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Protein

Prostaglandin E2 receptor EP4 subtype

Gene

PTGER4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

GO - Molecular functioni

  • prostaglandin E receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171522-MONOMER.
ReactomeiR-HSA-391908. Prostanoid ligand receptors.
R-HSA-418555. G alpha (s) signalling events.
SIGNORiP35408.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name:
PGE receptor EP4 subtype
Short name:
PGE2 receptor EP4 subtype
Alternative name(s):
Prostanoid EP4 receptor
Gene namesi
Name:PTGER4
Synonyms:PTGER2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:9596. PTGER4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19ExtracellularSequence analysisAdd BLAST19
Transmembranei20 – 43Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini44 – 55CytoplasmicSequence analysisAdd BLAST12
Transmembranei56 – 79Helical; Name=2Sequence analysisAdd BLAST24
Topological domaini80 – 96ExtracellularSequence analysisAdd BLAST17
Transmembranei97 – 115Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini116 – 135CytoplasmicSequence analysisAdd BLAST20
Transmembranei136 – 160Helical; Name=4Sequence analysisAdd BLAST25
Topological domaini161 – 184ExtracellularSequence analysisAdd BLAST24
Transmembranei185 – 211Helical; Name=5Sequence analysisAdd BLAST27
Topological domaini212 – 267CytoplasmicSequence analysisAdd BLAST56
Transmembranei268 – 295Helical; Name=6Sequence analysisAdd BLAST28
Topological domaini296 – 312ExtracellularSequence analysisAdd BLAST17
Transmembranei313 – 332Helical; Name=7Sequence analysisAdd BLAST20
Topological domaini333 – 488CytoplasmicSequence analysisAdd BLAST156

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5734.
OpenTargetsiENSG00000171522.
PharmGKBiPA289.

Chemistry databases

ChEMBLiCHEMBL1836.
DrugBankiDB00917. Dinoprostone.
DB00929. Misoprostol.
GuidetoPHARMACOLOGYi343.

Polymorphism and mutation databases

BioMutaiPTGER4.
DMDMi548476.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700641 – 488Prostaglandin E2 receptor EP4 subtypeAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi7N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi92 ↔ 170PROSITE-ProRule annotation
Modified residuei374Phosphoserine1 Publication1
Modified residuei377Phosphoserine1 Publication1
Modified residuei379Phosphoserine1 Publication1
Modified residuei382Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP35408.
PaxDbiP35408.
PeptideAtlasiP35408.
PRIDEiP35408.

PTM databases

iPTMnetiP35408.
PhosphoSitePlusiP35408.

Expressioni

Tissue specificityi

High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Gene expression databases

BgeeiENSG00000171522.
CleanExiHS_PTGER2.
HS_PTGER4.
GenevisibleiP35408. HS.

Organism-specific databases

HPAiHPA011226.
HPA012756.

Interactioni

Subunit structurei

Interacts with FEM1A.1 Publication

Protein-protein interaction databases

BioGridi111706. 3 interactors.
DIPiDIP-61099N.
STRINGi9606.ENSP00000302846.

Chemistry databases

BindingDBiP35408.

