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P35441

- TSP1_MOUSE

UniProt

P35441 - TSP1_MOUSE

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Protein

Thrombospondin-1

Gene

Thbs1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties (By similarity). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.By similarity1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. extracellular matrix binding Source: MGI
  3. heparin binding Source: MGI

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. blood vessel morphogenesis Source: MGI
  3. cell adhesion Source: UniProtKB-KW
  4. endocardial cushion development Source: MGI
  5. growth plate cartilage development Source: MGI
  6. inflammatory response Source: MGI
  7. negative regulation of angiogenesis Source: MGI
  8. outflow tract morphogenesis Source: MGI
  9. positive regulation of cell-substrate adhesion Source: MGI
  10. positive regulation of macrophage chemotaxis Source: BHF-UCL
  11. positive regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
  12. response to endoplasmic reticulum stress Source: UniProtKB
  13. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:Thbs1
Synonyms:Tsp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:98737. Thbs1.

Subcellular locationi

Endoplasmic reticulum 1 Publication. Sarcoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular matrix Source: UniProtKB
  3. extracellular space Source: MGI
  4. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 11701152Thrombospondin-1PRO_0000035843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi171 ↔ 232By similarity
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi270 – 270InterchainCurated
Disulfide bondi274 – 274InterchainCurated
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi391 ↔ 423By similarity
Disulfide bondi395 ↔ 428By similarity
Disulfide bondi406 ↔ 413By similarity
Disulfide bondi447 ↔ 484By similarity
Disulfide bondi451 ↔ 489By similarity
Disulfide bondi462 ↔ 474By similarity
Disulfide bondi504 ↔ 541By similarity
Disulfide bondi508 ↔ 546By similarity
Disulfide bondi519 ↔ 531By similarity
Disulfide bondi551 ↔ 562By similarity
Disulfide bondi556 ↔ 572By similarity
Disulfide bondi575 ↔ 586By similarity
Disulfide bondi592 ↔ 608By similarity
Disulfide bondi599 ↔ 617By similarity
Disulfide bondi620 ↔ 644By similarity
Disulfide bondi650 ↔ 663By similarity
Disulfide bondi657 ↔ 676By similarity
Disulfide bondi678 ↔ 689By similarity
Disulfide bondi705 ↔ 713By similarity
Glycosylationi708 – 7081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi718 ↔ 738By similarity
Disulfide bondi754 ↔ 774By similarity
Disulfide bondi777 ↔ 797By similarity
Disulfide bondi813 ↔ 833By similarity
Disulfide bondi836 ↔ 856By similarity
Disulfide bondi874 ↔ 894By similarity
Disulfide bondi910 ↔ 930By similarity
Disulfide bondi946 ↔ 1167By similarity
Glycosylationi1067 – 10671N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35441.
PaxDbiP35441.
PRIDEiP35441.

PTM databases

PhosphoSiteiP35441.

Expressioni

Gene expression databases

CleanExiMM_THBS1.
GenevestigatoriP35441.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Interacts with ATF6 (via lumenal domain).By similarity1 Publication

Protein-protein interaction databases

IntActiP35441. 2 interactions.
MINTiMINT-4138704.

