P35401 (CRAC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein CRAC

Gene names

Name:	dagA
ORF Names:	DDB_G0285161

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Couples activated G protein to adenylyl cyclase signal transduction from surface cAMP receptor. Pianissimo a cytosolic regulator and CRAC, are both essential for activation of the enzyme adenylyl cyclase. Pianissimo and CRAC do not function redundantly. Both proteins are integral components of the adenylyl cyclase activation pathway. Ref.4
Subcellular location	Cytoplasm.
Developmental stage	Tightly developmentally regulated.
Disruption phenotype	dagA and piaA double mutants require both proteins for reconstitution and activation of adenylyl cyclase. Ref.4
Sequence similarities	Contains 1 PH domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Developmental protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 15563608PubMed 17606871. Source: dictyBase aggregation involved in sorocarp development Inferred from mutant phenotype PubMed 15668169Ref.1. Source: dictyBase chemotaxis Inferred from mutant phenotype PubMed 15668169PubMed 9778249. Source: dictyBase phosphatidylinositol-mediated signaling Traceable author statement PubMed 15366706. Source: dictyBase positive regulation of adenylate cyclase activity Inferred from mutant phenotype PubMed 15668169Ref.1. Source: dictyBase regulation of actin polymerization or depolymerization Inferred from mutant phenotype PubMed 15668169. Source: dictyBase sporulation resulting in formation of a cellular spore Traceable author statement PubMed 15473840. Source: dictyBase
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell leading edge membrane Inferred from direct assay PubMed 15563608PubMed 9778249. Source: dictyBase
Molecular_function	enzyme activator activity Inferred from mutant phenotype Ref.1. Source: dictyBase phosphatidylinositol-3,4,5-trisphosphate binding Inferred from direct assay PubMed 15668169. Source: dictyBase phosphatidylinositol-3,4-bisphosphate binding Inferred from mutant phenotype PubMed 15668169. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 698	697	Protein CRAC		PRO_0000079325
Regions
	Domain	22 – 122	101	PH
	Compositional bias	350 – 363	14	Poly-Thr
	Compositional bias	468 – 480	13	Poly-Gly
	Compositional bias	613 – 624	12	Poly-Ser
Experimental info
	Sequence conflict	653	1	R → G in AAA61782. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35401 [UniParc]. Last modified December 4, 2007. Version 4. Checksum: 3CC3D8FF6D9D849B	FASTA	698	78,630
	10 20 30 40 50 60 MGKTERKKEL LELFEYEKIK GDVSYSSIMK KAGGNGKGFL DRYFALHRNY ILYYKLGKSS 70 80 90 100 110 120 LKPDDKQEPQ GYINLMDCNP DDTKEIAPLM FQISHKHRTY IVKAKDESSM KQFLTLLIAR 130 140 150 160 170 180 IRSLEKIDID KLGCTVVVLT KVKKFREVLT NPLILPDRVS PEMAEEWVKQ MKNYNASFNL 190 200 210 220 230 240 ADPFIKQVEQ ISEFCRGEVK EYIDWFGGPE GPRLAMIRCE ETVLSNWVEY INKTSSEITT 250 260 270 280 290 300 YQDNRFFRED FKDIAVHLKN MTTFIDCYND YMIHCRKYNN NKPNTKFLEE KQTFKEYIEK 310 320 330 340 350 360 FIPKVASCND VSLNQFYDRS LIQSSDGIVT INTSGIKKTL INQSNIISIT STTTTTTTTT 370 380 390 400 410 420 TTTCSMPNMS NLIHSLDHTN LNIIDLNHSK SQQQLHPPPS PHHQHLHHQI VSNSKDFNIS 430 440 450 460 470 480 VSSNNFNDGN SEFPNLDINC DFDLTSASNL SSPILSSEVP SNVVDPIGSG QGGGGSGGGG 490 500 510 520 530 540 VTAVTEEAIN EKWHFDCNTS MIFKPPSEDG RNEGSNMSTS SITSKMSLSL NGGFDMKWVY 550 560 570 580 590 600 QCGYFKSKNM GSISWNGKHW CWSHPRTSYK IKYIWDPTKQ SFLNIPFKSR VGATGGGSVP 610 620 630 640 650 660 SSQSTNNLQS STSSMSSLSS SSTSTTKRSH PTTLYPDYQF KDNLLTPIII EGRHQPSLTL 670 680 690 IDSPLTIPNA CLLTIAMTQY IQDALIHLSL GPKVLSSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium." Insall R., Kupsa A., Lilly P.J., Shaulsky G., Levin L.R., Loomis W.F., Devreotes P.N. J. Cell Biol. 126:1537-1545(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Identification of CRAC, a cytosolic regulator required for guanine nucleotide stimulation of adenylyl cyclase in Dictyostelium." Lilly P.J., Devreotes P.N. J. Biol. Chem. 269:14123-14129(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9.
[4]	"A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium." Chen M.-Y., Long Y., Devreotes P.N. Genes Dev. 11:3218-3231(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06228 Genomic DNA. Translation: AAA61782.1. AAFI02000075 Genomic DNA. Translation: EAL64837.1.
PIR	A54796.
RefSeq	XP_638365.1. XM_633273.1.
3D structure databases
ProteinModelPortal	P35401.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191434; DDB0191434; DDB_G0285161.
GeneID	8624989.
KEGG	ddi:DDB_G0285161.
Organism-specific databases
dictyBase	DDB_G0285161. dagA.
Phylogenomic databases
OMA	LAMIRCE.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRAC_DICDI

Accession

Primary (citable) accession number: P35401
Secondary accession number(s): Q54NJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 67 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)