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P35448

- TSP1_XENLA

UniProt

P35448 - TSP1_XENLA

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Protein

Thrombospondin-1

Gene

thbs1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3 (By similarity). May play a role in ER stress response (By similarity).By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. extracellular matrix binding Source: InterPro
  3. heparin binding Source: UniProtKB-KW

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. inflammatory response Source: InterPro
  3. negative regulation of angiogenesis Source: InterPro
  4. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:thbs1
Synonyms:tsp1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865586. thbs1.

Subcellular locationi

Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular space Source: InterPro
  3. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 11731155Thrombospondin-1PRO_0000035844Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi174 ↔ 235By similarity
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi394 ↔ 426By similarity
Disulfide bondi398 ↔ 431By similarity
Disulfide bondi409 ↔ 416By similarity
Disulfide bondi450 ↔ 487By similarity
Disulfide bondi454 ↔ 492By similarity
Disulfide bondi465 ↔ 477By similarity
Disulfide bondi507 ↔ 544By similarity
Disulfide bondi511 ↔ 549By similarity
Disulfide bondi522 ↔ 534By similarity
Disulfide bondi554 ↔ 565By similarity
Disulfide bondi559 ↔ 575By similarity
Disulfide bondi578 ↔ 589By similarity
Disulfide bondi595 ↔ 611By similarity
Disulfide bondi602 ↔ 620By similarity
Disulfide bondi623 ↔ 647By similarity
Disulfide bondi653 ↔ 666By similarity
Disulfide bondi660 ↔ 679By similarity
Disulfide bondi681 ↔ 692By similarity
Glycosylationi705 – 7051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi708 ↔ 716By similarity
Glycosylationi711 – 7111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi721 ↔ 741By similarity
Disulfide bondi757 ↔ 777By similarity
Disulfide bondi780 ↔ 800By similarity
Disulfide bondi816 ↔ 836By similarity
Disulfide bondi839 ↔ 859By similarity
Disulfide bondi877 ↔ 897By similarity
Disulfide bondi913 ↔ 933By similarity
Disulfide bondi949 ↔ 1170By similarity
Glycosylationi1070 – 10701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; disulfide-linked.

Structurei

3D structure databases

ProteinModelPortaliP35448.
SMRiP35448. Positions 25-235, 552-1172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 224203Laminin G-likeAdd
BLAST
Domaini319 – 37658VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini382 – 43251TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini438 – 49356TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini495 – 55056TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini550 – 59041EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini649 – 69345EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Repeati694 – 72936TSP type-3 1Add
BLAST
Repeati730 – 76536TSP type-3 2Add
BLAST
Repeati766 – 78823TSP type-3 3Add
BLAST
Repeati789 – 82436TSP type-3 4Add
BLAST
Repeati825 – 84723TSP type-3 5Add
BLAST
Repeati848 – 88538TSP type-3 6Add
BLAST
Repeati886 – 92136TSP type-3 7Add
BLAST
Repeati922 – 95736TSP type-3 8Add
BLAST
Domaini961 – 1173213TSP C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 9849Heparin-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi929 – 9313Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

HOVERGENiHBG018006.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR026823. cEGF.
IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF12662. cEGF. 1 hit.
PF12947. EGF_3. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35448-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGIFLLLML VMPQTHQAAE SGNDDNSVFD LFELTGYNRK AGSRKPQGLH
60 70 80 90 100
LVKGPDPSSP AYRIEDADLI PPLPEDKFQD LLDAIRADRG FILLATLRQA
110 120 130 140 150
KKSRGALLSV ERKDGGGHIF SLISNGRART LDLSLSGERK QQVVSVEDAV
160 170 180 190 200
LATGNWTNIT LFVQEDRAQL YVGCNKMENA ELDVPIQKIF TENLASTAHL
210 220 230 240 250
RVAKGGVKDN FQGVLQNVRF VFGTTLEAIL RNKGCLSMTN SVITLDNPVN
260 270 280 290 300
GSSPAIRTNY IGHKTKDLQA VCGFSCDDLS KLFAEMKGLR TLVTTLKDQV
310 320 330 340 350
TKETEKNELI AQIVTRTPGV CLHNGVLHKN RDEWTVDSCT ECTCQNSATI
360 370 380 390 400
CRKVSCPLMP CTNATIPDGE CCPRCWPSDS ADDDWSPWSD WTPCSVTCGH
410 420 430 440 450
GIQQRGRSCD SLNNPCEGSS VQTRSCQIQD CDKRFKQDGG WSHWSPWSSC
460 470 480 490 500
SVTCGSGQIT RIRLCNSPVP QLNGKQCEGE GRENKPCQKD PCPINGQWGP
510 520 530 540 550
WSLWDTCTVT CGGGMQKRER LCNNPKPQYE GKDCIGEPTD SQICNKQDCP
560 570 580 590 600
IDGCLSNPCF AGVKCTSFID GSWKCGSCPP GYRGNGITCK DIDECKEVPD
610 620 630 640 650
ACFTLNGVHR CENTEPGYNC LPCPPRFTGT QPFGKGIEEA KANKQVCKPR
660 670 680 690 700
NPCADGTHDC HKNARCIYLG HYSDPMFRCE CRPGYAGNGI ICGEDTDLDG
710 720 730 740 750
WPNENLTCVD NATYHCLKDN CPNLPNSGQE DYDKDGMGDA CDKDDDNDGI
760 770 780 790 800
LDDRDNCQFV YNPAQYDYDR DDVGDRCDNC PYNHNPDQAD TDRNGEGDAC
810 820 830 840 850
SVDIDGDGIL NERDNCAYVY NVDQKDTDKD GVGDQCDNCP LEHNPEQTDS
860 870 880 890 900
DSDLIGDKCD NNQDIDEDGH QNNLDNCPYI PNANQADHDK DGKGDACDHD
910 920 930 940 950
DDNDGVPDDK DNCRLVPNPD QTDTNGDGRG DACQYDFDDD SIPDAEDVCP
960 970 980 990 1000
ENVEISTTDF RKFQMVPLDP KGTSQIDPNW VVRHQGKELV QTVNCDPGIA
1010 1020 1030 1040 1050
VGFDEFSAVD FSGTFFINTE RDDDYAGFVF GYQSSSRFYV VMWKQITQTY
1060 1070 1080 1090 1100
WDTTPTVAQG YSGLSIKVVN STSGPGEHLR NALWHTGNTP GQVRTLWHDP
1110 1120 1130 1140 1150
HQKGWKDFTA YRWHLTHRPK TGFIRVVMYE GKRVMADSGP IYDKTYAGGR
1160 1170
LGLFVFSQEM VFFSDLKYEC RDS
Length:1,173
Mass (Da):130,020
Last modified:June 1, 1994 - v1
Checksum:iA9F036D6516C0F24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04278 mRNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04278 mRNA. No translation available.

3D structure databases

ProteinModelPortali P35448.
SMRi P35448. Positions 25-235, 552-1172.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Xenbasei XB-GENE-865586. thbs1.

Phylogenomic databases

HOVERGENi HBG018006.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR026823. cEGF.
IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view ]
PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
Pfami PF12662. cEGF. 1 hit.
PF12947. EGF_3. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 2 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization and expression of thrombospondin-1 in Xenopus laevis embryos."
    Urry L.A., Ramos J., Duquette M., Desimone D.W., Lawler J.
    Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTSP1_XENLA
AccessioniPrimary (citable) accession number: P35448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3