Structurei

3D structure databases

ProteinModelPortaliP35408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi230 – 234Poly-Ala5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119188.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP35408.
KOiK04261.
OMAiIDWRTNV.
OrthoDBiEOG091G060C.
PhylomeDBiP35408.
TreeFamiTF324982.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 2 hits.
PTHR11866:SF6. PTHR11866:SF6. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPGVNSSA SLSPDRLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK
60 70 80 90 100
ETTFYTLVCG LAVTDLLGTL LVSPVTIATY MKGQWPGGQP LCEYSTFILL
110 120 130 140 150
FFSLSGLSII CAMSVERYLA INHAYFYSHY VDKRLAGLTL FAVYASNVLF
160 170 180 190 200
CALPNMGLGS SRLQYPDTWC FIDWTTNVTA HAAYSYMYAG FSSFLILATV
210 220 230 240 250
LCNVLVCGAL LRMHRQFMRR TSLGTEQHHA AAAASVASRG HPAASPALPR
260 270 280 290 300
LSDFRRRRSF RRIAGAEIQM VILLIATSLV VLICSIPLVV RVFVNQLYQP
310 320 330 340 350
SLEREVSKNP DLQAIRIASV NPILDPWIYI LLRKTVLSKA IEKIKCLFCR
360 370 380 390 400
IGGSRRERSG QHCSDSQRTS SAMSGHSRSF ISRELKEISS TSQTLLPDLS
410 420 430 440 450
LPDLSENGLG GRNLLPGVPG MGLAQEDTTS LRTLRISETS DSSQGQDSES
460 470 480
VLLVDEAGGS GRAGPAPKGS SLQVTFPSET LNLSEKCI
Length:488
Mass (Da):53,119
Last modified:June 1, 1994 - v1
Checksum:iD028478CD72C85EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464 – 466GPA → WAC in AAA36438 (PubMed:8250933).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28175 mRNA. Translation: AAA36434.1.
L25124 mRNA. Translation: AAA36438.1.
X97873, X97874 Genomic DNA. Translation: CAA66463.1.
D28472 mRNA. Translation: BAA05834.1.
AY429109 mRNA. Translation: AAR07904.1.
DQ400918 Genomic DNA. Translation: ABD48960.1.
BC101534 mRNA. Translation: AAI01535.1.
BC113523 mRNA. Translation: AAI13524.1.
CCDSiCCDS3930.1.
PIRiA53572.
RefSeqiNP_000949.1. NM_000958.2.
UniGeneiHs.199248.

Genome annotation databases

EnsembliENST00000302472; ENSP00000302846; ENSG00000171522.
GeneIDi5734.
KEGGihsa:5734.
UCSCiuc003jlz.5. human.

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28175 mRNA. Translation: AAA36434.1.
L25124 mRNA. Translation: AAA36438.1.
X97873, X97874 Genomic DNA. Translation: CAA66463.1.
D28472 mRNA. Translation: BAA05834.1.
AY429109 mRNA. Translation: AAR07904.1.
DQ400918 Genomic DNA. Translation: ABD48960.1.
BC101534 mRNA. Translation: AAI01535.1.
BC113523 mRNA. Translation: AAI13524.1.
CCDSiCCDS3930.1.
PIRiA53572.
RefSeqiNP_000949.1. NM_000958.2.
UniGeneiHs.199248.

3D structure databases

ProteinModelPortaliP35408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111706. 3 interactors.
DIPiDIP-61099N.
STRINGi9606.ENSP00000302846.

Chemistry databases

BindingDBiP35408.
ChEMBLiCHEMBL1836.
DrugBankiDB00917. Dinoprostone.
DB00929. Misoprostol.
GuidetoPHARMACOLOGYi343.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP35408.
PhosphoSitePlusiP35408.

Polymorphism and mutation databases

BioMutaiPTGER4.
DMDMi548476.

Proteomic databases

MaxQBiP35408.
PaxDbiP35408.
PeptideAtlasiP35408.
PRIDEiP35408.

Protocols and materials databases

DNASUi5734.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302472; ENSP00000302846; ENSG00000171522.
GeneIDi5734.
KEGGihsa:5734.
UCSCiuc003jlz.5. human.

Organism-specific databases

CTDi5734.
DisGeNETi5734.
GeneCardsiPTGER4.
H-InvDBHIX0032033.
HGNCiHGNC:9596. PTGER4.
HPAiHPA011226.
HPA012756.
MIMi601586. gene.
neXtProtiNX_P35408.
OpenTargetsiENSG00000171522.
PharmGKBiPA289.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119188.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP35408.
KOiK04261.
OMAiIDWRTNV.
OrthoDBiEOG091G060C.
PhylomeDBiP35408.
TreeFamiTF324982.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171522-MONOMER.
ReactomeiR-HSA-391908. Prostanoid ligand receptors.
R-HSA-418555. G alpha (s) signalling events.
SIGNORiP35408.

Miscellaneous databases

GeneWikiiEP4_receptor.
GenomeRNAii5734.
PROiP35408.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171522.
CleanExiHS_PTGER2.
HS_PTGER4.
GenevisibleiP35408. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 2 hits.
PTHR11866:SF6. PTHR11866:SF6. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPE2R4_HUMAN
AccessioniPrimary (citable) accession number: P35408
Secondary accession number(s): Q3MJ87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally designated as the EP2 subtype.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.