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Protein

Thrombospondin-1

Gene

Thbs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties (By similarity). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:Thbs1
Synonyms:Tsp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98737. Thbs1.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: MGI
  • endoplasmic reticulum Source: UniProtKB
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular matrix Source: UniProtKB
  • extracellular space Source: MGI
  • fibrinogen complex Source: MGI
  • platelet alpha granule Source: MGI
  • sarcoplasmic reticulum Source: UniProtKB
  • secretory granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000003584319 – 1170Thrombospondin-1Add BLAST1152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi171 ↔ 232By similarity
Glycosylationi248N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi270InterchainCurated
Disulfide bondi274InterchainCurated
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi391 ↔ 423By similarity
Disulfide bondi395 ↔ 428By similarity
Disulfide bondi406 ↔ 413By similarity
Disulfide bondi447 ↔ 484By similarity
Disulfide bondi451 ↔ 489By similarity
Disulfide bondi462 ↔ 474By similarity
Disulfide bondi504 ↔ 541By similarity
Disulfide bondi508 ↔ 546By similarity
Disulfide bondi519 ↔ 531By similarity
Disulfide bondi551 ↔ 562By similarity
Disulfide bondi556 ↔ 572By similarity
Disulfide bondi575 ↔ 586By similarity
Disulfide bondi592 ↔ 608By similarity
Disulfide bondi599 ↔ 617By similarity
Disulfide bondi620 ↔ 644By similarity
Disulfide bondi650 ↔ 663By similarity
Disulfide bondi657 ↔ 676By similarity
Disulfide bondi678 ↔ 689By similarity
Disulfide bondi705 ↔ 713By similarity
Glycosylationi708N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi718 ↔ 738By similarity
Disulfide bondi754 ↔ 774By similarity
Disulfide bondi777 ↔ 797By similarity
Disulfide bondi813 ↔ 833By similarity
Disulfide bondi836 ↔ 856By similarity
Disulfide bondi874 ↔ 894By similarity
Disulfide bondi910 ↔ 930By similarity
Disulfide bondi946 ↔ 1167By similarity
Glycosylationi1067N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35441.
PaxDbiP35441.
PeptideAtlasiP35441.
PRIDEiP35441.

PTM databases

iPTMnetiP35441.
PhosphoSitePlusiP35441.
SwissPalmiP35441.

Expressioni

Gene expression databases

CleanExiMM_THBS1.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Interacts with ATF6 (via lumenal domain).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP35441. 2 interactors.
MINTiMINT-4138704.
STRINGi10090.ENSMUSP00000044903.

Structurei

3D structure databases

ProteinModelPortaliP35441.
SMRiP35441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 270Laminin G-likeAdd BLAST215
Domaini316 – 373VWFCPROSITE-ProRule annotationAdd BLAST58
Domaini379 – 429TSP type-1 1PROSITE-ProRule annotationAdd BLAST51
Domaini435 – 490TSP type-1 2PROSITE-ProRule annotationAdd BLAST56
Domaini492 – 547TSP type-1 3PROSITE-ProRule annotationAdd BLAST56
Domaini547 – 587EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini646 – 690EGF-like 2PROSITE-ProRule annotationAdd BLAST45
Repeati691 – 726TSP type-3 1Add BLAST36
Repeati727 – 762TSP type-3 2Add BLAST36
Repeati763 – 785TSP type-3 3Add BLAST23
Repeati786 – 821TSP type-3 4Add BLAST36
Repeati822 – 844TSP type-3 5Add BLAST23
Repeati845 – 882TSP type-3 6Add BLAST38
Repeati883 – 918TSP type-3 7Add BLAST36
Repeati919 – 954TSP type-3 8Add BLAST36
Domaini958 – 1170TSP C-terminalPROSITE-ProRule annotationAdd BLAST213

