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P35448 (TSP1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thrombospondin-1
Gene names
Name:thbs1
Synonyms:tsp1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length1173 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3 By similarity.

Subunit structure

Homotrimer; disulfide-linked.

Sequence similarities

Belongs to the thrombospondin family.

Contains 2 EGF-like domains.

Contains 1 laminin G-like domain.

Contains 1 TSP C-terminal (TSPC) domain.

Contains 3 TSP type-1 domains.

Contains 8 TSP type-3 repeats.

Contains 1 VWFC domain.

Ontologies

Keywords
   Biological processCell adhesion
   DomainEGF-like domain
Repeat
Signal
   LigandCalcium
Heparin-binding
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 11731155Thrombospondin-1
PRO_0000035844

Regions

Domain22 – 224203Laminin G-like
Domain319 – 37658VWFC
Domain382 – 43251TSP type-1 1
Domain438 – 49356TSP type-1 2
Domain495 – 55056TSP type-1 3
Domain550 – 59041EGF-like 1
Domain649 – 69345EGF-like 2
Repeat694 – 72936TSP type-3 1
Repeat730 – 76536TSP type-3 2
Repeat766 – 78823TSP type-3 3
Repeat789 – 82436TSP type-3 4
Repeat825 – 84723TSP type-3 5
Repeat848 – 88538TSP type-3 6
Repeat886 – 92136TSP type-3 7
Repeat922 – 95736TSP type-3 8
Domain961 – 1173213TSP C-terminal
Region50 – 9849Heparin-binding By similarity
Motif929 – 9313Cell attachment site Potential

Amino acid modifications

Glycosylation1551N-linked (GlcNAc...) Potential
Glycosylation1581N-linked (GlcNAc...) Potential
Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation3631N-linked (GlcNAc...) Potential
Glycosylation7051N-linked (GlcNAc...) Potential
Glycosylation7111N-linked (GlcNAc...) Potential
Glycosylation10701N-linked (GlcNAc...) Potential
Disulfide bond174 ↔ 235 By similarity
Disulfide bond394 ↔ 426 By similarity
Disulfide bond398 ↔ 431 By similarity
Disulfide bond409 ↔ 416 By similarity
Disulfide bond450 ↔ 487 By similarity
Disulfide bond454 ↔ 492 By similarity
Disulfide bond465 ↔ 477 By similarity
Disulfide bond507 ↔ 544 By similarity
Disulfide bond511 ↔ 549 By similarity
Disulfide bond522 ↔ 534 By similarity
Disulfide bond554 ↔ 565 By similarity
Disulfide bond559 ↔ 575 By similarity
Disulfide bond578 ↔ 589 By similarity
Disulfide bond595 ↔ 611 By similarity
Disulfide bond602 ↔ 620 By similarity
Disulfide bond623 ↔ 647 By similarity
Disulfide bond653 ↔ 666 By similarity
Disulfide bond660 ↔ 679 By similarity
Disulfide bond681 ↔ 692 By similarity
Disulfide bond708 ↔ 716 By similarity
Disulfide bond721 ↔ 741 By similarity
Disulfide bond757 ↔ 777 By similarity
Disulfide bond780 ↔ 800 By similarity
Disulfide bond816 ↔ 836 By similarity
Disulfide bond839 ↔ 859 By similarity
Disulfide bond877 ↔ 897 By similarity
Disulfide bond913 ↔ 933 By similarity
Disulfide bond949 ↔ 1170 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35448 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: A9F036D6516C0F24

FASTA1,173130,020
        10         20         30         40         50         60 
MKGIFLLLML VMPQTHQAAE SGNDDNSVFD LFELTGYNRK AGSRKPQGLH LVKGPDPSSP 

        70         80         90        100        110        120 
AYRIEDADLI PPLPEDKFQD LLDAIRADRG FILLATLRQA KKSRGALLSV ERKDGGGHIF 

       130        140        150        160        170        180 
SLISNGRART LDLSLSGERK QQVVSVEDAV LATGNWTNIT LFVQEDRAQL YVGCNKMENA 

