Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P35436

- NMDE1_MOUSE

UniProt

P35436 - NMDE1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamate receptor ionotropic, NMDA 2A

Gene

Grin2a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi128 – 1281ZincBy similarity
Metal bindingi283 – 2831ZincBy similarity
Binding sitei518 – 5181GlutamateBy similarity
Sitei614 – 6141Functional determinant of NMDA receptorsBy similarity
Binding sitei731 – 7311Glutamate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. calcium channel activity Source: MGI
  2. cation channel activity Source: MGI
  3. extracellular-glutamate-gated ion channel activity Source: RefGenome
  4. N-methyl-D-aspartate selective glutamate receptor activity Source: MGI
  5. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. calcium ion transport Source: MGI
  3. cation transmembrane transport Source: GOC
  4. directional locomotion Source: MGI
  5. dopamine metabolic process Source: MGI
  6. ionotropic glutamate receptor signaling pathway Source: GOC
  7. ion transmembrane transport Source: GOC
  8. learning Source: MGI
  9. learning or memory Source: MGI
  10. locomotion Source: MGI
  11. locomotory behavior Source: UniProtKB
  12. memory Source: MGI
  13. negative regulation of protein catabolic process Source: MGI
  14. neurogenesis Source: MGI
  15. positive regulation of apoptotic process Source: MGI
  16. protein localization Source: MGI
  17. regulation of excitatory postsynaptic membrane potential Source: MGI
  18. regulation of membrane potential Source: MGI
  19. regulation of postsynaptic membrane potential Source: MGI
  20. regulation of sensory perception of pain Source: MGI
  21. regulation of synaptic plasticity Source: UniProtKB
  22. regulation of synaptic transmission Source: UniProtKB
  23. response to amphetamine Source: MGI
  24. response to drug Source: MGI
  25. response to ethanol Source: MGI
  26. response to wounding Source: MGI
  27. sensory perception of pain Source: MGI
  28. serotonin metabolic process Source: MGI
  29. sleep Source: MGI
  30. startle response Source: MGI
  31. synaptic transmission Source: MGI
  32. synaptic transmission, glutamatergic Source: RefGenome
  33. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.
REACT_258217. Unblocking of NMDA receptor, glutamate binding and activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 2A
Short name:
GluN2A
Alternative name(s):
Glutamate [NMDA] receptor subunit epsilon-1
N-methyl D-aspartate receptor subtype 2A
Short name:
NMDAR2A
Short name:
NR2A
Gene namesi
Name:Grin2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:95820. Grin2a.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 555533ExtracellularSequence AnalysisAdd
BLAST
Transmembranei556 – 57621HelicalSequence AnalysisAdd
BLAST
Topological domaini577 – 63357CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei634 – 65421HelicalSequence AnalysisAdd
BLAST
Topological domaini655 – 816162ExtracellularSequence AnalysisAdd
BLAST
Transmembranei817 – 83721HelicalSequence AnalysisAdd
BLAST
Topological domaini838 – 1464627CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell surface Source: BHF-UCL
  3. dendritic spine Source: MGI
  4. endoplasmic reticulum Source: MGI
  5. membrane Source: MGI
  6. neuronal postsynaptic density Source: BHF-UCL
  7. neuron projection Source: BHF-UCL
  8. N-methyl-D-aspartate selective glutamate receptor complex Source: MGI
  9. postsynaptic density Source: MGI
  10. postsynaptic membrane Source: MGI
  11. presynaptic membrane Source: UniProtKB
  12. synapse Source: MGI
  13. synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 14641442Glutamate receptor ionotropic, NMDA 2APRO_0000011574Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi87 ↔ 320By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35436.
PRIDEiP35436.

PTM databases

PhosphoSiteiP35436.

Expressioni

Gene expression databases

BgeeiP35436.
GenevestigatoriP35436.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 (By similarity). Interacts with HIP1 and NETO1. Interacts with LRFN2 (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q621087EBI-400115,EBI-300895
InadlQ63ZW74EBI-400115,EBI-8366894

Protein-protein interaction databases

BioGridi200068. 13 interactions.
DIPiDIP-31567N.
IntActiP35436. 6 interactions.
MINTiMINT-103939.
STRINGi10090.ENSMUSP00000111501.

