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Protein

Nociceptin receptor

Gene

Oprl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107Important for G protein-mediated signalingBy similarity1
Sitei127Important for G protein-mediated signalingBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  • chemical synaptic transmission Source: GO_Central
  • eating behavior Source: RGD
  • estrous cycle Source: RGD
  • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
  • negative regulation of blood pressure Source: RGD
  • negative regulation of cAMP biosynthetic process Source: RGD
  • negative regulation of voltage-gated calcium channel activity Source: RGD
  • neuropeptide signaling pathway Source: GO_Central
  • opioid receptor signaling pathway Source: GO_Central
  • positive regulation of cytosolic calcium ion concentration Source: Ensembl
  • positive regulation of gastric acid secretion Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of sensory perception of pain Source: RGD
  • positive regulation of urine volume Source: RGD
  • regulation of cAMP biosynthetic process Source: RGD
  • regulation of locomotor rhythm Source: RGD
  • regulation of sensory perception of pain Source: RGD
  • response to estradiol Source: RGD
  • sensory perception of pain Source: UniProtKB

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Nociceptin receptor
Alternative name(s):
Kappa-type 3 opioid receptor
Short name:
KOR-3
Orphanin FQ receptor
ROR-C
XOR1
Gene namesi
Name:Oprl1
Synonyms:Oor, Oprl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi68438. Oprl1.

Subcellular locationi

  • Cell membrane; Multi-pass membrane protein
  • Cytoplasmic vesicle By similarity

  • Note: Ligand binding leads to receptor internalization into cytoplasmic vesicles, decreasing the amount of available receptor at the cell surface (By similarity). Internalization requires phosphorylation at Ser-360. Can recycle to the cell membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45ExtracellularBy similarityAdd BLAST45
Transmembranei46 – 71Helical; Name=1By similarityAdd BLAST26
Topological domaini72 – 84CytoplasmicBy similarityAdd BLAST13
Transmembranei85 – 106Helical; Name=2By similarityAdd BLAST22
Topological domaini107 – 121ExtracellularBy similarityAdd BLAST15
Transmembranei122 – 143Helical; Name=3By similarityAdd BLAST22
Topological domaini144 – 162CytoplasmicBy similarityAdd BLAST19
Transmembranei163 – 185Helical; Name=4By similarityAdd BLAST23
Topological domaini186 – 208ExtracellularBy similarityAdd BLAST23
Transmembranei209 – 233Helical; Name=5By similarityAdd BLAST25
Topological domaini234 – 261CytoplasmicBy similarityAdd BLAST28
Transmembranei262 – 282Helical; Name=6By similarityAdd BLAST21
Topological domaini283 – 297ExtracellularBy similarityAdd BLAST15
Transmembranei298 – 319Helical; Name=7By similarityAdd BLAST22
Topological domaini320 – 367CytoplasmicBy similarityAdd BLAST48

GO - Cellular componenti

  • cytoplasmic vesicle Source: UniProtKB-KW
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: RGD
  • neuron projection Source: GO_Central

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4503.
GuidetoPHARMACOLOGYi320.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699831 – 367Nociceptin receptorAdd BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi26N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi36N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi120 ↔ 197PROSITE-ProRule annotation
Lipidationi331S-palmitoyl cysteineSequence analysis1

Post-translational modificationi

Phosphorylation at Ser-360 requires GRK3.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP35370.

PTM databases

iPTMnetiP35370.
PhosphoSitePlusiP35370.

Expressioni

Tissue specificityi

Highly expressed in several brain areas, the intestine, liver and spleen. Detected in sympathetic stellate ganglion neurons.7 Publications

Gene expression databases

BgeeiENSRNOG00000016768.
ExpressionAtlasiP35370. baseline.
GenevisibleiP35370. RN.

Interactioni

GO - Molecular functioni

  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000047053.

Chemistry databases

BindingDBiP35370.

Structurei

3D structure databases

ProteinModelPortaliP35370.
SMRiP35370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP35370.
KOiK04216.
OMAiRTPHKAK.
OrthoDBiEOG091G0HEN.
PhylomeDBiP35370.
TreeFamiTF315737.

Family and domain databases

CDDicd15092. 7tmA_NOFQ_opioid_R. 1 hit.
InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P35370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLFPAPYW EVLYGSHFQG NLSLLNETVP HHLLLNASHS AFLPLGLKVT
60 70 80 90 100
IVGLYLAVCI GGLLGNCLVM YVILRHTKMK TATNIYIFNL ALADTLVLLT
110 120 130 140 150
LPFQGTDILL GFWPFGNALC KTVIAIDYYN MFTSTFTLTA MSVDRYVAIC
160 170 180 190 200
HPIRALDVRT SSKAQAVNVA IWALASVVGV PVAIMGSAQV EDEEIECLVE
210 220 230 240 250
IPAPQDYWGP VFAICIFLFS FIIPVLIISV CYSLMIRRLR GVRLLSGSRE
260 270 280 290 300
KDRNLRRITR LVLVVVAVFV GCWTPVQVFV LVQGLGVQPG SETAVAILRF
310 320 330 340 350
CTALGYVNSC LNPILYAFLD ENFKACFRKF CCASSLHREM QVSDRVRSIA
360
KDVGLGCKTS ETVPRPA
Length:367
Mass (Da):40,523
Last modified:June 1, 1994 - v1
Checksum:iEB2637582747B4AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105G → R in AAA16201 (Ref. 2) Curated1
Sequence conflicti226L → V in AAA16201 (Ref. 2) Curated1
Sequence conflicti246S → P in AAA16201 (Ref. 2) Curated1
Sequence conflicti348S → T (PubMed:8034019).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16438 mRNA. Translation: BAA03908.1.
U05239 mRNA. Translation: AAA16201.1.
U01913 mRNA. Translation: AAA21025.1.
L28144 mRNA. Translation: AAC37661.1.
U07871 mRNA. Translation: AAA69927.1.
L33916 mRNA. Translation: AAA50827.1.
L29419 mRNA. Translation: AAC42041.1.
AF216218 Genomic DNA. Translation: AAF80990.1.
AY152731 mRNA. Translation: AAN77720.1.
PIRiI56520.
RefSeqiNP_113757.1. NM_031569.4.
XP_006235798.1. XM_006235736.3.
XP_006235800.1. XM_006235738.3.
XP_017447001.1. XM_017591512.1.
XP_017447002.1. XM_017591513.1.
XP_017447003.1. XM_017591514.1.
UniGeneiRn.9762.

Genome annotation databases

EnsembliENSRNOT00000045845; ENSRNOP00000047053; ENSRNOG00000016768.
GeneIDi29256.
KEGGirno:29256.
UCSCiRGD:68438. rat.

Entry informationi

Entry nameiOPRX_RAT
AccessioniPrimary (citable) accession number: P35370
Secondary accession number(s): Q791R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: August 30, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families