P35407 (CXCR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-X-C chemokine receptor type 2
Short name=CXC-R2
Short name=CXCR-2

Alternative name(s):
GRO/MGSA receptor
High affinity interleukin-8 receptor B
Short name=IL-8R B
CD_antigen=CD182

Gene names

Name:	Cxcr2
Synonyms:	Il8rb

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated upon ligand binding; which is required for desensitization By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: Compara cellular defense response Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from direct assay PubMed 10975996. Source: RGD interleukin-8-mediated signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: InterPro neutrophil activation Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from mutant phenotype PubMed 10975996. Source: RGD phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara receptor internalization Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mast cell granule Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	C-X-C chemokine receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	C-X-C chemokine receptor type 2		PRO_0000069342
Regions
	Topological domain	1 – 47	47	Extracellular Potential
	Transmembrane	48 – 74	27	Helical; Name=1; Potential
	Topological domain	75 – 83	9	Cytoplasmic Potential
	Transmembrane	84 – 104	21	Helical; Name=2; Potential
	Topological domain	105 – 119	15	Extracellular Potential
	Transmembrane	120 – 141	22	Helical; Name=3; Potential
	Topological domain	142 – 162	21	Cytoplasmic Potential
	Transmembrane	163 – 182	20	Helical; Name=4; Potential
	Topological domain	183 – 207	25	Extracellular Potential
	Transmembrane	208 – 230	23	Helical; Name=5; Potential
	Topological domain	231 – 250	20	Cytoplasmic Potential
	Transmembrane	251 – 272	22	Helical; Name=6; Potential
	Topological domain	273 – 296	24	Extracellular Potential
	Transmembrane	297 – 314	18	Helical; Name=7; Potential
	Topological domain	315 – 359	45	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 195		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35407 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: EF547326A074ABDD	FASTA	359	40,532
	10 20 30 40 50 60 MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL 70 80 90 100 110 120 SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV 130 140 150 160 170 180 FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF 190 200 210 220 230 240 ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA 250 260 270 280 290 300 HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI 310 320 330 340 350 LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL

References

[1]	"Molecular cloning and characterization of a cDNA encoding the rat interleukin-8 receptor." Gobl A.E., Huang M.R., Wang S., Zhou Y., Oeberg K. Biochim. Biophys. Acta 1326:171-177(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	Konishi K., Shibata F., Watanabe K., Tsurufuji S., Nakagawa H., Fujioka M. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Liver.
[3]	"Identification of two rat genes orthologous to the human interleukin-8 receptors." Dunstan C.-A.N., Salafranca M.N., Adhikari S., Xia Y., Feng L., Harrison J.K. J. Biol. Chem. 271:32770-32776(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Lung and Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77797 mRNA. Translation: CAA54824.1. D63584 Genomic DNA. Translation: BAA09797.1. U70988 Genomic DNA. Translation: AAC52961.1.
IPI	IPI00198588.
PIR	S42096.
RefSeq	NP_058879.1. NM_017183.1.
UniGene	Rn.90347.
3D structure databases
ProteinModelPortal	P35407.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35407. 1 interaction.
MINT	MINT-271110.
STRING	10116.ENSRNOP00000019109.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35407.
Proteomic databases
PaxDb	P35407.
PRIDE	P35407.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000019109; ENSRNOP00000019109; ENSRNOG00000014269.
GeneID	29385.
KEGG	rno:29385.
UCSC	RGD:2906. rat.
Organism-specific databases
CTD	3579.
RGD	2906. Cxcr2.
Phylogenomic databases
eggNOG	NOG146068.
GeneTree	ENSGT00640000091250.
HOGENOM	HOG000234122.
HOVERGEN	HBG106917.
InParanoid	P35407.
KO	K05050.
OMA	CARRNDI.
OrthoDB	EOG4M0F2B.
Gene expression databases
Genevestigator	P35407.
GermOnline	ENSRNOG00000014269. Rattus norvegicus.
Family and domain databases
InterPro	IPR000174. Chemokine_CXCR. IPR000057. Chemokine_CXCR2. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00427. INTRLEUKIN8R. PR00573. INTRLEUKN8BR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608983.

Entry information

Entry name

CXCR2_RAT

Accession

Primary (citable) accession number: P35407

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program