P35396 (PPARD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisome proliferator-activated receptor delta
Short name=PPAR-delta

Alternative name(s):
Nuclear hormone receptor 1
Short name=NUC1
Nuclear receptor subfamily 1 group C member 2
Peroxisome proliferator-activated receptor beta
Short name=PPAR-beta

Gene names

Name:	Ppard
Synonyms:	Nr1c2, Pparb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand By similarity.
Subunit structure	Heterodimer with the retinoid X receptor.
Subcellular location	Nucleus.
Tissue specificity	Heart, adrenal and intestine.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Sequence caution	The sequence AAA03332.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adipose tissue development Inferred from mutant phenotype PubMed 11756685. Source: MGI anagen Inferred from mutant phenotype PubMed 15713628. Source: MGI apoptotic process Inferred from electronic annotation. Source: Compara axon ensheathment Inferred from mutant phenotype PubMed 10866668. Source: MGI cell differentiation Inferred from direct assay PubMed 16581799. Source: MGI cell-substrate adhesion Inferred from mutant phenotype PubMed 11514592. Source: MGI embryo implantation Traceable author statement PubMed 10754535PubMed 15001550. Source: UniProtKB fatty acid beta-oxidation Inferred from expression pattern PubMed 14676330. Source: UniProtKB fatty acid transport Inferred from expression pattern PubMed 14676330. Source: UniProtKB keratinocyte migration Inferred from mutant phenotype PubMed 11514592. Source: MGI keratinocyte proliferation Inferred from mutant phenotype PubMed 11511099. Source: MGI negative regulation of epithelial cell proliferation Inferred from genetic interaction PubMed 14758356. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.2. Source: MGI placenta development Inferred from mutant phenotype PubMed 11756685. Source: MGI positive regulation of cell proliferation Inferred from mutant phenotype PubMed 11756685. Source: MGI positive regulation of phosphatidylinositol 3-kinase cascade Inferred from direct assay PubMed 16581799. Source: MGI positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB regulation of fat cell differentiation Inferred from mutant phenotype PubMed 11756685. Source: MGI regulation of insulin secretion Non-traceable author statement PubMed 14676330. Source: UniProtKB wound healing Inferred from mutant phenotype PubMed 11514592. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay Ref.2. Source: MGI drug binding Inferred from sequence or structural similarity. Source: UniProtKB enzyme activator activity Traceable author statement PubMed 14677574. Source: UniProtKB ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB linoleic acid binding Inferred from sequence or structural similarity. Source: UniProtKB prostacyclin receptor activity Traceable author statement PubMed 10754535. Source: UniProtKB protein heterodimerization activity Traceable author statement PubMed 15001550. Source: UniProtKB retinoid X receptor binding Traceable author statement PubMed 15001550. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Peroxisome proliferator-activated receptor delta		PRO_0000053487
Regions
	DNA binding	70 – 144	75	Nuclear receptor
	Zinc finger	73 – 93	21	NR C4-type
	Zinc finger	110 – 132	23	NR C4-type
	Region	253 – 440	188	Ligand-binding Potential
Experimental info
	Sequence conflict	149 – 150	2	EA → DG in AAA19972. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35396 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 58E0F595DD193FDA	FASTA	440	49,715
	10 20 30 40 50 60 MEQPQEETPE AREEEKEEVA MGDGAPELNG GPEHTLPSSS CADLSQNSSP SSLLDQLQMG 70 80 90 100 110 120 CDGASGGSLN MECRVCGDKA SGFHYGVHAC EGCKGFFRRT IRMKLEYEKC DRICKIQKKN 130 140 150 160 170 180 RNKCQYCRFQ KCLALGMSHN AIRFGRMPEA EKRKLVAGLT ASEGCQHNPQ LADLKAFSKH 190 200 210 220 230 240 IYNAYLKNFN MTKKKARSIL TGKSSHNAPF VIHDIETLWQ AEKGLVWKQL VNGLPPYNEI 250 260 270 280 290 300 SVHVFYRCQS TTVETVRELT EFAKNIPNFS SLFLNDQVTL LKYGVHEAIF AMLASIVNKD 310 320 330 340 350 360 GLLVANGSGF VTHEFLRSLR KPFSDIIEPK FEFAVKFNAL ELDDSDLALF IAAIILCGDR 370 380 390 400 410 420 PGLMNVPQVE AIQDTILRAL EFHLQVNHPD SQYLFPKLLQ KMADLRQLVT EHAQMMQWLK 430 440 KTESETLLHP LLQEIYKDMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a protein that mediates transcriptional effects of fatty acids in preadipocytes. Homology to peroxisome proliferator-activated receptors." Amri E.-Z., Bonino F., Ailhaud G., Abumrad N.A., Grimaldi P.A. J. Biol. Chem. 270:2367-2371(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Adipocyte.
[2]	"Differential expression and activation of a family of murine peroxisome proliferator-activated receptors." Kliewer S.A., Forman B.M., Blumberg B., Ong E.S., Borgmeyer U., Mangelsdorf D.J., Umesono K., Evans R.M. Proc. Natl. Acad. Sci. U.S.A. 91:7355-7359(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Identification of two mPPAR related receptors and evidence for the existence of five subfamily members." Chen F., Law S.W., O'Malley B.W. Biochem. Biophys. Res. Commun. 196:671-677(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-145. Strain: BALB/c. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L28116 mRNA. Translation: AAA63394.1. U10375 mRNA. Translation: AAA19972.1. BC070398 mRNA. Translation: AAH70398.1. U01665 mRNA. Translation: AAA03332.1. Different initiation.
IPI	IPI00271799.
PIR	I55442.
RefSeq	NP_035275.1. NM_011145.3.
UniGene	Mm.328914.
3D structure databases
ProteinModelPortal	P35396.
SMR	P35396. Positions 64-440.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29851N.
IntAct	P35396. 3 interactions.
PTM databases
PhosphoSite	P35396.
Proteomic databases
PRIDE	P35396.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000002320; ENSMUSP00000002320; ENSMUSG00000002250.
GeneID	19015.
KEGG	mmu:19015.
UCSC	uc008bqk.1. mouse.
Organism-specific databases
CTD	5467.
MGI	MGI:101884. Ppard.
Phylogenomic databases
eggNOG	NOG266867.
HOGENOM	HOG000261626.
HOVERGEN	HBG106004.
InParanoid	P35396.
KO	K04504.
OMA	SSLFLND.
OrthoDB	EOG4FFD1Q.
Gene expression databases
ArrayExpress	P35396.
Bgee	P35396.
CleanEx	MM_PPARD.
Genevestigator	P35396.
GermOnline	ENSMUSG00000002250. Mus musculus.
Family and domain databases
Gene3D	1.10.565.10. 2 hits. 3.30.50.10. 1 hit.
InterPro	IPR003074. 1Cnucl_rcpt. IPR003075. 1Cnucl_rcpt_B. IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR001723. Str_hrmn_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR01288. PROXISOMEPAR. PR01290. PROXISOMPABR. PR00398. STRDHORMONER. PR00047. STROIDFINGER.
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
SUPFAM	SSF48508. Str_ncl_receptor. 1 hit.
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P35396.
ChEMBL	CHEMBL2458.
NextBio	295440.
SOURCE	Search...

Entry information

Entry name

PPARD_MOUSE

Accession

Primary (citable) accession number: P35396
Secondary accession number(s): P37239

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program