P35408 (PE2R4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name=PGE receptor EP4 subtype
Short name=PGE2 receptor EP4 subtype

Alternative name(s):
Prostanoid EP4 receptor

Gene names

Name:	PTGER4
Synonyms:	PTGER2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Subunit structure	Interacts with FEM1A. Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally designated as the EP2 subtype.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cAMP nucleotide second messenger Traceable author statement. Source: ProtInc immune response Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Traceable author statement. Source: ProtInc plasma membrane Traceable author statement. Source: Reactome
Molecular function	prostaglandin E receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Prostaglandin E2 receptor EP4 subtype		PRO_0000070064
Regions
	Topological domain	1 – 19	19	Extracellular Potential
	Transmembrane	20 – 43	24	Helical; Name=1; Potential
	Topological domain	44 – 55	12	Cytoplasmic Potential
	Transmembrane	56 – 79	24	Helical; Name=2; Potential
	Topological domain	80 – 96	17	Extracellular Potential
	Transmembrane	97 – 115	19	Helical; Name=3; Potential
	Topological domain	116 – 135	20	Cytoplasmic Potential
	Transmembrane	136 – 160	25	Helical; Name=4; Potential
	Topological domain	161 – 184	24	Extracellular Potential
	Transmembrane	185 – 211	27	Helical; Name=5; Potential
	Topological domain	212 – 267	56	Cytoplasmic Potential
	Transmembrane	268 – 295	28	Helical; Name=6; Potential
	Topological domain	296 – 312	17	Extracellular Potential
	Transmembrane	313 – 332	20	Helical; Name=7; Potential
	Topological domain	333 – 488	156	Cytoplasmic Potential
	Compositional bias	230 – 234	5	Poly-Ala
Amino acid modifications
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Disulfide bond	92 ↔ 170		By similarity
Experimental info
	Sequence conflict	464 – 466	3	GPA → WAC in AAA36438. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35408 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D028478CD72C85EE	FASTA	488	53,119
	10 20 30 40 50 60 MSTPGVNSSA SLSPDRLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK ETTFYTLVCG 70 80 90 100 110 120 LAVTDLLGTL LVSPVTIATY MKGQWPGGQP LCEYSTFILL FFSLSGLSII CAMSVERYLA 130 140 150 160 170 180 INHAYFYSHY VDKRLAGLTL FAVYASNVLF CALPNMGLGS SRLQYPDTWC FIDWTTNVTA 190 200 210 220 230 240 HAAYSYMYAG FSSFLILATV LCNVLVCGAL LRMHRQFMRR TSLGTEQHHA AAAASVASRG 250 260 270 280 290 300 HPAASPALPR LSDFRRRRSF RRIAGAEIQM VILLIATSLV VLICSIPLVV RVFVNQLYQP 310 320 330 340 350 360 SLEREVSKNP DLQAIRIASV NPILDPWIYI LLRKTVLSKA IEKIKCLFCR IGGSRRERSG 370 380 390 400 410 420 QHCSDSQRTS SAMSGHSRSF ISRELKEISS TSQTLLPDLS LPDLSENGLG GRNLLPGVPG 430 440 450 460 470 480 MGLAQEDTTS LRTLRISETS DSSQGQDSES VLLVDEAGGS GRAGPAPKGS SLQVTFPSET LNLSEKCI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, functional expression, and characterization of the human prostaglandin E2 receptor EP2 subtype." Bastien L., Sawyer N., Grygorczyk R., Metters K.M., Adam M. J. Biol. Chem. 269:11873-11877(1994) [PubMed: 8163486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Cloning and expression of the EP2 subtype of human receptors for prostaglandin E2." An S., Yang J., Xia M., Goetzl E.J. Biochem. Biophys. Res. Commun. 197:263-270(1993) [PubMed: 8250933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[3]	"The structure of the prostaglandin EP4 receptor gene and related pseudogenes." Foord S.M., Marks B., Stolz M., Bufflier E., Fraser N.J., Lee M.G. Genomics 35:182-188(1996) [PubMed: 8661119] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Gene expression of the human prostaglandin E receptor EP4 subtype: differential regulation in monocytoid and lymphoid lineage cells by phorbol ester." Mori K., Tanaka I., Kotani M., Miyaoka F., Sando T., Muro S., Sasaki Y., Nakagawa O., Ogawa Y., Usui T., Ozaki S., Ichikawa A., Narumiya S., Nakao K. J. Mol. Med. 74:333-336(1996) [PubMed: 8862514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	SeattleSNPs variation discovery resource Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[8]	"A novel prostaglandin E receptor 4-associated protein participates in antiinflammatory signaling." Takayama K., Sukhova G.K., Chin M.T., Libby P. Circ. Res. 98:499-504(2006) [PubMed: 16424369] [Abstract] Cited for: INTERACTION WITH FEM1A.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	L28175 mRNA. Translation: AAA36434.1. L25124 mRNA. Translation: AAA36438.1. X97873, X97874 Genomic DNA. Translation: CAA66463.1. D28472 mRNA. Translation: BAA05834.1. AY429109 mRNA. Translation: AAR07904.1. DQ400918 Genomic DNA. Translation: ABD48960.1. BC101534 mRNA. Translation: AAI01535.1. BC113523 mRNA. Translation: AAI13524.1.
IPI	IPI00018648.
PIR	A53572.
RefSeq	NP_000949.1. NM_000958.2.
UniGene	Hs.199248.
3D structure databases
ProteinModelPortal	P35408.
SMR	P35408. Positions 104-135, 313-346.
ModBase	Search...
Protein-protein interaction databases
STRING	P35408.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35408.
Polymorphism databases
DMDM	548476.
Proteomic databases
PRIDE	P35408.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000302472; ENSP00000302846; ENSG00000171522.
GeneID	5734.
KEGG	hsa:5734.
UCSC	uc003jlz.1. human.
Organism-specific databases
CTD	5734.
GeneCards	GC05P040679.
H-InvDB	HIX0032033.
HGNC	HGNC:9596. PTGER4.
HPA	HPA011226. HPA012756.
MIM	601586. gene.
neXtProt	NX_P35408.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074340.
HOGENOM	HBG713313.
HOVERGEN	HBG053556.
InParanoid	P35408.
OMA	FCRIGGS.
OrthoDB	EOG495ZS1.
PhylomeDB	P35408.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P35408.
Bgee	P35408.
CleanEx	HS_PTGER2. HS_PTGER4.
Genevestigator	P35408.
GermOnline	ENSG00000171522. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001758. Prost_EP4_rcpt. IPR008365. Prostanoid_rcpt. IPR001244. Prostglndn_DP_rcpt. [Graphical view]
KO	K04261.
PANTHER	PTHR11866:SF6. Prost_EP4_rcpt. 1 hit. PTHR11866. Prostanoid_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00428. PROSTAGLNDNR. PR01788. PROSTANOIDR. PR00586. PRSTNOIDEP4R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	22322.
SOURCE	Search...

Entry information

Entry name

PE2R4_HUMAN

Accession

Primary (citable) accession number: P35408
Secondary accession number(s): Q3MJ87

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.