Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P35370

- OPRX_RAT

UniProt

P35370 - OPRX_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Nociceptin receptor

Gene

Oprl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei107 – 1071Important for G protein-mediated signalingBy similarity
Sitei127 – 1271Important for G protein-mediated signalingBy similarity

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB
  2. nociceptin receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  2. behavior Source: UniProtKB-KW
  3. opioid receptor signaling pathway Source: GOC
  4. positive regulation of cytosolic calcium ion concentration Source: Ensembl
  5. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
Nociceptin receptor
Alternative name(s):
Kappa-type 3 opioid receptor
Short name:
KOR-3
Orphanin FQ receptor
ROR-C
XOR1
Gene namesi
Name:Oprl1
Synonyms:Oor, Oprl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi68438. Oprl1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle By similarity
Note: Ligand binding leads to receptor internalization into cytoplasmic vesicles, decreasing the amount of available receptor at the cell surface (By similarity). Internalization requires phosphorylation at Ser-360. Can recycle to the cell membrane.By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Nociceptin receptorPRO_0000069983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi120 ↔ 197PROSITE-ProRule annotation
Lipidationi331 – 3311S-palmitoyl cysteineSequence Analysis

Post-translational modificationi

Phosphorylation at Ser-360 requires ADRBK2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

PTM databases

PhosphoSiteiP35370.

Expressioni

Tissue specificityi

Highly expressed in several brain areas, the intestine, liver and spleen. Detected in sympathetic stellate ganglion neurons.7 Publications

Gene expression databases

ExpressionAtlasiP35370. baseline.
GenevestigatoriP35370.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP35370.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularBy similarityAdd
BLAST
Topological domaini72 – 8413CytoplasmicBy similarityAdd
BLAST
Topological domaini107 – 12115ExtracellularBy similarityAdd
BLAST
Topological domaini144 – 16219CytoplasmicBy similarityAdd
BLAST
Topological domaini186 – 20823ExtracellularBy similarityAdd
BLAST
Topological domaini234 – 26128CytoplasmicBy similarityAdd
BLAST
Topological domaini283 – 29715ExtracellularBy similarityAdd
BLAST
Topological domaini320 – 36748CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei46 – 7126Helical; Name=1By similarityAdd
BLAST
Transmembranei85 – 10622Helical; Name=2By similarityAdd
BLAST
Transmembranei122 – 14322Helical; Name=3By similarityAdd
BLAST
Transmembranei163 – 18523Helical; Name=4By similarityAdd
BLAST
Transmembranei209 – 23325Helical; Name=5By similarityAdd
BLAST
Transmembranei262 – 28221Helical; Name=6By similarityAdd
BLAST
Transmembranei298 – 31922Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG299442.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP35370.
KOiK04216.
OMAiVFAICIF.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP35370.
TreeFamiTF315737.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35370-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESLFPAPYW EVLYGSHFQG NLSLLNETVP HHLLLNASHS AFLPLGLKVT
60 70 80 90 100
IVGLYLAVCI GGLLGNCLVM YVILRHTKMK TATNIYIFNL ALADTLVLLT
110 120 130 140 150
LPFQGTDILL GFWPFGNALC KTVIAIDYYN MFTSTFTLTA MSVDRYVAIC
160 170 180 190 200
HPIRALDVRT SSKAQAVNVA IWALASVVGV PVAIMGSAQV EDEEIECLVE
210 220 230 240 250
IPAPQDYWGP VFAICIFLFS FIIPVLIISV CYSLMIRRLR GVRLLSGSRE
260 270 280 290 300
KDRNLRRITR LVLVVVAVFV GCWTPVQVFV LVQGLGVQPG SETAVAILRF
310 320 330 340 350
CTALGYVNSC LNPILYAFLD ENFKACFRKF CCASSLHREM QVSDRVRSIA
360
KDVGLGCKTS ETVPRPA
Length:367
Mass (Da):40,523
Last modified:June 1, 1994 - v1
Checksum:iEB2637582747B4AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051G → R in AAA16201. 1 PublicationCurated
Sequence conflicti226 – 2261L → V in AAA16201. 1 PublicationCurated
Sequence conflicti246 – 2461S → P in AAA16201. 1 PublicationCurated
Sequence conflicti348 – 3481S → T(PubMed:8034019)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16438 mRNA. Translation: BAA03908.1.
U05239 mRNA. Translation: AAA16201.1.
U01913 mRNA. Translation: AAA21025.1.
L28144 mRNA. Translation: AAC37661.1.
U07871 mRNA. Translation: AAA69927.1.
L33916 mRNA. Translation: AAA50827.1.
L29419 mRNA. Translation: AAC42041.1.
AF216218 Genomic DNA. Translation: AAF80990.1.
AY152731 mRNA. Translation: AAN77720.1.
PIRiI56520.
RefSeqiNP_113757.1. NM_031569.3.
XP_006235798.1. XM_006235736.2.
XP_006235800.1. XM_006235738.2.
UniGeneiRn.9762.

