Prostaglandin E2 receptor EP4 subtype

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Reviewed, UniProtKB/Swiss-Prot P35408 (PE2R4_HUMAN)

Last modified June 16, 2009. Version 87. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Prostaglandin E2 receptor EP4 subtype
      Short name=PGE receptor, EP4 subtype
Alternative name(s):
    Prostanoid EP4 receptor

Gene names

Name:	PTGER4
Synonyms:	PTGER2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Subunit structure	Interacts with FEM1A. Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally designated as the EP2 subtype.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cAMP nucleotide second messenger Ref.1 Traceable author statement. Source: ProtInc immune response Ref.4 Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Ref.1 Traceable author statement. Source: ProtInc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	prostaglandin E receptor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 488

488

Prostaglandin E2 receptor EP4 subtype

PRO_0000070064

Regions

Topological domain

1 – 19

Extracellular Potential

Transmembrane

20 – 43

1 Potential

Topological domain

44 – 55

Cytoplasmic Potential

Transmembrane

56 – 79

2 Potential

Topological domain

80 – 96

Extracellular Potential

Transmembrane

97 – 115

3 Potential

Topological domain

116 – 135

Cytoplasmic Potential

Transmembrane

136 – 160

4 Potential

Topological domain

161 – 184

Extracellular Potential

Transmembrane

185 – 211

5 Potential

Topological domain

212 – 267

Cytoplasmic Potential

Transmembrane

268 – 295

6 Potential

Topological domain

296 – 312

Extracellular Potential

Transmembrane

313 – 332

7 Potential

Topological domain

333 – 488

156

Cytoplasmic Potential

Compositional bias

230 – 234

Poly-Ala

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

92 ↔ 170

By similarity

Experimental info

Sequence conflict

464 – 466

GPA → WAC in AAA36438. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P35408-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D028478CD72C85EE
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FASTA

488

53,119

        10         20         30         40         50         60 
MSTPGVNSSA SLSPDRLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK ETTFYTLVCG 

        70         80         90        100        110        120 
LAVTDLLGTL LVSPVTIATY MKGQWPGGQP LCEYSTFILL FFSLSGLSII CAMSVERYLA 

       130        140        150        160        170        180 
INHAYFYSHY VDKRLAGLTL FAVYASNVLF CALPNMGLGS SRLQYPDTWC FIDWTTNVTA 

       190        200        210        220        230        240 
HAAYSYMYAG FSSFLILATV LCNVLVCGAL LRMHRQFMRR TSLGTEQHHA AAAASVASRG 

       250        260        270        280        290        300 
HPAASPALPR LSDFRRRRSF RRIAGAEIQM VILLIATSLV VLICSIPLVV RVFVNQLYQP 

       310        320        330        340        350        360 
SLEREVSKNP DLQAIRIASV NPILDPWIYI LLRKTVLSKA IEKIKCLFCR IGGSRRERSG 

       370        380        390        400        410        420 
QHCSDSQRTS SAMSGHSRSF ISRELKEISS TSQTLLPDLS LPDLSENGLG GRNLLPGVPG 

       430        440        450        460        470        480 
MGLAQEDTTS LRTLRISETS DSSQGQDSES VLLVDEAGGS GRAGPAPKGS SLQVTFPSET 


LNLSEKCI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, functional expression, and characterization of the human prostaglandin E2 receptor EP2 subtype." Bastien L., Sawyer N., Grygorczyk R., Metters K.M., Adam M. J. Biol. Chem. 269:11873-11877(1994) [PubMed: 8163486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Cloning and expression of the EP2 subtype of human receptors for prostaglandin E2." An S., Yang J., Xia M., Goetzl E.J. Biochem. Biophys. Res. Commun. 197:263-270(1993) [PubMed: 8250933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[3]	"The structure of the prostaglandin EP4 receptor gene and related pseudogenes." Foord S.M., Marks B., Stolz M., Bufflier E., Fraser N.J., Lee M.G. Genomics 35:182-188(1996) [PubMed: 8661119] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Gene expression of the human prostaglandin E receptor EP4 subtype: differential regulation in monocytoid and lymphoid lineage cells by phorbol ester." Mori K., Tanaka I., Kotani M., Miyaoka F., Sando T., Muro S., Sasaki Y., Nakagawa O., Ogawa Y., Usui T., Ozaki S., Ichikawa A., Narumiya S., Nakao K. J. Mol. Med. 74:333-336(1996) [PubMed: 8862514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	SeattleSNPs variation discovery resource Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[8]	"A novel prostaglandin E receptor 4-associated protein participates in antiinflammatory signaling." Takayama K., Sukhova G.K., Chin M.T., Libby P. Circ. Res. 98:499-504(2006) [PubMed: 16424369] [Abstract] Cited for: INTERACTION WITH FEM1A.
+	Additional computationally mapped references.

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Web resources

SeattleSNPs

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Cross-references

Sequence databases
	L28175 mRNA. Translation: AAA36434.1. L25124 mRNA. Translation: AAA36438.1. X97873, X97874 Genomic DNA. Translation: CAA66463.1. D28472 mRNA. Translation: BAA05834.1. AY429109 mRNA. Translation: AAR07904.1. DQ400918 Genomic DNA. Translation: ABD48960.1. BC101534 mRNA. Translation: AAI01535.1. BC113523 mRNA. Translation: AAI13524.1.
IPI	IPI00018648.
PIR	A53572.
RefSeq	NP_000949.1.
UniGene	Hs.199248
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35408.
Proteomic databases
PRIDE	P35408.
Genome annotation databases
Ensembl	ENSG00000171522. Homo sapiens. [Contig view]
GeneID	5734.
KEGG	hsa:5734.
Organism-specific databases
GeneCards	GC05P040715.
H-InvDB	HIX0032033.
HGNC	HGNC:9596. PTGER4.
HPA	HPA011226. HPA012756.
MIM	601586. gene.
PharmGKB	PA289.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P35408.
HOVERGEN	P35408.
OMA	P35408. AIEKIKC.
Gene expression databases
ArrayExpress	P35408.
Bgee	P35408.
CleanEx	HS_PTGER2. HS_PTGER4.
GermOnline	ENSG00000171522. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001758. Prost_EP4_rcpt. IPR008365. Prostanoid_rcpt. IPR001244. Prostglndn_DP_rcpt. [Graphical view]
PANTHER	PTHR11866:SF6. Prost_EP4_rcpt. 1 hit. PTHR11866. Prostanoid_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00428. PROSTAGLNDNR. PR01788. PROSTANOIDR. PR00586. PRSTNOIDEP4R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	22322.
SOURCE	Search...

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Entry information

Entry name

PE2R4_HUMAN

Accession

Primary (citable) accession number: P35408
Secondary accession number(s): Q3MJ87

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents