    OMAiTNRTMSY.
    OrthoDBiEOG7QRQVG.
    PhylomeDBiP35456.
    TreeFamiTF338662.

    Family and domain databases

    InterProiIPR018363. CD59_antigen_CS.
    IPR016054. LY6_UPA_recep-like.
    IPR001526. LY6_UPAR.
    [Graphical view]
    PfamiPF00021. UPAR_LY6. 3 hits.
    [Graphical view]
    SMARTiSM00134. LU. 3 hits.
    [Graphical view]
    PROSITEiPS00983. LY6_UPAR. 3 hits.
    [Graphical view]
    ProtoNetiSearch...

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P35456-1) [UniParc]FASTAAdd to Basket

    Also known as: GPI-anchored

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGLPRRLLLL LLLATTCVPA SQGLQCMQCE SNQSCLVEEC ALGQDLCRTT    50
    VLREWQDDRE LEVVTRGCAH SEKTNRTMSY RMGSMIISLT ETVCATNLCN 100
    RPRPGARGRA FPQGRYLECA SCTSLDQSCE RGREQSLQCR YPTEHCIEVV 150
    TLQSTERSLK DEDYTRGCGS LPGCPGTAGF HSNQTFHFLK CCNYTHCNGG 200
    PVLDLQSFPP NGFQCYSCEG NNTLGCSSEE ASLINCRGPM NQCLVATGLD 250
    VLGNRSYTVR GCATASWCQG SHVADSFPTH LNVSVSCCHG SGCNSPTGGA 300
    PRPGPAQLSL IASLLLTLGL WGVLLWT 327

    Note: GPI-anchored form.

    Length:327
    Mass (Da):35,428
    Last modified:June 1, 1994 - v1
    Checksum:iA117441D738B6343
    GO
    Isoform 2 (identifier: P35456-2) [UniParc] [UniParc]FASTAAdd to Basket

    Also known as: Secreted

    The sequence of this isoform differs from the canonical sequence as follows:
         157-222: RSLKDEDYTR...FQCYSCEGNN → SKLPSAGQLL...LVMSRILLSF
         223-327: Missing.

    Show »
    Length:222
    Mass (Da):24,619
    Checksum:i8BA6B9F21389C587
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei157 – 22266RSLKD…CEGNN → SKLPSAGQLLVEIFKSWEQS ASKRQLNPHTVTGPTFSVTG SSGSLDQLGSDQEPSYLVMS RILLSF in isoform 2. 1 PublicationVSP_031837Add
    BLAST
    Alternative sequencei223 – 327105Missing in isoform 2. 1 PublicationVSP_031838Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62700 mRNA. Translation: CAA44574.1.
    X62701 mRNA. Translation: CAA44575.1.
    U12235 Genomic DNA. Translation: AAB60484.1.
    CCDSiCCDS20950.1. [P35456-1]
    PIRiA55356.
    B41643.
    RefSeqiNP_035243.1. NM_011113.3. [P35456-1]
    XP_006539702.1. XM_006539639.1. [P35456-1]
    XP_006539703.1. XM_006539640.1. [P35456-1]
    XP_006539704.1. XM_006539641.1. [P35456-1]
    UniGeneiMm.1359.

    Genome annotation databases

    EnsembliENSMUST00000002284; ENSMUSP00000002284; ENSMUSG00000046223. [P35456-1]
    GeneIDi18793.
    KEGGimmu:18793.
    UCSCiuc009fpr.1. mouse. [P35456-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62700 mRNA. Translation: CAA44574.1 .
    X62701 mRNA. Translation: CAA44575.1 .
    U12235 Genomic DNA. Translation: AAB60484.1 .
    CCDSi CCDS20950.1. [P35456-1 ]
    PIRi A55356.
    B41643.
    RefSeqi NP_035243.1. NM_011113.3. [P35456-1 ]
    XP_006539702.1. XM_006539639.1. [P35456-1 ]
    XP_006539703.1. XM_006539640.1. [P35456-1 ]
    XP_006539704.1. XM_006539641.1. [P35456-1 ]
    UniGenei Mm.1359.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LAQ X-ray 3.20 U/V 24-300 [» ]
    ProteinModelPortali P35456.
    SMRi P35456. Positions 24-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35456. 1 interaction.

    Proteomic databases

    PaxDbi P35456.
    PRIDEi P35456.

    Protocols and materials databases

    DNASUi 18793.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002284 ; ENSMUSP00000002284 ; ENSMUSG00000046223 . [P35456-1 ]
    GeneIDi 18793.
    KEGGi mmu:18793.
    UCSCi uc009fpr.1. mouse. [P35456-1 ]

    Organism-specific databases

    CTDi 5329.
    MGIi MGI:97612. Plaur.

    Phylogenomic databases

    eggNOGi NOG41870.
    HOGENOMi HOG000136855.
    HOVERGENi HBG000245.
    InParanoidi P35456.
    KOi K03985.
    OMAi TNRTMSY.
    OrthoDBi EOG7QRQVG.
    PhylomeDBi P35456.
    TreeFami TF338662.

    Miscellaneous databases

    EvolutionaryTracei P35456.
    NextBioi 295076.
    PMAP-CutDB P35456.
    PROi P35456.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35456.
    Bgeei P35456.
    CleanExi MM_PLAUR.
    Genevestigatori P35456.

    Family and domain databases

    InterProi IPR018363. CD59_antigen_CS.
    IPR016054. LY6_UPA_recep-like.
    IPR001526. LY6_UPAR.
    [Graphical view ]
    Pfami PF00021. UPAR_LY6. 3 hits.
    [Graphical view ]
    SMARTi SM00134. LU. 3 hits.
    [Graphical view ]
    PROSITEi PS00983. LY6_UPAR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract."
      Kristensen P., Eriksen J., Blasi F., Danoe K.
      J. Cell Biol. 115:1763-1771(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
      Tissue: Macrophage.
    2. "The murine urokinase-type plasminogen activator receptor gene."
      Suh T.T., Nerlov C., Dano K., Degen J.L.
      J. Biol. Chem. 269:25992-25998(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: INTERACTION WITH SRPX2, TISSUE SPECIFICITY.
    4. "Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy."
      Lin L., Gardsvoll H., Huai Q., Huang M., Ploug M.
      J. Biol. Chem. 285:10982-10992(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 24-300 IN COMPLEX WITH PLAU, DISULFIDE BONDS, GLYCOSYLATION AT ASN-75; ASN-193 AND ASN-282.

    Entry informationi

    Entry nameiUPAR_MOUSE
    AccessioniPrimary (citable) accession number: P35456
    Secondary accession number(s): P35457
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi