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P35423 (NSP1_ROTHD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1)) [Complete proteome]
Taxonomic identifier10950 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Non-structural protein 1
PRO_0000149555

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17695Important for cytoskeleton localization By similarity
Region313 – 486174Interaction with IRF3 By similarity

Experimental info

Sequence conflict1301S → L in ABV53253. Ref.2
Sequence conflict3311I → T in ABV53253. Ref.2
Sequence conflict3741E → D in ABV53253. Ref.2
Sequence conflict4811R → G in ABV53253. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35423 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: C802AF023F629CB5

FASTA48657,991
        10         20         30         40         50         60 
MATFKDACYQ YKKLNKLNNA VLKLGANDVW RPSTLTKRKG WCLDCCQHTD LTYCQGCLIY 

        70         80         90        100        110        120 
HVCEWCSQYN RCFLDDDPHL LRMRTFRNEI TKSDLENLIN MYNTLFPINK KIVHKFANTI 

       130        140        150        160        170        180 
KQHKCRNEYS TQWYNHFLMP ITLQSLSIEL DGDIYYIFGY YDDMHKINQT PFSFTNLISK 

       190        200        210        220        230        240 
YDMLLLDSIN FDRMAFLPLT LQQEYALRYF SKSRFITERR KCIEILHFSD NILDNLHNPN 

       250        260        270        280        290        300 
FTLQVIRNCS NMSVEWNKAC NIIRNISDYF DILKSSHTEF YNISPRCRMF TQYKLKIASK 

       310        320        330        340        350        360 
LIKPNYVASN HNSLATEVHN CKWCSINNNS IVWNDFRIKN VYNDIFNFIR ALVKSNLYVG 

       370        380        390        400        410        420 
HCSSEEKIYE SIKEVLNVCK ENEWNMLVTE MFNQLEPIKL NENNYILLNY EINWNVMNVL 

       430        440        450        460        470        480 
INSIGKIPKI LTLSDVILIL RIIIYDWFDI RFMRNTPMTT FTVNKLKQLY EKDRTAEHDS 


RISDIE 

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References

[1]"Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA."
Hua J.J., Mansell E.E., Patton J.T.
Virology 196:372-378(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L18945 Genomic RNA. Translation: AAA47332.1.
EF672578 Genomic RNA. Translation: ABV53253.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTHD
AccessionPrimary (citable) accession number: P35423
Secondary accession number(s): B3SRS8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families