P35421 (PUR4_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Protein adenosine-2

Gene names

Name:	ade2
ORF Names:	CG9127

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG9961	Q9VQF4	1	EBI-89279,EBI-122086

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1354	1354	Phosphoribosylformylglycinamidine synthase		PRO_0000100403
Regions
	Domain	1087 – 1337	251	Glutamine amidotransferase type-1
	Nucleotide binding	327 – 338	12	ATP Potential
Sites
	Active site	1180	1	For GATase activity By similarity
Experimental info
	Sequence conflict	558	1	I → Y in AAC46468. Ref.1
	Sequence conflict	625	1	S → F in AAC46468. Ref.1
	Sequence conflict	766	1	E → V in AAR82811. Ref.4
	Sequence conflict	910	1	D → E in AAR82811. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P35421 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 8AA167C2D2920B2C	FASTA	1,354	148,085
	10 20 30 40 50 60 MVILRYYDVQ AHSAAEEESV LRRLREEDGA VVSVRMERCY HLEYSAQAEH SLALDELLVW 70 80 90 100 110 120 LVKQPLSKGQ SLSRQPALQS TGSSQLLLEI GPRFNFSTPY STNCVNIFQN LGYSEVRRME 130 140 150 160 170 180 TSTRYLVTFG EGSKAPEAAR FVPLLGDRMT QCLYTEENTP KASFDEQLPE RQANWHFVPV 190 200 210 220 230 240 LEEGRAALER INQELGLAFN DYDLDYYHDL FAKELGRNPT TVELFDCAQS NSEHSRHWFF 250 260 270 280 290 300 RGRMVIDGVE QPKSLIRMIM DTQAHTNPNN TIKFSDNSSA MVGFDHQTIV PSSVVAPGAV 310 320 330 340 350 360 RLQSVQSDLI FTAETHNMPT AVAPFSGATT GTGGRLRDVQ GVGRGGVPIA GTAGYCVGAL 370 380 390 400 410 420 HIPGYKQPYE PLDFKYPATF APPLQVLIEA SNGASDYGNK FGEPVISGFA LSYGLNSAAD 430 440 450 460 470 480 ASQRDEYVKP IMFSGGLGTM PATMREKLPP ARGQLLAKIG GPVYRIGVGG GAASSVEIQG 490 500 510 520 530 540 SGDAELDFNA VQRGDAEMEN KLNRVVRACL DLGEQNPILA IHDQGAGGNG NVLKELVEPG 550 560 570 580 590 600 FAGAVIFSKE FQLGDPTITA LELWGAEYQE NNAILCNADQ RELLEKICRR ERCPISFVGV 610 620 630 640 650 660 VTGDGRVTLL EKPAPKDLEQ ALNASNRSEV SPFDLELKYV LGDMPKRTYD LKREQTPLKE 670 680 690 700 710 720 LSLPKGLLLD EALERVLSLV AVGSKRFLTN KVDRCVGGLI AQQQCVGPLQ APLADYALTT 730 740 750 760 770 780 VSHFSHSGIA TSIGTQPLKG LLDPAAMARM CVAEALSNLV FVKISELADV KCSGNWMWAA 790 800 810 820 830 840 KLPGEGARMF DACKELCQIL EELHIAIDGG KDSLSMAAKV GGETIKSPGT LVISTYAPCP 850 860 870 880 890 900 DVRLKVTPDL KGPGAGSKTS LLWINLENSA RLGGSALAQA YAQQGKDTPN LTRSDVLGKA 910 920 930 940 950 960 FAVTQSLLGD GLIQAGHDVS DGGLLVCVLE MAIGGLSGLR VDLSEPLAKL KNFDKSVEKL 970 980 990 1000 1010 1020 NRPELAVLFA EECGWVVEVL DTDLERVRST YEKAGVPNYY LGVTEGFGLD SRVVLKNGKS 1030 1040 1050 1060 1070 1080 ELLDQPLRVL YKKWERTSYE LEKLQANPEC AEAEYNSLEY RQAPQYRGPQ NVQAELTLKR 1090 1100 1110 1120 1130 1140 SSAPVRVAVL REEGVNSERE MMACLLRANF EVHDVTMSDL LQGTASVSQY RGLIFPGGFS 1150 1160 1170 1180 1190 1200 YADTLGSAKG WAANILHNPR LLPQFEAFKR RQDVFSLGIC NGCQLMTLIG FVGSAKSEVG 1210 1220 1230 1240 1250 1260 ADPDVALLHN KSQRFECRWA TVKIPSNRSI MLGSMKDLVL GCWVAHGEGR FAFRDEKLIS 1270 1280 1290 1300 1310 1320 HLQSEQLVTL QYVDDVGKPT ELYPLNPNGS PQGIAGLCSS DGRHLALMPH PERCSSMYQW 1330 1340 1350 PYVPSSFEVS PTQSESPWQI MFNNAYNWCV KSNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The adenosine2 gene of Drosophila melanogaster encodes a formylglycineamide ribotide amidotransferase." Tiong S.Y.K., Nash D. Genome 36:924-934(1993) [PubMed: 8270203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00683 Unassigned DNA. Translation: AAC46468.1. AE014134 Genomic DNA. Translation: AAF52329.1. AE014134 Genomic DNA. Translation: AAN10573.1. AE014134 Genomic DNA. Translation: AAN10574.1. BT011143 mRNA. Translation: AAR82811.1.
PIR	T13363.
RefSeq	NP_477212.1. NM_057864.3. NP_723146.1. NM_164675.1. NP_723147.1. NM_164676.1.
UniGene	Dm.4474.
3D structure databases
ProteinModelPortal	P35421.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-22769N.
IntAct	P35421. 1 interaction.
MINT	MINT-1579795.
STRING	P35421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0079219; FBpp0078850; FBgn0000052. FBtr0079220; FBpp0078851; FBgn0000052. FBtr0079221; FBpp0078852; FBgn0000052.
GeneID	33847.
KEGG	dme:Dmel_CG9127.
Organism-specific databases
CTD	33847.
FlyBase	FBgn0000052. ade2.
Phylogenomic databases
eggNOG	inNOG04504.
GeneTree	EMGT00050000017325.
InParanoid	P35421.
OMA	VKAVGME.
OrthoDB	EOG4J9KDQ.
PhylomeDB	P35421.
Gene expression databases
ArrayExpress	P35421.
Bgee	P35421.
GermOnline	CG9127. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	785582.

Entry information

Entry name

PUR4_DROME

Accession

Primary (citable) accession number: P35421
Secondary accession number(s): A4V099, Q6NNY9, Q9VMI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 11, 2001
Last modified:	January 25, 2012
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project