Reviewed, UniProtKB/Swiss-Prot P35407 (CXCR2_RAT)

Names and origin

Protein names

Recommended name:
    High affinity interleukin-8 receptor B
      Short name=IL-8R B
Alternative name(s):
    CXCR-2
    GRO/MGSA receptor
    CD_antigen=CD182

Gene names

Name:	Il8rb
Synonyms:	Cxcr2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated upon ligand binding; which is required for desensitization By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	interleukin-8 receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Mpp4	Q9QYH1	1	EBI-937591,EBI-937554

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	High affinity interleukin-8 receptor B		PRO_0000069342
Regions
	Topological domain	1 – 47	47	Extracellular Potential
	Transmembrane	48 – 74	27	1 Potential
	Topological domain	75 – 83	9	Cytoplasmic Potential
	Transmembrane	84 – 104	21	2 Potential
	Topological domain	105 – 119	15	Extracellular Potential
	Transmembrane	120 – 141	22	3 Potential
	Topological domain	142 – 162	21	Cytoplasmic Potential
	Transmembrane	163 – 182	20	4 Potential
	Topological domain	183 – 207	25	Extracellular Potential
	Transmembrane	208 – 230	23	5 Potential
	Topological domain	231 – 250	20	Cytoplasmic Potential
	Transmembrane	251 – 272	22	6 Potential
	Topological domain	273 – 296	24	Extracellular Potential
	Transmembrane	297 – 314	18	7 Potential
	Topological domain	315 – 359	45	Cytoplasmic Potential
Amino acid modifications
	Modified residue	346	1	Phosphoserine By similarity
	Modified residue	350	1	Phosphoserine By similarity
	Modified residue	351	1	Phosphoserine By similarity
	Modified residue	352	1	Phosphoserine By similarity
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 195		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35407-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: EF547326A074ABDD	FASTA	359	40,532
	10 20 30 40 50 60 MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL 70 80 90 100 110 120 SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV 130 140 150 160 170 180 FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF 190 200 210 220 230 240 ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA 250 260 270 280 290 300 HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI 310 320 330 340 350 LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL

References

[1]	"Molecular cloning and characterization of a cDNA encoding the rat interleukin-8 receptor." Gobl A.E., Huang M.R., Wang S., Zhou Y., Oeberg K. Biochim. Biophys. Acta 1326:171-177(1997) [PubMed: 9218548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	Konishi K., Shibata F., Watanabe K., Tsurufuji S., Nakagawa H., Fujioka M. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Liver.
[3]	"Identification of two rat genes orthologous to the human interleukin-8 receptors." Dunstan C.-A.N., Salafranca M.N., Adhikari S., Xia Y., Feng L., Harrison J.K. J. Biol. Chem. 271:32770-32776(1996) [PubMed: 8955112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Lung and Spleen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77797 mRNA. Translation: CAA54824.1. D63584 Genomic DNA. Translation: BAA09797.1. U70988 Genomic DNA. Translation: AAC52961.1.
IPI	IPI00198588.
PIR	S42096.
RefSeq	NP_058879.1.
UniGene	Rn.90347
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P35407. 1 interaction.
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSRNOG00000014269. Rattus norvegicus. [Contig view]
GeneID	29385.
KEGG	rno:29385.
Organism-specific databases
RGD	2906. Il8rb.
Phylogenomic databases
HOVERGEN	P35407.
OMA	P35407. IVHATST.
Gene expression databases
ArrayExpress	P35407.
GermOnline	ENSRNOG00000014269. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000174. IL8_rcpt. IPR000057. IL8B_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00427. INTRLEUKIN8R. PR00573. INTRLEUKN8BR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608983.

Entry information

Entry name

CXCR2_RAT

Accession

Primary (citable) accession number: P35407

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)