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P35419

- PERT_MOUSE

UniProt

P35419 - PERT_MOUSE

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Protein
Thyroid peroxidase
Gene
Tpo
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activityi

2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.
[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.
[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.
2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.
[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactori

Binds 1 calcium ion per heterodimer By similarity.
Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei232 – 2321Heme (covalent; via 2 links) By similarity
Active sitei233 – 2331Proton acceptor By similarity
Metal bindingi234 – 2341Calcium By similarity
Metal bindingi313 – 3131Calcium By similarity
Metal bindingi315 – 3151Calcium; via carbonyl oxygen By similarity
Metal bindingi317 – 3171Calcium By similarity
Metal bindingi319 – 3191Calcium By similarity
Sitei384 – 3841Transition state stabilizer By similarity
Binding sitei387 – 3871Heme (covalent; via 2 links) By similarity
Metal bindingi482 – 4821Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heme binding Source: InterPro
  3. iodide peroxidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. embryonic hemopoiesis Source: Ensembl
  2. hormone biosynthetic process Source: UniProtKB-KW
  3. hydrogen peroxide catabolic process Source: UniProtKB-KW
  4. thyroid hormone generation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Thyroid hormones biosynthesis

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_219697. Thyroxine biosynthesis.
UniPathwayiUPA00194.

Protein family/group databases

PeroxiBasei3345. MmTPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Thyroid peroxidase (EC:1.11.1.8)
Short name:
TPO
Gene namesi
Name:Tpo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:98813. Tpo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 834803Extracellular Reviewed prediction
Add
BLAST
Transmembranei835 – 85925Helical; Reviewed prediction
Add
BLAST
Topological domaini860 – 91455Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed prediction
Add
BLAST
Chaini32 – 914883Thyroid peroxidase
PRO_0000023663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi136 ↔ 152 By similarity
Disulfide bondi253 ↔ 263 By similarity
Disulfide bondi257 ↔ 278 By similarity
Glycosylationi271 – 2711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi299 – 2991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi334 – 3341N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi586 ↔ 643 By similarity
Glycosylationi603 – 6031N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi684 ↔ 709 By similarity
Disulfide bondi730 ↔ 770 By similarity
Disulfide bondi756 ↔ 782 By similarity
Disulfide bondi788 ↔ 802 By similarity
Disulfide bondi796 ↔ 811 By similarity
Disulfide bondi813 ↔ 826 By similarity

Post-translational modificationi

Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface By similarity.
Cleaved in its N-terminal part By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP35419.

PTM databases

PhosphoSiteiP35419.

Expressioni

Gene expression databases

BgeeiP35419.
CleanExiMM_TPO.
GenevestigatoriP35419.

Interactioni

Subunit structurei

Interacts with DUOX1, DUOX2 and CYBA By similarity.

Structurei

3D structure databases

ProteinModelPortaliP35419.
SMRiP35419. Positions 143-722, 751-827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini728 – 78356Sushi
Add
BLAST
Domaini784 – 82744EGF-like; calcium-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262194.
GeneTreeiENSGT00550000074325.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP35419.
KOiK00431.
OMAiIMETSIQ.
OrthoDBiEOG7D2FD6.
PhylomeDBiP35419.
TreeFamiTF314316.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR000436. Sushi_SCR_CCP.
IPR029589. TPO.
[Graphical view]
PANTHERiPTHR11475:SF60. PTHR11475:SF60. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SMARTiSM00032. CCP. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35419-1 [UniParc]FASTAAdd to Basket

