ID   NEU2_MOUSE              Reviewed;         168 AA.
AC   P35455;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   27-MAR-2024, entry version 178.
DE   RecName: Full=Vasopressin-neurophysin 2-copeptin;
DE   AltName: Full=AVP-NPII;
DE   Contains:
DE     RecName: Full=Arg-vasopressin;
DE     AltName: Full=Arginine-vasopressin;
DE   Contains:
DE     RecName: Full=Neurophysin 2;
DE     AltName: Full=Neurophysin-I;
DE   Contains:
DE     RecName: Full=Copeptin;
DE   Flags: Precursor;
GN   Name=Avp;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2176709; DOI=10.1016/0169-328x(90)90045-f;
RA   Hara Y., Battey J., Gainer H.;
RT   "Structure of mouse vasopressin and oxytocin genes.";
RL   Brain Res. Mol. Brain Res. 8:319-324(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Neurophysin 2 specifically binds vasopressin.
CC   -!- FUNCTION: Vasopressin has a direct antidiuretic action on the kidney,
CC       it also causes vasoconstriction of the peripheral vessels. Acts by
CC       binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and
CC       V2R/AVPR2) (By similarity). {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBUNIT: Interacts with vasopressin receptors V1bR/AVPR1B (Ki=85 pM),
CC       V1aR/AVPR1A (Ki=0.6 nM) and V2R/AVPR2 (Ki=4.9 nM) (By similarity).
CC       Interacts with oxytocin receptor (OXTR) (Ki=110 nM) (By similarity).
CC       {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR   EMBL; M88354; AAC42027.1; -; Genomic_DNA.
DR   EMBL; BC051997; AAH51997.1; -; mRNA.
DR   CCDS; CCDS16746.1; -.
DR   PIR; B43755; B43755.
DR   RefSeq; NP_033862.1; NM_009732.2.
DR   AlphaFoldDB; P35455; -.
DR   SMR; P35455; -.
DR   STRING; 10090.ENSMUSP00000035551; -.
DR   GlyConnect; 2816; 3 N-Linked glycans (1 site).
DR   GlyCosmos; P35455; 1 site, 3 glycans.
DR   GlyGen; P35455; 1 site, 3 N-linked glycans (1 site).
DR   iPTMnet; P35455; -.
DR   PhosphoSitePlus; P35455; -.
DR   CPTAC; non-CPTAC-3931; -.
DR   PaxDb; 10090-ENSMUSP00000035551; -.
DR   PeptideAtlas; P35455; -.
DR   ProteomicsDB; 287383; -.
DR   Antibodypedia; 7264; 375 antibodies from 31 providers.
DR   Ensembl; ENSMUST00000046001.7; ENSMUSP00000035551.7; ENSMUSG00000037727.7.
DR   GeneID; 11998; -.
DR   KEGG; mmu:11998; -.
DR   UCSC; uc008mjh.1; mouse.
DR   AGR; MGI:88121; -.
DR   CTD; 551; -.
DR   MGI; MGI:88121; Avp.
DR   VEuPathDB; HostDB:ENSMUSG00000037727; -.
DR   eggNOG; ENOG502S21K; Eukaryota.
DR   GeneTree; ENSGT00390000004511; -.
DR   HOGENOM; CLU_125770_0_0_1; -.
DR   InParanoid; P35455; -.
DR   OMA; SICTACY; -.
DR   OrthoDB; 5398672at2759; -.
DR   PhylomeDB; P35455; -.
DR   TreeFam; TF333018; -.
DR   Reactome; R-MMU-388479; Vasopressin-like receptors.
DR   Reactome; R-MMU-416476; G alpha (q) signalling events.
DR   Reactome; R-MMU-418555; G alpha (s) signalling events.
DR   Reactome; R-MMU-432040; Vasopressin regulates renal water homeostasis via Aquaporins.
DR   Reactome; R-MMU-879518; Transport of organic anions.
DR   Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR   Reactome; R-MMU-8856828; Clathrin-mediated endocytosis.
DR   BioGRID-ORCS; 11998; 3 hits in 77 CRISPR screens.
DR   ChiTaRS; Avp; mouse.
DR   PRO; PR:P35455; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; P35455; Protein.
DR   Bgee; ENSMUSG00000037727; Expressed in paraventricular nucleus of hypothalamus and 59 other cell types or tissues.
DR   ExpressionAtlas; P35455; baseline and differential.
DR   GO; GO:0030425; C:dendrite; ISO:MGI.
DR   GO; GO:0005576; C:extracellular region; ISO:MGI.
DR   GO; GO:0005615; C:extracellular space; ISO:MGI.
DR   GO; GO:0098992; C:neuronal dense core vesicle; ISO:MGI.
DR   GO; GO:0030141; C:secretory granule; ISO:MGI.
DR   GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; ISO:MGI.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   GO; GO:0005184; F:neuropeptide hormone activity; ISO:MGI.
DR   GO; GO:0004672; F:protein kinase activity; ISO:MGI.
DR   GO; GO:0031894; F:V1A vasopressin receptor binding; ISO:MGI.
DR   GO; GO:0031895; F:V1B vasopressin receptor binding; ISO:MGI.
DR   GO; GO:0070371; P:ERK1 and ERK2 cascade; ISO:MGI.
DR   GO; GO:0007625; P:grooming behavior; ISO:MGI.
DR   GO; GO:0007626; P:locomotory behavior; ISO:MGI.
DR   GO; GO:0002125; P:maternal aggressive behavior; ISO:MGI.
DR   GO; GO:0042711; P:maternal behavior; ISO:MGI.
DR   GO; GO:0050891; P:multicellular organismal-level water homeostasis; ISO:MGI.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR   GO; GO:0007621; P:negative regulation of female receptivity; ISO:MGI.
DR   GO; GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; ISO:MGI.
DR   GO; GO:0051970; P:negative regulation of transmission of nerve impulse; ISO:MGI.
DR   GO; GO:0030307; P:positive regulation of cell growth; ISO:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR   GO; GO:0032849; P:positive regulation of cellular pH reduction; ISO:MGI.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR   GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR   GO; GO:0014049; P:positive regulation of glutamate secretion; ISO:MGI.
DR   GO; GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; ISO:MGI.
DR   GO; GO:0031394; P:positive regulation of prostaglandin biosynthetic process; ISO:MGI.
DR   GO; GO:0003084; P:positive regulation of systemic arterial blood pressure; ISO:MGI.
DR   GO; GO:0045907; P:positive regulation of vasoconstriction; ISO:MGI.
DR   GO; GO:0070528; P:protein kinase C signaling; ISO:MGI.
DR   GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR   GO; GO:0035094; P:response to nicotine; IEA:Ensembl.
DR   GO; GO:0033574; P:response to testosterone; IEA:Ensembl.
DR   GO; GO:0007165; P:signal transduction; ISO:MGI.
DR   GO; GO:0035176; P:social behavior; ISO:MGI.
DR   GO; GO:0042310; P:vasoconstriction; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; ISO:MGI.
DR   Gene3D; 2.60.9.10; Neurohypophysial hormone domain; 1.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR   InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR   PANTHER; PTHR11681; NEUROPHYSIN; 1.
DR   PANTHER; PTHR11681:SF9; VASOPRESSIN-NEUROPHYSIN 2-COPEPTIN; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   SMART; SM00003; NH; 1.
DR   SUPFAM; SSF49606; Neurophysin II; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
DR   Genevisible; P35455; MM.
PE   2: Evidence at transcript level;
KW   Amidation; Cleavage on pair of basic residues; Disulfide bond;
KW   Glycoprotein; Hormone; Reference proteome; Secreted; Signal; Vasoactive;
KW   Vasoconstrictor.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   PEPTIDE         24..32
FT                   /note="Arg-vasopressin"
FT                   /id="PRO_0000020518"
FT   CHAIN           36..128
FT                   /note="Neurophysin 2"
FT                   /id="PRO_0000020519"
FT   PEPTIDE         130..168
FT                   /note="Copeptin"
FT                   /id="PRO_0000020520"
FT   SITE            32
FT                   /note="Important for agonist activity on V1aR/AVPR1A"
FT                   /evidence="ECO:0000250|UniProtKB:P01185"
FT   MOD_RES         32
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000250|UniProtKB:P01185"
FT   CARBOHYD        135
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        24..29
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        45..89
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        48..62
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        56..79
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        63..69
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        96..108
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        102..120
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        109..114
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
SQ   SEQUENCE   168 AA;  17900 MW;  234F6D826E0BBEE9 CRC64;
     MLARMLNTTL SACFLSLLAF SSACYFQNCP RGGKRAISDM ELRQCLPCGP GGKGRCFGPS
     ICCADELGCF VGTAEALRCQ EENYLPSPCQ SGQKPCGSGG RCAAVGICCS DESCVAEPEC
     HDGFFRLTRA REPSNATQLD GPARALLLRL VQLAGTRESV DSAKPRVY
//