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P35423

- NSP1_ROTHD

UniProt

P35423 - NSP1_ROTHD

Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri42 – 7938Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
    3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 1
    Short name:
    NSP1
    Alternative name(s):
    NCVP2
    Non-structural RNA-binding protein 53
    Short name:
    NS53
    OrganismiRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
    Taxonomic identifieri10950 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000001457: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-KW
    2. host cytoskeleton Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 486486Non-structural protein 1PRO_0000149555Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8181RNA-bindingBy similarityAdd
    BLAST
    Regioni82 – 17695Important for cytoskeleton localizationBy similarityAdd
    BLAST
    Regioni313 – 486174Interaction with IRF3By similarityAdd
    BLAST

    Domaini

    The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.By similarity

    Sequence similaritiesi

    Belongs to the rotavirus A NSP1 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri42 – 7938Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR002148. Rotavirus_NSP1.
    [Graphical view]
    PfamiPF00981. Rota_NS53. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35423-1 [UniParc]FASTAAdd to Basket

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    MATFKDACYQ YKKLNKLNNA VLKLGANDVW RPSTLTKRKG WCLDCCQHTD    50
    LTYCQGCLIY HVCEWCSQYN RCFLDDDPHL LRMRTFRNEI TKSDLENLIN 100
    MYNTLFPINK KIVHKFANTI KQHKCRNEYS TQWYNHFLMP ITLQSLSIEL 150
    DGDIYYIFGY YDDMHKINQT PFSFTNLISK YDMLLLDSIN FDRMAFLPLT 200
    LQQEYALRYF SKSRFITERR KCIEILHFSD NILDNLHNPN FTLQVIRNCS 250
    NMSVEWNKAC NIIRNISDYF DILKSSHTEF YNISPRCRMF TQYKLKIASK 300
    LIKPNYVASN HNSLATEVHN CKWCSINNNS IVWNDFRIKN VYNDIFNFIR 350
    ALVKSNLYVG HCSSEEKIYE SIKEVLNVCK ENEWNMLVTE MFNQLEPIKL 400
    NENNYILLNY EINWNVMNVL INSIGKIPKI LTLSDVILIL RIIIYDWFDI 450
    RFMRNTPMTT FTVNKLKQLY EKDRTAEHDS RISDIE 486
    Length:486
    Mass (Da):57,991
    Last modified:June 1, 1994 - v1
    Checksum:iC802AF023F629CB5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti130 – 1301S → L in ABV53253. (PubMed:18786998)Curated
    Sequence conflicti331 – 3311I → T in ABV53253. (PubMed:18786998)Curated
    Sequence conflicti374 – 3741E → D in ABV53253. (PubMed:18786998)Curated
    Sequence conflicti481 – 4811R → G in ABV53253. (PubMed:18786998)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L18945 Genomic RNA. Translation: AAA47332.1.
    EF672578 Genomic RNA. Translation: ABV53253.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L18945 Genomic RNA. Translation: AAA47332.1 .
    EF672578 Genomic RNA. Translation: ABV53253.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002148. Rotavirus_NSP1.
    [Graphical view ]
    Pfami PF00981. Rota_NS53. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA."
      Hua J.J., Mansell E.E., Patton J.T.
      Virology 196:372-378(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
      Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
      J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNSP1_ROTHD
    AccessioniPrimary (citable) accession number: P35423
    Secondary accession number(s): B3SRS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3