Structurei

3D structure databases

ProteinModelPortaliP35441.
SMRiP35441. Positions 30-233, 549-1169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 270215Laminin G-likeAdd
BLAST
Domaini316 – 37358VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini379 – 42951TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini435 – 49056TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini492 – 54756TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini547 – 58741EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini646 – 69045EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Repeati691 – 72636TSP type-3 1Add
BLAST
Repeati727 – 76236TSP type-3 2Add
BLAST
Repeati763 – 78523TSP type-3 3Add
BLAST
Repeati786 – 82136TSP type-3 4Add
BLAST
Repeati822 – 84423TSP type-3 5Add
BLAST
Repeati845 – 88238TSP type-3 6Add
BLAST
Repeati883 – 91836TSP type-3 7Add
BLAST
Repeati919 – 95436TSP type-3 8Add
BLAST
Domaini958 – 1170213TSP C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 9549Heparin-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi926 – 9283Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOVERGENiHBG018006.
InParanoidiP35441.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF07645. EGF_CA. 1 hit.
PF02210. Laminin_G_2. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35441-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELLRGLGVL FLLHMCGSNR IPESGGDNGV FDIFELIGGA RRGPGRRLVK
60 70 80 90 100
GQDLSSPAFR IENANLIPAV PDDKFQDLLD AVWADKGFIF LASLRQMKKT
110 120 130 140 150
RGTLLAVERK DNTGQIFSVV SNGKAGTLDL SLSLPGKQQV VSVEEALLAT
160 170 180 190 200
GQWKSITLFV QEDRAQLYID CDKMESAELD VPIQSIFTRD LASVARLRVA
210 220 230 240 250
KGDVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTNVLL TLDNNVVNGS
260 270 280 290 300
SPAIRTNYIG HKTKDLQAIC GLSCDELSSM VLELKGLRTI VTTLQDSIRK
310 320 330 340 350
VTEENRELVS ELKRPPLCFH NGVQYKNNEE WTVDSCTECH CQNSVTICKK
360 370 380 390 400
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSATCGNGIQ
410 420 430 440 450
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT
460 470 480 490 500
CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP
510 520 530 540 550
WDICSVTCGG GVQRRSRLCN NPTPQFGGKD CVGDVTENQV CNKQDCPIDG
560 570 580 590 600
CLSNPCFAGA KCTSYPDGSW KCGACPPGYS GNGIQCKDVD ECKEVPDACF
610 620 630 640 650
NHNGEHRCKN TDPGYNCLPC PPRFTGSQPF GRGVEHAMAN KQVCKPRNPC
660 670 680 690 700
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN
710 720 730 740 750
ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD
760 770 780 790 800
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDK NGEGDACAVD
810 820 830 840 850
IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD
860 870 880 890 900
LIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN
910 920 930 940 950
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDNVP DIDDICPENF
960 970 980 990 1000
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY
1010 1020 1030 1040 1050
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT
1060 1070 1080 1090 1100
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI
1110 1120 1130 1140 1150
GWKDFTAYRW RLSHRPKTGY IRVVMYEGKK IMADSGPIYD KTYAGGRLGL
1160 1170
FVFSQEMVFF SDMKYECRDS
Length:1,170
Mass (Da):129,647
Last modified:June 1, 1994 - v1
Checksum:i0443E493615E7F06
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1025 – 10251F → L in AAA53063. (PubMed:1371115)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62470
, M62450, M62451, M62452, M62453, M62454, M62455, M62456, M62457, M62458, M62459, M62460, M62461, M62462, M62463, M62464, M62465, M62466, M62467, M62468, M62469 Genomic DNA. Translation: AAA50611.1.
M87276 mRNA. Translation: AAA53063.1.
J05606, J05605 Genomic DNA. Translation: AAA40431.1.
PIRiA40558.
UniGeneiMm.4159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62470
, M62450 , M62451 , M62452 , M62453 , M62454 , M62455 , M62456 , M62457 , M62458 , M62459 , M62460 , M62461 , M62462 , M62463 , M62464 , M62465 , M62466 , M62467 , M62468 , M62469 Genomic DNA. Translation: AAA50611.1 .
M87276 mRNA. Translation: AAA53063.1 .
J05606 , J05605 Genomic DNA. Translation: AAA40431.1 .
PIRi A40558.
UniGenei Mm.4159.

3D structure databases

ProteinModelPortali P35441.
SMRi P35441. Positions 30-233, 549-1169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35441. 2 interactions.
MINTi MINT-4138704.

PTM databases

PhosphoSitei P35441.

Proteomic databases

MaxQBi P35441.
PaxDbi P35441.
PRIDEi P35441.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:98737. Thbs1.

Phylogenomic databases

eggNOGi NOG12793.
HOVERGENi HBG018006.
InParanoidi P35441.

Miscellaneous databases

ChiTaRSi Thbs1. mouse.
PROi P35441.
SOURCEi Search...

Gene expression databases

CleanExi MM_THBS1.
Genevestigatori P35441.

Family and domain databases

Gene3Di 2.60.120.200. 3 hits.
4.10.1080.10. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view ]
PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
Pfami PF07645. EGF_CA. 1 hit.
PF02210. Laminin_G_2. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development."
    Laherty C.D., O'Rourke K., Wolf F.W., Katz R., Seldin M.F., Dixit V.M.
    J. Biol. Chem. 267:3274-3281(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression."
    Bornstein P., Alfi D., Devarayalu S., Framson P., Li P.
    J. Biol. Chem. 265:16691-16698(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-490.
  4. "Expression and initial characterization of recombinant mouse thrombospondin 1 and thrombospondin 3."
    Chen H., Aeschlimann D., Nowlen J., Mosher D.F.
    FEBS Lett. 387:36-41(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-37.
  5. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1067.
    Tissue: Myoblast.
  6. Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATF6.

Entry informationi

Entry nameiTSP1_MOUSE
AccessioniPrimary (citable) accession number: P35441
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3