P35392 (BLAC_STRFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:

blaF

Organism

Streptomyces fradiae (Streptomyces roseoflavus)

Taxonomic identifier

1906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 306	272	Beta-lactamase		PRO_0000017018
Regions
	Region	250 – 252	3	Substrate binding By similarity
Sites
	Active site	82	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35392 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 8FAEF14220812A17	FASTA	306	32,931
	10 20 30 40 50 60 MDRTTARPNR RAVLATGVGA ALAATAAAAG PAHAAPGRGA RVEGRLRALE RTHDARLGAF 70 80 90 100 110 120 AYDTGTGRTV AYRADERFPI ASMFKTIAVA AVLRDLDRDG EVLARRVHYT ADYVKRSGYS 130 140 150 160 170 180 PVTGLPENVA NGMTVAELCE ATLTRSDNTA ANLLLRDLGG PTAVTRFCRS VGDHVTRLDR 190 200 210 220 230 240 WEPELNSAEP GRVTDTTSPR AIGRTYGRLI LGDLLAAHDR ERLTRWMLDN RTSDERFRKG 250 260 270 280 290 300 LPADWLLADK TGGGDYGTNN DAGVAWPPGR PPVVLAVQTT RFTPDAEADN VLVAEAARLL AEAMTD

References

[1]	"Molecular analysis of beta-lactamases from four species of Streptomyces: comparison of amino acid sequences with those of other beta-lactamases." Forsman M., Haeggstroem B., Lindgren L., Jaurin B. J. Gen. Microbiol. 136:589-598(1990) [PubMed: 2391494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB 8233 / NRRL B-1195 / VKM Ac-150.
[2]	"Mutagenic analysis of the promoter of the Streptomyces fradiae beta-lactamase-encoding gene." Forsman M., Granstroem M. Gene 121:87-94(1992) [PubMed: 1385267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34179 Genomic DNA. Translation: AAA26709.1. M94255 Genomic DNA. Translation: AAC41397.1.
PIR	B45822.
3D structure databases
ProteinModelPortal	P35392.
SMR	P35392. Positions 43-304.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR006311. TAT_signal. [Graphical view]
Gene3D	G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_STRFR

Accession

Primary (citable) accession number: P35392
Secondary accession number(s): P72435

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 31, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program