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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/United States/DS-1/1976/G2P1B[4]) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays a role in the inhibition of host innate immunity by inducing the degradation of key host factors required to activate interferon production such as IRF3, IRF5 or IRF7. Associates with components of cullin RING ligases (CRLs) including CUL1 or CUL3, which are essential multisubunit ubiquitination complexes, to modulate their activities.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1UniRule annotation
Short name:
NSP1UniRule annotation
Alternative name(s):
NCVP2UniRule annotation
Non-structural RNA-binding protein 53UniRule annotation
Short name:
NS53UniRule annotation
OrganismiRotavirus A (strain RVA/Human/United States/DS-1/1976/G2P1B[4]) (RV-A) (Rotavirus A (strain DS1))
Taxonomic identifieri10950 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001457 Componenti: Genome

Subcellular locationi

  • host cytoskeleton UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495551 – 486Non-structural protein 1Add BLAST486

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation. Interacts with host CUL1 and CUL3.UniRule annotation

Structurei

3D structure databases

SMRiP35423
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 81RNA-bindingUniRule annotationAdd BLAST81
Regioni42 – 79Zinc-binding domainUniRule annotationAdd BLAST38
Regioni82 – 176Important for cytoskeleton localizationUniRule annotationAdd BLAST95
Regioni317 – 486Interaction with host IRF3UniRule annotationAdd BLAST170

Domaini

The integrity of the zinc-binding domain in NSP1 is important for degradation of host IRF3.UniRule annotation

Sequence similaritiesi

Belongs to the rotavirus NSP1 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG09000073

Family and domain databases

HAMAPiMF_04088 ROTA_NSP1, 1 hit
InterProiView protein in InterPro
IPR002148 Rotavirus_NSP1
PfamiView protein in Pfam
PF00981 Rota_NS53, 1 hit

Sequencei

Sequence statusi: Complete.

P35423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATFKDACYQ YKKLNKLNNA VLKLGANDVW RPSTLTKRKG WCLDCCQHTD
60 70 80 90 100
LTYCQGCLIY HVCEWCSQYN RCFLDDDPHL LRMRTFRNEI TKSDLENLIN
110 120 130 140 150
MYNTLFPINK KIVHKFANTI KQHKCRNEYS TQWYNHFLMP ITLQSLSIEL
160 170 180 190 200
DGDIYYIFGY YDDMHKINQT PFSFTNLISK YDMLLLDSIN FDRMAFLPLT
210 220 230 240 250
LQQEYALRYF SKSRFITERR KCIEILHFSD NILDNLHNPN FTLQVIRNCS
260 270 280 290 300
NMSVEWNKAC NIIRNISDYF DILKSSHTEF YNISPRCRMF TQYKLKIASK
310 320 330 340 350
LIKPNYVASN HNSLATEVHN CKWCSINNNS IVWNDFRIKN VYNDIFNFIR
360 370 380 390 400
ALVKSNLYVG HCSSEEKIYE SIKEVLNVCK ENEWNMLVTE MFNQLEPIKL
410 420 430 440 450
NENNYILLNY EINWNVMNVL INSIGKIPKI LTLSDVILIL RIIIYDWFDI
460 470 480
RFMRNTPMTT FTVNKLKQLY EKDRTAEHDS RISDIE
Length:486
Mass (Da):57,991
Last modified:June 1, 1994 - v1
Checksum:iC802AF023F629CB5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130S → L in ABV53253 (PubMed:18786998).1
Sequence conflicti331I → T in ABV53253 (PubMed:18786998).1
Sequence conflicti374E → D in ABV53253 (PubMed:18786998).1
Sequence conflicti481R → G in ABV53253 (PubMed:18786998).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18945 Genomic RNA Translation: AAA47332.1
EF672578 Genomic RNA Translation: ABV53253.1

Similar proteinsi

Entry informationi

Entry nameiNSP1_ROTHD
AccessioniPrimary (citable) accession number: P35423
Secondary accession number(s): B3SRS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health