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P35370

- OPRX_RAT

UniProt

P35370 - OPRX_RAT

Protein

Nociceptin receptor

Gene

Oprl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei107 – 1071Important for G protein-mediated signalingBy similarity
    Sitei127 – 1271Important for G protein-mediated signalingBy similarity

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB
    2. nociceptin receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
    2. behavior Source: UniProtKB-KW
    3. opioid receptor signaling pathway Source: GOC
    4. positive regulation of cytosolic calcium ion concentration Source: Ensembl
    5. sensory perception of pain Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nociceptin receptor
    Alternative name(s):
    Kappa-type 3 opioid receptor
    Short name:
    KOR-3
    Orphanin FQ receptor
    ROR-C
    XOR1
    Gene namesi
    Name:Oprl1
    Synonyms:Oor, Oprl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi68438. Oprl1.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle By similarity
    Note: Ligand binding leads to receptor internalization into cytoplasmic vesicles, decreasing the amount of available receptor at the cell surface By similarity. Internalization requires phosphorylation at Ser-360. Can recycle to the cell membrane.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    2. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Nociceptin receptorPRO_0000069983Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi120 ↔ 197PROSITE-ProRule annotation
    Lipidationi331 – 3311S-palmitoyl cysteineSequence Analysis

    Post-translational modificationi

    Phosphorylation at Ser-360 requires ADRBK2.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    PTM databases

    PhosphoSiteiP35370.

    Expressioni

    Tissue specificityi

    Highly expressed in several brain areas, the intestine, liver and spleen. Detected in sympathetic stellate ganglion neurons.7 Publications

    Gene expression databases

    GenevestigatoriP35370.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP35370.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4545ExtracellularBy similarityAdd
    BLAST
    Topological domaini72 – 8413CytoplasmicBy similarityAdd
    BLAST
    Topological domaini107 – 12115ExtracellularBy similarityAdd
    BLAST
    Topological domaini144 – 16219CytoplasmicBy similarityAdd
    BLAST
    Topological domaini186 – 20823ExtracellularBy similarityAdd
    BLAST
    Topological domaini234 – 26128CytoplasmicBy similarityAdd
    BLAST
    Topological domaini283 – 29715ExtracellularBy similarityAdd
    BLAST
    Topological domaini320 – 36748CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei46 – 7126Helical; Name=1By similarityAdd
    BLAST
    Transmembranei85 – 10622Helical; Name=2By similarityAdd
    BLAST
    Transmembranei122 – 14322Helical; Name=3By similarityAdd
    BLAST
    Transmembranei163 – 18523Helical; Name=4By similarityAdd
    BLAST
    Transmembranei209 – 23325Helical; Name=5By similarityAdd
    BLAST
    Transmembranei262 – 28221Helical; Name=6By similarityAdd
    BLAST
    Transmembranei298 – 31922Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG299442.
    GeneTreeiENSGT00630000089574.
    HOGENOMiHOG000230486.
    HOVERGENiHBG106919.
    InParanoidiP35370.
    KOiK04216.
    OMAiVFAICIF.
    OrthoDBiEOG7BKCVQ.
    PhylomeDBiP35370.
    TreeFamiTF315737.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001418. Opioid_rcpt.
    IPR001420. X_opioid_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00384. OPIOIDR.
    PR00547. XOPIOIDR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35370-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLFPAPYW EVLYGSHFQG NLSLLNETVP HHLLLNASHS AFLPLGLKVT    50
    IVGLYLAVCI GGLLGNCLVM YVILRHTKMK TATNIYIFNL ALADTLVLLT 100
    LPFQGTDILL GFWPFGNALC KTVIAIDYYN MFTSTFTLTA MSVDRYVAIC 150
    HPIRALDVRT SSKAQAVNVA IWALASVVGV PVAIMGSAQV EDEEIECLVE 200
    IPAPQDYWGP VFAICIFLFS FIIPVLIISV CYSLMIRRLR GVRLLSGSRE 250
    KDRNLRRITR LVLVVVAVFV GCWTPVQVFV LVQGLGVQPG SETAVAILRF 300
    CTALGYVNSC LNPILYAFLD ENFKACFRKF CCASSLHREM QVSDRVRSIA 350
    KDVGLGCKTS ETVPRPA 367
    Length:367
    Mass (Da):40,523
    Last modified:June 1, 1994 - v1
    Checksum:iEB2637582747B4AD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1051G → R in AAA16201. 1 PublicationCurated
    Sequence conflicti226 – 2261L → V in AAA16201. 1 PublicationCurated
    Sequence conflicti246 – 2461S → P in AAA16201. 1 PublicationCurated
    Sequence conflicti348 – 3481S → T(PubMed:8034019)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16438 mRNA. Translation: BAA03908.1.
    U05239 mRNA. Translation: AAA16201.1.
    U01913 mRNA. Translation: AAA21025.1.
    L28144 mRNA. Translation: AAC37661.1.
    U07871 mRNA. Translation: AAA69927.1.
    L33916 mRNA. Translation: AAA50827.1.
    L29419 mRNA. Translation: AAC42041.1.
    AF216218 Genomic DNA. Translation: AAF80990.1.
    AY152731 mRNA. Translation: AAN77720.1.
    PIRiI56520.
    RefSeqiNP_113757.1. NM_031569.3.
    XP_006235798.1. XM_006235736.1.
    XP_006235799.1. XM_006235737.1.
    XP_006235800.1. XM_006235738.1.
    UniGeneiRn.9762.

    Genome annotation databases

    EnsembliENSRNOT00000045845; ENSRNOP00000047053; ENSRNOG00000016768.
    GeneIDi29256.
    KEGGirno:29256.
    UCSCiRGD:68438. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16438 mRNA. Translation: BAA03908.1 .
    U05239 mRNA. Translation: AAA16201.1 .
    U01913 mRNA. Translation: AAA21025.1 .
    L28144 mRNA. Translation: AAC37661.1 .
    U07871 mRNA. Translation: AAA69927.1 .
    L33916 mRNA. Translation: AAA50827.1 .
    L29419 mRNA. Translation: AAC42041.1 .
    AF216218 Genomic DNA. Translation: AAF80990.1 .
    AY152731 mRNA. Translation: AAN77720.1 .
    PIRi I56520.
    RefSeqi NP_113757.1. NM_031569.3.
    XP_006235798.1. XM_006235736.1.
    XP_006235799.1. XM_006235737.1.
    XP_006235800.1. XM_006235738.1.
    UniGenei Rn.9762.

    3D structure databases

    ProteinModelPortali P35370.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P35370.
    ChEMBLi CHEMBL4503.
    GuidetoPHARMACOLOGYi 320.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P35370.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000045845 ; ENSRNOP00000047053 ; ENSRNOG00000016768 .
    GeneIDi 29256.
    KEGGi rno:29256.
    UCSCi RGD:68438. rat.

    Organism-specific databases

    CTDi 4987.
    RGDi 68438. Oprl1.

    Phylogenomic databases

    eggNOGi NOG299442.
    GeneTreei ENSGT00630000089574.
    HOGENOMi HOG000230486.
    HOVERGENi HBG106919.
    InParanoidi P35370.
    KOi K04216.
    OMAi VFAICIF.
    OrthoDBi EOG7BKCVQ.
    PhylomeDBi P35370.
    TreeFami TF315737.

    Miscellaneous databases

    NextBioi 608564.
    PROi P35370.

    Gene expression databases

    Genevestigatori P35370.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001418. Opioid_rcpt.
    IPR001420. X_opioid_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00384. OPIOIDR.
    PR00547. XOPIOIDR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and regional distribution of a novel member of the opioid receptor family."
      Fukuda K., Kato S., Mori K., Nishi M., Takeshima H., Iwabe N., Miyata T., Houtani T., Sugimoto T.
      FEBS Lett. 343:42-46(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Wistar.
      Tissue: Brain.
    2. Meng F., Xie G., Alfred M., Thompson R., Hoversten M., Watson S., Akil H.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hippocampus.
    3. "Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a mu, delta or kappa opioid receptor type."
      Bunzow J.R., Saez C., Mortrud M., Bouvier C., Williams J.T., Low M., Grandy D.K.
      FEBS Lett. 347:284-288(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Molecular cloning, tissue distribution and chromosomal localization of a novel member of the opioid receptor gene family."
      Chen Y., Fan Y., Liu J., Mestek A., Tian M., Kozak C.A., Yu L.
      FEBS Lett. 347:279-283(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    5. "Molecular cloning of a novel G protein-coupled receptor related to the opiate receptor family."
      Lachowicz J.E., Shen Y., Monsma F.J. Jr., Sibley D.R.
      J. Neurochem. 64:34-40(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Brain.
    6. "cDNA cloning of an orphan opiate receptor gene family member and its splice variant."
      Wang J.B., Johnson P.S., Imai Y., Persico A.M., Ozenberger B.A., Eppler C.M., Uhl G.R.
      FEBS Lett. 348:75-79(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Brain.
    7. "Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned mu, delta, and kappa opioid receptors."
      Wick M.J., Minnerath S.R., Lin X., Elde R.P., Law P.Y., Loh H.H.
      Brain Res. Mol. Brain Res. 27:37-44(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    8. "Molecular cloning of the orphanin FQ receptor gene and differential tissue expression of splice variants in rat."
      Curro D., Song I., Anderson M., Yoo J.H., Del Valle J., Owyang C.
      Gene 266:139-145(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    9. "Modulation of Ca2+ channels by opioid receptor-like 1 receptors natively expressed in rat stellate ganglion neurons innervating cardiac muscle."
      Ruiz-Velasco V., Puhl H.L., Fuller B.C., Sumner A.D.
      J. Pharmacol. Exp. Ther. 314:987-994(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: Wistar.
    10. "Involvement of ORL1 receptor and ERK kinase in the orphanin FQ-induced nociception in the nucleus accumbens of rats."
      Chen L.Y., Huang J.X., Yu L.C.
      Regul. Pept. 151:43-47(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiOPRX_RAT
    AccessioniPrimary (citable) accession number: P35370
    Secondary accession number(s): Q791R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3