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Protein

Thyroid peroxidase

Gene

Tpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.By similarity

Catalytic activityi

2 iodide + H2O2 + 2 H+ = diiodine + 2 H2O.By similarity
[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.By similarity
[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.By similarity
2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.By similarity
[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+PROSITE-ProRule annotationNote: Binds 1 Ca2+ ion per heterodimer.PROSITE-ProRule annotation
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per heterodimer.PROSITE-ProRule annotation

Pathwayi: thyroid hormone biosynthesis

This protein is involved in the pathway thyroid hormone biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thyroid hormone biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232Heme (covalent; via 2 links)By similarity1
Active sitei233Proton acceptorPROSITE-ProRule annotation1
Metal bindingi234CalciumPROSITE-ProRule annotation1
Metal bindingi313CalciumPROSITE-ProRule annotation1
Metal bindingi315Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi317CalciumPROSITE-ProRule annotation1
Metal bindingi319CalciumPROSITE-ProRule annotation1
Sitei384Transition state stabilizerPROSITE-ProRule annotation1
Binding sitei387Heme (covalent; via 2 links)By similarity1
Metal bindingi482Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide, Thyroid hormones biosynthesis
LigandCalcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-209968 Thyroxine biosynthesis
UniPathwayiUPA00194

Protein family/group databases

PeroxiBasei3345 MmTPO

Names & Taxonomyi

Protein namesi
Recommended name:
Thyroid peroxidase (EC:1.11.1.8By similarity)
Short name:
TPO
Gene namesi
Name:Tpo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:98813 Tpo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 834ExtracellularSequence analysisAdd BLAST803
Transmembranei835 – 859HelicalSequence analysisAdd BLAST25
Topological domaini860 – 914CytoplasmicSequence analysisAdd BLAST55

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000002366332 – 914Thyroid peroxidaseAdd BLAST883

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi123N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi136 ↔ 152By similarity
Disulfide bondi253 ↔ 263By similarity
Disulfide bondi257 ↔ 278By similarity
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi299N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi586 ↔ 643By similarity
Glycosylationi603N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi684 ↔ 709By similarity
Disulfide bondi730 ↔ 770By similarity
Disulfide bondi756 ↔ 782By similarity
Disulfide bondi788 ↔ 802By similarity
Disulfide bondi796 ↔ 811By similarity
Disulfide bondi813 ↔ 826By similarity

Post-translational modificationi

Heme is covalently bound through a H2O2-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface (By similarity).By similarity
Cleaved in its N-terminal part.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP35419
PRIDEiP35419

PTM databases

iPTMnetiP35419
PhosphoSitePlusiP35419

Expressioni

Gene expression databases

BgeeiENSMUSG00000020673
CleanExiMM_TPO
ExpressionAtlasiP35419 baseline and differential
GenevisibleiP35419 MM

Interactioni

Subunit structurei

Interacts with DUOX1, DUOX2 and CYBA.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021005

Structurei

3D structure databases

ProteinModelPortaliP35419
SMRiP35419
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini728 – 783SushiPROSITE-ProRule annotationAdd BLAST56
Domaini784 – 827EGF-like; calcium-bindingPROSITE-ProRule annotationAdd BLAST44

Sequence similaritiesi

Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2408 Eukaryota
ENOG410XPZ3 LUCA
GeneTreeiENSGT00550000074325
HOGENOMiHOG000016084
HOVERGENiHBG000071
InParanoidiP35419
KOiK00431
OMAiTCVDSGR
OrthoDBiEOG091G0236
PhylomeDBiP35419
TreeFamiTF314316

Family and domain databases

CDDicd00033 CCP, 1 hit
Gene3Di1.10.640.10, 1 hit
InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR010255 Haem_peroxidase
IPR019791 Haem_peroxidase_animal
IPR037120 Haem_peroxidase_sf
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR029589 TPO
PANTHERiPTHR11475:SF60 PTHR11475:SF60, 1 hit
PfamiView protein in Pfam
PF03098 An_peroxidase, 1 hit
PF07645 EGF_CA, 1 hit
PF00084 Sushi, 1 hit
PRINTSiPR00457 ANPEROXIDASE
SMARTiView protein in SMART
SM00032 CCP, 1 hit
SM00181 EGF, 1 hit
SM00179 EGF_CA, 1 hit
SUPFAMiSSF48113 SSF48113, 1 hit
SSF57535 SSF57535, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS01187 EGF_CA, 1 hit
PS00435 PEROXIDASE_1, 1 hit
PS50292 PEROXIDASE_3, 1 hit
PS50923 SUSHI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLGAMAIM LVVMGTVIFL SFILRSRDIL CGKTMKSHVI SAVETSQLMV
60 70 80 90 100
DHAVYNTMKR NLKKREVLSP AQLLSFFKLP ESTSGAISRA AEIMETSIQV
110 120 130 140 150
MKREQSQFST DALSADILGT IANLSGCLPF MLPPRCPDTC LANKYRPITG
160 170 180 190 200
ACNNRDHPRW GASNTALARW LPPVYEDGFS QPKGWNPNFL YHGFPLPPVR
210 220 230 240 250
EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ STSTAAFWGG
260 270 280 290 300
VDCQLTCENQ NPCFPIQLPS NSSGTTACLP FYRSSAACGT GDQGALFGNL
310 320 330 340 350
SAANPRQQMN GLTSFLDAST VYGSSPGVEK QLRNWSSSAG LLRVNTLHLD
360 370 380 390 400
AGRAYLPFAT AACAPEPGTP RTNRTPCFLA GDGRASEVPA LAAVHTLWLR
410 420 430 440 450
EHNRLASAFK AINKHWSANT AYQEARKVVG ALHQIITMRD YIPKILGPDA
460 470 480 490 500
FRQYVGPYEG YNPTVNPTVS NIFSTAAFRF GHATVHPLVR RLNTDFQEHT
510 520 530 540 550
ELPRLQLRDV FFRPWRLIQE GGLDPIVRGL LARAAKLQVQ GQLMNEELTE
560 570 580 590 600
RLFVLSNVGT LDLASLNLQR GRDHGLPDYN EWREFCGLSR LETPAELNKA
610 620 630 640 650
IANRSMVNKI MDLYKHADNI DVWLGGLAEK FLPGARTGPL FACIIGKQMK
660 670 680 690 700
ALRDGDRFWW ENTNVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG
710 720 730 740 750
KFPQDFESCE DIPSMDLELW RETFPQDDKC VFPEEVDNGN FVHCEESGKL
760 770 780 790 800
VLVYSCFHGY KLQGQEQVTC TQKGWDSEPP VCKDVNECAD LTHPPCHPSA
810 820 830 840 850
QCKNTKGSFQ CVCTDPYVLG EDEKTCIDSG RLPRASWVSI ALGALLIGGL
860 870 880 890 900
ASLTWIVICR WTHADKKATL PITERVTTQS GCRKSQGRGI SPHKAAAQDT
910
GQEPASGSRV LLCE
Length:914
Mass (Da):101,342
Last modified:June 1, 1994 - v1
Checksum:i595E9A0B71F3DD01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti614Y → H in BAC33171 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60703 mRNA Translation: CAA43114.1
AK047843 mRNA Translation: BAC33171.1
CCDSiCCDS25857.1
PIRiJN0550
RefSeqiNP_033443.1, NM_009417.3
UniGeneiMm.4991

Genome annotation databases

EnsembliENSMUST00000021005; ENSMUSP00000021005; ENSMUSG00000020673
GeneIDi22018
KEGGimmu:22018
UCSCiuc007ngo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPERT_MOUSE
AccessioniPrimary (citable) accession number: P35419
Secondary accession number(s): Q8C8B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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