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P35413 (GPR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 3
Alternative name(s):
GPCR21
Gene names
Name:Gpr3
Synonyms:Gpcr3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons By similarity and neurite outgrowth By similarity. Maintains also meiotic arrest in oocytes. Ref.6 Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in both the forebrain and hindbrain, with the highest level in habenula. Lower level expression in the testis. Highly expressed in regions. Ref.1 Ref.7

Disruption phenotype

Mice are fertile but display progressive reduction in litter size despite a stable age-independent alteration of the meiotic pause, characterized by premature resumption of meiosis in about one-third of antral follicles in mutant females regardless of age. Aging mutant mice had severe reduction of fertility, manifested by an increasing number of nondeveloping early embryos upon spontaneous ovulation and massive amounts of fragmented oocytes after superovulation. Ref.6

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330G-protein coupled receptor 3
PRO_0000069511

Regions

Topological domain1 – 4242Extracellular Potential
Transmembrane43 – 6220Helical; Name=1; Potential
Topological domain63 – 7412Cytoplasmic Potential
Transmembrane75 – 9824Helical; Name=2; Potential
Topological domain99 – 11012Extracellular Potential
Transmembrane111 – 13222Helical; Name=3; Potential
Topological domain133 – 15321Cytoplasmic Potential
Transmembrane154 – 17320Helical; Name=4; Potential
Topological domain174 – 19825Extracellular Potential
Transmembrane199 – 21719Helical; Name=5; Potential
Topological domain218 – 24528Cytoplasmic Potential
Transmembrane246 – 27227Helical; Name=6; Potential
Topological domain273 – 2775Extracellular Potential
Transmembrane278 – 29922Helical; Name=7; Potential
Topological domain300 – 33031Cytoplasmic Potential

Amino acid modifications

Modified residue3241Phosphoserine Potential
Modified residue3261Phosphoserine Potential
Modified residue3281Phosphoserine Potential
Lipidation3131S-palmitoyl cysteine By similarity
Glycosylation201N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P35413 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 89122C57945482B3

FASTA33035,453
        10         20         30         40         50         60 
MMWGAGSSMA WFSAGSGSVN VSSVDPVEEP TGPATLLPSP RAWDVVLCIS GTLVSCENAL 

        70         80         90        100        110        120 
VVAIIVGTPA FRAPMFLLVG SLAVADLLAG LGLVLHFAAD FCIGSPEMSL MLVGVLAMAF 

       130        140        150        160        170        180 
TASIGSLLAI TVDRYLSLYN ALTYYSETTV TRTYVMLALV WVGALGLGLV PVLAWNCRDG 

       190        200        210        220        230        240 
LTTCGVVYPL SKNHLVVLAI AFFMVFGIML QLYAQICRIV CRHAQQIALQ RHLLPASHYV 

       250        260        270        280        290        300 
ATRKGIATLA VVLGAFAACW LPFTVYCLLG DADSPRLYTY LTLLPATYNS MINPVIYAFR 

       310        320        330 
NQDVQKVLWA ICCCCSTSKI PFRSRSPSDV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a novel putative G protein-coupled receptor (GPCR21) which is expressed predominantly in mouse central nervous system."
Saeki Y., Ueno S., Mizuno R., Nishimura T., Fujimura H., Nagai Y., Yanagihara T.
FEBS Lett. 336:317-322(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Corpora quadrigemina, Medulla oblongata and Visual cortex.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Premature ovarian aging in mice deficient for Gpr3."
Ledent C., Demeestere I., Blum D., Petermans J., Hamalainen T., Smits G., Vassart G.
Proc. Natl. Acad. Sci. U.S.A. 102:8922-8926(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[7]"GPR3 receptor, a novel actor in the emotional-like responses."
Valverde O., Celerier E., Baranyi M., Vanderhaeghen P., Maldonado R., Sperlagh B., Vassart G., Ledent C.
PLoS ONE 4:E4704-E4704(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D21062 mRNA. Translation: BAA04641.1.
AK140302 mRNA. Translation: BAE24325.1.
AK140464 mRNA. Translation: BAE24399.1.
AK158917 mRNA. Translation: BAE34727.1.
AL671858 Genomic DNA. Translation: CAM21563.1.
CH466552 Genomic DNA. Translation: EDL30077.1.
BC138248 mRNA. Translation: AAI38249.1.
BC138250 mRNA. Translation: AAI38251.1.
PIRS40454.
RefSeqNP_032180.1. NM_008154.3.
UniGeneMm.4721.

3D structure databases

ProteinModelPortalP35413.
SMRP35413. Positions 43-314.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY83.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PRIDEP35413.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052090; ENSMUSP00000062083; ENSMUSG00000049649.
ENSMUST00000105911; ENSMUSP00000101531; ENSMUSG00000049649.
GeneID14748.
KEGGmmu:14748.
UCSCuc008vci.1. mouse.

Organism-specific databases

CTD2827.
MGIMGI:101908. Gpr3.

Phylogenomic databases

eggNOGNOG320944.
GeneTreeENSGT00750000117639.
HOGENOMHOG000294075.
HOVERGENHBG079561.
InParanoidQ3USD4.
KOK04311.
OMALWAICCC.
OrthoDBEOG7966H0.
PhylomeDBP35413.
TreeFamTF330052.

Gene expression databases

BgeeP35413.
CleanExMM_GPR3.
GenevestigatorP35413.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PANTHERPTHR22750:SF9. PTHR22750:SF9. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286811.
PROP35413.
SOURCESearch...

Entry information

Entry nameGPR3_MOUSE
AccessionPrimary (citable) accession number: P35413
Secondary accession number(s): Q3USD4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries