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Protein

Glutamate receptor ionotropic, NMDA 1

Gene

Grin1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Modulated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei523GlycineBy similarity1
Binding sitei688GlycineBy similarity1
Binding sitei732GlycineBy similarity1

GO - Molecular functioni

  • calcium channel activity Source: MGI
  • calcium ion binding Source: MGI
  • calmodulin binding Source: MGI
  • cation channel activity Source: MGI
  • extracellular-glutamate-gated ion channel activity Source: InterPro
  • glutamate binding Source: MGI
  • glycine binding Source: UniProtKB
  • NMDA glutamate receptor activity Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

  • adult locomotory behavior Source: MGI
  • associative learning Source: MGI
  • calcium ion homeostasis Source: MGI
  • calcium ion transport Source: MGI
  • cation transport Source: MGI
  • cellular calcium ion homeostasis Source: MGI
  • cerebral cortex development Source: MGI
  • chemical synaptic transmission Source: MGI
  • conditioned taste aversion Source: MGI
  • ephrin receptor signaling pathway Source: Reactome
  • excitatory postsynaptic potential Source: MGI
  • ionotropic glutamate receptor signaling pathway Source: MGI
  • learning Source: MGI
  • learning or memory Source: MGI
  • long-term memory Source: MGI
  • male mating behavior Source: MGI
  • memory Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • neuromuscular process Source: MGI
  • olfactory learning Source: MGI
  • pons maturation Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • prepulse inhibition Source: MGI
  • propylene metabolic process Source: BHF-UCL
  • regulation of axonogenesis Source: MGI
  • regulation of cell communication Source: MGI
  • regulation of dendrite morphogenesis Source: MGI
  • regulation of long-term neuronal synaptic plasticity Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of neuronal synaptic plasticity Source: MGI
  • regulation of neuron apoptotic process Source: MGI
  • regulation of respiratory gaseous exchange Source: MGI
  • regulation of synapse assembly Source: MGI
  • regulation of synaptic plasticity Source: MGI
  • respiratory gaseous exchange Source: MGI
  • response to amphetamine Source: MGI
  • response to ethanol Source: MGI
  • response to morphine Source: MGI
  • sensory perception of pain Source: MGI
  • social behavior Source: MGI
  • startle response Source: MGI
  • suckling behavior Source: MGI
  • synaptic transmission, glutamatergic Source: MGI
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Enzyme and pathway databases

ReactomeiR-MMU-3928662. EPHB-mediated forward signaling.
R-MMU-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-MMU-442729. CREB phosphorylation through the activation of CaMKII.
R-MMU-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-8849932. SALM protein interactions at the synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 1
Short name:
GluN1
Alternative name(s):
Glutamate [NMDA] receptor subunit zeta-1
N-methyl-D-aspartate receptor subunit NR1
Short name:
NMD-R1
Gene namesi
Name:Grin1
Synonyms:Glurz1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95819. Grin1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 559ExtracellularSequence analysisAdd BLAST541
Transmembranei560 – 580HelicalSequence analysisAdd BLAST21
Topological domaini581 – 636CytoplasmicSequence analysisAdd BLAST56
Transmembranei637 – 657HelicalSequence analysisAdd BLAST21
Topological domaini658 – 812ExtracellularSequence analysisAdd BLAST155
Transmembranei813 – 833HelicalSequence analysisAdd BLAST21
Topological domaini834 – 938CytoplasmicSequence analysisAdd BLAST105

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell surface Source: BHF-UCL
  • cytoplasm Source: MGI
  • dendrite Source: CACAO
  • dendritic spine Source: MGI
  • endoplasmic reticulum Source: MGI
  • growth cone Source: UniProtKB
  • integral component of plasma membrane Source: MGI
  • membrane Source: MGI
  • NMDA selective glutamate receptor complex Source: MGI
  • plasma membrane Source: Reactome
  • postsynaptic density Source: MGI
  • postsynaptic membrane Source: MGI
  • synapse Source: MGI
  • synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001158819 – 938Glutamate receptor ionotropic, NMDA 1Add BLAST920

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi79InterchainBy similarity
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Glycosylationi350N-linked (GlcNAc...)Sequence analysis1
Glycosylationi368N-linked (GlcNAc...)Sequence analysis1
Glycosylationi440N-linked (GlcNAc...)Sequence analysis1
Glycosylationi471N-linked (GlcNAc...)Sequence analysis1
Glycosylationi491N-linked (GlcNAc...)Sequence analysis1
Modified residuei889Phosphoserine; by PKCBy similarity1
Modified residuei890PhosphoserineBy similarity1
Modified residuei896Phosphoserine; by PKCBy similarity1
Modified residuei897Phosphoserine; by PKCBy similarity1

Post-translational modificationi

NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP35438.
PaxDbiP35438.
PeptideAtlasiP35438.
PRIDEiP35438.

