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P35443

- TSP4_HUMAN

UniProt

P35443 - TSP4_HUMAN

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Protein

Thrombospondin-4

Gene
THBS4, TSP4
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner By similarity.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. heparin binding Source: UniProtKB
  3. integrin binding Source: UniProtKB
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. endothelial cell-cell adhesion Source: UniProtKB
  3. myoblast migration Source: UniProtKB
  4. negative regulation of angiogenesis Source: UniProtKB
  5. positive regulation of cell division Source: UniProtKB-KW
  6. positive regulation of endothelial cell proliferation Source: UniProtKB
  7. positive regulation of neutrophil chemotaxis Source: UniProtKB
  8. positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  9. regulation of tissue remodeling Source: UniProtKB
  10. response to endoplasmic reticulum stress Source: UniProtKB
  11. response to unfolded protein Source: UniProtKB-KW
  12. tissue remodeling Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Tissue remodeling, Unfolded protein response

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_16888. Signaling by PDGF.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4
Gene namesi
Name:THBS4
Synonyms:TSP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:11788. THBS4.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB
  3. extracellular matrix Source: UniProtKB
  4. extracellular region Source: Reactome
  5. extracellular space Source: UniProtKB
  6. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36500.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed predictionAdd
BLAST
Chaini27 – 961935Thrombospondin-4PRO_0000035852Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi258 – 258Interchain Inferred
Disulfide bondi261 – 261Interchain Inferred
Disulfide bondi290 ↔ 301 By similarity
Disulfide bondi295 ↔ 310 By similarity
Disulfide bondi313 ↔ 324 By similarity
Disulfide bondi330 ↔ 341 By similarity
Disulfide bondi335 ↔ 350 By similarity
Disulfide bondi353 ↔ 377 By similarity
Disulfide bondi383 ↔ 394 By similarity
Disulfide bondi388 ↔ 403 By similarity
Disulfide bondi406 ↔ 418 By similarity
Disulfide bondi424 ↔ 438 By similarity
Disulfide bondi432 ↔ 448 By similarity
Disulfide bondi450 ↔ 461 By similarity
Disulfide bondi477 ↔ 482 By similarity
Disulfide bondi487 ↔ 507 By similarity
Disulfide bondi523 ↔ 543 By similarity
Disulfide bondi546 ↔ 566 By similarity
Disulfide bondi582 ↔ 602 By similarity
Disulfide bondi605 ↔ 625 By similarity
Glycosylationi612 – 6121N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi643 ↔ 663 By similarity
Disulfide bondi683 ↔ 703 By similarity
Disulfide bondi719 ↔ 940 By similarity
Glycosylationi941 – 9411N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35443.
PaxDbiP35443.
PeptideAtlasiP35443.
PRIDEiP35443.

PTM databases

PhosphoSiteiP35443.

Expressioni

Gene expression databases

ArrayExpressiP35443.
BgeeiP35443.
CleanExiHS_THBS4.
GenevestigatoriP35443.

Organism-specific databases

HPAiCAB004597.

Interactioni

Subunit structurei

Homopentamer; disulfide-linked. Interacts with PTBP3. Interacts with NOTCH1 By similarity. Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation.3 Publications

Protein-protein interaction databases

IntActiP35443. 1 interaction.
STRINGi9606.ENSP00000339730.

Structurei

3D structure databases

ProteinModelPortaliP35443.
SMRiP35443. Positions 218-262, 290-952.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 192166Laminin G-likeAdd
BLAST
Domaini286 – 32540EGF-like 1Add
BLAST
Domaini326 – 36338EGF-like 2; calcium-binding Reviewed predictionAdd
BLAST
Domaini379 – 41941EGF-like 3; calcium-binding Reviewed predictionAdd
BLAST
Domaini420 – 46243EGF-like 4Add
BLAST
Repeati463 – 49533TSP type-3 1Add
BLAST
Repeati496 – 53136TSP type-3 2Add
BLAST
Repeati532 – 55423TSP type-3 3Add
BLAST
Repeati555 – 59036TSP type-3 4Add
BLAST
Repeati591 – 61323TSP type-3 5Add
BLAST
Repeati614 – 65138TSP type-3 6Add
BLAST
Repeati652 – 69140TSP type-3 7Add
BLAST
Repeati692 – 72736TSP type-3 8Add
BLAST
Domaini731 – 945215TSP C-terminalAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi562 – 5643Cell attachment site Reviewed prediction