« Hide

MRTLGAMAIM LVVMGTVIFL SFILRSRDIL CGKTMKSHVI SAVETSQLMV    50
DHAVYNTMKR NLKKREVLSP AQLLSFFKLP ESTSGAISRA AEIMETSIQV 100
MKREQSQFST DALSADILGT IANLSGCLPF MLPPRCPDTC LANKYRPITG 150
ACNNRDHPRW GASNTALARW LPPVYEDGFS QPKGWNPNFL YHGFPLPPVR 200
EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ STSTAAFWGG 250
VDCQLTCENQ NPCFPIQLPS NSSGTTACLP FYRSSAACGT GDQGALFGNL 300
SAANPRQQMN GLTSFLDAST VYGSSPGVEK QLRNWSSSAG LLRVNTLHLD 350
AGRAYLPFAT AACAPEPGTP RTNRTPCFLA GDGRASEVPA LAAVHTLWLR 400
EHNRLASAFK AINKHWSANT AYQEARKVVG ALHQIITMRD YIPKILGPDA 450
FRQYVGPYEG YNPTVNPTVS NIFSTAAFRF GHATVHPLVR RLNTDFQEHT 500
ELPRLQLRDV FFRPWRLIQE GGLDPIVRGL LARAAKLQVQ GQLMNEELTE 550
RLFVLSNVGT LDLASLNLQR GRDHGLPDYN EWREFCGLSR LETPAELNKA 600
IANRSMVNKI MDLYKHADNI DVWLGGLAEK FLPGARTGPL FACIIGKQMK 650
ALRDGDRFWW ENTNVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG 700
KFPQDFESCE DIPSMDLELW RETFPQDDKC VFPEEVDNGN FVHCEESGKL 750
VLVYSCFHGY KLQGQEQVTC TQKGWDSEPP VCKDVNECAD LTHPPCHPSA 800
QCKNTKGSFQ CVCTDPYVLG EDEKTCIDSG RLPRASWVSI ALGALLIGGL 850
ASLTWIVICR WTHADKKATL PITERVTTQS GCRKSQGRGI SPHKAAAQDT 900
GQEPASGSRV LLCE 914
Length:914
Mass (Da):101,342
Last modified:June 1, 1994 - v1
Checksum:i595E9A0B71F3DD01
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti614 – 6141Y → H in BAC33171. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60703 mRNA. Translation: CAA43114.1.
AK047843 mRNA. Translation: BAC33171.1.
CCDSiCCDS25857.1.
PIRiJN0550.
RefSeqiNP_033443.1. NM_009417.2.
UniGeneiMm.4991.

Genome annotation databases

EnsembliENSMUST00000021005; ENSMUSP00000021005; ENSMUSG00000020673.
GeneIDi22018.
KEGGimmu:22018.
UCSCiuc007ngo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60703 mRNA. Translation: CAA43114.1 .
AK047843 mRNA. Translation: BAC33171.1 .
CCDSi CCDS25857.1.
PIRi JN0550.
RefSeqi NP_033443.1. NM_009417.2.
UniGenei Mm.4991.

3D structure databases

ProteinModelPortali P35419.
SMRi P35419. Positions 143-722, 751-827.
ModBasei Search...

Protein family/group databases

PeroxiBasei 3345. MmTPO.

PTM databases

PhosphoSitei P35419.

Proteomic databases

PRIDEi P35419.

Protocols and materials databases

DNASUi 22018.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021005 ; ENSMUSP00000021005 ; ENSMUSG00000020673 .
GeneIDi 22018.
KEGGi mmu:22018.
UCSCi uc007ngo.1. mouse.

Organism-specific databases

CTDi 7173.
MGIi MGI:98813. Tpo.

Phylogenomic databases

eggNOGi NOG262194.
GeneTreei ENSGT00550000074325.
HOGENOMi HOG000016084.
HOVERGENi HBG000071.
InParanoidi P35419.
KOi K00431.
OMAi IMETSIQ.
OrthoDBi EOG7D2FD6.
PhylomeDBi P35419.
TreeFami TF314316.

Enzyme and pathway databases

UniPathwayi UPA00194 .
Reactomei REACT_219697. Thyroxine biosynthesis.

Miscellaneous databases

NextBioi 301744.
PROi P35419.
SOURCEi Search...

Gene expression databases

Bgeei P35419.
CleanExi MM_TPO.
Genevestigatori P35419.

Family and domain databases

Gene3Di 1.10.640.10. 2 hits.
InterProi IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR000436. Sushi_SCR_CCP.
IPR029589. TPO.
[Graphical view ]
PANTHERi PTHR11475:SF60. PTHR11475:SF60. 1 hit.
Pfami PF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view ]
PRINTSi PR00457. ANPEROXIDASE.
SMARTi SM00032. CCP. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view ]
SUPFAMi SSF48113. SSF48113. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the cDNA encoding mouse thyroid peroxidase."
    Kotani T., Umeki K., Yamamoto I., Takeuchi M., Takechi S., Nakayama T., Ohtaki S.
    Gene 123:289-290(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Thyroid.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.

Entry informationi

Entry nameiPERT_MOUSE
AccessioniPrimary (citable) accession number: P35419
Secondary accession number(s): Q8C8B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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