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P35436

- NMDE1_MOUSE

UniProt

P35436 - NMDE1_MOUSE

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Protein
Glutamate receptor ionotropic, NMDA 2A
Gene
Grin2a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi128 – 1281Zinc By similarity
Metal bindingi283 – 2831Zinc By similarity
Binding sitei518 – 5181Glutamate By similarity
Sitei614 – 6141Functional determinant of NMDA receptors By similarity
Binding sitei731 – 7311Glutamate; via amide nitrogen By similarity

GO - Molecular functioni

  1. N-methyl-D-aspartate selective glutamate receptor activity Source: MGI
  2. calcium channel activity Source: MGI
  3. cation channel activity Source: MGI
  4. extracellular-glutamate-gated ion channel activity Source: RefGenome
  5. protein binding Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. calcium ion transport Source: MGI
  3. directional locomotion Source: MGI
  4. dopamine metabolic process Source: MGI
  5. ion transmembrane transport Source: GOC
  6. ionotropic glutamate receptor signaling pathway Source: GOC
  7. learning Source: MGI
  8. learning or memory Source: MGI
  9. locomotion Source: MGI
  10. locomotory behavior Source: UniProtKB
  11. memory Source: MGI
  12. negative regulation of protein catabolic process Source: MGI
  13. neurogenesis Source: MGI
  14. positive regulation of apoptotic process Source: MGI
  15. protein localization Source: MGI
  16. regulation of excitatory postsynaptic membrane potential Source: MGI
  17. regulation of membrane potential Source: MGI
  18. regulation of postsynaptic membrane potential Source: MGI
  19. regulation of sensory perception of pain Source: MGI
  20. regulation of synaptic plasticity Source: UniProtKB
  21. regulation of synaptic transmission Source: UniProtKB
  22. response to amphetamine Source: MGI
  23. response to drug Source: MGI
  24. response to ethanol Source: MGI
  25. response to wounding Source: MGI
  26. sensory perception of pain Source: MGI
  27. serotonin metabolic process Source: MGI
  28. sleep Source: MGI
  29. startle response Source: MGI
  30. synaptic transmission Source: MGI
  31. synaptic transmission, glutamatergic Source: RefGenome
  32. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 2A
Short name:
GluN2A
Alternative name(s):
Glutamate [NMDA] receptor subunit epsilon-1
N-methyl D-aspartate receptor subtype 2A
Short name:
NMDAR2A
Short name:
NR2A
Gene namesi
Name:Grin2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:95820. Grin2a.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 555533Extracellular Reviewed prediction
Add
BLAST
Transmembranei556 – 57621Helical; Reviewed prediction
Add
BLAST
Topological domaini577 – 63357Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei634 – 65421Helical; Reviewed prediction
Add
BLAST
Topological domaini655 – 816162Extracellular Reviewed prediction
Add
BLAST
Transmembranei817 – 83721Helical; Reviewed prediction
Add
BLAST
Topological domaini838 – 1464627Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. N-methyl-D-aspartate selective glutamate receptor complex Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. cell surface Source: BHF-UCL
  4. dendritic spine Source: MGI
  5. endoplasmic reticulum Source: MGI
  6. membrane Source: MGI
  7. neuron projection Source: BHF-UCL
  8. neuronal postsynaptic density Source: BHF-UCL
  9. postsynaptic density Source: MGI
  10. postsynaptic membrane Source: MGI
  11. presynaptic membrane Source: UniProtKB
  12. synapse Source: MGI
  13. synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 14641442Glutamate receptor ionotropic, NMDA 2A
PRO_0000011574Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi87 ↔ 320 By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi380 – 3801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi443 – 4431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi444 – 4441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi541 – 5411N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35436.
PRIDEiP35436.

PTM databases

PhosphoSiteiP35436.

Expressioni

Gene expression databases

BgeeiP35436.
GenevestigatoriP35436.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 By similarity. Interacts with HIP1 and NETO1. Interacts with LRFN2 By similarity. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q621087EBI-400115,EBI-300895
InadlQ63ZW74EBI-400115,EBI-8366894

Protein-protein interaction databases

BioGridi200068. 13 interactions.
DIPiDIP-31567N.
IntActiP35436. 6 interactions.
MINTiMINT-103939.
STRINGi10090.ENSMUSP00000111501.

