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P35401 (CRAC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein CRAC
Gene names
Name:dagA
ORF Names:DDB_G0285161
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length698 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Couples activated G protein to adenylyl cyclase signal transduction from surface cAMP receptor. Pianissimo a cytosolic regulator and CRAC, are both essential for activation of the enzyme adenylyl cyclase. Pianissimo and CRAC do not function redundantly. Both proteins are integral components of the adenylyl cyclase activation pathway. Ref.4

Subcellular location

Cytoplasm.

Developmental stage

Tightly developmentally regulated.

Disruption phenotype

dagA and piaA double mutants require both proteins for reconstitution and activation of adenylyl cyclase. Ref.4

Sequence similarities

Contains 1 PH domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 15563608PubMed 17606871. Source: dictyBase

aggregation involved in sorocarp development

Inferred from mutant phenotype PubMed 15668169Ref.1. Source: dictyBase

chemotaxis

Inferred from mutant phenotype PubMed 15668169PubMed 9778249. Source: dictyBase

phosphatidylinositol-mediated signaling

Traceable author statement PubMed 15366706. Source: dictyBase

positive regulation of adenylate cyclase activity

Inferred from mutant phenotype PubMed 15668169Ref.1. Source: dictyBase

regulation of actin polymerization or depolymerization

Inferred from mutant phenotype PubMed 15668169. Source: dictyBase

sorocarp development

Inferred from mutant phenotype PubMed 17659086. Source: dictyBase

sporulation resulting in formation of a cellular spore

Traceable author statement PubMed 15473840. Source: dictyBase

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

leading edge membrane

Inferred from direct assay PubMed 15563608PubMed 9778249. Source: dictyBase

plasma membrane

Inferred from direct assay PubMed 23132928. Source: dictyBase

pseudopodium

Inferred from direct assay PubMed 23132928. Source: dictyBase

   Molecular_functionenzyme activator activity

Inferred from mutant phenotype Ref.1. Source: dictyBase

phosphatidylinositol-3,4,5-trisphosphate binding

Inferred from direct assay PubMed 15668169. Source: dictyBase

phosphatidylinositol-3,4-bisphosphate binding

Inferred from mutant phenotype PubMed 15668169. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 698697Protein CRAC
PRO_0000079325

Regions

Domain22 – 122101PH
Compositional bias350 – 36314Poly-Thr
Compositional bias468 – 48013Poly-Gly
Compositional bias613 – 62412Poly-Ser

Experimental info

Sequence conflict6531R → G in AAA61782. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P35401 [UniParc].

Last modified December 4, 2007. Version 4.
Checksum: 3CC3D8FF6D9D849B

FASTA69878,630
        10         20         30         40         50         60 
MGKTERKKEL LELFEYEKIK GDVSYSSIMK KAGGNGKGFL DRYFALHRNY ILYYKLGKSS 

        70         80         90        100        110        120 
LKPDDKQEPQ GYINLMDCNP DDTKEIAPLM FQISHKHRTY IVKAKDESSM KQFLTLLIAR 

       130        140        150        160        170        180 
IRSLEKIDID KLGCTVVVLT KVKKFREVLT NPLILPDRVS PEMAEEWVKQ MKNYNASFNL 

       190        200        210        220        230        240 
ADPFIKQVEQ ISEFCRGEVK EYIDWFGGPE GPRLAMIRCE ETVLSNWVEY INKTSSEITT 

       250        260        270        280        290        300 
YQDNRFFRED FKDIAVHLKN MTTFIDCYND YMIHCRKYNN NKPNTKFLEE KQTFKEYIEK 

       310        320        330        340        350        360 
FIPKVASCND VSLNQFYDRS LIQSSDGIVT INTSGIKKTL INQSNIISIT STTTTTTTTT 

       370        380        390        400        410        420 
TTTCSMPNMS NLIHSLDHTN LNIIDLNHSK SQQQLHPPPS PHHQHLHHQI VSNSKDFNIS 

       430        440        450        460        470        480 
VSSNNFNDGN SEFPNLDINC DFDLTSASNL SSPILSSEVP SNVVDPIGSG QGGGGSGGGG 

       490        500        510        520        530        540 
VTAVTEEAIN EKWHFDCNTS MIFKPPSEDG RNEGSNMSTS SITSKMSLSL NGGFDMKWVY 

       550        560        570        580        590        600 
QCGYFKSKNM GSISWNGKHW CWSHPRTSYK IKYIWDPTKQ SFLNIPFKSR VGATGGGSVP 

       610        620        630        640        650        660 
SSQSTNNLQS STSSMSSLSS SSTSTTKRSH PTTLYPDYQF KDNLLTPIII EGRHQPSLTL 

       670        680        690 
IDSPLTIPNA CLLTIAMTQY IQDALIHLSL GPKVLSSK 

« Hide

References

« Hide 'large scale' references
[1]"CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium."
Insall R., Kupsa A., Lilly P.J., Shaulsky G., Levin L.R., Loomis W.F., Devreotes P.N.
J. Cell Biol. 126:1537-1545(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Identification of CRAC, a cytosolic regulator required for guanine nucleotide stimulation of adenylyl cyclase in Dictyostelium."
Lilly P.J., Devreotes P.N.
J. Biol. Chem. 269:14123-14129(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-9.
[4]"A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium."
Chen M.-Y., Long Y., Devreotes P.N.
Genes Dev. 11:3218-3231(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U06228 Genomic DNA. Translation: AAA61782.1.
AAFI02000075 Genomic DNA. Translation: EAL64837.1.
PIRA54796.
RefSeqXP_638365.1. XM_633273.1.

3D structure databases

ProteinModelPortalP35401.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP35401.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191434; DDB0191434; DDB_G0285161.
GeneID8624989.
KEGGddi:DDB_G0285161.

Organism-specific databases

dictyBaseDDB_G0285161. dagA.

Phylogenomic databases

OMALAMIRCE.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRAC_DICDI
AccessionPrimary (citable) accession number: P35401
Secondary accession number(s): Q54NJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 72 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase