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Protein

G-protein coupled receptor 12

Gene

Gpr12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production (By similarity). Promotes neurite outgrowth and blocks myelin inhibition in neurons.By similarity

GO - Molecular functioni

  • G-protein coupled receptor activity Source: UniProtKB-KW
  • phosphatidylcholine binding Source: MGI

GO - Biological processi

  • cellular calcium ion homeostasis Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 12
Alternative name(s):
GPCR01
Gene namesi
Name:Gpr12
Synonyms:Gpcr12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:101909. Gpr12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848ExtracellularSequence analysisAdd
BLAST
Transmembranei49 – 6921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini70 – 789CytoplasmicSequence analysis
Transmembranei79 – 9921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini100 – 11314ExtracellularSequence analysisAdd
BLAST
Transmembranei114 – 13421Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini135 – 15824CytoplasmicSequence analysisAdd
BLAST
Transmembranei159 – 17921Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini180 – 19920ExtracellularSequence analysisAdd
BLAST
Transmembranei200 – 22021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini221 – 25232CytoplasmicSequence analysisAdd
BLAST
Transmembranei253 – 27321Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini274 – 2829ExtracellularSequence analysis
Transmembranei283 – 30321Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini304 – 33431CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334G-protein coupled receptor 12PRO_0000069528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence analysis
Lipidationi317 – 3171S-palmitoyl cysteineBy similarity
Modified residuei330 – 3301PhosphoserineSequence analysis
Modified residuei332 – 3321PhosphoserineSequence analysis

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP35412.
PRIDEiP35412.

Expressioni

Tissue specificityi

Expressed predominantly in the forebrain and a lesser extent in the hindbrain. Lower expression in the liver.

Gene expression databases

BgeeiP35412.
CleanExiMM_GPR12.
ExpressionAtlasiP35412. baseline and differential.
GenevisibleiP35412. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038245.

Structurei

3D structure databases

ProteinModelPortaliP35412.
SMRiP35412. Positions 57-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiP35412.
KOiK04315.
OMAiICCENAV.
OrthoDBiEOG7966H0.
PhylomeDBiP35412.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000599. GPR12.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00650. GPR12ORPHANR.
PR00644. GPRORPHANR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35412-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEDPKVNLS GLPRDCIDAG APENISAAVP SQGSVAESEP ELVVNPWDIV
60 70 80 90 100
LCSSGTLICC ENAVVVLIIF HSPSLRAPMF LLIGSLALAD LLAGLGLIIN
110 120 130 140 150
FVFAYLLQSE ATKLVTIGLI VASFSASVCS LLAITVDRYL SLYYALTYHS
160 170 180 190 200
ERTVTFTYVM LVMLWGTSIC LGLLPVMGWN CLRDESTCSV VRPLTKNNAA
210 220 230 240 250
ILSISFLFMF ALMLQLYIQI CKIVMRHAHQ IALQHHFLAT SHYVTTRKGV
260 270 280 290 300
STLALILGTF AACWMPFTLY SLIADYTYPS IYTYATLLPA TYNSIINPVI
310 320 330
YAFRNQEIQK ALCLICCGCI PSSLSQRARS PSDV
Length:334
Mass (Da):36,589
Last modified:June 1, 1994 - v1
Checksum:i3BE0EE4371F55F5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21061 mRNA. Translation: BAA04640.1.
BC055746 mRNA. Translation: AAH55746.1.
CCDSiCCDS19871.1.
RefSeqiNP_001010941.1. NM_001010941.2.
NP_032177.1. NM_008151.3.
XP_006504869.1. XM_006504806.2.
XP_011239303.1. XM_011241001.1.
XP_011239304.1. XM_011241002.1.
UniGeneiMm.153539.
Mm.461168.

Genome annotation databases

EnsembliENSMUST00000036211; ENSMUSP00000038245; ENSMUSG00000041468.
ENSMUST00000197431; ENSMUSP00000142889; ENSMUSG00000041468.
GeneIDi14738.
KEGGimmu:14738.
UCSCiuc009ani.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21061 mRNA. Translation: BAA04640.1.
BC055746 mRNA. Translation: AAH55746.1.
CCDSiCCDS19871.1.
RefSeqiNP_001010941.1. NM_001010941.2.
NP_032177.1. NM_008151.3.
XP_006504869.1. XM_006504806.2.
XP_011239303.1. XM_011241001.1.
XP_011239304.1. XM_011241002.1.
UniGeneiMm.153539.
Mm.461168.

3D structure databases

ProteinModelPortaliP35412.
SMRiP35412. Positions 57-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038245.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP35412.
PRIDEiP35412.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036211; ENSMUSP00000038245; ENSMUSG00000041468.
ENSMUST00000197431; ENSMUSP00000142889; ENSMUSG00000041468.
GeneIDi14738.
KEGGimmu:14738.
UCSCiuc009ani.1. mouse.

Organism-specific databases

CTDi2835.
MGIiMGI:101909. Gpr12.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiP35412.
KOiK04315.
OMAiICCENAV.
OrthoDBiEOG7966H0.
PhylomeDBiP35412.
TreeFamiTF330052.

Miscellaneous databases

PROiP35412.
SOURCEiSearch...

Gene expression databases

BgeeiP35412.
CleanExiMM_GPR12.
ExpressionAtlasiP35412. baseline and differential.
GenevisibleiP35412. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000599. GPR12.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00650. GPR12ORPHANR.
PR00644. GPRORPHANR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel putative G protein-coupled receptor (GPCR21) which is expressed predominantly in mouse central nervous system."
    Saeki Y., Ueno S., Mizuno R., Nishimura T., Fujimura H., Nagai Y., Yanagihara T.
    FEBS Lett. 336:317-322(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development."
    Ignatov A., Lintzel J., Hermans-Borgmeyer I., Kreienkamp H.J., Joost P., Thomsen S., Methner A., Schaller H.C.
    J. Neurosci. 23:907-914(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY FUNCTION.

Entry informationi

Entry nameiGPR12_MOUSE
AccessioniPrimary (citable) accession number: P35412
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 8, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a receptor for sphingosine 1-phosphate.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.