PTM databases

iPTMnetiP35438.
PhosphoSitePlusiP35438.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026959.
CleanExiMM_GRIN1.
ExpressionAtlasiP35438. baseline and differential.
GenevisibleiP35438. MM.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Interacts with DLG4 and MPDZ (By similarity). Interacts with LRFN1 and LRFN2 (By similarity). Interacts with MYZAP. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q6210810EBI-400084,EBI-300895
Lrp8Q924X64EBI-400084,EBI-432319

GO - Molecular functioni

  • calmodulin binding Source: MGI
  • receptor binding Source: MGI

Protein-protein interaction databases

BioGridi200067. 80 interactors.
DIPiDIP-31577N.
IntActiP35438. 124 interactors.
MINTiMINT-135802.
STRINGi10090.ENSMUSP00000028335.

Structurei

3D structure databases

ProteinModelPortaliP35438.
SMRiP35438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni516 – 518Glycine bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000231491.
HOVERGENiHBG052638.
InParanoidiP35438.
KOiK05208.
TreeFamiTF351405.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR018882. CaM-bd_C0_NMDA_rcpt_NR1.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF10562. CaM_bdg_C0. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35438-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTMHLLTFA LLFSCSFARA ACDPKIVNIG AVLSTRKHEQ MFREAVNQAN
60 70 80 90 100
KRHGSWKIQL NATSVTHKPN AIQMALSVCE DLISSQVYAI LVSHPPTPND
110 120 130 140 150
HFTPTPVSYT AGFYRIPVLG LTTRMSIYSD KSIHLSFLRT VPPYSHQSSV
160 170 180 190 200
WFEMMRVYNW NHIILLVSDD HEGRAAQKRL ETLLEERESK AEKVLQFDPG
210 220 230 240 250
TKNVTALLME ARDLEARVII LSASEDDAAT VYRAAAMLNM TGSGYVWLVG
260 270 280 290 300
EREISGNALR YAPDGIIGLQ LINGKNESAH ISDAVGVVAQ AVHELLEKEN
310 320 330 340 350
ITDPPRGCVG NTNIWKTGPL FKRVLMSSKY ADGVTGRVEF NEDGDRKFAN
360 370 380 390 400
YSIMNLQNRK LVQVGIYNGT HVIPNDRKII WPGGETEKPR GYQMSTRLKI
410 420 430 440 450
VTIHQEPFVY VKPTMSDGTC KEEFTVNGDP VKKVICTGPN DTSPGSPRHT
460 470 480 490 500
VPQCCYGFCV DLLIKLARTM NFTYEVHLVA DGKFGTQERV NNSNKKEWNG
510 520 530 540 550
MMGELLSGQA DMIVAPLTIN NERAQYIEFS KPFKYQGLTI LVKKEIPRST
560 570 580 590 600
LDSFMQPFQS TLWLLVGLSV HVVAVMLYLL DRFSPFGRFK VNSEEEEEDA
610 620 630 640 650
LTLSSAMWFS WGVLLNSGIG EGAPRSFSAR ILGMVWAGFA MIIVASYTAN
660 670 680 690 700
LAAFLVLDRP EERITGINDP RLRNPSDKFI YATVKQSSVD IYFRRQVELS
710 720 730 740 750
TMYRHMEKHN YESAAEAIQA VRDNKLHAFI WDSAVLEFEA SQKCDLVTTG
760 770 780 790 800
ELFFRSGFGI GMRKDSPWKQ NVSLSILKSH ENGFMEDLDK TWVRYQECDS
810 820 830 840 850
RSNAPATLTF ENMAGVFMLV AGGIVAGIFL IFIEIAYKRH KDARRKQMQL
860 870 880 890 900
AFAAVNVWRK NLQDRKSGRA EPDPKKKATF RAITSTLASS FKRRRSSKDT
910 920 930
STGGGRGALQ NQKDTVLPRR AIEREEGQLQ LCSRHRES
Length:938
Mass (Da):105,481
Last modified:June 1, 1994 - v1
Checksum:iC610632DD3E06171
GO
Isoform 2 (identifier: P35438-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     864-885: DRKSGRAEPDPKKKATFRAITS → QYHPTDITGPLNLSDPSVSTVV
     886-938: Missing.

Show »
Length:885
Mass (Da):99,423
Checksum:i283AA14096E81558
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti936R → T.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014222864 – 885DRKSG…RAITS → QYHPTDITGPLNLSDPSVST VV in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_014223886 – 938Missing in isoform 2. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10028 mRNA. Translation: BAA00920.1.
AL732309 Genomic DNA. Translation: CAM14685.1.
AL732309 Genomic DNA. Translation: CAM14688.1.
BC039157 mRNA. Translation: AAH39157.1.
CCDSiCCDS15764.1. [P35438-1]
CCDS50528.1. [P35438-2]
PIRiS21104.
RefSeqiNP_001171127.1. NM_001177656.2.
NP_001171128.1. NM_001177657.2. [P35438-2]
NP_032195.1. NM_008169.3. [P35438-1]
UniGeneiMm.278672.

Genome annotation databases

EnsembliENSMUST00000028335; ENSMUSP00000028335; ENSMUSG00000026959. [P35438-1]
ENSMUST00000114312; ENSMUSP00000109951; ENSMUSG00000026959. [P35438-2]
GeneIDi14810.
KEGGimmu:14810.
UCSCiuc008iri.3. mouse. [P35438-2]
uc008irk.3. mouse. [P35438-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10028 mRNA. Translation: BAA00920.1.
AL732309 Genomic DNA. Translation: CAM14685.1.
AL732309 Genomic DNA. Translation: CAM14688.1.
BC039157 mRNA. Translation: AAH39157.1.
CCDSiCCDS15764.1. [P35438-1]
CCDS50528.1. [P35438-2]
PIRiS21104.
RefSeqiNP_001171127.1. NM_001177656.2.
NP_001171128.1. NM_001177657.2. [P35438-2]
NP_032195.1. NM_008169.3. [P35438-1]
UniGeneiMm.278672.

3D structure databases

ProteinModelPortaliP35438.
SMRiP35438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200067. 80 interactors.
DIPiDIP-31577N.
IntActiP35438. 124 interactors.
MINTiMINT-135802.
STRINGi10090.ENSMUSP00000028335.

PTM databases

iPTMnetiP35438.
PhosphoSitePlusiP35438.

Proteomic databases

MaxQBiP35438.
PaxDbiP35438.
PeptideAtlasiP35438.
PRIDEiP35438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028335; ENSMUSP00000028335; ENSMUSG00000026959. [P35438-1]
ENSMUST00000114312; ENSMUSP00000109951; ENSMUSG00000026959. [P35438-2]
GeneIDi14810.
KEGGimmu:14810.
UCSCiuc008iri.3. mouse. [P35438-2]
uc008irk.3. mouse. [P35438-1]

Organism-specific databases

CTDi2902.
MGIiMGI:95819. Grin1.

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000231491.
HOVERGENiHBG052638.
InParanoidiP35438.
KOiK05208.
TreeFamiTF351405.

Enzyme and pathway databases

ReactomeiR-MMU-3928662. EPHB-mediated forward signaling.
R-MMU-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-MMU-442729. CREB phosphorylation through the activation of CaMKII.
R-MMU-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-8849932. SALM protein interactions at the synapses.

Miscellaneous databases

PROiP35438.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026959.
CleanExiMM_GRIN1.
ExpressionAtlasiP35438. baseline and differential.
GenevisibleiP35438. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR018882. CaM-bd_C0_NMDA_rcpt_NR1.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF10562. CaM_bdg_C0. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNMDZ1_MOUSE
AccessioniPrimary (citable) accession number: P35438
Secondary accession number(s): A2AI15, A2AI18, Q8CFS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 180 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.