Genome annotation databases

EnsembliENSRNOT00000045845; ENSRNOP00000047053; ENSRNOG00000016768.
GeneIDi29256.
KEGGirno:29256.
UCSCiRGD:68438. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16438 mRNA. Translation: BAA03908.1 .
U05239 mRNA. Translation: AAA16201.1 .
U01913 mRNA. Translation: AAA21025.1 .
L28144 mRNA. Translation: AAC37661.1 .
U07871 mRNA. Translation: AAA69927.1 .
L33916 mRNA. Translation: AAA50827.1 .
L29419 mRNA. Translation: AAC42041.1 .
AF216218 Genomic DNA. Translation: AAF80990.1 .
AY152731 mRNA. Translation: AAN77720.1 .
PIRi I56520.
RefSeqi NP_113757.1. NM_031569.3.
XP_006235798.1. XM_006235736.2.
XP_006235800.1. XM_006235738.2.
UniGenei Rn.9762.

3D structure databases

ProteinModelPortali P35370.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P35370.
ChEMBLi CHEMBL4503.
GuidetoPHARMACOLOGYi 320.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P35370.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000045845 ; ENSRNOP00000047053 ; ENSRNOG00000016768 .
GeneIDi 29256.
KEGGi rno:29256.
UCSCi RGD:68438. rat.

Organism-specific databases

CTDi 4987.
RGDi 68438. Oprl1.

Phylogenomic databases

eggNOGi NOG299442.
GeneTreei ENSGT00760000118797.
HOGENOMi HOG000230486.
HOVERGENi HBG106919.
InParanoidi P35370.
KOi K04216.
OMAi VFAICIF.
OrthoDBi EOG7BKCVQ.
PhylomeDBi P35370.
TreeFami TF315737.

Miscellaneous databases

NextBioi 608564.
PROi P35370.

Gene expression databases

ExpressionAtlasi P35370. baseline.
Genevestigatori P35370.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and regional distribution of a novel member of the opioid receptor family."
    Fukuda K., Kato S., Mori K., Nishi M., Takeshima H., Iwabe N., Miyata T., Houtani T., Sugimoto T.
    FEBS Lett. 343:42-46(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  2. Meng F., Xie G., Alfred M., Thompson R., Hoversten M., Watson S., Akil H.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hippocampus.
  3. "Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a mu, delta or kappa opioid receptor type."
    Bunzow J.R., Saez C., Mortrud M., Bouvier C., Williams J.T., Low M., Grandy D.K.
    FEBS Lett. 347:284-288(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Molecular cloning, tissue distribution and chromosomal localization of a novel member of the opioid receptor gene family."
    Chen Y., Fan Y., Liu J., Mestek A., Tian M., Kozak C.A., Yu L.
    FEBS Lett. 347:279-283(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  5. "Molecular cloning of a novel G protein-coupled receptor related to the opiate receptor family."
    Lachowicz J.E., Shen Y., Monsma F.J. Jr., Sibley D.R.
    J. Neurochem. 64:34-40(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  6. "cDNA cloning of an orphan opiate receptor gene family member and its splice variant."
    Wang J.B., Johnson P.S., Imai Y., Persico A.M., Ozenberger B.A., Eppler C.M., Uhl G.R.
    FEBS Lett. 348:75-79(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  7. "Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned mu, delta, and kappa opioid receptors."
    Wick M.J., Minnerath S.R., Lin X., Elde R.P., Law P.Y., Loh H.H.
    Brain Res. Mol. Brain Res. 27:37-44(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  8. "Molecular cloning of the orphanin FQ receptor gene and differential tissue expression of splice variants in rat."
    Curro D., Song I., Anderson M., Yoo J.H., Del Valle J., Owyang C.
    Gene 266:139-145(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  9. "Modulation of Ca2+ channels by opioid receptor-like 1 receptors natively expressed in rat stellate ganglion neurons innervating cardiac muscle."
    Ruiz-Velasco V., Puhl H.L., Fuller B.C., Sumner A.D.
    J. Pharmacol. Exp. Ther. 314:987-994(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Wistar.
  10. "Involvement of ORL1 receptor and ERK kinase in the orphanin FQ-induced nociception in the nucleus accumbens of rats."
    Chen L.Y., Huang J.X., Yu L.C.
    Regul. Pept. 151:43-47(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiOPRX_RAT
AccessioniPrimary (citable) accession number: P35370
Secondary accession number(s): Q791R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3