Reviewed, UniProtKB/Swiss-Prot P35419 (PERT_MOUSE)

Names and origin

Protein names

Recommended name:
    Thyroid peroxidase
      Short name=TPO
    EC=1.11.1.8

Gene names

Name:

Tpo

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.
Catalytic activity	2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.
Cofactor	Binds 1 calcium ion per heterodimer By similarity. Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer By similarity.
Pathway	Hormone biosynthesis; thyroid hormone biosynthesis.
Subunit structure	Interacts with DUOX1, DUOX2 and CYBA By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity.
Post-translational modification	Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface By similarity. Cleaved in its N-terminal part By similarity.
Sequence similarities	Belongs to the peroxidase family. XPO subfamily. Contains 1 EGF-like domain. Contains 1 Sushi (CCP/SCR) domain.

Ontologies

Keywords
Biological process	Hydrogen peroxide Thyroid hormones biosynthesis
Cellular component	Membrane
Domain	EGF-like domain Signal Sushi Transmembrane
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay. Source: MGI
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro iodide peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 914	883	Thyroid peroxidase		PRO_0000023663
Regions
	Topological domain	32 – 834	803	Extracellular Potential
	Transmembrane	835 – 859	25	Potential
	Topological domain	860 – 914	55	Cytoplasmic Potential
	Domain	728 – 783	56	Sushi
	Domain	784 – 827	44	EGF-like; calcium-binding Potential
Sites
	Active site	233	1	Proton acceptor By similarity
	Metal binding	234	1	Calcium By similarity
	Metal binding	313	1	Calcium By similarity
	Metal binding	315	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	317	1	Calcium By similarity
	Metal binding	319	1	Calcium By similarity
	Metal binding	482	1	Iron (heme axial ligand) By similarity
	Binding site	232	1	Heme (covalent; via 2 links) By similarity
	Binding site	387	1	Heme (covalent; via 2 links) By similarity
	Site	384	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Glycosylation	603	1	N-linked (GlcNAc...) Potential
	Disulfide bond	136 ↔ 152		By similarity
	Disulfide bond	253 ↔ 263		By similarity
	Disulfide bond	257 ↔ 278		By similarity
	Disulfide bond	586 ↔ 643		By similarity
	Disulfide bond	684 ↔ 709		By similarity
	Disulfide bond	730 ↔ 770		By similarity
	Disulfide bond	756 ↔ 782		By similarity
	Disulfide bond	788 ↔ 802		By similarity
	Disulfide bond	796 ↔ 811		By similarity
	Disulfide bond	813 ↔ 826		By similarity
Experimental info
	Sequence conflict	614	1	Y → H in BAC33171. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35419-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 595E9A0B71F3DD01	FASTA	914	101,342