Structurei

3D structure databases

ProteinModelPortaliP35436.
SMRiP35436. Positions 32-842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni511 – 5133Glutamate bindingBy similarity
Regioni689 – 6902Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1462 – 14643PDZ-binding

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG282132.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000113802.
HOVERGENiHBG052635.
InParanoidiP35436.
KOiK05209.
OMAiDYDWHVF.
OrthoDBiEOG72ZCD1.
PhylomeDBiP35436.
TreeFamiTF314731.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR018884. NMDAR2_C.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10565. NMDAR2_C. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35436-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRLGYWTLL VLPALLVWHG PAQNAAAEKG TPALNIAVLL GHSHDVTERE
60 70 80 90 100
LRNLWGPEQA TGLPLDVNVV ALLMNRTDPK SLITHVCDLM SGARIHGLVF
110 120 130 140 150
GDDTDQEAVA QMLDFISSQT FIPILGIHGG ASMIMADKDP TSTFFQFGAS
160 170 180 190 200
IQQQATVMLK IMQDYDWHVF SLVTTIFPGY RDFISFIKTT VDNSFVGWDM
210 220 230 240 250
QNVITLDTSF EDAKTQVQLK KIHSSVILLY CSKDEAVLIL SEARSLGLTG
260 270 280 290 300
YDFFWIVPSL VSGNTELIPK EFPSGLISVS YDDWDYSLEA RVRDGLGILT
310 320 330 340 350
TAASSMLEKF SYIPEAKASC YGQTEKPETP LHTLHQFMVN VTWDGKDLSF
360 370 380 390 400
TEEGYQVHPR LVVIVLNKDR EWEKVGKWEN QTLSLRHAVW PRYKSFSDCE
410 420 430 440 450
PDDNHLSIVT LEEAPFVIVE DIDPLTETCV RNTVPCRKFV KINNSTNEGM
460 470 480 490 500
NVKKCCKGFC IDILKKLSRT VKFTYDLYLV TNGKHGKKVN NVWNGMIGEV
510 520 530 540 550
VYQRAVMAVG SLTINEERSE VVDFSVPFVE TGISVMVSRS NGTVSPSAFL
560 570 580 590 600
EPFSASVWVM MFVMLLIVSA IAVFVFEYFS PVGYNRNLAK GKAPHGPSFT
610 620 630 640 650
IGKAIWLLWG LVFNNSVPVQ NPKGTTSKIM VSVWAFFAVI FLASYTANLA
660 670 680 690 700
AFMIQEEFVD QVTGLSDKKF QRPHDYSPPF RFGTVPNGST ERNIRNNYPY
710 720 730 740 750
MHQYMTKFNQ RGVEDALVSL KTGKLDAFIY DAAVLNYKAG RDEGCKLVTI
760 770 780 790 800
GSGYIFATTG YGIALQKGSP WKRQIDLALL QFVGDGEMEE LETLWLTGIC
810 820 830 840 850
HNEKNEVMSS QLDIDNMAGV FYMLAAAMAL SLITFIWEHL FYWKLRFCFT
860 870 880 890 900
GVCSDRPGLL FSISRGIYSC IHGVHIEEKK KSPDFNLTGS QSNMLKLLRS
910 920 930 940 950
AKNISNMSNM NSSRMDSPKR AADFIQRGSL IVDMVSDKGN LIYSDNRSFQ
960 970 980 990 1000
GKDSIFGENM NELQTFVANR HKDSLSNYVF QGQHPLTLNE SNPNTVEVAV
1010 1020 1030 1040 1050
STESKGNSRP RQLWKKSMES LRQDSLNQNP VSQRDEKTAE NRTHSLKSPR
1060 1070 1080 1090 1100
YLPEEVAHSD ISETSSRATC HREPDNNKNH KTKDNFKRSM ASKYPKDCSE
1110 1120 1130 1140 1150
VERTYVKTKA SSPRDKIYTI DGEKEPSFHL DPPQFIENIV LPENVDFPDT
1160 1170 1180 1190 1200
YQDHNENFRK GDSTLPMNRN PLHNEDGLPN NDQYKLYAKH FTLKDKGSPH
1210 1220 1230 1240 1250
SEGSDRYRQN STHCRSCLSN LPTYSGHFTM RSPFKCDACL RMGNLYDIDE
1260 1270 1280 1290 1300
DQMLQETGNP ATREEAYQQD WSQNNALQFQ KNKLKINRQH SYDNILDKPR
1310 1320 1330 1340 1350
EIDLSRPSRS ISLKDRERLL EGNLYGSLFS VPSSKLLGNK SSLFPQGLED
1360 1370 1380 1390 1400
SKRSKSLLPD HTSDNPFLHT YGDDQRLVIG RCPSDPYKHS LPSQAVNDSY
1410 1420 1430 1440 1450
LRSSLRSTAS YCSRDSRGHS DVYISEHVMP YAANKNNMYS TPRVLNSCSN
1460
RRVYKKMPSI ESDV
Length:1,464
Mass (Da):165,421
Last modified:July 19, 2004 - v2
Checksum:i438986DD5666C152
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10217 mRNA. Translation: BAA01069.2.
CCDSiCCDS27943.1.
PIRiS29159.
RefSeqiNP_032196.2. NM_008170.2.
XP_006521857.1. XM_006521794.1.
UniGeneiMm.2953.

