P35400 (GRM7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metabotropic glutamate receptor 7
Short name=mGluR7

Gene names

Name:	Grm7
Synonyms:	Gprc1g, Mglur7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	915 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
Subunit structure	Interacts with PICK1. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely distributed throughout the brain.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	learning or memory Traceable author statement PubMed 12021391. Source: UniProtKB negative regulation of glutamate secretion Inferred from direct assay PubMed 11825890PubMed 12692128. Source: RGD sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 9300765. Source: RGD asymmetric synapse Inferred from direct assay PubMed 9300765. Source: RGD cell surface Inferred from direct assay PubMed 16890965. Source: UniProtKB dendritic shaft Inferred from direct assay PubMed 12823458. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 8929965PubMed 9300765. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from direct assay PubMed 8764662. Source: UniProtKB presynaptic active zone Inferred from direct assay PubMed 8764662. Source: UniProtKB presynaptic membrane Inferred from direct assay PubMed 9300765. Source: RGD protein complex Inferred from direct assay PubMed 11007882. Source: RGD rough endoplasmic reticulum Inferred from direct assay PubMed 9300765. Source: RGD terminal bouton Inferred from direct assay PubMed 12823458. Source: RGD
Molecular_function	glutamate binding Inferred from direct assay PubMed 11825890PubMed 12692128PubMed 17620729. Source: RGD group III metabotropic glutamate receptor activity Inferred from direct assay PubMed 12692128. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 17620729. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 34

34

Potential

Chain

35 – 915

881

Metabotropic glutamate receptor 7

PRO_0000012940

Regions

Topological domain

35 – 590

556

Extracellular Potential

Transmembrane

591 – 615

25

Helical; Name=1; Potential

Topological domain

616 – 627

12

Cytoplasmic Potential

Transmembrane

628 – 648

21

Helical; Name=2; Potential

Topological domain

649 – 654

6

Extracellular Potential

Transmembrane

655 – 675

21

Helical; Name=3; Potential

Topological domain

676 – 702

27

Cytoplasmic Potential

Transmembrane

703 – 723

21

Helical; Name=4; Potential

Topological domain

724 – 753

30

Extracellular Potential

Transmembrane

754 – 775

22

Helical; Name=5; Potential

Topological domain

776 – 788

13

Cytoplasmic Potential

Transmembrane

789 – 810

22

Helical; Name=6; Potential

Topological domain

811 – 825

15

Extracellular Potential

Transmembrane

826 – 850

25

Helical; Name=7; Potential

Topological domain

851 – 915

65

Cytoplasmic Potential

Amino acid modifications

Glycosylation

98

1

N-linked (GlcNAc...) Potential

Glycosylation

458

1

N-linked (GlcNAc...) Potential

Glycosylation

486

1

N-linked (GlcNAc...) Potential

Glycosylation

572

1

N-linked (GlcNAc...) Potential

Disulfide bond

67 ↔ 109

Ref.4

Disulfide bond

249 ↔ 541

By similarity

Disulfide bond

374 ↔ 390

Ref.4

Disulfide bond

430 ↔ 437

Ref.4

Disulfide bond

523 ↔ 542

By similarity

Disulfide bond

527 ↔ 545

By similarity

Disulfide bond

548 ↔ 560

By similarity

Disulfide bond

563 ↔ 576

By similarity

Secondary structure

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915

Helix Strand Turn

Details...

Beta strand

42 – 45

4

Beta strand

48 – 55

8

Beta strand

58 – 60

3

Beta strand

62 – 64

3

Beta strand

66 – 70

5

Helix

72 – 91

20

Beta strand

93 – 98

6

Beta strand

101 – 107

7

Helix

112 – 118

7

Helix

119 – 126

8

Beta strand

150 – 154

5

Helix

159 – 165

7

Helix

168 – 171

4

Beta strand

176 – 180

5

Helix

184 – 187

4

Turn

189 – 192

4

Beta strand

193 – 199

7

Helix

202 – 215

14

Beta strand

221 – 228

8

Helix

229 – 244

16

Beta strand

250 – 256

7

Helix

265 – 274

10

Beta strand

282 – 286

5

Helix

289 – 301

13

Turn

305 – 307

3

Beta strand

308 – 312

5

Turn

314 – 318

5

Helix

321 – 323

3

Helix

327 – 330

4

Beta strand

334 – 339

6

Helix

344 – 350

7

Turn

355 – 357

3

Helix

364 – 371

8

Helix

394 – 397

4

Helix

408 – 429

22

Turn

438 – 443

6

Helix

445 – 452

8

Beta strand

477 – 483

7

Beta strand

491 – 504

14

Beta strand

506 – 508

3

Sequences

	Sequence		Length	Mass (Da)
	P35400 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: F28AFC4C6454A6C2	FASTA	915	102,232
	10 20 30 40 50 60 MVQLGKLLRV LTLMKFPCCV LEVLLCVLAA AARGQEMYAP HSIRIEGDVT LGGLFPVHAK 70 80 90 100 110 120 GPSGVPCGDI KRENGIHRLE AMLYALDQIN SDPNLLPNVT LGARILDTCS RDTYALEQSL 130 140 150 160 170 180 TFVQALIQKD TSDVRCTNGE PPVFVKPEKV VGVIGASGSS VSIMVANILR LFQIPQISYA 190 200 210 220 230 240 STAPELSDDR RYDFFSRVVP PDSFQAQAMV DIVKALGWNY VSTLASEGSY GEKGVESFTQ 250 260 270 280 290 300 ISKEAGGLCI AQSVRIPQER KDRTIDFDRI IKQLLDTPNS RAVVIFANDE DIKQILAAAK 310 320 330 340 350 360 RADQVGHFLW VGSDSWGSKI NPLHQHEDIA EGAITIQPKR ATVEGFDAYF TSRTLENNRR 370 380 390 400 410 420 NVWFAEYWEE NFNCKLTISG SKKEDTDRKC TGQERIGKDS NYEQEGKVQF VIDAVYAMAH 430 440 450 460 470 480 ALHHMNKDLC ADYRGVCPEM EQAGGKKLLK YIRHVNFNGS AGTPVMFNKN GDAPGRYDIF 490 500 510 520 530 540 QYQTTNTTNP GYRLIGQWTD ELQLNIEDMQ WGKGVREIPS SVCTLPCKPG QRKKTQKGTP 550 560 570 580 590 600 CCWTCEPCDG YQYQFDEMTC QHCPYDQRPN ENRTGCQNIP IIKLEWHSPW AVIPVFLAML 610 620 630 640 650 660 GIIATIFVMA TFIRYNDTPI VRASGRELSY VLLTGIFLCY IITFLMIAKP DVAVCSFRRV 670 680 690 700 710 720 FLGLGMCISY AALLTKTNRI YRIFEQGKKS VTAPRLISPT SQLAITSSLI SVQLLGVFIW 730 740 750 760 770 780 FGVDPPNIII DYDEHKTMNP EQARGVLKCD ITDLQIICSL GYSILLMVTC TVYAIKTRGV 790 800 810 820 830 840 PENFNEAKPI GFTMYTTCIV WLAFIPIFFG TAQSAEKLYI QTTTLTISMN LSASVALGML 850 860 870 880 890 900 YMPKVYIIIF HPELNVQKRK RSFKAVVTAA TMSSRLSHKP SDRPNGEAKT ELCENVDPNS 910 PAAKKKYVSY NNLVI

References

[1]	"Molecular characterization of a new metabotropic glutamate receptor mGluR7 coupled to inhibitory cyclic AMP signal transduction." Okamoto N., Hori S., Akazawa C., Hayashi Y., Shigemoto R., Mizuno N., Nakanishi S. J. Biol. Chem. 269:1231-1236(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Cloning and expression of a new member of the L-2-amino-4-phosphonobutyric acid-sensitive class of metabotropic glutamate receptors." Saugstad J.A., Kinzie J.M., Mulvihill E.R., Segerson T.P., Westbrook G.L. Mol. Pharmacol. 45:367-372(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Olfactory bulb.
[3]	"Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1." El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H. Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PICK1.
[4]	"Structures of the extracellular regions of the group II/III metabotropic glutamate receptors." Muto T., Tsuchiya D., Morikawa K., Jingami H. Proc. Natl. Acad. Sci. U.S.A. 104:3759-3764(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 33-521, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D16817 mRNA. Translation: BAA04092.1.
U06832 mRNA. Translation: AAA20655.1.

IPI

IPI00198587.

PIR

A49874.

RefSeq

NP_112302.1. NM_031040.1.

UniGene

Rn.10409.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E4Z	X-ray	3.30	A	33-521	[»]

ProteinModelPortal

P35400.

SMR

P35400. Positions 40-511.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-41145N.

MINT

MINT-146474.

STRING

10116.ENSRNOP00000053411.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P35400.

Proteomic databases

PRIDE

P35400.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

81672.

KEGG

rno:81672.

Organism-specific databases

CTD

2917.

RGD

619857. Grm7.

Phylogenomic databases

eggNOG

NOG295200.

HOVERGEN

HBG107965.

KO

K04608.

Gene expression databases

Genevestigator

P35400.

Family and domain databases

InterPro

IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001883. GPCR_3_mtglu_rcpt_7.
[Graphical view]

Pfam

PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]

PRINTS

PR00248. GPCRMGR.
PR01057. MTABOTROPC7R.
PR00593. MTABOTROPICR.

PROSITE

PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P35400.

ChEMBL

CHEMBL3879.

EvolutionaryTrace

P35400.

NextBio

615264.

Entry information

Entry name

GRM7_RAT

Accession

Primary (citable) accession number: P35400

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program