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P35441

- TSP1_MOUSE

UniProt

P35441 - TSP1_MOUSE

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Protein

Thrombospondin-1

Gene
Thbs1, Tsp1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties By similarity. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. extracellular matrix binding Source: MGI
  3. heparin binding Source: MGI
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. blood vessel morphogenesis Source: MGI
  3. cell adhesion Source: UniProtKB-KW
  4. endocardial cushion development Source: MGI
  5. growth plate cartilage development Source: MGI
  6. inflammatory response Source: MGI
  7. negative regulation of angiogenesis Source: MGI
  8. outflow tract morphogenesis Source: MGI
  9. positive regulation of cell-substrate adhesion Source: MGI
  10. positive regulation of macrophage chemotaxis Source: BHF-UCL
  11. positive regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
  12. response to endoplasmic reticulum stress Source: UniProtKB
  13. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:Thbs1
Synonyms:Tsp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:98737. Thbs1.

Subcellular locationi

Endoplasmic reticulum. Sarcoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular space Source: MGI
  3. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 11701152Thrombospondin-1PRO_0000035843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi171 ↔ 232 By similarity
Glycosylationi248 – 2481N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi270 – 270Interchain Inferred
Disulfide bondi274 – 274Interchain Inferred
Glycosylationi360 – 3601N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi391 ↔ 423 By similarity
Disulfide bondi395 ↔ 428 By similarity
Disulfide bondi406 ↔ 413 By similarity
Disulfide bondi447 ↔ 484 By similarity
Disulfide bondi451 ↔ 489 By similarity
Disulfide bondi462 ↔ 474 By similarity
Disulfide bondi504 ↔ 541 By similarity
Disulfide bondi508 ↔ 546 By similarity
Disulfide bondi519 ↔ 531 By similarity
Disulfide bondi551 ↔ 562 By similarity
Disulfide bondi556 ↔ 572 By similarity
Disulfide bondi575 ↔ 586 By similarity
Disulfide bondi592 ↔ 608 By similarity
Disulfide bondi599 ↔ 617 By similarity
Disulfide bondi620 ↔ 644 By similarity
Disulfide bondi650 ↔ 663 By similarity
Disulfide bondi657 ↔ 676 By similarity
Disulfide bondi678 ↔ 689 By similarity
Disulfide bondi705 ↔ 713 By similarity
Glycosylationi708 – 7081N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi718 ↔ 738 By similarity
Disulfide bondi754 ↔ 774 By similarity
Disulfide bondi777 ↔ 797 By similarity
Disulfide bondi813 ↔ 833 By similarity
Disulfide bondi836 ↔ 856 By similarity
Disulfide bondi874 ↔ 894 By similarity
Disulfide bondi910 ↔ 930 By similarity
Disulfide bondi946 ↔ 1167 By similarity
Glycosylationi1067 – 10671N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35441.
PaxDbiP35441.
PRIDEiP35441.

PTM databases

PhosphoSiteiP35441.

Expressioni

Gene expression databases

CleanExiMM_THBS1.
GenevestigatoriP35441.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked By similarity. Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 By similarity. Interacts with ATF6 (via lumenal domain).1 Publication

Protein-protein interaction databases

IntActiP35441. 2 interactions.
MINTiMINT-4138704.

Structurei

3D structure databases

ProteinModelPortaliP35441.
SMRiP35441. Positions 30-233, 549-1169.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 270215Laminin G-likeAdd
BLAST
Domaini316 – 37358VWFCAdd
BLAST
Domaini379 – 42951TSP type-1 1Add
BLAST
Domaini435 – 49056TSP type-1 2Add
BLAST
Domaini492 – 54756TSP type-1 3Add
BLAST
Domaini547 – 58741EGF-like 1Add
BLAST
Domaini646 – 69045EGF-like 2Add
BLAST
Repeati691 – 72636TSP type-3 1Add
BLAST
Repeati727 – 76236TSP type-3 2Add
BLAST
Repeati763 – 78523TSP type-3 3Add
BLAST
Repeati786 – 82136TSP type-3 4Add
BLAST
Repeati822 – 84423TSP type-3 5Add
BLAST
Repeati845 – 88238TSP type-3 6Add
BLAST
Repeati883 – 91836TSP type-3 7Add
BLAST
Repeati919 – 95436TSP type-3 8Add
BLAST
Domaini958 – 1170213TSP C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 9549Heparin-binding By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi926 – 9283Cell attachment site Reviewed prediction

Sequence similaritiesi

Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOVERGENiHBG018006.
InParanoidiP35441.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
4.10.1080.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF07645. EGF_CA. 1 hit.
PF02210. Laminin_G_2. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35441-1 [UniParc]FASTAAdd to Basket

« Hide

MELLRGLGVL FLLHMCGSNR IPESGGDNGV FDIFELIGGA RRGPGRRLVK     50
GQDLSSPAFR IENANLIPAV PDDKFQDLLD AVWADKGFIF LASLRQMKKT 100
RGTLLAVERK DNTGQIFSVV SNGKAGTLDL SLSLPGKQQV VSVEEALLAT 150
GQWKSITLFV QEDRAQLYID CDKMESAELD VPIQSIFTRD LASVARLRVA 200
KGDVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTNVLL TLDNNVVNGS 250
SPAIRTNYIG HKTKDLQAIC GLSCDELSSM VLELKGLRTI VTTLQDSIRK 300
VTEENRELVS ELKRPPLCFH NGVQYKNNEE WTVDSCTECH CQNSVTICKK 350
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSATCGNGIQ 400
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT 450
CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP 500
WDICSVTCGG GVQRRSRLCN NPTPQFGGKD CVGDVTENQV CNKQDCPIDG 550
CLSNPCFAGA KCTSYPDGSW KCGACPPGYS GNGIQCKDVD ECKEVPDACF 600
NHNGEHRCKN TDPGYNCLPC PPRFTGSQPF GRGVEHAMAN KQVCKPRNPC 650
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN 700
ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD 750
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDK NGEGDACAVD 800
IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD 850
LIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN 900
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDNVP DIDDICPENF 950
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY 1000
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT 1050
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI 1100
GWKDFTAYRW RLSHRPKTGY IRVVMYEGKK IMADSGPIYD KTYAGGRLGL 1150
FVFSQEMVFF SDMKYECRDS 1170
Length:1,170
Mass (Da):129,647
Last modified:June 1, 1994 - v1
Checksum:i0443E493615E7F06
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1025 – 10251F → L in AAA53063. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62470
, M62450, M62451, M62452, M62453, M62454, M62455, M62456, M62457, M62458, M62459, M62460, M62461, M62462, M62463, M62464, M62465, M62466, M62467, M62468, M62469 Genomic DNA. Translation: AAA50611.1.
M87276 mRNA. Translation: AAA53063.1.
J05606, J05605 Genomic DNA. Translation: AAA40431.1.
PIRiA40558.
UniGeneiMm.4159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62470
, M62450 , M62451 , M62452 , M62453 , M62454 , M62455 , M62456 , M62457 , M62458 , M62459 , M62460 , M62461 , M62462 , M62463 , M62464 , M62465 , M62466 , M62467 , M62468 , M62469 Genomic DNA. Translation: AAA50611.1 .
M87276 mRNA. Translation: AAA53063.1 .
J05606 , J05605 Genomic DNA. Translation: AAA40431.1 .
PIRi A40558.
UniGenei Mm.4159.

3D structure databases

ProteinModelPortali P35441.
SMRi P35441. Positions 30-233, 549-1169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35441. 2 interactions.
MINTi MINT-4138704.

PTM databases

PhosphoSitei P35441.

Proteomic databases

MaxQBi P35441.
PaxDbi P35441.
PRIDEi P35441.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:98737. Thbs1.

Phylogenomic databases

eggNOGi NOG12793.
HOVERGENi HBG018006.
InParanoidi P35441.

Miscellaneous databases

ChiTaRSi THBS1. mouse.
PROi P35441.
SOURCEi Search...

Gene expression databases

CleanExi MM_THBS1.
Genevestigatori P35441.

Family and domain databases

Gene3Di 2.60.120.200. 3 hits.
4.10.1080.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view ]
PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
Pfami PF07645. EGF_CA. 1 hit.
PF02210. Laminin_G_2. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development."
    Laherty C.D., O'Rourke K., Wolf F.W., Katz R., Seldin M.F., Dixit V.M.
    J. Biol. Chem. 267:3274-3281(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression."
    Bornstein P., Alfi D., Devarayalu S., Framson P., Li P.
    J. Biol. Chem. 265:16691-16698(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-490.
  4. "Expression and initial characterization of recombinant mouse thrombospondin 1 and thrombospondin 3."
    Chen H., Aeschlimann D., Nowlen J., Mosher D.F.
    FEBS Lett. 387:36-41(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-37.
  5. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1067.
    Tissue: Myoblast.
  6. Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATF6.

Entry informationi

Entry nameiTSP1_MOUSE
AccessioniPrimary (citable) accession number: P35441
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi