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P35441

- TSP1_MOUSE

UniProt

P35441 - TSP1_MOUSE

Protein

Thrombospondin-1

Gene

Thbs1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties By similarity. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.By similarity1 Publication

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. extracellular matrix binding Source: MGI
    3. heparin binding Source: MGI
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. behavioral response to pain Source: UniProtKB
    2. blood vessel morphogenesis Source: MGI
    3. cell adhesion Source: UniProtKB-KW
    4. endocardial cushion development Source: MGI
    5. growth plate cartilage development Source: MGI
    6. inflammatory response Source: MGI
    7. negative regulation of angiogenesis Source: MGI
    8. outflow tract morphogenesis Source: MGI
    9. positive regulation of cell-substrate adhesion Source: MGI
    10. positive regulation of macrophage chemotaxis Source: BHF-UCL
    11. positive regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
    12. response to endoplasmic reticulum stress Source: UniProtKB
    13. response to unfolded protein Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion, Unfolded protein response

    Keywords - Ligandi

    Calcium, Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombospondin-1
    Gene namesi
    Name:Thbs1
    Synonyms:Tsp1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:98737. Thbs1.

    Subcellular locationi

    Endoplasmic reticulum 1 Publication. Sarcoplasmic reticulum 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. extracellular space Source: MGI
    3. sarcoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Sarcoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 11701152Thrombospondin-1PRO_0000035843Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi171 ↔ 232By similarity
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi270 – 270InterchainCurated
    Disulfide bondi274 – 274InterchainCurated
    Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi391 ↔ 423By similarity
    Disulfide bondi395 ↔ 428By similarity
    Disulfide bondi406 ↔ 413By similarity
    Disulfide bondi447 ↔ 484By similarity
    Disulfide bondi451 ↔ 489By similarity
    Disulfide bondi462 ↔ 474By similarity
    Disulfide bondi504 ↔ 541By similarity
    Disulfide bondi508 ↔ 546By similarity
    Disulfide bondi519 ↔ 531By similarity
    Disulfide bondi551 ↔ 562By similarity
    Disulfide bondi556 ↔ 572By similarity
    Disulfide bondi575 ↔ 586By similarity
    Disulfide bondi592 ↔ 608By similarity
    Disulfide bondi599 ↔ 617By similarity
    Disulfide bondi620 ↔ 644By similarity
    Disulfide bondi650 ↔ 663By similarity
    Disulfide bondi657 ↔ 676By similarity
    Disulfide bondi678 ↔ 689By similarity
    Disulfide bondi705 ↔ 713By similarity
    Glycosylationi708 – 7081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi718 ↔ 738By similarity
    Disulfide bondi754 ↔ 774By similarity
    Disulfide bondi777 ↔ 797By similarity
    Disulfide bondi813 ↔ 833By similarity
    Disulfide bondi836 ↔ 856By similarity
    Disulfide bondi874 ↔ 894By similarity
    Disulfide bondi910 ↔ 930By similarity
    Disulfide bondi946 ↔ 1167By similarity
    Glycosylationi1067 – 10671N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP35441.
    PaxDbiP35441.
    PRIDEiP35441.

    PTM databases

    PhosphoSiteiP35441.

    Expressioni

    Gene expression databases

    CleanExiMM_THBS1.
    GenevestigatoriP35441.

    Interactioni

    Subunit structurei

    Homotrimer; disulfide-linked By similarity. Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 By similarity. Interacts with ATF6 (via lumenal domain).By similarity1 Publication

    Protein-protein interaction databases

    IntActiP35441. 2 interactions.
    MINTiMINT-4138704.

    Structurei

    3D structure databases

    ProteinModelPortaliP35441.
    SMRiP35441. Positions 30-233, 549-1169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 270215Laminin G-likeAdd
    BLAST
    Domaini316 – 37358VWFCPROSITE-ProRule annotationAdd
    BLAST
    Domaini379 – 42951TSP type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini435 – 49056TSP type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini492 – 54756TSP type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini547 – 58741EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini646 – 69045EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati691 – 72636TSP type-3 1Add
    BLAST
    Repeati727 – 76236TSP type-3 2Add
    BLAST
    Repeati763 – 78523TSP type-3 3Add
    BLAST
    Repeati786 – 82136TSP type-3 4Add
    BLAST
    Repeati822 – 84423TSP type-3 5Add
    BLAST
    Repeati845 – 88238TSP type-3 6Add
    BLAST
    Repeati883 – 91836TSP type-3 7Add
    BLAST
    Repeati919 – 95436TSP type-3 8Add
    BLAST
    Domaini958 – 1170213TSP C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni47 – 9549Heparin-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi926 – 9283Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Belongs to the thrombospondin family.Curated
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 laminin G-like domain.Curated
    Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
    Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
    Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
    Contains 1 VWFC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    HOVERGENiHBG018006.
    InParanoidiP35441.

