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P35438

- NMDZ1_MOUSE

UniProt

P35438 - NMDZ1_MOUSE

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Protein
Glutamate receptor ionotropic, NMDA 1
Gene
Grin1, Glurz1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Modulated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei523 – 5231Glycine By similarity
Binding sitei688 – 6881Glycine By similarity
Binding sitei732 – 7321Glycine By similarity

GO - Molecular functioni

  1. N-methyl-D-aspartate selective glutamate receptor activity Source: MGI
  2. calcium channel activity Source: MGI
  3. calcium ion binding Source: MGI
  4. calmodulin binding Source: MGI
  5. cation channel activity Source: MGI
  6. extracellular-glutamate-gated ion channel activity Source: RefGenome
  7. glutamate binding Source: Ensembl
  8. glycine binding Source: UniProtKB
  9. neurotransmitter binding Source: Ensembl
  10. protein binding Source: IntAct
  11. receptor binding Source: MGI
  12. voltage-gated cation channel activity Source: Ensembl

GO - Biological processi

  1. adult locomotory behavior Source: MGI
  2. associative learning Source: MGI
  3. calcium ion homeostasis Source: MGI
  4. calcium ion transmembrane transport Source: GOC
  5. calcium ion transport Source: MGI
  6. cation transport Source: MGI
  7. cellular calcium ion homeostasis Source: MGI
  8. cellular response to manganese ion Source: Ensembl
  9. cerebral cortex development Source: MGI
  10. conditioned taste aversion Source: MGI
  11. ion transmembrane transport Source: GOC
  12. ionotropic glutamate receptor signaling pathway Source: MGI
  13. learning Source: MGI
  14. learning or memory Source: MGI
  15. long-term memory Source: MGI
  16. male mating behavior Source: MGI
  17. memory Source: MGI
  18. negative regulation of neuron apoptotic process Source: MGI
  19. neuromuscular process Source: MGI
  20. olfactory learning Source: MGI
  21. pons maturation Source: MGI
  22. positive regulation of apoptotic process Source: MGI
  23. positive regulation of excitatory postsynaptic membrane potential Source: Ensembl
  24. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  25. prepulse inhibition Source: MGI
  26. propylene metabolic process Source: BHF-UCL
  27. protein tetramerization Source: Ensembl
  28. regulation of axonogenesis Source: MGI
  29. regulation of cell communication Source: MGI
  30. regulation of dendrite morphogenesis Source: MGI
  31. regulation of excitatory postsynaptic membrane potential Source: MGI
  32. regulation of long-term neuronal synaptic plasticity Source: MGI
  33. regulation of membrane potential Source: MGI
  34. regulation of neuron apoptotic process Source: MGI
  35. regulation of neuronal synaptic plasticity Source: MGI
  36. regulation of respiratory gaseous exchange Source: MGI
  37. regulation of synapse assembly Source: MGI
  38. regulation of synaptic plasticity Source: MGI
  39. respiratory gaseous exchange Source: MGI
  40. response to amphetamine Source: MGI
  41. response to calcium ion Source: Ensembl
  42. response to ethanol Source: Ensembl
  43. response to fungicide Source: Ensembl
  44. response to morphine Source: MGI
  45. rhythmic process Source: Ensembl
  46. sensory perception of pain Source: MGI
  47. social behavior Source: MGI
  48. startle response Source: MGI
  49. suckling behavior Source: MGI
  50. synaptic transmission Source: MGI
  51. synaptic transmission, glutamatergic Source: MGI
  52. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Enzyme and pathway databases

ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 1
Short name:
GluN1
Alternative name(s):
Glutamate [NMDA] receptor subunit zeta-1
N-methyl-D-aspartate receptor subunit NR1
Short name:
NMD-R1
Gene namesi
Name:Grin1
Synonyms:Glurz1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:95819. Grin1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity
Note: Enriched in postsynaptic plasma membrane and postsynaptic densities By similarity.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 559541Extracellular Reviewed prediction
Add
BLAST
Transmembranei560 – 58021Helical; Reviewed prediction
Add
BLAST
Topological domaini581 – 63656Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei637 – 65721Helical; Reviewed prediction
Add
BLAST
Topological domaini658 – 812155Extracellular Reviewed prediction
Add
BLAST
Transmembranei813 – 83321Helical; Reviewed prediction
Add
BLAST
Topological domaini834 – 938105Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. N-methyl-D-aspartate selective glutamate receptor complex Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. cell surface Source: BHF-UCL
  4. cytoplasm Source: MGI
  5. dendrite Source: MGI
  6. dendrite membrane Source: Ensembl
  7. dendritic spine Source: MGI
  8. endoplasmic reticulum Source: MGI
  9. excitatory synapse Source: Ensembl
  10. growth cone Source: UniProtKB
  11. membrane Source: MGI
  12. neuronal postsynaptic density Source: MGI
  13. postsynaptic density Source: MGI
  14. postsynaptic membrane Source: MGI
  15. synapse Source: MGI
  16. synaptic cleft Source: Ensembl
  17. synaptic vesicle Source: MGI
  18. terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 938920Glutamate receptor ionotropic, NMDA 1
PRO_0000011588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi79 – 79Interchain By similarity
Glycosylationi203 – 2031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi239 – 2391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi276 – 2761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi300 – 3001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi350 – 3501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi368 – 3681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi440 – 4401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi471 – 4711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi491 – 4911N-linked (GlcNAc...) Reviewed prediction
Modified residuei889 – 8891Phosphoserine; by PKC By similarity
Modified residuei890 – 8901Phosphoserine By similarity
Modified residuei896 – 8961Phosphoserine; by PKC By similarity
Modified residuei897 – 8971Phosphoserine; by PKC By similarity