       190        200        210        220        230        240 
ELDVPIQKIF TENLASTAHL RVAKGGVKDN FQGVLQNVRF VFGTTLEAIL RNKGCLSMTN 

       250        260        270        280        290        300 
SVITLDNPVN GSSPAIRTNY IGHKTKDLQA VCGFSCDDLS KLFAEMKGLR TLVTTLKDQV 

       310        320        330        340        350        360 
TKETEKNELI AQIVTRTPGV CLHNGVLHKN RDEWTVDSCT ECTCQNSATI CRKVSCPLMP 

       370        380        390        400        410        420 
CTNATIPDGE CCPRCWPSDS ADDDWSPWSD WTPCSVTCGH GIQQRGRSCD SLNNPCEGSS 

       430        440        450        460        470        480 
VQTRSCQIQD CDKRFKQDGG WSHWSPWSSC SVTCGSGQIT RIRLCNSPVP QLNGKQCEGE 

       490        500        510        520        530        540 
GRENKPCQKD PCPINGQWGP WSLWDTCTVT CGGGMQKRER LCNNPKPQYE GKDCIGEPTD 

       550        560        570        580        590        600 
SQICNKQDCP IDGCLSNPCF AGVKCTSFID GSWKCGSCPP GYRGNGITCK DIDECKEVPD 

       610        620        630        640        650        660 
ACFTLNGVHR CENTEPGYNC LPCPPRFTGT QPFGKGIEEA KANKQVCKPR NPCADGTHDC 

       670        680        690        700        710        720 
HKNARCIYLG HYSDPMFRCE CRPGYAGNGI ICGEDTDLDG WPNENLTCVD NATYHCLKDN 

       730        740        750        760        770        780 
CPNLPNSGQE DYDKDGMGDA CDKDDDNDGI LDDRDNCQFV YNPAQYDYDR DDVGDRCDNC 

       790        800        810        820        830        840 
PYNHNPDQAD TDRNGEGDAC SVDIDGDGIL NERDNCAYVY NVDQKDTDKD GVGDQCDNCP 

       850        860        870        880        890        900 
LEHNPEQTDS DSDLIGDKCD NNQDIDEDGH QNNLDNCPYI PNANQADHDK DGKGDACDHD 

       910        920        930        940        950        960 
DDNDGVPDDK DNCRLVPNPD QTDTNGDGRG DACQYDFDDD SIPDAEDVCP ENVEISTTDF 

       970        980        990       1000       1010       1020 
RKFQMVPLDP KGTSQIDPNW VVRHQGKELV QTVNCDPGIA VGFDEFSAVD FSGTFFINTE 

      1030       1040       1050       1060       1070       1080 
RDDDYAGFVF GYQSSSRFYV VMWKQITQTY WDTTPTVAQG YSGLSIKVVN STSGPGEHLR 

      1090       1100       1110       1120       1130       1140 
NALWHTGNTP GQVRTLWHDP HQKGWKDFTA YRWHLTHRPK TGFIRVVMYE GKRVMADSGP 

      1150       1160       1170 
IYDKTYAGGR LGLFVFSQEM VFFSDLKYEC RDS

« Hide

References

[1]"Cloning, characterization and expression of thrombospondin-1 in Xenopus laevis embryos."
Urry L.A., Ramos J., Duquette M., Desimone D.W., Lawler J.
Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L04278 mRNA. No translation available.

3D structure databases

ProteinModelPortalP35448.
SMRP35448. Positions 25-235, 552-1172.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

XenbaseXB-GENE-865586. thbs1.

Phylogenomic databases

HOVERGENHBG018006.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR026823. cEGF.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR001007. VWF_C.
[Graphical view]
PfamPF12662. cEGF. 1 hit.
PF12947. EGF_3. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 2 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 2 hits.
SSF82895. TSP1. 3 hits.
PROSITEPS00022. EGF_1. False negative.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50025. LAM_G_DOMAIN. False negative.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTSP1_XENLA
AccessionPrimary (citable) accession number: P35448
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 6, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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