	10 20 30 40 50 60 MRTLGAMAIM LVVMGTVIFL SFILRSRDIL CGKTMKSHVI SAVETSQLMV DHAVYNTMKR 70 80 90 100 110 120 NLKKREVLSP AQLLSFFKLP ESTSGAISRA AEIMETSIQV MKREQSQFST DALSADILGT 130 140 150 160 170 180 IANLSGCLPF MLPPRCPDTC LANKYRPITG ACNNRDHPRW GASNTALARW LPPVYEDGFS 190 200 210 220 230 240 QPKGWNPNFL YHGFPLPPVR EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ 250 260 270 280 290 300 STSTAAFWGG VDCQLTCENQ NPCFPIQLPS NSSGTTACLP FYRSSAACGT GDQGALFGNL 310 320 330 340 350 360 SAANPRQQMN GLTSFLDAST VYGSSPGVEK QLRNWSSSAG LLRVNTLHLD AGRAYLPFAT 370 380 390 400 410 420 AACAPEPGTP RTNRTPCFLA GDGRASEVPA LAAVHTLWLR EHNRLASAFK AINKHWSANT 430 440 450 460 470 480 AYQEARKVVG ALHQIITMRD YIPKILGPDA FRQYVGPYEG YNPTVNPTVS NIFSTAAFRF 490 500 510 520 530 540 GHATVHPLVR RLNTDFQEHT ELPRLQLRDV FFRPWRLIQE GGLDPIVRGL LARAAKLQVQ 550 560 570 580 590 600 GQLMNEELTE RLFVLSNVGT LDLASLNLQR GRDHGLPDYN EWREFCGLSR LETPAELNKA 610 620 630 640 650 660 IANRSMVNKI MDLYKHADNI DVWLGGLAEK FLPGARTGPL FACIIGKQMK ALRDGDRFWW 670 680 690 700 710 720 ENTNVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG KFPQDFESCE DIPSMDLELW 730 740 750 760 770 780 RETFPQDDKC VFPEEVDNGN FVHCEESGKL VLVYSCFHGY KLQGQEQVTC TQKGWDSEPP 790 800 810 820 830 840 VCKDVNECAD LTHPPCHPSA QCKNTKGSFQ CVCTDPYVLG EDEKTCIDSG RLPRASWVSI 850 860 870 880 890 900 ALGALLIGGL ASLTWIVICR WTHADKKATL PITERVTTQS GCRKSQGRGI SPHKAAAQDT 910 GQEPASGSRV LLCE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the cDNA encoding mouse thyroid peroxidase." Kotani T., Umeki K., Yamamoto I., Takeuchi M., Takechi S., Nakayama T., Ohtaki S. Gene 123:289-290(1993) [PubMed: 7916704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Thyroid.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60703 mRNA. Translation: CAA43114.1. AK047843 mRNA. Translation: BAC33171.1.
IPI	IPI00118312.
PIR	JN0550.
RefSeq	NP_033443.1.
UniGene	Mm.4991
3D structure databases
HSSP	HSSP built from PDB template 1D5L based on UniProtKB P05164.
ModBase	Search...
Protein family/group databases
PeroxiBase	3345. MmTPO.
Proteomic databases
PRIDE	P35419.
Genome annotation databases
Ensembl	ENSMUSG00000020673. Mus musculus. [Contig view]
GeneID	22018.
KEGG	mmu:22018.
Organism-specific databases
MGI	MGI:98813. Tpo.
Phylogenomic databases
HOGENOM	P35419.
HOVERGEN	P35419.
OMA	P35419. HPRWGAS.
Enzyme and pathway databases
BRENDA	1.11.1.8. 244.
Gene expression databases
ArrayExpress	P35419.
Bgee	P35419.
CleanEx	MM_TPO.
GermOnline	ENSMUSG00000020673. Mus musculus.
Family and domain databases
InterPro	IPR016060. Complement_control_module. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_CS. IPR000742. EGF_3. IPR001881. EGF_Ca_bd. IPR013091. EGF_Ca_bd_2. IPR018097. EGF_Ca_bd_CS. IPR002007. Haem_peroxidase_animal. IPR019791. Haem_peroxidase_animal_sg. IPR019793. Peroxidases_heam-ligand_BS. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 1 hit. G3DSA:1.10.640.10. Haem_peroxidase_animal. 1 hit.
Pfam	PF03098. An_peroxidase. 1 hit. PF07645. EGF_CA. 1 hit. PF00084. Sushi. 1 hit. [Graphical view]
PRINTS	PR00457. ANPEROXIDASE.
SMART	SM00032. CCP. 1 hit. SM00179. EGF_CA. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS01187. EGF_CA. 1 hit. PS00435. PEROXIDASE_1. 1 hit. PS50292. PEROXIDASE_3. 1 hit. PS50923. SUSHI. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301744.
SOURCE	Search...

Entry information

Entry name

PERT_MOUSE

Accession

Primary (citable) accession number: P35419
Secondary accession number(s): Q8C8B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)