Genome annotation databases

EnsembliENSMUST00000032331; ENSMUSP00000032331; ENSMUSG00000059003.
ENSMUST00000115835; ENSMUSP00000111501; ENSMUSG00000059003.
GeneIDi14811.
KEGGimmu:14811.
UCSCiuc007ydc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10217 mRNA. Translation: BAA01069.2 .
CCDSi CCDS27943.1.
PIRi S29159.
RefSeqi NP_032196.2. NM_008170.2.
XP_006521857.1. XM_006521794.1.
UniGenei Mm.2953.

3D structure databases

ProteinModelPortali P35436.
SMRi P35436. Positions 32-842.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200068. 13 interactions.
DIPi DIP-31567N.
IntActi P35436. 6 interactions.
MINTi MINT-103939.
STRINGi 10090.ENSMUSP00000111501.

Chemistry

GuidetoPHARMACOLOGYi 456.

PTM databases

PhosphoSitei P35436.

Proteomic databases

PaxDbi P35436.
PRIDEi P35436.

Protocols and materials databases

DNASUi 14811.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032331 ; ENSMUSP00000032331 ; ENSMUSG00000059003 .
ENSMUST00000115835 ; ENSMUSP00000111501 ; ENSMUSG00000059003 .
GeneIDi 14811.
KEGGi mmu:14811.
UCSCi uc007ydc.1. mouse.

Organism-specific databases

CTDi 2903.
MGIi MGI:95820. Grin2a.

Phylogenomic databases

eggNOGi NOG282132.
GeneTreei ENSGT00760000119186.
HOGENOMi HOG000113802.
HOVERGENi HBG052635.
InParanoidi P35436.
KOi K05209.
OMAi DYDWHVF.
OrthoDBi EOG72ZCD1.
PhylomeDBi P35436.
TreeFami TF314731.

Enzyme and pathway databases

Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_198248. CREB phosphorylation through the activation of CaMKII.
REACT_258217. Unblocking of NMDA receptor, glutamate binding and activation.

Miscellaneous databases

NextBioi 286999.
PROi P35436.
SOURCEi Search...

Gene expression databases

Bgeei P35436.
Genevestigatori P35436.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR018884. NMDAR2_C.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10565. NMDAR2_C. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs."
    Meguro H., Mori H., Araki K., Kushiya E., Kutsuwada T., Yamazaki M., Kumanishi T., Arakawa M., Sakimura K., Mishina M.
    Nature 357:70-74(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Meguro H., Mori H., Araki K., Kushiya E., Kutsuwada T., Yamazaki M., Kumanishi T., Arakawa M., Sakimura K., Mishina M.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 384.
  3. "Cloning and characterization of a novel NMDA receptor subunit NR3B: a dominant subunit that reduces calcium permeability."
    Matsuda K., Kamiya Y., Matsuda S., Yuzaki M.
    Brain Res. Mol. Brain Res. 100:43-52(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3B.
  4. "Specific assembly with the NMDA receptor 3B subunit controls surface expression and calcium permeability of NMDA receptors."
    Matsuda K., Fletcher M., Kamiya Y., Yuzaki M.
    J. Neurosci. 23:10064-10073(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN3B.
  5. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1."
    Metzler M., Gan L., Wong T.P., Liu L., Helm J., Liu L., Georgiou J., Wang Y., Bissada N., Cheng K., Roder J.C., Wang Y.T., Hayden M.R.
    J. Neurosci. 27:2298-2308(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIP1.
  6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  7. "Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning."
    Ng D., Pitcher G.M., Szilard R.K., Sertie A., Kanisek M., Clapcote S.J., Lipina T., Kalia L.V., Joo D., McKerlie C., Cortez M., Roder J.C., Salter M.W., McInnes R.R.
    PLoS Biol. 7:E41-E41(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NETO1.
  8. "Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome."
    Wang X., Zhao Y., Zhang X., Badie H., Zhou Y., Mu Y., Loo L.S., Cai L., Thompson R.C., Yang B., Chen Y., Johnson P.F., Wu C., Bu G., Mobley W.C., Zhang D., Gage F.H., Ranscht B.
    , Zhang Y.W., Lipton S.A., Hong W., Xu H.
    Nat. Med. 19:473-480(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX27.

Entry informationi

Entry nameiNMDE1_MOUSE
AccessioniPrimary (citable) accession number: P35436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 19, 2004
Last modified: November 26, 2014
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3