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P35392

- BLAC_STRFR

UniProt

P35392 - BLAC_STRFR

Protein

Beta-lactamase

Gene

blaF

Organism
Streptomyces fradiae (Streptomyces roseoflavus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei82 – 821Acyl-ester intermediateBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:blaF
    OrganismiStreptomyces fradiae (Streptomyces roseoflavus)
    Taxonomic identifieri1906 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Tat-type signalPROSITE-ProRule annotationAdd
    BLAST
    Chaini35 – 306272Beta-lactamasePRO_0000017018Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Structurei

    3D structure databases

    ProteinModelPortaliP35392.
    SMRiP35392. Positions 43-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni250 – 2523Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35392-1 [UniParc]FASTAAdd to Basket

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    MDRTTARPNR RAVLATGVGA ALAATAAAAG PAHAAPGRGA RVEGRLRALE    50
    RTHDARLGAF AYDTGTGRTV AYRADERFPI ASMFKTIAVA AVLRDLDRDG 100
    EVLARRVHYT ADYVKRSGYS PVTGLPENVA NGMTVAELCE ATLTRSDNTA 150
    ANLLLRDLGG PTAVTRFCRS VGDHVTRLDR WEPELNSAEP GRVTDTTSPR 200
    AIGRTYGRLI LGDLLAAHDR ERLTRWMLDN RTSDERFRKG LPADWLLADK 250
    TGGGDYGTNN DAGVAWPPGR PPVVLAVQTT RFTPDAEADN VLVAEAARLL 300
    AEAMTD 306
    Length:306
    Mass (Da):32,931
    Last modified:June 1, 1994 - v1
    Checksum:i8FAEF14220812A17
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34179 Genomic DNA. Translation: AAA26709.1.
    M94255 Genomic DNA. Translation: AAC41397.1.
    PIRiB45822.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34179 Genomic DNA. Translation: AAA26709.1 .
    M94255 Genomic DNA. Translation: AAC41397.1 .
    PIRi B45822.

    3D structure databases

    ProteinModelPortali P35392.
    SMRi P35392. Positions 43-304.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of beta-lactamases from four species of Streptomyces: comparison of amino acid sequences with those of other beta-lactamases."
      Forsman M., Haeggstroem B., Lindgren L., Jaurin B.
      J. Gen. Microbiol. 136:589-598(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB 8233 / NRRL B-1195 / VKM Ac-150.
    2. "Mutagenic analysis of the promoter of the Streptomyces fradiae beta-lactamase-encoding gene."
      Forsman M., Granstroem M.
      Gene 121:87-94(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.

    Entry informationi

    Entry nameiBLAC_STRFR
    AccessioniPrimary (citable) accession number: P35392
    Secondary accession number(s): P72435
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3