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P35423

- NSP1_ROTHD

UniProt

P35423 - NSP1_ROTHD

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Protein
Non-structural protein 1
Gene
N/A
Organism
Rotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Taxonomic identifieri10950 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001457: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Non-structural protein 1
PRO_0000149555Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8181RNA-binding By similarity
Add
BLAST
Regioni82 – 17695Important for cytoskeleton localization By similarity
Add
BLAST
Regioni313 – 486174Interaction with IRF3 By similarity
Add
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35423-1 [UniParc]FASTAAdd to Basket

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MATFKDACYQ YKKLNKLNNA VLKLGANDVW RPSTLTKRKG WCLDCCQHTD    50
LTYCQGCLIY HVCEWCSQYN RCFLDDDPHL LRMRTFRNEI TKSDLENLIN 100
MYNTLFPINK KIVHKFANTI KQHKCRNEYS TQWYNHFLMP ITLQSLSIEL 150
DGDIYYIFGY YDDMHKINQT PFSFTNLISK YDMLLLDSIN FDRMAFLPLT 200
LQQEYALRYF SKSRFITERR KCIEILHFSD NILDNLHNPN FTLQVIRNCS 250
NMSVEWNKAC NIIRNISDYF DILKSSHTEF YNISPRCRMF TQYKLKIASK 300
LIKPNYVASN HNSLATEVHN CKWCSINNNS IVWNDFRIKN VYNDIFNFIR 350
ALVKSNLYVG HCSSEEKIYE SIKEVLNVCK ENEWNMLVTE MFNQLEPIKL 400
NENNYILLNY EINWNVMNVL INSIGKIPKI LTLSDVILIL RIIIYDWFDI 450
RFMRNTPMTT FTVNKLKQLY EKDRTAEHDS RISDIE 486
Length:486
Mass (Da):57,991
Last modified:June 1, 1994 - v1
Checksum:iC802AF023F629CB5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1301S → L in ABV53253. 1 Publication
Sequence conflicti331 – 3311I → T in ABV53253. 1 Publication
Sequence conflicti374 – 3741E → D in ABV53253. 1 Publication
Sequence conflicti481 – 4811R → G in ABV53253. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L18945 Genomic RNA. Translation: AAA47332.1.
EF672578 Genomic RNA. Translation: ABV53253.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L18945 Genomic RNA. Translation: AAA47332.1 .
EF672578 Genomic RNA. Translation: ABV53253.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002148. Rotavirus_NSP1.
[Graphical view ]
Pfami PF00981. Rota_NS53. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA."
    Hua J.J., Mansell E.E., Patton J.T.
    Virology 196:372-378(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP1_ROTHD
AccessioniPrimary (citable) accession number: P35423
Secondary accession number(s): B3SRS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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