    Family and domain databases

    Gene3Di2.60.120.200. 3 hits.
    4.10.1080.10. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR001791. Laminin_G.
    IPR028499. Thrombospondin-1.
    IPR000884. Thrombospondin_1_rpt.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    IPR001007. VWF_C.
    [Graphical view]
    PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
    PfamiPF07645. EGF_CA. 1 hit.
    PF02210. Laminin_G_2. 1 hit.
    PF00090. TSP_1. 3 hits.
    PF02412. TSP_3. 7 hits.
    PF05735. TSP_C. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 3 hits.
    SM00209. TSP1. 3 hits.
    SM00210. TSPN. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view]
    SUPFAMiSSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    SSF82895. SSF82895. 3 hits.
    PROSITEiPS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS50092. TSP1. 3 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35441-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELLRGLGVL FLLHMCGSNR IPESGGDNGV FDIFELIGGA RRGPGRRLVK     50
    GQDLSSPAFR IENANLIPAV PDDKFQDLLD AVWADKGFIF LASLRQMKKT 100
    RGTLLAVERK DNTGQIFSVV SNGKAGTLDL SLSLPGKQQV VSVEEALLAT 150
    GQWKSITLFV QEDRAQLYID CDKMESAELD VPIQSIFTRD LASVARLRVA 200
    KGDVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTNVLL TLDNNVVNGS 250
    SPAIRTNYIG HKTKDLQAIC GLSCDELSSM VLELKGLRTI VTTLQDSIRK 300
    VTEENRELVS ELKRPPLCFH NGVQYKNNEE WTVDSCTECH CQNSVTICKK 350
    VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSATCGNGIQ 400
    QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT 450
    CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP 500
    WDICSVTCGG GVQRRSRLCN NPTPQFGGKD CVGDVTENQV CNKQDCPIDG 550
    CLSNPCFAGA KCTSYPDGSW KCGACPPGYS GNGIQCKDVD ECKEVPDACF 600
    NHNGEHRCKN TDPGYNCLPC PPRFTGSQPF GRGVEHAMAN KQVCKPRNPC 650
    TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN 700
    ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD 750
    RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDK NGEGDACAVD 800
    IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD 850
    LIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN 900
    DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDNVP DIDDICPENF 950
    DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY 1000
    DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT 1050
    NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI 1100
    GWKDFTAYRW RLSHRPKTGY IRVVMYEGKK IMADSGPIYD KTYAGGRLGL 1150
    FVFSQEMVFF SDMKYECRDS 1170
    Length:1,170
    Mass (Da):129,647
    Last modified:June 1, 1994 - v1
    Checksum:i0443E493615E7F06
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1025 – 10251F → L in AAA53063. (PubMed:1371115)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62470
    , M62450, M62451, M62452, M62453, M62454, M62455, M62456, M62457, M62458, M62459, M62460, M62461, M62462, M62463, M62464, M62465, M62466, M62467, M62468, M62469 Genomic DNA. Translation: AAA50611.1.
    M87276 mRNA. Translation: AAA53063.1.
    J05606, J05605 Genomic DNA. Translation: AAA40431.1.
    PIRiA40558.
    UniGeneiMm.4159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62470
    , M62450 , M62451 , M62452 , M62453 , M62454 , M62455 , M62456 , M62457 , M62458 , M62459 , M62460 , M62461 , M62462 , M62463 , M62464 , M62465 , M62466 , M62467 , M62468 , M62469 Genomic DNA. Translation: AAA50611.1 .
    M87276 mRNA. Translation: AAA53063.1 .
    J05606 , J05605 Genomic DNA. Translation: AAA40431.1 .
    PIRi A40558.
    UniGenei Mm.4159.

    3D structure databases

    ProteinModelPortali P35441.
    SMRi P35441. Positions 30-233, 549-1169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35441. 2 interactions.
    MINTi MINT-4138704.

    PTM databases

    PhosphoSitei P35441.

    Proteomic databases

    MaxQBi P35441.
    PaxDbi P35441.
    PRIDEi P35441.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:98737. Thbs1.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOVERGENi HBG018006.
    InParanoidi P35441.

    Miscellaneous databases

    ChiTaRSi THBS1. mouse.
    PROi P35441.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_THBS1.
    Genevestigatori P35441.

    Family and domain databases

    Gene3Di 2.60.120.200. 3 hits.
    4.10.1080.10. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR001791. Laminin_G.
    IPR028499. Thrombospondin-1.
    IPR000884. Thrombospondin_1_rpt.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    IPR001007. VWF_C.
    [Graphical view ]
    PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
    Pfami PF07645. EGF_CA. 1 hit.
    PF02210. Laminin_G_2. 1 hit.
    PF00090. TSP_1. 3 hits.
    PF02412. TSP_3. 7 hits.
    PF05735. TSP_C. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 3 hits.
    SM00209. TSP1. 3 hits.
    SM00210. TSPN. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    SSF82895. SSF82895. 3 hits.
    PROSITEi PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS50092. TSP1. 3 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development."
      Laherty C.D., O'Rourke K., Wolf F.W., Katz R., Seldin M.F., Dixit V.M.
      J. Biol. Chem. 267:3274-3281(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression."
      Bornstein P., Alfi D., Devarayalu S., Framson P., Li P.
      J. Biol. Chem. 265:16691-16698(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-490.
    4. "Expression and initial characterization of recombinant mouse thrombospondin 1 and thrombospondin 3."
      Chen H., Aeschlimann D., Nowlen J., Mosher D.F.
      FEBS Lett. 387:36-41(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-37.
    5. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1067.
      Tissue: Myoblast.
    6. Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATF6.

    Entry informationi

    Entry nameiTSP1_MOUSE
    AccessioniPrimary (citable) accession number: P35441
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3