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P35449 (NHX9_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable Na(+)/H(+) antiporter nhx-9
Alternative name(s):
Na(+)-H(+) exchanger protein 9
Gene names
Name:nhx-9
ORF Names:ZK822.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length667 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serves some physiological function other than regulation of cellular pH.

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Excretory cell.

Tissue specificity

In early stage larva, expressed in the twin excretory cell processes. At later larval stages, expression is more restricted, resulting in a "beads on a chain" appearance.

Post-translational modification

Phosphorylated Potential.

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Ontologies

Keywords
   Biological processAntiport
Ion transport
Sodium transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of pH

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral to membrane

Non-traceable author statement Ref.3. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsodium:hydrogen antiporter activity

Non-traceable author statement Ref.3. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: P35449-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: P35449-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MSSRLFVFFLICYATADKIEKELISDGFQ → MGHEYDIEE
Isoform c (identifier: P35449-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 667667Probable Na(+)/H(+) antiporter nhx-9
PRO_0000052371

Regions

Transmembrane41 – 6121Helical; Potential
Transmembrane73 – 9321Helical; Potential
Transmembrane97 – 11721Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane165 – 18521Helical; Potential
Transmembrane192 – 21221Helical; Potential
Transmembrane236 – 25621Helical; Potential
Transmembrane268 – 28821Helical; Potential
Transmembrane325 – 34521Helical; Potential
Transmembrane351 – 37121Helical; Potential
Transmembrane390 – 41021Helical; Potential
Transmembrane418 – 43821Helical; Potential

Amino acid modifications

Modified residue6441Phosphothreonine Potential
Glycosylation3101N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 6060Missing in isoform c.
VSP_003396
Alternative sequence1 – 2929MSSRL…SDGFQ → MGHEYDIEE in isoform b.
VSP_003395

Experimental info

Sequence conflict111 – 1122DA → GSS in AAA27892. Ref.3
Sequence conflict214 – 2174MFKS → CSK in AAA27892. Ref.3
Sequence conflict4151A → R in AAA27892. Ref.3
Sequence conflict4261V → W in AAA27892. Ref.3
Sequence conflict6251Missing in AAA27892. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified June 20, 2002. Version 2.
Checksum: AB4245787973135B

FASTA66775,281
        10         20         30         40         50         60 
MSSRLFVFFL ICYATADKIE KELISDGFQL FTWKWDDVHH VYVITVWLLI ASLAKILFNL 

        70         80         90        100        110        120 
MKPISKWCPD SSLLIIVGLA LGWILHQTSL SGATLDSHTF FLYLLPPIIF DAGYFMPNRA 

       130        140        150        160        170        180 
LFENFDSVLV FSVFGTIWNT FAIGGSLLLM AQYDLFTMSF TTFEILVFSA LISAVDPVAV 

       190        200        210        220        230        240 
IAVFEEIHVN EFLFINVFGE ALFNDGVTVV LYQMFKSFAL IGSENLSVLD YATGGLSFFV 

       250        260        270        280        290        300 
VALGGAAVGI IFAIAASLTT KYTYDVRILA PVFIFVLPYM AYLTAEMVSL SSIIAIAICG 

       310        320        330        340        350        360 
MLMKQYIKGN VTQAAANSVK YFTKMLAQSS ETVIFMFLGL STISSQHHFD LYFICATLFF 

       370        380        390        400        410        420 
CLIYRAIGIV VQCYILNRFR AKKFEMVDQF IMSYGGLRGA IAYGLVVSIP ASITAKPMFI 

       430        440        450        460        470        480 
TATIAVIYFT VFLQGITIRP LVNFLKIKKK EERDPTMVES VYNKYLDYMM SGVEDIAGQK 

       490        500        510        520        530        540 
GHYTFIENFE RFNAKVIKPV LMRHQKRESF DASSIVRAYE KITLEDAIKL AKVKNNIQNK 

       550        560        570        580        590        600 
RLERIKSKGR VAPILPDKIS NQKTMTPKDL QLKRFMESGE NIDSLYTLFS DLLDRKLHEM 

       610        620        630        640        650        660 
NRPSVQITDV DGQDDIQDDY MAEVGSRSNL SAMFRSTEQL PSETPFHSGR RQSTGDLNAT 


RRADFNV

« Hide

Isoform b [UniParc].

Checksum: 312B3E0E7DD59D58
Show »

FASTA64773,030
Isoform c [UniParc].

Checksum: 6C4E8CEE0F71B6D7
Show »

FASTA60768,189

References

« Hide 'large scale' references
[1]"The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."
Nehrke K., Melvin J.E.
J. Biol. Chem. 277:29036-29044(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Molecular analysis of two genes between let-653 and let-56 in the unc-22(IV) region of Caenorhabditis elegans."
Marra M.A., Prasad S.S., Baillie D.L.
Mol. Gen. Genet. 236:289-298(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-667 (ISOFORMS A AND B).
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF497834 mRNA. Translation: AAM18112.1.
AF497835 mRNA. Translation: AAM18113.1.
Z73898 Genomic DNA. Translation: CAC42388.1.
Z73898 Genomic DNA. Translation: CAC42389.1.
Z73898 Genomic DNA. Translation: CAC42390.3.
M23064 mRNA. Translation: AAA27892.1.
RefSeqNP_001023627.1. NM_001028456.3.
NP_001023628.1. NM_001028457.3.
NP_001023629.2. NM_001028458.3.
UniGeneCel.11579.

3D structure databases

ProteinModelPortalP35449.
ModBaseSearch...

Proteomic databases

PaxDbP35449.
PRIDEP35449.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZK822.3b.1; ZK822.3b.1; ZK822.3.
ZK822.3b.2; ZK822.3b.2; ZK822.3.
GeneID178126.
KEGGcel:CELE_ZK822.3.
UCSCZK822.3b. c. elegans.

Organism-specific databases

CTD178126.
WormBaseZK822.3a; CE27559; WBGene00003736; nhx-9.
ZK822.3b; CE27560; WBGene00003736; nhx-9.
ZK822.3c; CE29015; WBGene00003736; nhx-9.

Phylogenomic databases

eggNOGCOG0025.
GeneTreeENSGT00560000077158.
HOGENOMHOG000247044.
InParanoidP35449.
OMAACGVIMR.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018416. Na/H_exchanger_3/9.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF19. PTHR10110:SF19. 1 hit.
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSPR01084. NAHEXCHNGR.
TIGRFAMsTIGR00840. b_cpa1. 1 hit.
ProtoNetSearch...

Other

NextBio899818.

Entry information

Entry nameNHX9_CAEEL
AccessionPrimary (citable) accession number: P35449
Secondary accession number(s): Q95PV8, Q95PV9, Q95PW0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 20, 2002
Last modified: April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents