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Protein

Spondin-1

Gene

Spon1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi325 – 3251CalciumBy similarity
Metal bindingi354 – 3541CalciumBy similarity
Metal bindingi358 – 3581Calcium; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Spondin-1
Alternative name(s):
F-spondin
Gene namesi
Name:Spon1
Synonyms:Sponf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619918. Spon1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 807779Spondin-1PRO_0000035867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 128PROSITE-ProRule annotation
Disulfide bondi156 ↔ 182PROSITE-ProRule annotation
Disulfide bondi199 ↔ 336By similarity
Disulfide bondi200 ↔ 340By similarity
Disulfide bondi202 ↔ 415By similarity
Glycosylationi214 – 2141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi443 ↔ 480PROSITE-ProRule annotation1 Publication
Disulfide bondi454 ↔ 489PROSITE-ProRule annotation1 Publication
Disulfide bondi459 ↔ 494PROSITE-ProRule annotation1 Publication
Disulfide bondi502 ↔ 538PROSITE-ProRule annotation
Disulfide bondi513 ↔ 517PROSITE-ProRule annotation
Disulfide bondi548 ↔ 554PROSITE-ProRule annotation
Disulfide bondi559 ↔ 595PROSITE-ProRule annotation
Disulfide bondi570 ↔ 574PROSITE-ProRule annotation
Disulfide bondi605 ↔ 610PROSITE-ProRule annotation
Disulfide bondi615 ↔ 650PROSITE-ProRule annotation1 Publication
Disulfide bondi626 ↔ 630PROSITE-ProRule annotation1 Publication
Disulfide bondi660 ↔ 665PROSITE-ProRule annotation1 Publication
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35446.
PRIDEiP35446.

Expressioni

Tissue specificityi

Expressed at high levels in the floor plate.

Interactioni

Subunit structurei

Binds to the central extracellular domain of APP and inhibits beta-secretase cleavage of APP.

Protein-protein interaction databases

IntActiP35446. 1 interaction.
STRINGi10116.ENSRNOP00000046944.

Structurei

Secondary structure

1
807
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi441 – 4455Combined sources
Beta strandi457 – 4604Combined sources
Beta strandi470 – 4745Combined sources
Beta strandi629 – 6324Combined sources
Beta strandi634 – 6385Combined sources
Beta strandi654 – 6596Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SZLNMR-A441-499[»]
1VEXNMR-A613-666[»]
ProteinModelPortaliP35446.
SMRiP35446. Positions 441-499.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35446.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 194166ReelinPROSITE-ProRule annotationAdd
BLAST
Domaini195 – 388194SpondinPROSITE-ProRule annotationAdd
BLAST
Domaini442 – 49554TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini501 – 55555TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini558 – 61154TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini614 – 66653TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini668 – 72154TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini754 – 80653TSP type-1 6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 reelin domain.PROSITE-ProRule annotation
Contains 1 spondin domain.PROSITE-ProRule annotation
Contains 6 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3539. Eukaryota.
ENOG410XQHP. LUCA.
HOGENOMiHOG000124667.
HOVERGENiHBG052920.
InParanoidiP35446.
PhylomeDBiP35446.

Family and domain databases

CDDicd08544. Reeler. 1 hit.
InterProiIPR002861. Reeler_dom.
IPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF02014. Reeler. 1 hit.
PF06468. Spond_N. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 6 hits.
PROSITEiPS51019. REELIN. 1 hit.
PS51020. SPONDIN. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSPAPLRL SRGPALLALA LPLAAALAFS DETLDKVAKS EGYCSRILRA
60 70 80 90 100
QGTRREGYTE FSLRVEGDPD FYKPGSSYRV TLSAAPPSYF RGFTLIALKE
110 120 130 140 150
NREGDKEEDH AGTFQIIDEE ETQFMSNCPV AVTESTPRRR TRIQVFWIAP
160 170 180 190 200
PTGTGCVILK ASIVQKRIIY FQDEGSLTKK LCEQDPTLDG VTDRPILDCC
210 220 230 240 250
ACGTAKYRLT FYGNWSEKTH PKDYPRRANH WSAIIGGSHS KNYVLWEYGG
260 270 280 290 300
YASEGVKQVA ELGSPVKMEE EIRQQSDEVL TVIKAKAQWP SWQPVNVRAA
310 320 330 340 350
PSAEFSVDRT RHLMSFLTMM GPSPDWNVGL SAEDLCTKEC GWVQKVVQDL
360 370 380 390 400
IPWDAGTDSG VTYESPNKPT IPQEKIRPLT SLDHPQSPFY DPEGGSITQV
410 420 430 440 450
ARVVIERIAR KGEQCNIVPD NVDDIVADLA PEEKDEDDTP ETCIYSNWSP
460 470 480 490 500
WSACSSSTCE KGKRMRQRML KAQLDLSVPC PDTQDFQPCM GPGCSDEDGS
510 520 530 540 550
TCTMSEWITW SPCSVSCGMG MRSRERYVKQ FPEDGSVCML PTEETEKCTV
560 570 580 590 600
NEECSPSSCL VTEWGEWDDC SATCGMGMKK RHRMVKMSPA DGSMCKAETS
610 620 630 640 650
QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC
660 670 680 690 700
NEDLEQAEKC MLPECPIDCE LSEWSQWSEC NKSCGKGHMI RTRTIQMEPQ
710 720 730 740 750
FGGAPCPETV QRKKCRARKC LRSPSIQKLR WREARESRRS EQLREESDGE
760 770 780 790 800
QFPGCRMRPW TAWSECTKLC GGGIQERYMT VKKRFKSSQF TSCKDKKEIR

ACNVHPC
Length:807
Mass (Da):90,773
Last modified:June 1, 1994 - v1
Checksum:i309525F9EAFEA89A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88469 mRNA. Translation: AAA41174.1.
PIRiA38152.
RefSeqiNP_742064.1. NM_172067.1.
UniGeneiRn.7546.

Genome annotation databases

GeneIDi64456.
KEGGirno:64456.
UCSCiRGD:619918. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88469 mRNA. Translation: AAA41174.1.
PIRiA38152.
RefSeqiNP_742064.1. NM_172067.1.
UniGeneiRn.7546.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SZLNMR-A441-499[»]
1VEXNMR-A613-666[»]
ProteinModelPortaliP35446.
SMRiP35446. Positions 441-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP35446. 1 interaction.
STRINGi10116.ENSRNOP00000046944.

Proteomic databases

PaxDbiP35446.
PRIDEiP35446.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64456.
KEGGirno:64456.
UCSCiRGD:619918. rat.

Organism-specific databases

CTDi10418.
RGDi619918. Spon1.

Phylogenomic databases

eggNOGiKOG3539. Eukaryota.
ENOG410XQHP. LUCA.
HOGENOMiHOG000124667.
HOVERGENiHBG052920.
InParanoidiP35446.
PhylomeDBiP35446.

Miscellaneous databases

EvolutionaryTraceiP35446.
PROiP35446.

Family and domain databases

CDDicd08544. Reeler. 1 hit.
InterProiIPR002861. Reeler_dom.
IPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF02014. Reeler. 1 hit.
PF06468. Spond_N. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 6 hits.
PROSITEiPS51019. REELIN. 1 hit.
PS51020. SPONDIN. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPON1_RAT
AccessioniPrimary (citable) accession number: P35446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.