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Protein

Nociceptin receptor

Gene

Oprl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei107 – 1071Important for G protein-mediated signalingBy similarity
Sitei127 – 1271Important for G protein-mediated signalingBy similarity

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB
  2. neuropeptide binding Source: GO_Central
  3. nociceptin receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  2. behavior Source: UniProtKB-KW
  3. G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
  4. neuropeptide signaling pathway Source: GO_Central
  5. opioid receptor signaling pathway Source: GO_Central
  6. positive regulation of cytosolic calcium ion concentration Source: Ensembl
  7. sensory perception of pain Source: UniProtKB
  8. synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiREACT_233455. G alpha (i) signalling events.
REACT_250028. Peptide ligand-binding receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Nociceptin receptor
Alternative name(s):
Kappa-type 3 opioid receptor
Short name:
KOR-3
Orphanin FQ receptor
ROR-C
XOR1
Gene namesi
Name:Oprl1
Synonyms:Oor, Oprl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi68438. Oprl1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle By similarity
Note: Ligand binding leads to receptor internalization into cytoplasmic vesicles, decreasing the amount of available receptor at the cell surface (By similarity). Internalization requires phosphorylation at Ser-360. Can recycle to the cell membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularBy similarityAdd
BLAST
Transmembranei46 – 7126Helical; Name=1By similarityAdd
BLAST
Topological domaini72 – 8413CytoplasmicBy similarityAdd
BLAST
Transmembranei85 – 10622Helical; Name=2By similarityAdd
BLAST
Topological domaini107 – 12115ExtracellularBy similarityAdd
BLAST
Transmembranei122 – 14322Helical; Name=3By similarityAdd
BLAST
Topological domaini144 – 16219CytoplasmicBy similarityAdd
BLAST
Transmembranei163 – 18523Helical; Name=4By similarityAdd
BLAST
Topological domaini186 – 20823ExtracellularBy similarityAdd
BLAST
Transmembranei209 – 23325Helical; Name=5By similarityAdd
BLAST
Topological domaini234 – 26128CytoplasmicBy similarityAdd
BLAST
Transmembranei262 – 28221Helical; Name=6By similarityAdd
BLAST
Topological domaini283 – 29715ExtracellularBy similarityAdd
BLAST
Transmembranei298 – 31922Helical; Name=7By similarityAdd
BLAST
Topological domaini320 – 36748CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. integral component of plasma membrane Source: UniProtKB
  3. neuron projection Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Nociceptin receptorPRO_0000069983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi120 ↔ 197PROSITE-ProRule annotation
Lipidationi331 – 3311S-palmitoyl cysteineSequence Analysis

Post-translational modificationi

Phosphorylation at Ser-360 requires ADRBK2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

PTM databases

PhosphoSiteiP35370.

Expressioni

Tissue specificityi

Highly expressed in several brain areas, the intestine, liver and spleen. Detected in sympathetic stellate ganglion neurons.7 Publications

Gene expression databases

ExpressionAtlasiP35370. baseline.
GenevestigatoriP35370.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP35370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG299442.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP35370.
KOiK04216.
OMAiVFAICIF.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP35370.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLFPAPYW EVLYGSHFQG NLSLLNETVP HHLLLNASHS AFLPLGLKVT
60 70 80 90 100
IVGLYLAVCI GGLLGNCLVM YVILRHTKMK TATNIYIFNL ALADTLVLLT
110 120 130 140 150
LPFQGTDILL GFWPFGNALC KTVIAIDYYN MFTSTFTLTA MSVDRYVAIC
160 170 180 190 200
HPIRALDVRT SSKAQAVNVA IWALASVVGV PVAIMGSAQV EDEEIECLVE
210 220 230 240 250
IPAPQDYWGP VFAICIFLFS FIIPVLIISV CYSLMIRRLR GVRLLSGSRE
260 270 280 290 300
KDRNLRRITR LVLVVVAVFV GCWTPVQVFV LVQGLGVQPG SETAVAILRF
310 320 330 340 350
CTALGYVNSC LNPILYAFLD ENFKACFRKF CCASSLHREM QVSDRVRSIA
360
KDVGLGCKTS ETVPRPA
Length:367
Mass (Da):40,523
Last modified:June 1, 1994 - v1
Checksum:iEB2637582747B4AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051G → R in AAA16201 (Ref. 2) Curated
Sequence conflicti226 – 2261L → V in AAA16201 (Ref. 2) Curated
Sequence conflicti246 – 2461S → P in AAA16201 (Ref. 2) Curated
Sequence conflicti348 – 3481S → T (PubMed:8034019).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16438 mRNA. Translation: BAA03908.1.
U05239 mRNA. Translation: AAA16201.1.
U01913 mRNA. Translation: AAA21025.1.
L28144 mRNA. Translation: AAC37661.1.
U07871 mRNA. Translation: AAA69927.1.
L33916 mRNA. Translation: AAA50827.1.
L29419 mRNA. Translation: AAC42041.1.
AF216218 Genomic DNA. Translation: AAF80990.1.
AY152731 mRNA. Translation: AAN77720.1.
PIRiI56520.
RefSeqiNP_113757.1. NM_031569.3.
XP_006235798.1. XM_006235736.2.
XP_006235800.1. XM_006235738.2.
UniGeneiRn.9762.

Genome annotation databases

EnsembliENSRNOT00000045845; ENSRNOP00000047053; ENSRNOG00000016768.
GeneIDi29256.
KEGGirno:29256.
UCSCiRGD:68438. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16438 mRNA. Translation: BAA03908.1.
U05239 mRNA. Translation: AAA16201.1.
U01913 mRNA. Translation: AAA21025.1.
L28144 mRNA. Translation: AAC37661.1.
U07871 mRNA. Translation: AAA69927.1.
L33916 mRNA. Translation: AAA50827.1.
L29419 mRNA. Translation: AAC42041.1.
AF216218 Genomic DNA. Translation: AAF80990.1.
AY152731 mRNA. Translation: AAN77720.1.
PIRiI56520.
RefSeqiNP_113757.1. NM_031569.3.
XP_006235798.1. XM_006235736.2.
XP_006235800.1. XM_006235738.2.
UniGeneiRn.9762.

3D structure databases

ProteinModelPortaliP35370.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP35370.
ChEMBLiCHEMBL4503.
GuidetoPHARMACOLOGYi320.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP35370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000045845; ENSRNOP00000047053; ENSRNOG00000016768.
GeneIDi29256.
KEGGirno:29256.
UCSCiRGD:68438. rat.

Organism-specific databases

CTDi4987.
RGDi68438. Oprl1.

Phylogenomic databases

eggNOGiNOG299442.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP35370.
KOiK04216.
OMAiVFAICIF.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP35370.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiREACT_233455. G alpha (i) signalling events.
REACT_250028. Peptide ligand-binding receptors.

Miscellaneous databases

NextBioi608564.
PROiP35370.

Gene expression databases

ExpressionAtlasiP35370. baseline.
GenevestigatoriP35370.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and regional distribution of a novel member of the opioid receptor family."
    Fukuda K., Kato S., Mori K., Nishi M., Takeshima H., Iwabe N., Miyata T., Houtani T., Sugimoto T.
    FEBS Lett. 343:42-46(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  2. Meng F., Xie G., Alfred M., Thompson R., Hoversten M., Watson S., Akil H.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hippocampus.
  3. "Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a mu, delta or kappa opioid receptor type."
    Bunzow J.R., Saez C., Mortrud M., Bouvier C., Williams J.T., Low M., Grandy D.K.
    FEBS Lett. 347:284-288(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Molecular cloning, tissue distribution and chromosomal localization of a novel member of the opioid receptor gene family."
    Chen Y., Fan Y., Liu J., Mestek A., Tian M., Kozak C.A., Yu L.
    FEBS Lett. 347:279-283(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  5. "Molecular cloning of a novel G protein-coupled receptor related to the opiate receptor family."
    Lachowicz J.E., Shen Y., Monsma F.J. Jr., Sibley D.R.
    J. Neurochem. 64:34-40(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  6. "cDNA cloning of an orphan opiate receptor gene family member and its splice variant."
    Wang J.B., Johnson P.S., Imai Y., Persico A.M., Ozenberger B.A., Eppler C.M., Uhl G.R.
    FEBS Lett. 348:75-79(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  7. "Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned mu, delta, and kappa opioid receptors."
    Wick M.J., Minnerath S.R., Lin X., Elde R.P., Law P.Y., Loh H.H.
    Brain Res. Mol. Brain Res. 27:37-44(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  8. "Molecular cloning of the orphanin FQ receptor gene and differential tissue expression of splice variants in rat."
    Curro D., Song I., Anderson M., Yoo J.H., Del Valle J., Owyang C.
    Gene 266:139-145(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  9. "Modulation of Ca2+ channels by opioid receptor-like 1 receptors natively expressed in rat stellate ganglion neurons innervating cardiac muscle."
    Ruiz-Velasco V., Puhl H.L., Fuller B.C., Sumner A.D.
    J. Pharmacol. Exp. Ther. 314:987-994(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Wistar.
  10. "Involvement of ORL1 receptor and ERK kinase in the orphanin FQ-induced nociception in the nucleus accumbens of rats."
    Chen L.Y., Huang J.X., Yu L.C.
    Regul. Pept. 151:43-47(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiOPRX_RAT
AccessioniPrimary (citable) accession number: P35370
Secondary accession number(s): Q791R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.