Structurei

3D structure databases

ProteinModelPortaliP35436.
SMRiP35436. Positions 32-392, 404-841.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni511 – 5133Glutamate binding By similarity
Regioni689 – 6902Glutamate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1462 – 14643PDZ-binding

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG282132.
GeneTreeiENSGT00540000070221.
HOGENOMiHOG000113802.
HOVERGENiHBG052635.
InParanoidiP35436.
KOiK05209.
OMAiDYDWHVF.
OrthoDBiEOG72ZCD1.
PhylomeDBiP35436.
TreeFamiTF314731.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR018884. NMDAR2_C.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10565. NMDAR2_C. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35436-1 [UniParc]FASTAAdd to Basket

« Hide

MGRLGYWTLL VLPALLVWHG PAQNAAAEKG TPALNIAVLL GHSHDVTERE     50
LRNLWGPEQA TGLPLDVNVV ALLMNRTDPK SLITHVCDLM SGARIHGLVF 100
GDDTDQEAVA QMLDFISSQT FIPILGIHGG ASMIMADKDP TSTFFQFGAS 150
IQQQATVMLK IMQDYDWHVF SLVTTIFPGY RDFISFIKTT VDNSFVGWDM 200
QNVITLDTSF EDAKTQVQLK KIHSSVILLY CSKDEAVLIL SEARSLGLTG 250
YDFFWIVPSL VSGNTELIPK EFPSGLISVS YDDWDYSLEA RVRDGLGILT 300
TAASSMLEKF SYIPEAKASC YGQTEKPETP LHTLHQFMVN VTWDGKDLSF 350
TEEGYQVHPR LVVIVLNKDR EWEKVGKWEN QTLSLRHAVW PRYKSFSDCE 400
PDDNHLSIVT LEEAPFVIVE DIDPLTETCV RNTVPCRKFV KINNSTNEGM 450
NVKKCCKGFC IDILKKLSRT VKFTYDLYLV TNGKHGKKVN NVWNGMIGEV 500
VYQRAVMAVG SLTINEERSE VVDFSVPFVE TGISVMVSRS NGTVSPSAFL 550
EPFSASVWVM MFVMLLIVSA IAVFVFEYFS PVGYNRNLAK GKAPHGPSFT 600
IGKAIWLLWG LVFNNSVPVQ NPKGTTSKIM VSVWAFFAVI FLASYTANLA 650
AFMIQEEFVD QVTGLSDKKF QRPHDYSPPF RFGTVPNGST ERNIRNNYPY 700
MHQYMTKFNQ RGVEDALVSL KTGKLDAFIY DAAVLNYKAG RDEGCKLVTI 750
GSGYIFATTG YGIALQKGSP WKRQIDLALL QFVGDGEMEE LETLWLTGIC 800
HNEKNEVMSS QLDIDNMAGV FYMLAAAMAL SLITFIWEHL FYWKLRFCFT 850
GVCSDRPGLL FSISRGIYSC IHGVHIEEKK KSPDFNLTGS QSNMLKLLRS 900
AKNISNMSNM NSSRMDSPKR AADFIQRGSL IVDMVSDKGN LIYSDNRSFQ 950
GKDSIFGENM NELQTFVANR HKDSLSNYVF QGQHPLTLNE SNPNTVEVAV 1000
STESKGNSRP RQLWKKSMES LRQDSLNQNP VSQRDEKTAE NRTHSLKSPR 1050
YLPEEVAHSD ISETSSRATC HREPDNNKNH KTKDNFKRSM ASKYPKDCSE 1100
VERTYVKTKA SSPRDKIYTI DGEKEPSFHL DPPQFIENIV LPENVDFPDT 1150
YQDHNENFRK GDSTLPMNRN PLHNEDGLPN NDQYKLYAKH FTLKDKGSPH 1200
SEGSDRYRQN STHCRSCLSN LPTYSGHFTM RSPFKCDACL RMGNLYDIDE 1250
DQMLQETGNP ATREEAYQQD WSQNNALQFQ KNKLKINRQH SYDNILDKPR 1300
EIDLSRPSRS ISLKDRERLL EGNLYGSLFS VPSSKLLGNK SSLFPQGLED 1350
SKRSKSLLPD HTSDNPFLHT YGDDQRLVIG RCPSDPYKHS LPSQAVNDSY 1400
LRSSLRSTAS YCSRDSRGHS DVYISEHVMP YAANKNNMYS TPRVLNSCSN 1450
RRVYKKMPSI ESDV 1464
Length:1,464
Mass (Da):165,421
Last modified:July 19, 2004 - v2
Checksum:i438986DD5666C152
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10217 mRNA. Translation: BAA01069.2.
CCDSiCCDS27943.1.
PIRiS29159.
RefSeqiNP_032196.2. NM_008170.2.
XP_006521857.1. XM_006521794.1.
UniGeneiMm.2953.

Genome annotation databases

EnsembliENSMUST00000032331; ENSMUSP00000032331; ENSMUSG00000059003.
ENSMUST00000115835; ENSMUSP00000111501; ENSMUSG00000059003.
GeneIDi14811.
KEGGimmu:14811.
UCSCiuc007ydc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10217 mRNA. Translation: BAA01069.2 .
CCDSi CCDS27943.1.
PIRi S29159.
RefSeqi NP_032196.2. NM_008170.2.
XP_006521857.1. XM_006521794.1.
UniGenei Mm.2953.

3D structure databases

ProteinModelPortali P35436.
SMRi P35436. Positions 32-392, 404-841.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200068. 13 interactions.
DIPi DIP-31567N.
IntActi P35436. 6 interactions.
MINTi MINT-103939.
STRINGi 10090.ENSMUSP00000111501.

Chemistry

BindingDBi P35436.
GuidetoPHARMACOLOGYi 456.

PTM databases

PhosphoSitei P35436.

Proteomic databases

PaxDbi P35436.
PRIDEi P35436.

Protocols and materials databases

DNASUi 14811.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032331 ; ENSMUSP00000032331 ; ENSMUSG00000059003 .
ENSMUST00000115835 ; ENSMUSP00000111501 ; ENSMUSG00000059003 .
GeneIDi 14811.
KEGGi mmu:14811.
UCSCi uc007ydc.1. mouse.

Organism-specific databases

CTDi 2903.
MGIi MGI:95820. Grin2a.

Phylogenomic databases

eggNOGi NOG282132.
GeneTreei ENSGT00540000070221.
HOGENOMi HOG000113802.
HOVERGENi HBG052635.
InParanoidi P35436.
KOi K05209.
OMAi DYDWHVF.
OrthoDBi EOG72ZCD1.
PhylomeDBi P35436.
TreeFami TF314731.

Enzyme and pathway databases

Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.

Miscellaneous databases

NextBioi 286999.
PROi P35436.
SOURCEi Search...

Gene expression databases

Bgeei P35436.
Genevestigatori P35436.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR018884. NMDAR2_C.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10565. NMDAR2_C. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs."
    Meguro H., Mori H., Araki K., Kushiya E., Kutsuwada T., Yamazaki M., Kumanishi T., Arakawa M., Sakimura K., Mishina M.
    Nature 357:70-74(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Meguro H., Mori H., Araki K., Kushiya E., Kutsuwada T., Yamazaki M., Kumanishi T., Arakawa M., Sakimura K., Mishina M.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 384.
  3. "Cloning and characterization of a novel NMDA receptor subunit NR3B: a dominant subunit that reduces calcium permeability."
    Matsuda K., Kamiya Y., Matsuda S., Yuzaki M.
    Brain Res. Mol. Brain Res. 100:43-52(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3B.
  4. "Specific assembly with the NMDA receptor 3B subunit controls surface expression and calcium permeability of NMDA receptors."
    Matsuda K., Fletcher M., Kamiya Y., Yuzaki M.
    J. Neurosci. 23:10064-10073(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3B.
  5. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1."
    Metzler M., Gan L., Wong T.P., Liu L., Helm J., Liu L., Georgiou J., Wang Y., Bissada N., Cheng K., Roder J.C., Wang Y.T., Hayden M.R.
    J. Neurosci. 27:2298-2308(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIP1.
  6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  7. "Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning."
    Ng D., Pitcher G.M., Szilard R.K., Sertie A., Kanisek M., Clapcote S.J., Lipina T., Kalia L.V., Joo D., McKerlie C., Cortez M., Roder J.C., Salter M.W., McInnes R.R.
    PLoS Biol. 7:E41-E41(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NETO1.
  8. "Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome."
    Wang X., Zhao Y., Zhang X., Badie H., Zhou Y., Mu Y., Loo L.S., Cai L., Thompson R.C., Yang B., Chen Y., Johnson P.F., Wu C., Bu G., Mobley W.C., Zhang D., Gage F.H., Ranscht B.
    , Zhang Y.W., Lipton S.A., Hong W., Xu H.
    Nat. Med. 19:473-480(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX27.

Entry informationi

Entry nameiNMDE1_MOUSE
AccessioniPrimary (citable) accession number: P35436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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