Post-translational modificationi

NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP35438.
PaxDbiP35438.
PRIDEiP35438.

PTM databases

PhosphoSiteiP35438.

Expressioni

Gene expression databases

BgeeiP35438.
CleanExiMM_GRIN1.
GenevestigatoriP35438.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Interacts with DLG4 and MPDZ By similarity. Interacts with LRFN1 and LRFN2 By similarity. Interacts with MYZAP. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q6210810EBI-400084,EBI-300895
Lrp8Q924X64EBI-400084,EBI-432319

Protein-protein interaction databases

BioGridi200067. 82 interactions.
DIPiDIP-31577N.
IntActiP35438. 124 interactions.
MINTiMINT-135802.

Structurei

3D structure databases

ProteinModelPortaliP35438.
SMRiP35438. Positions 24-838.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni516 – 5183Glycine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG282132.
GeneTreeiENSGT00740000115410.
HOGENOMiHOG000231491.
HOVERGENiHBG052638.
KOiK05208.
TreeFamiTF351405.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR018882. CaM-bd_C0_NMDA_rcpt_NR1.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF10562. CaM_bdg_C0. 1 hit.
PF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P35438-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSTMHLLTFA LLFSCSFARA ACDPKIVNIG AVLSTRKHEQ MFREAVNQAN    50
KRHGSWKIQL NATSVTHKPN AIQMALSVCE DLISSQVYAI LVSHPPTPND 100
HFTPTPVSYT AGFYRIPVLG LTTRMSIYSD KSIHLSFLRT VPPYSHQSSV 150
WFEMMRVYNW NHIILLVSDD HEGRAAQKRL ETLLEERESK AEKVLQFDPG 200
TKNVTALLME ARDLEARVII LSASEDDAAT VYRAAAMLNM TGSGYVWLVG 250
EREISGNALR YAPDGIIGLQ LINGKNESAH ISDAVGVVAQ AVHELLEKEN 300
ITDPPRGCVG NTNIWKTGPL FKRVLMSSKY ADGVTGRVEF NEDGDRKFAN 350
YSIMNLQNRK LVQVGIYNGT HVIPNDRKII WPGGETEKPR GYQMSTRLKI 400
VTIHQEPFVY VKPTMSDGTC KEEFTVNGDP VKKVICTGPN DTSPGSPRHT 450
VPQCCYGFCV DLLIKLARTM NFTYEVHLVA DGKFGTQERV NNSNKKEWNG 500
MMGELLSGQA DMIVAPLTIN NERAQYIEFS KPFKYQGLTI LVKKEIPRST 550
LDSFMQPFQS TLWLLVGLSV HVVAVMLYLL DRFSPFGRFK VNSEEEEEDA 600
LTLSSAMWFS WGVLLNSGIG EGAPRSFSAR ILGMVWAGFA MIIVASYTAN 650
LAAFLVLDRP EERITGINDP RLRNPSDKFI YATVKQSSVD IYFRRQVELS 700
TMYRHMEKHN YESAAEAIQA VRDNKLHAFI WDSAVLEFEA SQKCDLVTTG 750
ELFFRSGFGI GMRKDSPWKQ NVSLSILKSH ENGFMEDLDK TWVRYQECDS 800
RSNAPATLTF ENMAGVFMLV AGGIVAGIFL IFIEIAYKRH KDARRKQMQL 850
AFAAVNVWRK NLQDRKSGRA EPDPKKKATF RAITSTLASS FKRRRSSKDT 900
STGGGRGALQ NQKDTVLPRR AIEREEGQLQ LCSRHRES 938
Length:938
Mass (Da):105,481
Last modified:June 1, 1994 - v1
Checksum:iC610632DD3E06171
GO
Isoform 2 (identifier: P35438-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     864-885: DRKSGRAEPDPKKKATFRAITS → QYHPTDITGPLNLSDPSVSTVV
     886-938: Missing.

