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P35436

- NMDE1_MOUSE

UniProt

P35436 - NMDE1_MOUSE

Protein

Glutamate receptor ionotropic, NMDA 2A

Gene

Grin2a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi128 – 1281ZincBy similarity
    Metal bindingi283 – 2831ZincBy similarity
    Binding sitei518 – 5181GlutamateBy similarity
    Sitei614 – 6141Functional determinant of NMDA receptorsBy similarity
    Binding sitei731 – 7311Glutamate; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. calcium channel activity Source: MGI
    2. cation channel activity Source: MGI
    3. extracellular-glutamate-gated ion channel activity Source: RefGenome
    4. N-methyl-D-aspartate selective glutamate receptor activity Source: MGI
    5. protein binding Source: UniProtKB
    6. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. calcium ion transmembrane transport Source: GOC
    2. calcium ion transport Source: MGI
    3. directional locomotion Source: MGI
    4. dopamine metabolic process Source: MGI
    5. ionotropic glutamate receptor signaling pathway Source: GOC
    6. ion transmembrane transport Source: GOC
    7. learning Source: MGI
    8. learning or memory Source: MGI
    9. locomotion Source: MGI
    10. locomotory behavior Source: UniProtKB
    11. memory Source: MGI
    12. negative regulation of protein catabolic process Source: MGI
    13. neurogenesis Source: MGI
    14. positive regulation of apoptotic process Source: MGI
    15. protein localization Source: MGI
    16. regulation of excitatory postsynaptic membrane potential Source: MGI
    17. regulation of membrane potential Source: MGI
    18. regulation of postsynaptic membrane potential Source: MGI
    19. regulation of sensory perception of pain Source: MGI
    20. regulation of synaptic plasticity Source: UniProtKB
    21. regulation of synaptic transmission Source: UniProtKB
    22. response to amphetamine Source: MGI
    23. response to drug Source: MGI
    24. response to ethanol Source: MGI
    25. response to wounding Source: MGI
    26. sensory perception of pain Source: MGI
    27. serotonin metabolic process Source: MGI
    28. sleep Source: MGI
    29. startle response Source: MGI
    30. synaptic transmission Source: MGI
    31. synaptic transmission, glutamatergic Source: RefGenome
    32. visual learning Source: MGI

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Calcium, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_198248. CREB phosphorylation through the activation of CaMKII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate receptor ionotropic, NMDA 2A
    Short name:
    GluN2A
    Alternative name(s):
    Glutamate [NMDA] receptor subunit epsilon-1
    N-methyl D-aspartate receptor subtype 2A
    Short name:
    NMDAR2A
    Short name:
    NR2A
    Gene namesi
    Name:Grin2a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:95820. Grin2a.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cell surface Source: BHF-UCL
    3. dendritic spine Source: MGI
    4. endoplasmic reticulum Source: MGI
    5. membrane Source: MGI
    6. neuronal postsynaptic density Source: BHF-UCL
    7. neuron projection Source: BHF-UCL
    8. N-methyl-D-aspartate selective glutamate receptor complex Source: MGI
    9. postsynaptic density Source: MGI
    10. postsynaptic membrane Source: MGI
    11. presynaptic membrane Source: UniProtKB
    12. synapse Source: MGI
    13. synaptic vesicle Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 14641442Glutamate receptor ionotropic, NMDA 2APRO_0000011574Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi87 ↔ 320By similarity
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP35436.
    PRIDEiP35436.

    PTM databases

    PhosphoSiteiP35436.

    Expressioni

    Gene expression databases

    BgeeiP35436.
    GenevestigatoriP35436.

    Interactioni

    Subunit structurei

    Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 By similarity. Interacts with HIP1 and NETO1. Interacts with LRFN2 By similarity. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.By similarity5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dlg4Q621087EBI-400115,EBI-300895
    InadlQ63ZW74EBI-400115,EBI-8366894

    Protein-protein interaction databases

    BioGridi200068. 13 interactions.
    DIPiDIP-31567N.
    IntActiP35436. 6 interactions.
    MINTiMINT-103939.
    STRINGi10090.ENSMUSP00000111501.

    Structurei

    3D structure databases

    ProteinModelPortaliP35436.
    SMRiP35436. Positions 32-392, 404-841.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 555533ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini577 – 63357CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini655 – 816162ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini838 – 1464627CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei556 – 57621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei634 – 65421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei817 – 83721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni511 – 5133Glutamate bindingBy similarity
    Regioni689 – 6902Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1462 – 14643PDZ-binding

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG282132.
    GeneTreeiENSGT00540000070221.
    HOGENOMiHOG000113802.
    HOVERGENiHBG052635.
    InParanoidiP35436.
    KOiK05209.
    OMAiDYDWHVF.
    OrthoDBiEOG72ZCD1.
    PhylomeDBiP35436.
    TreeFamiTF314731.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR018884. NMDAR2_C.
    IPR028082. Peripla_BP_I.
    IPR001638. SBP_bac_3.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10565. NMDAR2_C. 1 hit.
    PF00497. SBP_bac_3. 1 hit.
    [Graphical view]
    PRINTSiPR00177. NMDARECEPTOR.
    SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35436-1 [UniParc]FASTAAdd to Basket

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    MGRLGYWTLL VLPALLVWHG PAQNAAAEKG TPALNIAVLL GHSHDVTERE     50
    LRNLWGPEQA TGLPLDVNVV ALLMNRTDPK SLITHVCDLM SGARIHGLVF 100
    GDDTDQEAVA QMLDFISSQT FIPILGIHGG ASMIMADKDP TSTFFQFGAS 150
    IQQQATVMLK IMQDYDWHVF SLVTTIFPGY RDFISFIKTT VDNSFVGWDM 200
    QNVITLDTSF EDAKTQVQLK KIHSSVILLY CSKDEAVLIL SEARSLGLTG 250
    YDFFWIVPSL VSGNTELIPK EFPSGLISVS YDDWDYSLEA RVRDGLGILT 300
    TAASSMLEKF SYIPEAKASC YGQTEKPETP LHTLHQFMVN VTWDGKDLSF 350
    TEEGYQVHPR LVVIVLNKDR EWEKVGKWEN QTLSLRHAVW PRYKSFSDCE 400
    PDDNHLSIVT LEEAPFVIVE DIDPLTETCV RNTVPCRKFV KINNSTNEGM 450
    NVKKCCKGFC IDILKKLSRT VKFTYDLYLV TNGKHGKKVN NVWNGMIGEV 500
    VYQRAVMAVG SLTINEERSE VVDFSVPFVE TGISVMVSRS NGTVSPSAFL 550
    EPFSASVWVM MFVMLLIVSA IAVFVFEYFS PVGYNRNLAK GKAPHGPSFT 600
    IGKAIWLLWG LVFNNSVPVQ NPKGTTSKIM VSVWAFFAVI FLASYTANLA 650
    AFMIQEEFVD QVTGLSDKKF QRPHDYSPPF RFGTVPNGST ERNIRNNYPY 700
    MHQYMTKFNQ RGVEDALVSL KTGKLDAFIY DAAVLNYKAG RDEGCKLVTI 750
    GSGYIFATTG YGIALQKGSP WKRQIDLALL QFVGDGEMEE LETLWLTGIC 800
    HNEKNEVMSS QLDIDNMAGV FYMLAAAMAL SLITFIWEHL FYWKLRFCFT 850
    GVCSDRPGLL FSISRGIYSC IHGVHIEEKK KSPDFNLTGS QSNMLKLLRS 900
    AKNISNMSNM NSSRMDSPKR AADFIQRGSL IVDMVSDKGN LIYSDNRSFQ 950
    GKDSIFGENM NELQTFVANR HKDSLSNYVF QGQHPLTLNE SNPNTVEVAV 1000
    STESKGNSRP RQLWKKSMES LRQDSLNQNP VSQRDEKTAE NRTHSLKSPR 1050
    YLPEEVAHSD ISETSSRATC HREPDNNKNH KTKDNFKRSM ASKYPKDCSE 1100
    VERTYVKTKA SSPRDKIYTI DGEKEPSFHL DPPQFIENIV LPENVDFPDT 1150
    YQDHNENFRK GDSTLPMNRN PLHNEDGLPN NDQYKLYAKH FTLKDKGSPH 1200
    SEGSDRYRQN STHCRSCLSN LPTYSGHFTM RSPFKCDACL RMGNLYDIDE 1250
    DQMLQETGNP ATREEAYQQD WSQNNALQFQ KNKLKINRQH SYDNILDKPR 1300
    EIDLSRPSRS ISLKDRERLL EGNLYGSLFS VPSSKLLGNK SSLFPQGLED 1350
    SKRSKSLLPD HTSDNPFLHT YGDDQRLVIG RCPSDPYKHS LPSQAVNDSY 1400
    LRSSLRSTAS YCSRDSRGHS DVYISEHVMP YAANKNNMYS TPRVLNSCSN 1450
    RRVYKKMPSI ESDV 1464
    Length:1,464
    Mass (Da):165,421
    Last modified:July 19, 2004 - v2
    Checksum:i438986DD5666C152
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10217 mRNA. Translation: BAA01069.2.
    CCDSiCCDS27943.1.
    PIRiS29159.
    RefSeqiNP_032196.2. NM_008170.2.
    XP_006521857.1. XM_006521794.1.
    UniGeneiMm.2953.

