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Protein

P2Y purinoceptor 2

Gene

P2ry2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
P2Y purinoceptor 2
Short name:
P2Y2
Alternative name(s):
ATP receptor
P2U purinoceptor 1
Short name:
P2U1
Purinergic receptor
Gene namesi
Name:P2ry2
Synonyms:P2ru1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105107. P2ry2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232ExtracellularSequence analysisAdd
BLAST
Transmembranei33 – 5927Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini60 – 7011CytoplasmicSequence analysisAdd
BLAST
Transmembranei71 – 9323Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini94 – 11017ExtracellularSequence analysisAdd
BLAST
Transmembranei111 – 12919Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini130 – 15223CytoplasmicSequence analysisAdd
BLAST
Transmembranei153 – 17220Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini173 – 19422ExtracellularSequence analysisAdd
BLAST
Transmembranei195 – 22026Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini221 – 24626CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26923Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini270 – 28718ExtracellularSequence analysisAdd
BLAST
Transmembranei288 – 30922Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini310 – 37364CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi107 – 1071K → I: No effect on receptor activation. 1 Publication
Mutagenesisi110 – 1101R → L: No effect on receptor activation. 1 Publication
Mutagenesisi262 – 2621H → L: Decrease in receptor activation. 1 Publication
Mutagenesisi265 – 2651R → L: Decrease in receptor activation. 1 Publication
Mutagenesisi289 – 2891K → I: No effect on receptor activation. 1 Publication
Mutagenesisi292 – 2921R → L: Decrease in receptor activation. 1 Publication

Chemistry

ChEMBLiCHEMBL1075298.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373P2Y purinoceptor 2PRO_0000070015Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi9 – 91N-linked (GlcNAc...)Sequence analysis
Glycosylationi13 – 131N-linked (GlcNAc...)Sequence analysis
Disulfide bondi106 ↔ 183PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35383.
PaxDbiP35383.
PRIDEiP35383.

PTM databases

PhosphoSiteiP35383.

Expressioni

Tissue specificityi

Spleen, testis, kidney, liver, lung, heart and brain.

Gene expression databases

BgeeiP35383.
CleanExiMM_P2RY2.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036765.

Chemistry

BindingDBiP35383.

Structurei

3D structure databases

ProteinModelPortaliP35383.
SMRiP35383. Positions 23-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIAQ. Eukaryota.
ENOG410XQNZ. LUCA.
HOGENOMiHOG000231307.
HOVERGENiHBG101120.
InParanoidiP35383.
KOiK04269.
OrthoDBiEOG7QNVM4.
PhylomeDBiP35383.
TreeFamiTF350009.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000356. P2Y2_rcpt.
[Graphical view]
PANTHERiPTHR24231:SF17. PTHR24231:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00594. P2Y2PRNOCPTR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADLEPWNS TINGTWEGDE LGYKCRFNED FKYVLLPVSY GVVCVLGLCL
60 70 80 90 100
NVVALYIFLC RLKTWNASTT YMFHLAVSDS LYAASLPLLV YYYARGDHWP
110 120 130 140 150
FSTVLCKLVR FLFYTNLYCS ILFLTCISVH RCLGVLRPLH SLRWGRARYA
160 170 180 190 200
RRVAAVVWVL VLACQAPVLY FVTTSVRGTR ITCHDTSARE LFSHFVAYSS
210 220 230 240 250
VMLGLLFAVP FSVILVCYVL MARRLLKPAY GTTGGLPRAK RKSVRTIALV
260 270 280 290 300
LAVFALCFLP FHVTRTLYYS FRSLDLSCHT LNAINMAYKI TRPLASANSC
310 320 330 340 350
LDPVLYFLAG QRLVRFARDA KPPTEPTPSP QARRKLGLHR PNRTVRKDLS
360 370
VSSDDSRRTE STPAGSETKD IRL
Length:373
Mass (Da):42,175
Last modified:June 1, 1994 - v1
Checksum:i590BBE502E41B3AE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171E → D in AAB50735 (PubMed:8739319).Curated
Sequence conflicti120 – 1201S → R in AAB50735 (PubMed:8739319).Curated
Sequence conflicti125 – 1251T → N in AAB50735 (PubMed:8739319).Curated
Sequence conflicti196 – 1961V → M in AAB50735 (PubMed:8739319).Curated
Sequence conflicti263 – 2631V → L in BAB23746 (PubMed:16141072).Curated
Sequence conflicti263 – 2631V → L in BAB30719 (PubMed:16141072).Curated
Sequence conflicti355 – 3551D → N in AAB50735 (PubMed:8739319).Curated
Sequence conflicti369 – 3713KDI → PYV in AAB50735 (PubMed:8739319).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14751 mRNA. Translation: AAA39871.1.
S83099 mRNA. Translation: AAB50735.1.
AK005013 mRNA. Translation: BAB23746.1.
AK017378 mRNA. Translation: BAB30719.1.
BC006613 mRNA. Translation: AAH06613.1.
CCDSiCCDS21508.1.
PIRiA47556.
RefSeqiNP_001289275.1. NM_001302346.1.
NP_001289276.1. NM_001302347.1.
NP_032799.2. NM_008773.4.
UniGeneiMm.3000.

