Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

G-protein coupled receptor 3

Gene

Gpr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons (By similarity) and neurite outgrowth (By similarity). Maintains also meiotic arrest in oocytes.By similarity2 Publications

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of cAMP metabolic process Source: Ensembl
  2. positive regulation of cytosolic calcium ion concentration Source: Ensembl
  3. regulation of meiosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 3
Alternative name(s):
GPCR21
Gene namesi
Name:Gpr3
Synonyms:Gpcr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:101908. Gpr3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242ExtracellularSequence AnalysisAdd
BLAST
Transmembranei43 – 6220Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini63 – 7412CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei75 – 9824Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini99 – 11012ExtracellularSequence AnalysisAdd
BLAST
Transmembranei111 – 13222Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini133 – 15321CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei154 – 17320Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini174 – 19825ExtracellularSequence AnalysisAdd
BLAST
Transmembranei199 – 21719Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini218 – 24528CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei246 – 27227Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini273 – 2775ExtracellularSequence Analysis
Transmembranei278 – 29922Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini300 – 33031CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are fertile but display progressive reduction in litter size despite a stable age-independent alteration of the meiotic pause, characterized by premature resumption of meiosis in about one-third of antral follicles in mutant females regardless of age. Aging mutant mice had severe reduction of fertility, manifested by an increasing number of nondeveloping early embryos upon spontaneous ovulation and massive amounts of fragmented oocytes after superovulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330G-protein coupled receptor 3PRO_0000069511Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
Lipidationi313 – 3131S-palmitoyl cysteineBy similarity
Modified residuei324 – 3241PhosphoserineSequence Analysis
Modified residuei326 – 3261PhosphoserineSequence Analysis
Modified residuei328 – 3281PhosphoserineSequence Analysis

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP35413.

Expressioni

Tissue specificityi

Expressed in both the forebrain and hindbrain, with the highest level in habenula. Lower level expression in the testis. Highly expressed in regions.2 Publications

Gene expression databases

BgeeiP35413.
CleanExiMM_GPR3.
ExpressionAtlasiP35413. baseline and differential.
GenevestigatoriP35413.

Structurei

3D structure databases

ProteinModelPortaliP35413.
SMRiP35413. Positions 41-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320944.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiP35413.
KOiK04311.
OMAiLWAICCC.
OrthoDBiEOG7966H0.
PhylomeDBiP35413.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMWGAGSSMA WFSAGSGSVN VSSVDPVEEP TGPATLLPSP RAWDVVLCIS
60 70 80 90 100
GTLVSCENAL VVAIIVGTPA FRAPMFLLVG SLAVADLLAG LGLVLHFAAD
110 120 130 140 150
FCIGSPEMSL MLVGVLAMAF TASIGSLLAI TVDRYLSLYN ALTYYSETTV
160 170 180 190 200
TRTYVMLALV WVGALGLGLV PVLAWNCRDG LTTCGVVYPL SKNHLVVLAI
210 220 230 240 250
AFFMVFGIML QLYAQICRIV CRHAQQIALQ RHLLPASHYV ATRKGIATLA
260 270 280 290 300
VVLGAFAACW LPFTVYCLLG DADSPRLYTY LTLLPATYNS MINPVIYAFR
310 320 330
NQDVQKVLWA ICCCCSTSKI PFRSRSPSDV
Length:330
Mass (Da):35,453
Last modified:June 1, 1994 - v1
Checksum:i89122C57945482B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21062 mRNA. Translation: BAA04641.1.
AK140302 mRNA. Translation: BAE24325.1.
AK140464 mRNA. Translation: BAE24399.1.
AK158917 mRNA. Translation: BAE34727.1.
AL671858 Genomic DNA. Translation: CAM21563.1.
CH466552 Genomic DNA. Translation: EDL30077.1.
BC138248 mRNA. Translation: AAI38249.1.
BC138250 mRNA. Translation: AAI38251.1.
CCDSiCCDS18743.1.
PIRiS40454.
RefSeqiNP_032180.1. NM_008154.3.
UniGeneiMm.4721.

Genome annotation databases

EnsembliENSMUST00000052090; ENSMUSP00000062083; ENSMUSG00000049649.
ENSMUST00000105911; ENSMUSP00000101531; ENSMUSG00000049649.
GeneIDi14748.
KEGGimmu:14748.
UCSCiuc008vci.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21062 mRNA. Translation: BAA04641.1.
AK140302 mRNA. Translation: BAE24325.1.
AK140464 mRNA. Translation: BAE24399.1.
AK158917 mRNA. Translation: BAE34727.1.
AL671858 Genomic DNA. Translation: CAM21563.1.
CH466552 Genomic DNA. Translation: EDL30077.1.
BC138248 mRNA. Translation: AAI38249.1.
BC138250 mRNA. Translation: AAI38251.1.
CCDSiCCDS18743.1.
PIRiS40454.
RefSeqiNP_032180.1. NM_008154.3.
UniGeneiMm.4721.

3D structure databases

ProteinModelPortaliP35413.
SMRiP35413. Positions 41-314.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi83.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PRIDEiP35413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052090; ENSMUSP00000062083; ENSMUSG00000049649.
ENSMUST00000105911; ENSMUSP00000101531; ENSMUSG00000049649.
GeneIDi14748.
KEGGimmu:14748.
UCSCiuc008vci.1. mouse.

Organism-specific databases

CTDi2827.
MGIiMGI:101908. Gpr3.

Phylogenomic databases

eggNOGiNOG320944.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiP35413.
KOiK04311.
OMAiLWAICCC.
OrthoDBiEOG7966H0.
PhylomeDBiP35413.
TreeFamiTF330052.

Miscellaneous databases

NextBioi286811.
PROiP35413.
SOURCEiSearch...

Gene expression databases

BgeeiP35413.
CleanExiMM_GPR3.
ExpressionAtlasiP35413. baseline and differential.
GenevestigatoriP35413.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel putative G protein-coupled receptor (GPCR21) which is expressed predominantly in mouse central nervous system."
    Saeki Y., Ueno S., Mizuno R., Nishimura T., Fujimura H., Nagai Y., Yanagihara T.
    FEBS Lett. 336:317-322(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina, Medulla oblongata and Visual cortex.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  7. Cited for: TISSUE SPECIFICITY, FUNCTION.

Entry informationi

Entry nameiGPR3_MOUSE
AccessioniPrimary (citable) accession number: P35413
Secondary accession number(s): Q3USD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.