Sequence similaritiesi

Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 8 TSP type-3 repeats.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP35443.
KOiK04659.
OMAiAECQACG.
OrthoDBiEOG76QFGD.
PhylomeDBiP35443.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35443-1 [UniParc]FASTAAdd to Basket

« Hide

MLAPRGAAVL LLHLVLQRWL AAGAQATPQV FDLLPSSSQR LNPGALLPVL    50
TDPALNDLYV ISTFKLQTKS SATIFGLYSS TDNSKYFEFT VMGRLNKAIL 100
RYLKNDGKVH LVVFNNLQLA DGRRHRILLR LSNLQRGAGS LELYLDCIQV 150
DSVHNLPRAF AGPSQKPETI ELRTFQRKPQ DFLEELKLVV RGSLFQVASL 200
QDCFLQQSEP LAATGTGDFN RQFLGQMTQL NQLLGEVKDL LRQQVKETSF 250
LRNTIAECQA CGPLKFQSPT PSTVVPPAPP APPTRPPRRC DSNPCFRGVQ 300
CTDSRDGFQC GPCPEGYTGN GITCIDVDEC KYHPCYPGVH CINLSPGFRC 350
DACPVGFTGP MVQGVGISFA KSNKQVCTDI DECRNGACVP NSICVNTLGS 400
YRCGPCKPGY TGDQIRGCKA ERNCRNPELN PCSVNAQCIE ERQGDVTCVC 450
GVGWAGDGYI CGKDVDIDSY PDEELPCSAR NCKKDNCKYV PNSGQEDADR 500
DGIGDACDED ADGDGILNEQ DNCVLIHNVD QRNSDKDIFG DACDNCLSVL 550
NNDQKDTDGD GRGDACDDDM DGDGIKNILD NCPKFPNRDQ RDKDGDGVGD 600
ACDSCPDVSN PNQSDVDNDL VGDSCDTNQD SDGDGHQDST DNCPTVINSA 650
QLDTDKDGIG DECDDDDDND GIPDLVPPGP DNCRLVPNPA QEDSNSDGVG 700
DICESDFDQD QVIDRIDVCP ENAEVTLTDF RAYQTVVLDP EGDAQIDPNW 750
VVLNQGMEIV QTMNSDPGLA VGYTAFNGVD FEGTFHVNTQ TDDDYAGFIF 800
GYQDSSSFYV VMWKQTEQTY WQATPFRAVA EPGIQLKAVK SKTGPGEHLR 850
NSLWHTGDTS DQVRLLWKDS RNVGWKDKVS YRWFLQHRPQ VGYIRVRFYE 900
GSELVADSGV TIDTTMRGGR LGVFCFSQEN IIWSNLKYRC NDTIPEDFQE 950
FQTQNFDRFD N 961
Length:961
Mass (Da):105,869
Last modified:November 23, 2004 - v2
Checksum:i18F867AA5FFDA54B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551L → Q.1 Publication
Corresponds to variant rs17881847 [ dbSNP | Ensembl ].
VAR_019951
Natural varianti387 – 3871A → P Gain of function mutation; resulting in a pro-atherogenic phenotype. 3 Publications
Corresponds to variant rs1866389 [ dbSNP | Ensembl ].
VAR_019952
Natural varianti420 – 4201A → V.3 Publications
Corresponds to variant rs17882372 [ dbSNP | Ensembl ].
VAR_019953
Natural varianti646 – 6461V → I.1 Publication
Corresponds to variant rs2229396 [ dbSNP | Ensembl ].
VAR_019954
Natural varianti737 – 7371V → I.
Corresponds to variant rs2229398 [ dbSNP | Ensembl ].
VAR_052659

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961N → S no nucleotide entry 1 Publication
Sequence conflicti96 – 961N → S in CAA79635. 1 Publication
Sequence conflicti276 – 2761P → A no nucleotide entry 1 Publication
Sequence conflicti276 – 2761P → A in CAA79635. 1 Publication
Sequence conflicti737 – 7371V → G no nucleotide entry 1 Publication
Sequence conflicti737 – 7371V → G in CAA79635. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19585 mRNA. Translation: CAA79635.1.
AK313587 mRNA. Translation: BAG36356.1.
AY566253 Genomic DNA. Translation: AAS66982.1.
BC050456 mRNA. Translation: AAH50456.1.
CCDSiCCDS4049.1.
PIRiA55710. TSHUP4.
RefSeqiNP_003239.2. NM_003248.4.
UniGeneiHs.211426.

Genome annotation databases

EnsembliENST00000350881; ENSP00000339730; ENSG00000113296.
GeneIDi7060.
KEGGihsa:7060.
UCSCiuc021yaw.1. human.

Polymorphism databases

DMDMi55977790.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19585 mRNA. Translation: CAA79635.1 .
AK313587 mRNA. Translation: BAG36356.1 .
AY566253 Genomic DNA. Translation: AAS66982.1 .
BC050456 mRNA. Translation: AAH50456.1 .
CCDSi CCDS4049.1.
PIRi A55710. TSHUP4.
RefSeqi NP_003239.2. NM_003248.4.
UniGenei Hs.211426.

3D structure databases

ProteinModelPortali P35443.
SMRi P35443. Positions 218-262, 290-952.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35443. 1 interaction.
STRINGi 9606.ENSP00000339730.

PTM databases

PhosphoSitei P35443.

Polymorphism databases

DMDMi 55977790.

Proteomic databases

MaxQBi P35443.
PaxDbi P35443.
PeptideAtlasi P35443.
PRIDEi P35443.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000350881 ; ENSP00000339730 ; ENSG00000113296 .
GeneIDi 7060.
KEGGi hsa:7060.
UCSCi uc021yaw.1. human.

Organism-specific databases

CTDi 7060.
GeneCardsi GC05P079287.
HGNCi HGNC:11788. THBS4.
HPAi CAB004597.
MIMi 600715. gene.
neXtProti NX_P35443.
PharmGKBi PA36500.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000007542.
HOVERGENi HBG000636.
InParanoidi P35443.
KOi K04659.
OMAi AECQACG.
OrthoDBi EOG76QFGD.
PhylomeDBi P35443.
TreeFami TF324917.

Enzyme and pathway databases

Reactomei REACT_16888. Signaling by PDGF.

Miscellaneous databases

GeneWikii THBS4.
GenomeRNAii 7060.
NextBioi 27609.
PROi P35443.
SOURCEi Search...

Gene expression databases

ArrayExpressi P35443.
Bgeei P35443.
CleanExi HS_THBS4.
Genevestigatori P35443.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-420.
    Tissue: Heart.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-420, SUBUNIT.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-387.
    Tissue: Synovium.
  4. SeattleSNPs variation discovery resource
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-55; PRO-387; VAL-420 AND ILE-646.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: PNS.
  6. "Regulator of differentiation 1 (ROD1) binds to the amphipathic C-terminal peptide of thrombospondin-4 and is involved in its mitogenic activity."
    Sadvakassova G., Dobocan M.C., Difalco M.R., Congote L.F.
    J. Cell. Physiol. 220:672-679(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CELL PROLIFERATION, INTERACTION WITH PTBP3.
  7. Cited for: INTERACTION WITH ATF6.
  8. "ATF6 [corrected] and thrombospondin 4: the dynamic duo of the adaptive endoplasmic reticulum stress response."
    Doroudgar S., Glembotski C.C.
    Circ. Res. 112:9-12(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. Cited for: REVIEW.
  10. "Thrombospondin-4 polymorphism (A387P) predicts cardiovascular risk in postinfarction patients with high HDL cholesterol and C-reactive protein levels."
    Corsetti J.P., Ryan D., Moss A.J., McCarthy J., Goldenberg I., Zareba W., Sparks C.E.
    Thromb. Haemost. 106:1170-1178(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF VARIANT PRO-387.

Entry informationi

Entry nameiTSP4_HUMAN
AccessioniPrimary (citable) accession number: P35443
Secondary accession number(s): B2R909, Q86TG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 23, 2004
Last modified: September 3, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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