Genome annotation databases

GeneIDi18442.
KEGGimmu:18442.
UCSCiuc009iny.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14751 mRNA. Translation: AAA39871.1.
S83099 mRNA. Translation: AAB50735.1.
AK005013 mRNA. Translation: BAB23746.1.
AK017378 mRNA. Translation: BAB30719.1.
BC006613 mRNA. Translation: AAH06613.1.
CCDSiCCDS21508.1.
PIRiA47556.
RefSeqiNP_001289275.1. NM_001302346.1.
NP_001289276.1. NM_001302347.1.
NP_032799.2. NM_008773.4.
UniGeneiMm.3000.

3D structure databases

ProteinModelPortaliP35383.
SMRiP35383. Positions 23-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036765.

Chemistry

BindingDBiP35383.
ChEMBLiCHEMBL1075298.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP35383.

Proteomic databases

MaxQBiP35383.
PaxDbiP35383.
PRIDEiP35383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18442.
KEGGimmu:18442.
UCSCiuc009iny.2. mouse.

Organism-specific databases

CTDi5029.
MGIiMGI:105107. P2ry2.

Phylogenomic databases

eggNOGiENOG410IIAQ. Eukaryota.
ENOG410XQNZ. LUCA.
HOGENOMiHOG000231307.
HOVERGENiHBG101120.
InParanoidiP35383.
KOiK04269.
OrthoDBiEOG7QNVM4.
PhylomeDBiP35383.
TreeFamiTF350009.

Miscellaneous databases

ChiTaRSiP2ry2. mouse.
NextBioi294132.
PROiP35383.
SOURCEiSearch...

Gene expression databases

BgeeiP35383.
CleanExiMM_P2RY2.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000356. P2Y2_rcpt.
[Graphical view]
PANTHERiPTHR24231:SF17. PTHR24231:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00594. P2Y2PRNOCPTR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of an ATP receptor from mouse neuroblastoma cells."
    Lustig K.D., Shiau A.K., Brake A.J., Julius D.
    Proc. Natl. Acad. Sci. U.S.A. 90:5113-5117(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells."
    Enomoto K., Furuya K., Moore R.C., Yamagishi S., Oka T., Maeno T.
    Biol. Signals 5:9-21(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  5. "Site-directed mutagenesis of P2U purinoceptors. Positively charged amino acids in transmembrane helices 6 and 7 affect agonist potency and specificity."
    Erb L., Garrad R.C., Wang Y., Quinn T., Turner J.T., Weisman G.A.
    J. Biol. Chem. 270:4185-4188(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-107; ARG-110; HIS-262; ARG-265; LYS-289 AND ARG-292.

Entry informationi

Entry nameiP2RY2_MOUSE
AccessioniPrimary (citable) accession number: P35383
Secondary accession number(s): O09031, Q9CPZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 20, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.