Show »
Length:885
Mass (Da):99,423
Checksum:i283AA14096E81558
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti936 – 9361R → T.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei864 – 88522DRKSG…RAITS → QYHPTDITGPLNLSDPSVST VV in isoform 2.
VSP_014222Add
BLAST
Alternative sequencei886 – 93853Missing in isoform 2.
VSP_014223Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10028 mRNA. Translation: BAA00920.1.
AL732309 Genomic DNA. Translation: CAM14685.1.
AL732309 Genomic DNA. Translation: CAM14688.1.
BC039157 mRNA. Translation: AAH39157.1.
CCDSiCCDS15764.1. [P35438-1]
CCDS50528.1. [P35438-2]
PIRiS21104.
RefSeqiNP_001171127.1. NM_001177656.2.
NP_001171128.1. NM_001177657.2. [P35438-2]
NP_032195.1. NM_008169.3. [P35438-1]
UniGeneiMm.278672.

Genome annotation databases

EnsembliENSMUST00000028335; ENSMUSP00000028335; ENSMUSG00000026959. [P35438-1]
ENSMUST00000114312; ENSMUSP00000109951; ENSMUSG00000026959. [P35438-2]
GeneIDi14810.
KEGGimmu:14810.
UCSCiuc008iri.2. mouse. [P35438-2]
uc008irk.2. mouse. [P35438-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10028 mRNA. Translation: BAA00920.1 .
AL732309 Genomic DNA. Translation: CAM14685.1 .
AL732309 Genomic DNA. Translation: CAM14688.1 .
BC039157 mRNA. Translation: AAH39157.1 .
CCDSi CCDS15764.1. [P35438-1 ]
CCDS50528.1. [P35438-2 ]
PIRi S21104.
RefSeqi NP_001171127.1. NM_001177656.2.
NP_001171128.1. NM_001177657.2. [P35438-2 ]
NP_032195.1. NM_008169.3. [P35438-1 ]
UniGenei Mm.278672.

3D structure databases

ProteinModelPortali P35438.
SMRi P35438. Positions 24-838.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200067. 82 interactions.
DIPi DIP-31577N.
IntActi P35438. 124 interactions.
MINTi MINT-135802.

Chemistry

BindingDBi P35438.
GuidetoPHARMACOLOGYi 455.

PTM databases

PhosphoSitei P35438.

Proteomic databases

MaxQBi P35438.
PaxDbi P35438.
PRIDEi P35438.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028335 ; ENSMUSP00000028335 ; ENSMUSG00000026959 . [P35438-1 ]
ENSMUST00000114312 ; ENSMUSP00000109951 ; ENSMUSG00000026959 . [P35438-2 ]
GeneIDi 14810.
KEGGi mmu:14810.
UCSCi uc008iri.2. mouse. [P35438-2 ]
uc008irk.2. mouse. [P35438-1 ]

Organism-specific databases

CTDi 2902.
MGIi MGI:95819. Grin1.

Phylogenomic databases

eggNOGi NOG282132.
GeneTreei ENSGT00740000115410.
HOGENOMi HOG000231491.
HOVERGENi HBG052638.
KOi K05208.
TreeFami TF351405.

Enzyme and pathway databases

Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.

Miscellaneous databases

NextBioi 286995.
PROi P35438.
SOURCEi Search...

Gene expression databases

Bgeei P35438.
CleanExi MM_GRIN1.
Genevestigatori P35438.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR018882. CaM-bd_C0_NMDA_rcpt_NR1.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF10562. CaM_bdg_C0. 1 hit.
PF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and modulation of a mouse NMDA receptor subunit."
    Yamazaki M., Mori H., Araki K., Mori K.J., Mishina M.
    FEBS Lett. 300:39-45(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Eye.
  4. "Cloning and characterization of a novel NMDA receptor subunit NR3B: a dominant subunit that reduces calcium permeability."
    Matsuda K., Kamiya Y., Matsuda S., Yuzaki M.
    Brain Res. Mol. Brain Res. 100:43-52(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN2A OR GRIN2B AND GRIN3B.
  5. "Specific assembly with the NMDA receptor 3B subunit controls surface expression and calcium permeability of NMDA receptors."
    Matsuda K., Fletcher M., Kamiya Y., Yuzaki M.
    J. Neurosci. 23:10064-10073(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN2A OR GRIN2B AND GRIN3B.
  6. "GRINL1A colocalizes with N-methyl D-aspartate receptor NR1 subunit and reduces N-methyl D-aspartate toxicity."
    Roginski R.S., Goubaeva F., Mikami M., Fried-Cassorla E., Nair M.R., Yang J.
    NeuroReport 19:1721-1726(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYZAP.
  7. "Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome."
    Wang X., Zhao Y., Zhang X., Badie H., Zhou Y., Mu Y., Loo L.S., Cai L., Thompson R.C., Yang B., Chen Y., Johnson P.F., Wu C., Bu G., Mobley W.C., Zhang D., Gage F.H., Ranscht B.
    , Zhang Y.W., Lipton S.A., Hong W., Xu H.
    Nat. Med. 19:473-480(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX27.

Entry informationi

Entry nameiNMDZ1_MOUSE
AccessioniPrimary (citable) accession number: P35438
Secondary accession number(s): A2AI15, A2AI18, Q8CFS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi