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P35407 (CXCR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-X-C chemokine receptor type 2

Short name=CXC-R2
Short name=CXCR-2
Alternative name(s):
GRO/MGSA receptor
High affinity interleukin-8 receptor B
Short name=IL-8R B
CD_antigen=CD182
Gene names
Name:Cxcr2
Synonyms:Il8rb
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylated upon ligand binding; which is required for desensitization By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacute inflammatory response to antigenic stimulus

Inferred from mutant phenotype PubMed 15516486. Source: RGD

cellular defense response

Inferred from electronic annotation. Source: Ensembl

metanephric tubule morphogenesis

Inferred from mutant phenotype PubMed 17634442. Source: RGD

midbrain development

Inferred from expression pattern PubMed 18436867. Source: RGD

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 17634442. Source: RGD

negative regulation of neutrophil apoptotic process

Inferred from mutant phenotype PubMed 22312020. Source: RGD

neutrophil activation

Inferred from electronic annotation. Source: Ensembl

neutrophil chemotaxis

Inferred from mutant phenotype PubMed 10975996. Source: RGD

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from mutant phenotype PubMed 18832730. Source: RGD

positive regulation of cardiac muscle cell apoptotic process

Inferred from mutant phenotype PubMed 22562555. Source: RGD

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay PubMed 10975996. Source: RGD

positive regulation of leukocyte chemotaxis

Inferred from mutant phenotype PubMed 17717298. Source: RGD

positive regulation of neutrophil chemotaxis

Inferred from mutant phenotype PubMed 23947621. Source: RGD

positive regulation of vascular permeability

Inferred from mutant phenotype PubMed 19255141. Source: RGD

receptor internalization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mast cell granule

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninterleukin-8 receptor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359C-X-C chemokine receptor type 2
PRO_0000069342

Regions

Topological domain1 – 4747Extracellular Potential
Transmembrane48 – 7427Helical; Name=1; Potential
Topological domain75 – 839Cytoplasmic Potential
Transmembrane84 – 10421Helical; Name=2; Potential
Topological domain105 – 11915Extracellular Potential
Transmembrane120 – 14122Helical; Name=3; Potential
Topological domain142 – 16221Cytoplasmic Potential
Transmembrane163 – 18220Helical; Name=4; Potential
Topological domain183 – 20725Extracellular Potential
Transmembrane208 – 23023Helical; Name=5; Potential
Topological domain231 – 25020Cytoplasmic Potential
Transmembrane251 – 27222Helical; Name=6; Potential
Topological domain273 – 29624Extracellular Potential
Transmembrane297 – 31418Helical; Name=7; Potential
Topological domain315 – 35945Cytoplasmic Potential

Amino acid modifications

Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation2011N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Disulfide bond118 ↔ 195 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35407 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: EF547326A074ABDD

FASTA35940,532
        10         20         30         40         50         60 
MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL 

        70         80         90        100        110        120 
SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV 

       130        140        150        160        170        180 
FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF 

       190        200        210        220        230        240 
ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA 

       250        260        270        280        290        300 
HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI 

       310        320        330        340        350 
LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL 

« Hide

References

[1]"Molecular cloning and characterization of a cDNA encoding the rat interleukin-8 receptor."
Gobl A.E., Huang M.R., Wang S., Zhou Y., Oeberg K.
Biochim. Biophys. Acta 1326:171-177(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Lung.
[2]Konishi K., Shibata F., Watanabe K., Tsurufuji S., Nakagawa H., Fujioka M.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Wistar.
Tissue: Liver.
[3]"Identification of two rat genes orthologous to the human interleukin-8 receptors."
Dunstan C.-A.N., Salafranca M.N., Adhikari S., Xia Y., Feng L., Harrison J.K.
J. Biol. Chem. 271:32770-32776(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Wistar.
Tissue: Lung and Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77797 mRNA. Translation: CAA54824.1.
D63584 Genomic DNA. Translation: BAA09797.1.
U70988 Genomic DNA. Translation: AAC52961.1.
PIRS42096.
RefSeqNP_058879.1. NM_017183.1.
UniGeneRn.90347.

3D structure databases

ProteinModelPortalP35407.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP35407. 1 interaction.
MINTMINT-271110.
STRING10116.ENSRNOP00000019109.

Chemistry

GuidetoPHARMACOLOGY69.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP35407.

Proteomic databases

PaxDbP35407.
PRIDEP35407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019109; ENSRNOP00000019109; ENSRNOG00000014269.
GeneID29385.
KEGGrno:29385.
UCSCRGD:2906. rat.

Organism-specific databases

CTD3579.
RGD2906. Cxcr2.

Phylogenomic databases

eggNOGNOG146068.
GeneTreeENSGT00730000110292.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidP35407.
KOK05050.
OMAVITEDCK.
OrthoDBEOG7ZPNK9.
PhylomeDBP35407.
TreeFamTF330966.

Gene expression databases

GenevestigatorP35407.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000057. Chemokine_CXCR2.
IPR000174. Chemokine_CXCR_1/2.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00427. INTRLEUKIN8R.
PR00573. INTRLEUKN8BR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608983.
PROP35407.

Entry information

Entry nameCXCR2_RAT
AccessionPrimary (citable) accession number: P35407
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries