P35411 (CX3C1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CX3C chemokine receptor 1
Short name=C-X3-C CKR-1
Short name=CX3CR1

Alternative name(s):
Fractalkine receptor
RBS11

Gene names

Name:	Cx3cr1
Synonyms:	Rbs11

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Most abundant in adult spinal cord, brain, kidney, gut, uterus and testes.
Post-translational modification	This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro cellular response to lipopolysaccharide Inferred from expression pattern PubMed 10422773. Source: RGD cellular response to transforming growth factor beta stimulus Inferred from expression pattern PubMed 12446007. Source: RGD cerebral cortex cell migration Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: InterPro macrophage chemotaxis Inferred from electronic annotation. Source: Compara microglial cell activation involved in immune response Inferred from electronic annotation. Source: Compara negative regulation of angiogenesis Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of cell migration Inferred from mutant phenotype PubMed 17114429. Source: RGD positive regulation of angiogenesis Inferred from mutant phenotype PubMed 18006432. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from direct assay PubMed 10869418. Source: RGD neuronal cell body membrane Inferred from direct assay PubMed 10869418. Source: RGD perinuclear region of cytoplasm Inferred from direct assay PubMed 11870871PubMed 19748551. Source: RGD
Molecular_function	C-X3-C chemokine receptor activity Inferred from direct assay PubMed 11278650. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	CX3C chemokine receptor 1		PRO_0000069328
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 60	28	Helical; Name=1; Potential
	Topological domain	61 – 70	10	Cytoplasmic Potential
	Transmembrane	71 – 91	21	Helical; Name=2; Potential
	Topological domain	92 – 104	13	Extracellular Potential
	Transmembrane	105 – 126	22	Helical; Name=3; Potential
	Topological domain	127 – 143	17	Cytoplasmic Potential
	Transmembrane	144 – 168	25	Helical; Name=4; Potential
	Topological domain	169 – 196	28	Extracellular Potential
	Transmembrane	197 – 216	20	Helical; Name=5; Potential
	Topological domain	217 – 232	16	Cytoplasmic Potential
	Transmembrane	233 – 257	25	Helical; Name=6; Potential
	Topological domain	258 – 274	17	Extracellular Potential
	Transmembrane	275 – 298	24	Helical; Name=7; Potential
	Topological domain	299 – 354	56	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	103 ↔ 176		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35411 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: CFC4578095E77376	FASTA	354	40,327
	10 20 30 40 50 60 MPTSFPELDL ENFEYDDSAE ACYLGDIVAF GTIFLSIFYS LVFTFGLVGN LLVVLALTNS 70 80 90 100 110 120 RKSKSITDIY LLNLALSDLL FVATLPFWTH YLISHEGLHN AMCKLTTAFF FIGFFGGIFF 130 140 150 160 170 180 ITVISIDRYL AIVLAANSMN NRTVQHGVTI SLGVWAAAIL VASPQFMFTK RKDNECLGDY 190 200 210 220 230 240 PEVLQEIWPV LRNSEVNILG FVLPLLIMSF CYFRIVRTLF SCKNRKKARA IRLILLVVVV 250 260 270 280 290 300 FFLFWTPYNI VIFLETLKFY NFFPSCGMKR DLRWALSVTE TVAFSHCCLN PFIYAFAGEK 310 320 330 340 350 FRRYLRHLYN KCLAVLCGRP VHAGFSTESQ RSRQDSILSS LTHYTSEGEG SLLL

References

[1]	"cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors." Harrison J.K., Barber C.M., Lynch K.R. Neurosci. Lett. 169:85-89(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04808 mRNA. Translation: AAB87093.1.
IPI	IPI00198598.
PIR	I58186.
RefSeq	NP_598218.1. NM_133534.1.
UniGene	Rn.10482.
3D structure databases
ProteinModelPortal	P35411.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000025019.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35411.
Proteomic databases
PRIDE	P35411.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025019; ENSRNOP00000025019; ENSRNOG00000018509.
GeneID	171056.
KEGG	rno:171056.
UCSC	RGD:620137. rat.
Organism-specific databases
CTD	1524.
RGD	620137. Cx3cr1.
Phylogenomic databases
eggNOG	NOG151350.
GeneTree	ENSGT00700000104251.
HOGENOM	HOG000234122.
HOVERGEN	HBG106917.
InParanoid	P35411.
KO	K04192.
OMA	APQFMFT.
OrthoDB	EOG4WM4VB.
Gene expression databases
Genevestigator	P35411.
GermOnline	ENSRNOG00000018509. Rattus norvegicus.
Family and domain databases
InterPro	IPR005387. Chemokine_CX3CR1. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01562. FRACTALKINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	621621.

Entry information

Entry name

CX3C1_RAT

Accession

Primary (citable) accession number: P35411

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program