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 95Heparin-bindingBy similarityAdd BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi926 – 928Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
HOVERGENiHBG018006.
InParanoidiP35441.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR000884. TSP1_rpt.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_dom.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLRGLGVL FLLHMCGSNR IPESGGDNGV FDIFELIGGA RRGPGRRLVK
60 70 80 90 100
GQDLSSPAFR IENANLIPAV PDDKFQDLLD AVWADKGFIF LASLRQMKKT
110 120 130 140 150
RGTLLAVERK DNTGQIFSVV SNGKAGTLDL SLSLPGKQQV VSVEEALLAT
160 170 180 190 200
GQWKSITLFV QEDRAQLYID CDKMESAELD VPIQSIFTRD LASVARLRVA
210 220 230 240 250
KGDVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTNVLL TLDNNVVNGS
260 270 280 290 300
SPAIRTNYIG HKTKDLQAIC GLSCDELSSM VLELKGLRTI VTTLQDSIRK
310 320 330 340 350
VTEENRELVS ELKRPPLCFH NGVQYKNNEE WTVDSCTECH CQNSVTICKK
360 370 380 390 400
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSATCGNGIQ
410 420 430 440 450
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT
460 470 480 490 500
CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP
510 520 530 540 550
WDICSVTCGG GVQRRSRLCN NPTPQFGGKD CVGDVTENQV CNKQDCPIDG
560 570 580 590 600
CLSNPCFAGA KCTSYPDGSW KCGACPPGYS GNGIQCKDVD ECKEVPDACF
610 620 630 640 650
NHNGEHRCKN TDPGYNCLPC PPRFTGSQPF GRGVEHAMAN KQVCKPRNPC
660 670 680 690 700
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN
710 720 730 740 750
ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD
760 770 780 790 800
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDK NGEGDACAVD
810 820 830 840 850
IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD
860 870 880 890 900
LIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN
910 920 930 940 950
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDNVP DIDDICPENF
960 970 980 990 1000
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY
1010 1020 1030 1040 1050
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT
1060 1070 1080 1090 1100
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI
1110 1120 1130 1140 1150
GWKDFTAYRW RLSHRPKTGY IRVVMYEGKK IMADSGPIYD KTYAGGRLGL
1160 1170
FVFSQEMVFF SDMKYECRDS
Length:1,170
Mass (Da):129,647
Last modified:June 1, 1994 - v1
Checksum:i0443E493615E7F06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1025F → L in AAA53063 (PubMed:1371115).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62470
, M62450, M62451, M62452, M62453, M62454, M62455, M62456, M62457, M62458, M62459, M62460, M62461, M62462, M62463, M62464, M62465, M62466, M62467, M62468, M62469 Genomic DNA. Translation: AAA50611.1.
M87276 mRNA. Translation: AAA53063.1.
J05606, J05605 Genomic DNA. Translation: AAA40431.1.
PIRiA40558.
UniGeneiMm.4159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62470
, M62450, M62451, M62452, M62453, M62454, M62455, M62456, M62457, M62458, M62459, M62460, M62461, M62462, M62463, M62464, M62465, M62466, M62467, M62468, M62469 Genomic DNA. Translation: AAA50611.1.
M87276 mRNA. Translation: AAA53063.1.
J05606, J05605 Genomic DNA. Translation: AAA40431.1.
PIRiA40558.
UniGeneiMm.4159.

3D structure databases

ProteinModelPortaliP35441.
SMRiP35441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP35441. 2 interactors.
MINTiMINT-4138704.
STRINGi10090.ENSMUSP00000044903.

PTM databases

iPTMnetiP35441.
PhosphoSitePlusiP35441.
SwissPalmiP35441.

Proteomic databases

MaxQBiP35441.
PaxDbiP35441.
PeptideAtlasiP35441.
PRIDEiP35441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:98737. Thbs1.

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
HOVERGENiHBG018006.
InParanoidiP35441.

Miscellaneous databases

ChiTaRSiThbs1. mouse.
PROiP35441.
SOURCEiSearch...

Gene expression databases

CleanExiMM_THBS1.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR000884. TSP1_rpt.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_dom.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSP1_MOUSE
AccessioniPrimary (citable) accession number: P35441
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.