P35414 (APJ_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apelin receptor

Alternative name(s):
Angiotensin receptor-like 1
G-protein coupled receptor APJ
G-protein coupled receptor HG11

Gene names

Name:	APLNR
Synonyms:	AGTRL1, APJ

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of body fluid levels Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 380

380

Apelin receptor

PRO_0000069173

Regions

Topological domain

1 – 26

26

Extracellular Potential

Transmembrane

27 – 51

25

Helical; Name=1; Potential

Topological domain

52 – 66

15

Cytoplasmic Potential

Transmembrane

67 – 91

25

Helical; Name=2; Potential

Topological domain

92 – 100

9

Extracellular Potential

Transmembrane

101 – 125

25

Helical; Name=3; Potential

Topological domain

126 – 144

19

Cytoplasmic Potential

Transmembrane

145 – 166

22

Helical; Name=4; Potential

Topological domain

167 – 200

34

Extracellular Potential

Transmembrane

201 – 221

21

Helical; Name=5; Potential

Topological domain

222 – 244

23

Cytoplasmic Potential

Transmembrane

245 – 271

27

Helical; Name=6; Potential

Topological domain

272 – 284

13

Extracellular Potential

Transmembrane

285 – 308

24

Helical; Name=7; Potential

Topological domain

309 – 380

72

Cytoplasmic Potential

Amino acid modifications

Glycosylation

15

1

N-linked (GlcNAc...) Potential

Glycosylation

175

1

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

300

1

V → I.
Corresponds to variant rs7943508 [ dbSNP | Ensembl ].

VAR_049375

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

380

Helix Strand Turn

Details...

Beta strand

9 – 12

4

Helix

13 – 16

4

Beta strand

18 – 20

3

Turn

21 – 23

3

Helix

24 – 27

4

Beta strand

29 – 31

3

Helix

32 – 37

6

Turn

38 – 44

7

Beta strand

45 – 48

4

Helix

49 – 55

7

Sequences

	Sequence		Length	Mass (Da)
	P35414 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 78DB18BEA6D2B2E4	FASTA	380	42,660
	10 20 30 40 50 60 MEEGGDFDNY YGADNQSECE YTDWKSSGAL IPAIYMLVFL LGTTGNGLVL WTVFRSSREK 70 80 90 100 110 120 RRSADIFIAS LAVADLTFVV TLPLWATYTY RDYDWPFGTF FCKLSSYLIF VNMYASVFCL 130 140 150 160 170 180 TGLSFDRYLA IVRPVANARL RLRVSGAVAT AVLWVLAALL AMPVMVLRTT GDLENTTKVQ 190 200 210 220 230 240 CYMDYSMVAT VSSEWAWEVG LGVSSTTVGF VVPFTIMLTC YFFIAQTIAG HFRKERIEGL 250 260 270 280 290 300 RKRRRLLSII VVLVVTFALC WMPYHLVKTL YMLGSLLHWP CDFDLFLMNI FPYCTCISYV 310 320 330 340 350 360 NSCLNPFLYA FFDPRFRQAC TSMLCCGQSR CAGTSHSSSG EKSASYSSGH SQGPGPNMGK 370 380 GGEQMHEKSI PYSQETLVVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11." O'Dowd B.F., Heiber M., Chan A., Heng H.H.Q., Tsui L.-C., Kennedy J.L., Shi X., Petronis A., George S.R., Nguyen T. Gene 136:355-360(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and tissue distribution of a human orphan receptor belonging to the G protein coupled receptors family." Eggerickx D., Schurmans S., Vassart G., Parmentier M. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U03642 Genomic DNA. Translation: AAA18954.1.
X89271 mRNA. Translation: CAA61546.1.
BC032688 mRNA. Translation: AAH32688.1.

IPI

IPI00018672.

PIR

I38435.

RefSeq

NP_005152.1. NM_005161.4.

UniGene

Hs.438311.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LOT	NMR	-	A	1-55	[»]
2LOU	NMR	-	A	1-55	[»]
2LOV	NMR	-	A	1-55	[»]
2LOW	NMR	-	A	1-55	[»]

ProteinModelPortal

P35414.

ModBase

Search...

Protein-protein interaction databases

IntAct

P35414. 2 interactions.

STRING

9606.ENSP00000257254.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P35414.

Polymorphism databases

DMDM

543823.

Proteomic databases

PaxDb

P35414.

PRIDE

P35414.

Protocols and materials databases

DNASU

187.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000257254; ENSP00000257254; ENSG00000134817.

GeneID

187.

KEGG

hsa:187.

UCSC

uc001njo.3. human.

Organism-specific databases

CTD

187.

GeneCards

GC11M057001.

HGNC

HGNC:339. APLNR.

HPA

CAB025842.

MIM

600052. gene.

neXtProt

NX_P35414.

PharmGKB

PA162376687.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG304842.

HOGENOM

HOG000234122.

HOVERGEN

HBG104998.

InParanoid

P35414.

KO

K04174.

OMA

NVFPYCT.

OrthoDB

EOG4J6RRF.

PhylomeDB

P35414.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P35414.

CleanEx

HS_APLNR.

Genevestigator

P35414.

GermOnline

ENSG00000134817. Homo sapiens.

Family and domain databases

InterPro

IPR020279. Apelin_receptor_C.
IPR003904. APJ_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR01416. APJRECEPTOR.
PR00237. GPCRRHODOPSN.

ProDom

PD061381. Apelin_receptor_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P35414.

ChEMBL

CHEMBL1628481.

ChiTaRS

APLNR. human.

GenomeRNAi

187.

NextBio

768.

SOURCE

Search...

Entry information

Entry name

APJ_HUMAN

Accession

Primary (citable) accession number: P35414

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.