    Genome annotation databases

    EnsembliENSMUST00000032331; ENSMUSP00000032331; ENSMUSG00000059003.
    ENSMUST00000115835; ENSMUSP00000111501; ENSMUSG00000059003.
    GeneIDi14811.
    KEGGimmu:14811.
    UCSCiuc007ydc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10217 mRNA. Translation: BAA01069.2 .
    CCDSi CCDS27943.1.
    PIRi S29159.
    RefSeqi NP_032196.2. NM_008170.2.
    XP_006521857.1. XM_006521794.1.
    UniGenei Mm.2953.

    3D structure databases

    ProteinModelPortali P35436.
    SMRi P35436. Positions 32-392, 404-841.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200068. 13 interactions.
    DIPi DIP-31567N.
    IntActi P35436. 6 interactions.
    MINTi MINT-103939.
    STRINGi 10090.ENSMUSP00000111501.

    Chemistry

    BindingDBi P35436.
    GuidetoPHARMACOLOGYi 456.

    PTM databases

    PhosphoSitei P35436.

    Proteomic databases

    PaxDbi P35436.
    PRIDEi P35436.

    Protocols and materials databases

    DNASUi 14811.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032331 ; ENSMUSP00000032331 ; ENSMUSG00000059003 .
    ENSMUST00000115835 ; ENSMUSP00000111501 ; ENSMUSG00000059003 .
    GeneIDi 14811.
    KEGGi mmu:14811.
    UCSCi uc007ydc.1. mouse.

    Organism-specific databases

    CTDi 2903.
    MGIi MGI:95820. Grin2a.

    Phylogenomic databases

    eggNOGi NOG282132.
    GeneTreei ENSGT00540000070221.
    HOGENOMi HOG000113802.
    HOVERGENi HBG052635.
    InParanoidi P35436.
    KOi K05209.
    OMAi DYDWHVF.
    OrthoDBi EOG72ZCD1.
    PhylomeDBi P35436.
    TreeFami TF314731.

    Enzyme and pathway databases

    Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_198248. CREB phosphorylation through the activation of CaMKII.

    Miscellaneous databases

    NextBioi 286999.
    PROi P35436.
    SOURCEi Search...

    Gene expression databases

    Bgeei P35436.
    Genevestigatori P35436.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR018884. NMDAR2_C.
    IPR028082. Peripla_BP_I.
    IPR001638. SBP_bac_3.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10565. NMDAR2_C. 1 hit.
    PF00497. SBP_bac_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00177. NMDARECEPTOR.
    SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs."
      Meguro H., Mori H., Araki K., Kushiya E., Kutsuwada T., Yamazaki M., Kumanishi T., Arakawa M., Sakimura K., Mishina M.
      Nature 357:70-74(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Meguro H., Mori H., Araki K., Kushiya E., Kutsuwada T., Yamazaki M., Kumanishi T., Arakawa M., Sakimura K., Mishina M.
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 384.
    3. "Cloning and characterization of a novel NMDA receptor subunit NR3B: a dominant subunit that reduces calcium permeability."
      Matsuda K., Kamiya Y., Matsuda S., Yuzaki M.
      Brain Res. Mol. Brain Res. 100:43-52(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3B.
    4. "Specific assembly with the NMDA receptor 3B subunit controls surface expression and calcium permeability of NMDA receptors."
      Matsuda K., Fletcher M., Kamiya Y., Yuzaki M.
      J. Neurosci. 23:10064-10073(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3B.
    5. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1."
      Metzler M., Gan L., Wong T.P., Liu L., Helm J., Liu L., Georgiou J., Wang Y., Bissada N., Cheng K., Roder J.C., Wang Y.T., Hayden M.R.
      J. Neurosci. 27:2298-2308(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIP1.
    6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    7. "Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning."
      Ng D., Pitcher G.M., Szilard R.K., Sertie A., Kanisek M., Clapcote S.J., Lipina T., Kalia L.V., Joo D., McKerlie C., Cortez M., Roder J.C., Salter M.W., McInnes R.R.
      PLoS Biol. 7:E41-E41(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NETO1.
    8. "Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome."
      Wang X., Zhao Y., Zhang X., Badie H., Zhou Y., Mu Y., Loo L.S., Cai L., Thompson R.C., Yang B., Chen Y., Johnson P.F., Wu C., Bu G., Mobley W.C., Zhang D., Gage F.H., Ranscht B.
      , Zhang Y.W., Lipton S.A., Hong W., Xu H.
      Nat. Med. 19:473-480(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX27.

    Entry informationi

    Entry nameiNMDE1_MOUSE
    AccessioniPrimary (citable) accession number: P35436
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 147 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3