P35374 (AGTR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 angiotensin II receptor

Alternative name(s):
Angiotensin II type-2 receptor
Short name=AT2

Gene names

Name:

Agtr2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation. Ref.8
Subunit structure	Interacts with MTUS1. Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.8.
Tissue specificity	Expressed at highest levels in adrenal gland and uterus. Ref.8
Post-translational modification	C-terminal Ser or Thr residues may be phosphorylated.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAB49539.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Inferred from direct assay PubMed 17000928. Source: BHF-UCL aldosterone secretion Inferred from electronic annotation. Source: Compara behavior Inferred from mutant phenotype PubMed 7477267. Source: BHF-UCL brain renin-angiotensin system Inferred from direct assay PubMed 9878677. Source: MGI cell growth involved in cardiac muscle cell development Inferred from electronic annotation. Source: Compara cellular response to dexamethasone stimulus Inferred from electronic annotation. Source: Compara cellular sodium ion homeostasis Inferred from electronic annotation. Source: Compara cerebellar cortex development Inferred from electronic annotation. Source: Compara dopamine biosynthetic process Inferred from electronic annotation. Source: Compara inflammatory response Inferred from genetic interaction PubMed 19404405. Source: MGI intracellular protein kinase cascade Inferred from direct assay PubMed 17000928. Source: BHF-UCL negative regulation of fibroblast proliferation Inferred from electronic annotation. Source: Compara negative regulation of heart rate Inferred from mutant phenotype PubMed 9449684. Source: BHF-UCL negative regulation of neurotrophin TRK receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of norepinephrine secretion Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of branching involved in ureteric bud morphogenesis Inferred from mutant phenotype PubMed 18607644. Source: UniProtKB positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cytokine secretion Inferred from electronic annotation. Source: Compara positive regulation of metanephric glomerulus development Inferred from mutant phenotype PubMed 18607644. Source: UniProtKB positive regulation of nitric-oxide synthase activity Inferred from direct assay PubMed 17000928. Source: BHF-UCL positive regulation of phosphoprotein phosphatase activity Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 15153556PubMed 18607644. Source: UniProtKB positive regulation of vasodilation Inferred from electronic annotation. Source: Compara regulation of metanephros size Inferred from mutant phenotype PubMed 18607644. Source: UniProtKB regulation of systemic arterial blood pressure by circulatory renin-angiotensin Inferred from mutant phenotype PubMed 7477267. Source: BHF-UCL regulation of transcription factor import into nucleus Inferred from electronic annotation. Source: Compara renin-angiotensin regulation of aldosterone production Inferred from electronic annotation. Source: Compara response to organic nitrogen Inferred from electronic annotation. Source: Compara vasodilation by angiotensin involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype PubMed 10425188. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	angiotensin type II receptor activity Inferred from direct assay PubMed 9878677. Source: MGI peptide hormone binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Type-2 angiotensin II receptor		PRO_0000069169
Regions
	Topological domain	1 – 45	45	Extracellular Potential
	Transmembrane	46 – 71	26	Helical; Name=1; Potential
	Topological domain	72 – 80	9	Cytoplasmic Potential
	Transmembrane	81 – 102	22	Helical; Name=2; Potential
	Topological domain	103 – 119	17	Extracellular Potential
	Transmembrane	120 – 140	21	Helical; Name=3; Potential
	Topological domain	141 – 160	20	Cytoplasmic Potential
	Transmembrane	161 – 179	19	Helical; Name=4; Potential
	Topological domain	180 – 208	29	Extracellular Potential
	Transmembrane	209 – 234	26	Helical; Name=5; Potential
	Topological domain	235 – 256	22	Cytoplasmic Potential
	Transmembrane	257 – 278	22	Helical; Name=6; Potential
	Topological domain	279 – 297	19	Extracellular Potential
	Transmembrane	298 – 318	21	Helical; Name=7; Potential
	Topological domain	319 – 363	45	Cytoplasmic Potential
Amino acid modifications
	Modified residue	354	1	Phosphoserine; by PKC Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	35 ↔ 290		Ref.7
	Disulfide bond	117 ↔ 195		Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P35374 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6C7D6E3B026D1E80	FASTA	363	41,374
	10 20 30 40 50 60 MKDNFSFAAT SRNITSSRPF DNLNATGTNE SAFNCSHKPS DKHLEAIPVL YYMIFVIGFA 70 80 90 100 110 120 VNIVVVSLFC CQKGPKKVSS IYIFNLALAD LLLLATLPLW ATYYSYRYDW LFGPVMCKVF 130 140 150 160 170 180 GSFLTLNMFA SIFFITCMSV DRYQSVIYPF LSQRRNPWQA SYVVPLVWCM ACLSSLPTFY 190 200 210 220 230 240 FRDVRTIEYL GVNACIMAFP PEKYAQWSAG IALMKNILGF IIPLIFIATC YFGIRKHLLK 250 260 270 280 290 300 TNSYGKNRIT RDQVLKMAAA VVLAFIICWL PFHVLTFLDA LTWMGIINSC EVIAVIDLAL 310 320 330 340 350 360 PFAILLGFTN SCVNPFLYCF VGNRFQQKLR SVFRVPITWL QGKRETMSCR KGSSLREMDT FVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNA and analysis of the gene for mouse angiotensin II type 2 receptor." Nakajima M., Mukoyama M., Pratt R.E., Horiuchi M., Dzau V.J. Biochem. Biophys. Res. Commun. 197:393-399(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Fetus.
[2]	"Cloning of the cDNA and the genomic DNA of the mouse angiotensin II type 2 receptor." Ichiki T., Herold C.L., Kambayashi Y., Bardhan S., Inagami T. Biochim. Biophys. Acta 1189:247-250(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[3]	"Molecular and functional characterization of angiotensin II AT2 receptor in neuroblastoma N1E-115 cells." Nahmias C., Cazaubon S.M., Sutren M., Masson M., Lazard D., Villageois P., Elbaz N., Strosberg A.D. Adv. Exp. Med. Biol. 396:167-173(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The growth-dependent expression of angiotensin II type 2 receptor is regulated by transcription factors interferon regulatory factor-1 and -2." Horiuchi M., Koike G., Yamada T., Mukoyama M., Nakajima M., Dzau V.J. J. Biol. Chem. 270:20225-20230(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[7]	"Identification and function of disulfide bridges in the extracellular domains of the angiotensin II type 2 receptor." Heerding J.N., Hines J., Fluharty S.J., Yee D.K. Biochemistry 40:8369-8377(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
[8]	"Regulation of transport of the angiotensin AT2 receptor by a novel membrane-associated Golgi protein." Wruck C.J., Funke-Kaiser H., Pufe T., Kusserow H., Menk M., Schefe J.H., Kruse M.L., Stoll M., Unger T. Arterioscler. Thromb. Vasc. Biol. 25:57-64(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MTUS1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S67465 mRNA. Translation: AAB29336.1. U04828 mRNA. Translation: AAC52128.1. U00766 mRNA. Translation: AAC04933.1. L32840 mRNA. Translation: AAB49539.1. Different initiation. U11073 Genomic DNA. Translation: AAA82184.1. AK086334 mRNA. Translation: BAC39650.1. BC003811 mRNA. Translation: AAH03811.1.
IPI	IPI00985658.
PIR	I48261.
RefSeq	NP_031455.1. NM_007429.5.
UniGene	Mm.2679.
3D structure databases
ProteinModelPortal	P35374.
SMR	P35374. Positions 46-330.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35374.
Proteomic databases
PRIDE	P35374.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000089188; ENSMUSP00000086592; ENSMUSG00000068122.
GeneID	11609.
KEGG	mmu:11609.
UCSC	uc009suq.2. mouse.
Organism-specific databases
CTD	186.
MGI	MGI:87966. Agtr2.
Phylogenomic databases
eggNOG	NOG311643.
GeneTree	ENSGT00700000104167.
HOVERGEN	HBG104998.
InParanoid	P35374.
KO	K04167.
OrthoDB	EOG4SXND6.
Gene expression databases
ArrayExpress	P35374.
Bgee	P35374.
CleanEx	MM_AGTR2.
Genevestigator	P35374.
GermOnline	ENSMUSG00000068122. Mus musculus.
Family and domain databases
InterPro	IPR000147. ATII_AT2_rcpt. IPR000248. ATII_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00241. ANGIOTENSINR. PR00636. ANGIOTENSN2R. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279152.
SOURCE	Search...

Entry information

Entry name

AGTR2_MOUSE

Accession

Primary (citable) accession number: P35374

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program