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P35401

- CRAC_DICDI

UniProt

P35401 - CRAC_DICDI

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Protein

Protein CRAC

Gene

dagA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Couples activated G protein to adenylyl cyclase signal transduction from surface cAMP receptor. Pianissimo a cytosolic regulator and CRAC, are both essential for activation of the enzyme adenylyl cyclase. Pianissimo and CRAC do not function redundantly. Both proteins are integral components of the adenylyl cyclase activation pathway.1 Publication

GO - Molecular functioni

  1. enzyme activator activity Source: dictyBase
  2. phosphatidylinositol-3,4,5-trisphosphate binding Source: dictyBase
  3. phosphatidylinositol-3,4-bisphosphate binding Source: dictyBase

GO - Biological processi

  1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: dictyBase
  2. aggregation involved in sorocarp development Source: dictyBase
  3. chemotaxis Source: dictyBase
  4. phosphatidylinositol-mediated signaling Source: dictyBase
  5. positive regulation of adenylate cyclase activity Source: dictyBase
  6. regulation of actin polymerization or depolymerization Source: dictyBase
  7. sorocarp development Source: dictyBase
  8. sporulation resulting in formation of a cellular spore Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CRAC
Gene namesi
Name:dagA
ORF Names:DDB_G0285161
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285161. dagA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. leading edge membrane Source: dictyBase
  3. plasma membrane Source: dictyBase
  4. pseudopodium Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

dagA and piaA double mutants require both proteins for reconstitution and activation of adenylyl cyclase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 698697Protein CRACPRO_0000079325Add
BLAST

Proteomic databases

PRIDEiP35401.

Expressioni

Developmental stagei

Tightly developmentally regulated.

Structurei

3D structure databases

ProteinModelPortaliP35401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 122101PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi350 – 36314Poly-ThrAdd
BLAST
Compositional biasi468 – 48013Poly-GlyAdd
BLAST
Compositional biasi613 – 62412Poly-SerAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP35401.
OMAiLAMIRCE.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35401-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKTERKKEL LELFEYEKIK GDVSYSSIMK KAGGNGKGFL DRYFALHRNY
60 70 80 90 100
ILYYKLGKSS LKPDDKQEPQ GYINLMDCNP DDTKEIAPLM FQISHKHRTY
110 120 130 140 150
IVKAKDESSM KQFLTLLIAR IRSLEKIDID KLGCTVVVLT KVKKFREVLT
160 170 180 190 200
NPLILPDRVS PEMAEEWVKQ MKNYNASFNL ADPFIKQVEQ ISEFCRGEVK
210 220 230 240 250
EYIDWFGGPE GPRLAMIRCE ETVLSNWVEY INKTSSEITT YQDNRFFRED
260 270 280 290 300
FKDIAVHLKN MTTFIDCYND YMIHCRKYNN NKPNTKFLEE KQTFKEYIEK
310 320 330 340 350
FIPKVASCND VSLNQFYDRS LIQSSDGIVT INTSGIKKTL INQSNIISIT
360 370 380 390 400
STTTTTTTTT TTTCSMPNMS NLIHSLDHTN LNIIDLNHSK SQQQLHPPPS
410 420 430 440 450
PHHQHLHHQI VSNSKDFNIS VSSNNFNDGN SEFPNLDINC DFDLTSASNL
460 470 480 490 500
SSPILSSEVP SNVVDPIGSG QGGGGSGGGG VTAVTEEAIN EKWHFDCNTS
510 520 530 540 550
MIFKPPSEDG RNEGSNMSTS SITSKMSLSL NGGFDMKWVY QCGYFKSKNM
560 570 580 590 600
GSISWNGKHW CWSHPRTSYK IKYIWDPTKQ SFLNIPFKSR VGATGGGSVP
610 620 630 640 650
SSQSTNNLQS STSSMSSLSS SSTSTTKRSH PTTLYPDYQF KDNLLTPIII
660 670 680 690
EGRHQPSLTL IDSPLTIPNA CLLTIAMTQY IQDALIHLSL GPKVLSSK
Length:698
Mass (Da):78,630
Last modified:December 4, 2007 - v4
Checksum:i3CC3D8FF6D9D849B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti653 – 6531R → G in AAA61782. (PubMed:8089184)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U06228 Genomic DNA. Translation: AAA61782.1.
AAFI02000075 Genomic DNA. Translation: EAL64837.1.
PIRiA54796.
RefSeqiXP_638365.1. XM_633273.1.

Genome annotation databases

EnsemblProtistsiDDB0191434; DDB0191434; DDB_G0285161.
GeneIDi8624989.
KEGGiddi:DDB_G0285161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U06228 Genomic DNA. Translation: AAA61782.1 .
AAFI02000075 Genomic DNA. Translation: EAL64837.1 .
PIRi A54796.
RefSeqi XP_638365.1. XM_633273.1.

3D structure databases

ProteinModelPortali P35401.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P35401.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191434 ; DDB0191434 ; DDB_G0285161 .
GeneIDi 8624989.
KEGGi ddi:DDB_G0285161.

Organism-specific databases

dictyBasei DDB_G0285161. dagA.

Phylogenomic databases

InParanoidi P35401.
OMAi LAMIRCE.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
[Graphical view ]
PROSITEi PS50003. PH_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium."
    Insall R., Kupsa A., Lilly P.J., Shaulsky G., Levin L.R., Loomis W.F., Devreotes P.N.
    J. Cell Biol. 126:1537-1545(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Identification of CRAC, a cytosolic regulator required for guanine nucleotide stimulation of adenylyl cyclase in Dictyostelium."
    Lilly P.J., Devreotes P.N.
    J. Biol. Chem. 269:14123-14129(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-9.
  4. "A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium."
    Chen M.-Y., Long Y., Devreotes P.N.
    Genes Dev. 11:3218-3231(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCRAC_DICDI
AccessioniPrimary (citable) accession number: P35401
Secondary accession number(s): Q54NJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 75 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3