Reviewed, UniProtKB/Swiss-Prot P35396 (PPARD_MOUSE)

Names and origin

Protein names

Recommended name:
    Peroxisome proliferator-activated receptor delta
      Short name=PPAR-delta
Alternative name(s):
    PPAR-beta
    Nuclear receptor subfamily 1 group C member 2
    Nuclear hormone receptor 1
      Short name=NUC1

Gene names

Name:	Ppard
Synonyms:	Nr1c2, Pparb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids.
Subunit structure	Heterodimer with the retinoid X receptor.
Subcellular location	Nucleus.
Tissue specificity	Heart, adrenal and intestine.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Receptor
Gene Ontology (GO)
Biological process	anagen Inferred from mutant phenotype. Source: MGI axon ensheathment Inferred from mutant phenotype. Source: MGI cell differentiation Inferred from direct assay. Source: MGI cell-substrate adhesion Inferred from mutant phenotype. Source: MGI embryo implantation Traceable author statement. Source: UniProtKB fatty acid beta-oxidation Inferred from expression pattern. Source: UniProtKB fatty acid transport Inferred from expression pattern. Source: UniProtKB keratinocyte migration Inferred from mutant phenotype. Source: MGI keratinocyte proliferation Inferred from mutant phenotype. Source: MGI negative regulation of epithelial cell proliferation Inferred from genetic interaction. Source: MGI negative regulation of transcription from RNA polymerase II promoter Ref.2 Inferred from direct assay. Source: MGI placenta development Inferred from mutant phenotype. Source: MGI positive regulation of cell proliferation Inferred from mutant phenotype. Source: MGI positive regulation of phosphoinositide 3-kinase cascade Inferred from direct assay. Source: MGI regulation of fat cell differentiation Inferred from mutant phenotype. Source: MGI regulation of insulin secretion Non-traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW wound healing Inferred from mutant phenotype. Source: MGI
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	enzyme activator activity Traceable author statement. Source: UniProtKB prostacyclin receptor activity Traceable author statement. Source: UniProtKB protein heterodimerization activity Traceable author statement. Source: UniProtKB retinoid X receptor binding Traceable author statement. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro transcription repressor activity Ref.2 Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRKAA1	Q13131	1	EBI-1809541,EBI-1181405	From a different organism.
PRKAA2	P54646	1	EBI-1809541,EBI-1383852	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Peroxisome proliferator-activated receptor delta		PRO_0000053487
Regions
	DNA binding	70 – 144	75	Nuclear receptor
	Zinc finger	73 – 93	21	NR C4-type
	Zinc finger	110 – 132	23	NR C4-type
	Region	253 – 440	188	Ligand-binding Potential
Experimental info
	Sequence conflict	149 – 150	2	EA → DG in AAA19972. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35396-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 58E0F595DD193FDA	FASTA	440	49,715
	10 20 30 40 50 60 MEQPQEETPE AREEEKEEVA MGDGAPELNG GPEHTLPSSS CADLSQNSSP SSLLDQLQMG 70 80 90 100 110 120 CDGASGGSLN MECRVCGDKA SGFHYGVHAC EGCKGFFRRT IRMKLEYEKC DRICKIQKKN 130 140 150 160 170 180 RNKCQYCRFQ KCLALGMSHN AIRFGRMPEA EKRKLVAGLT ASEGCQHNPQ LADLKAFSKH 190 200 210 220 230 240 IYNAYLKNFN MTKKKARSIL TGKSSHNAPF VIHDIETLWQ AEKGLVWKQL VNGLPPYNEI 250 260 270 280 290 300 SVHVFYRCQS TTVETVRELT EFAKNIPNFS SLFLNDQVTL LKYGVHEAIF AMLASIVNKD 310 320 330 340 350 360 GLLVANGSGF VTHEFLRSLR KPFSDIIEPK FEFAVKFNAL ELDDSDLALF IAAIILCGDR 370 380 390 400 410 420 PGLMNVPQVE AIQDTILRAL EFHLQVNHPD SQYLFPKLLQ KMADLRQLVT EHAQMMQWLK 430 440 KTESETLLHP LLQEIYKDMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a protein that mediates transcriptional effects of fatty acids in preadipocytes. Homology to peroxisome proliferator-activated receptors." Amri E.-Z., Bonino F., Ailhaud G., Abumrad N.A., Grimaldi P.A. J. Biol. Chem. 270:2367-2371(1995) [PubMed: 7836471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Adipocyte.
[2]	"Differential expression and activation of a family of murine peroxisome proliferator-activated receptors." Kliewer S.A., Forman B.M., Blumberg B., Ong E.S., Borgmeyer U., Mangelsdorf D.J., Umesono K., Evans R.M. Proc. Natl. Acad. Sci. U.S.A. 91:7355-7359(1994) [PubMed: 8041794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Identification of two mPPAR related receptors and evidence for the existence of five subfamily members." Chen F., Law S.W., O'Malley B.W. Biochem. Biophys. Res. Commun. 196:671-677(1993) [PubMed: 8240342] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-145. Strain: BALB/c. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L28116 mRNA. Translation: AAA63394.1. U10375 mRNA. Translation: AAA19972.1. BC070398 mRNA. Translation: AAH70398.1. U01665 mRNA. Translation: AAA03332.1. Different initiation.
IPI	IPI00271799.
PIR	I55442.
RefSeq	NP_035275.1.
UniGene	Mm.328914
3D structure databases
HSSP	HSSP built from PDB template 3GWX based on UniProtKB Q03181.
SMR	P35396. Positions 170-440.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35396. 2 interactions.
PTM databases
PhosphoSite	P35396.
Genome annotation databases
Ensembl	ENSMUSG00000002250. Mus musculus. [Contig view]
GeneID	19015.
KEGG	mmu:19015.
Organism-specific databases
MGI	MGI:101884. Ppard.
Phylogenomic databases
HOVERGEN	P35396.
OMA	P35396. MEQPQEE.
Gene expression databases
ArrayExpress	P35396.
Bgee	P35396.
CleanEx	MM_PPARD.
GermOnline	ENSMUSG00000002250. Mus musculus.
Family and domain databases
InterPro	IPR003074. 1Cnucl_rcpt. IPR003075. 1Cnucl_rcpt_B. IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR001723. Str_hrmn_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:1.10.565.10. Nucl_hrmn_rcpt_lig_bd. 1 hit. G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR01288. PROXISOMEPAR. PR01290. PROXISOMPABR. PR00398. STRDHORMONER. PR00047. STROIDFINGER.
ProDom	PD000035. Znf_C4steroid. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295440.
SOURCE	Search...

Entry information

Entry name

PPARD_MOUSE

Accession

Primary (citable) accession number: P35396
Secondary accession number(s): P37239

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)