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P35392 (BLAC_STRFR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Penicillinase
Gene names
Name:blaF
OrganismStreptomyces fradiae (Streptomyces roseoflavus)
Taxonomic identifier1906 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434Tat-type signal Potential
Chain35 – 306272Beta-lactamase
PRO_0000017018

Regions

Region250 – 2523Substrate binding By similarity

Sites

Active site821Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P35392 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 8FAEF14220812A17

FASTA30632,931
        10         20         30         40         50         60 
MDRTTARPNR RAVLATGVGA ALAATAAAAG PAHAAPGRGA RVEGRLRALE RTHDARLGAF 

        70         80         90        100        110        120 
AYDTGTGRTV AYRADERFPI ASMFKTIAVA AVLRDLDRDG EVLARRVHYT ADYVKRSGYS 

       130        140        150        160        170        180 
PVTGLPENVA NGMTVAELCE ATLTRSDNTA ANLLLRDLGG PTAVTRFCRS VGDHVTRLDR 

       190        200        210        220        230        240 
WEPELNSAEP GRVTDTTSPR AIGRTYGRLI LGDLLAAHDR ERLTRWMLDN RTSDERFRKG 

       250        260        270        280        290        300 
LPADWLLADK TGGGDYGTNN DAGVAWPPGR PPVVLAVQTT RFTPDAEADN VLVAEAARLL 


AEAMTD 

« Hide

References

[1]"Molecular analysis of beta-lactamases from four species of Streptomyces: comparison of amino acid sequences with those of other beta-lactamases."
Forsman M., Haeggstroem B., Lindgren L., Jaurin B.
J. Gen. Microbiol. 136:589-598(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB 8233 / NRRL B-1195 / VKM Ac-150.
[2]"Mutagenic analysis of the promoter of the Streptomyces fradiae beta-lactamase-encoding gene."
Forsman M., Granstroem M.
Gene 121:87-94(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34179 Genomic DNA. Translation: AAA26709.1.
M94255 Genomic DNA. Translation: AAC41397.1.
PIRB45822.

3D structure databases

ProteinModelPortalP35392.
SMRP35392. Positions 43-304.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR006311. TAT_signal.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLAC_STRFR
AccessionPrimary (citable) accession number: P35392
Secondary accession number(s): P72435
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families