P35427 (RL13A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L13a

Gene names

Name:

Rpl13a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex By similarity.
Subunit structure	Component of the 60S ribosome. Component of the GAIT complex. Interacts with EIF4G1 By similarity.
Post-translational modification	Phosphorylation at Ser-77 is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition By similarity.
Sequence similarities	Belongs to the ribosomal protein L13P family.

Ontologies

Keywords
Biological process	Translation regulation
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of formation of translation preinitiation complex Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of translation Inferred from sequence or structural similarity. Source: UniProtKB translation Inferred from electronic annotation. Source: InterPro
Cellular_component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro ribosome Non-traceable author statement Ref.1. Source: RGD
Molecular_function	structural constituent of ribosome Non-traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 203	202	60S ribosomal protein L13a		PRO_0000133773
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	77	1	Phosphoserine By similarity
	Modified residue	191	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35427 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2EEFA27AF6303727	FASTA	203	23,476
	10 20 30 40 50 60 MAEGQVLVLD GRSHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK 70 80 90 100 110 120 RMNTNPSRGP YHFRAPSRIF WRTVRGMLPH KTKRGQAALE RLKVLDGIPP PYDKKKRMVV 130 140 150 160 170 180 PAALKVVRLK PTRKFAYLGR LAHEVGWKYQ AVTATLEEKR KEKAKIHYRK KKQLLRLRKQ 190 200 AEKNVEKKIC KFTEVLKTNG LLV

References

[1]

"A leucine zipper-like motif and a basic region-leucine zipper-like element in rat ribosomal protein L13a. Identification of the tum-transplantation antigen P198." Chan Y.-L., Olvera J., Glueck A., Wool I.G. J. Biol. Chem. 269:5589-5594(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-12. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68282 mRNA. Translation: CAA48343.1.
IPI	IPI00655314.
PIR	A53204.
UniGene	Rn.92211.
3D structure databases
ProteinModelPortal	P35427.
SMR	P35427. Positions 7-142.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35427. 1 interaction.
STRING	10116.ENSRNOP00000027976.
PTM databases
PhosphoSite	P35427.
Proteomic databases
PaxDb	P35427.
PRIDE	P35427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:628697. rat.
Organism-specific databases
RGD	628697. Rpl13a.
Phylogenomic databases
eggNOG	COG0102.
HOGENOM	HOG000225289.
HOVERGEN	HBG062176.
InParanoid	P35427.
OrthoDB	EOG479F83.
Gene expression databases
ArrayExpress	P35427.
Genevestigator	P35427.
GermOnline	ENSRNOG00000020618. Rattus norvegicus.
Family and domain databases
Gene3D	3.90.1180.10. 1 hit.
InterPro	IPR005822. Ribosomal_L13. IPR023563. Ribosomal_L13_CS. IPR023564. Ribosomal_L13_dom. IPR005755. Ribosomal_L13_euk/arc. [Graphical view]
PANTHER	PTHR11545. PTHR11545. 1 hit. PTHR11545:SF3. PTHR11545:SF3. 1 hit.
Pfam	PF00572. Ribosomal_L13. 1 hit. [Graphical view]
SUPFAM	SSF52161. Ribosomal_L13. 1 hit.
TIGRFAMs	TIGR01077. L13_A_E. 1 hit.
PROSITE	PS00783. RIBOSOMAL_L13. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL13A_RAT

Accession

Primary (citable) accession number: P35427

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program