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P35370 (OPRX_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nociceptin receptor
Alternative name(s):
Kappa-type 3 opioid receptor
Short name=KOR-3
Orphanin FQ receptor
ROR-C
XOR1
Gene names
Name:Oprl1
Synonyms:Oor, Oprl
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin. Ref.9 Ref.10

Subcellular location

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle By similarity. Note: Ligand binding leads to receptor internalization into cytoplasmic vesicles, decreasing the amount of available receptor at the cell surface By similarity. Internalization requires phosphorylation at Ser-360. Can recycle to the cell membrane.

Tissue specificity

Highly expressed in several brain areas, the intestine, liver and spleen. Detected in sympathetic stellate ganglion neurons. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9

Post-translational modification

Phosphorylation at Ser-360 requires ADRBK2 By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Nociceptin receptor
PRO_0000069983

Regions

Topological domain1 – 4545Extracellular By similarity
Transmembrane46 – 7126Helical; Name=1; By similarity
Topological domain72 – 8413Cytoplasmic By similarity
Transmembrane85 – 10622Helical; Name=2; By similarity
Topological domain107 – 12115Extracellular By similarity
Transmembrane122 – 14322Helical; Name=3; By similarity
Topological domain144 – 16219Cytoplasmic By similarity
Transmembrane163 – 18523Helical; Name=4; By similarity
Topological domain186 – 20823Extracellular By similarity
Transmembrane209 – 23325Helical; Name=5; By similarity
Topological domain234 – 26128Cytoplasmic By similarity
Transmembrane262 – 28221Helical; Name=6; By similarity
Topological domain283 – 29715Extracellular By similarity
Transmembrane298 – 31922Helical; Name=7; By similarity
Topological domain320 – 36748Cytoplasmic By similarity

Sites

Site1071Important for G protein-mediated signaling By similarity
Site1271Important for G protein-mediated signaling By similarity

Amino acid modifications

Lipidation3311S-palmitoyl cysteine Potential
Glycosylation211N-linked (GlcNAc...) Potential
Glycosylation261N-linked (GlcNAc...) Potential
Glycosylation361N-linked (GlcNAc...) Potential
Disulfide bond120 ↔ 197 By similarity

Experimental info

Sequence conflict1051G → R in AAA16201. Ref.2
Sequence conflict2261L → V in AAA16201. Ref.2
Sequence conflict2461S → P in AAA16201. Ref.2
Sequence conflict3481S → T Ref.3

Sequences

Sequence LengthMass (Da)Tools
P35370 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: EB2637582747B4AD

FASTA36740,523
        10         20         30         40         50         60 
MESLFPAPYW EVLYGSHFQG NLSLLNETVP HHLLLNASHS AFLPLGLKVT IVGLYLAVCI 

        70         80         90        100        110        120 
GGLLGNCLVM YVILRHTKMK TATNIYIFNL ALADTLVLLT LPFQGTDILL GFWPFGNALC 

       130        140        150        160        170        180 
KTVIAIDYYN MFTSTFTLTA MSVDRYVAIC HPIRALDVRT SSKAQAVNVA IWALASVVGV 

       190        200        210        220        230        240 
PVAIMGSAQV EDEEIECLVE IPAPQDYWGP VFAICIFLFS FIIPVLIISV CYSLMIRRLR 

       250        260        270        280        290        300 
GVRLLSGSRE KDRNLRRITR LVLVVVAVFV GCWTPVQVFV LVQGLGVQPG SETAVAILRF 

       310        320        330        340        350        360 
CTALGYVNSC LNPILYAFLD ENFKACFRKF CCASSLHREM QVSDRVRSIA KDVGLGCKTS 


ETVPRPA 

« Hide

References

[1]"cDNA cloning and regional distribution of a novel member of the opioid receptor family."
Fukuda K., Kato S., Mori K., Nishi M., Takeshima H., Iwabe N., Miyata T., Houtani T., Sugimoto T.
FEBS Lett. 343:42-46(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Brain.
[2]Meng F., Xie G., Alfred M., Thompson R., Hoversten M., Watson S., Akil H.
Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hippocampus.
[3]"Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a mu, delta or kappa opioid receptor type."
Bunzow J.R., Saez C., Mortrud M., Bouvier C., Williams J.T., Low M., Grandy D.K.
FEBS Lett. 347:284-288(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
[4]"Molecular cloning, tissue distribution and chromosomal localization of a novel member of the opioid receptor gene family."
Chen Y., Fan Y., Liu J., Mestek A., Tian M., Kozak C.A., Yu L.
FEBS Lett. 347:279-283(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[5]"Molecular cloning of a novel G protein-coupled receptor related to the opiate receptor family."
Lachowicz J.E., Shen Y., Monsma F.J. Jr., Sibley D.R.
J. Neurochem. 64:34-40(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Brain.
[6]"cDNA cloning of an orphan opiate receptor gene family member and its splice variant."
Wang J.B., Johnson P.S., Imai Y., Persico A.M., Ozenberger B.A., Eppler C.M., Uhl G.R.
FEBS Lett. 348:75-79(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Brain.
[7]"Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned mu, delta, and kappa opioid receptors."
Wick M.J., Minnerath S.R., Lin X., Elde R.P., Law P.Y., Loh H.H.
Brain Res. Mol. Brain Res. 27:37-44(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[8]"Molecular cloning of the orphanin FQ receptor gene and differential tissue expression of splice variants in rat."
Curro D., Song I., Anderson M., Yoo J.H., Del Valle J., Owyang C.
Gene 266:139-145(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[9]"Modulation of Ca2+ channels by opioid receptor-like 1 receptors natively expressed in rat stellate ganglion neurons innervating cardiac muscle."
Ruiz-Velasco V., Puhl H.L., Fuller B.C., Sumner A.D.
J. Pharmacol. Exp. Ther. 314:987-994(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: Wistar.
[10]"Involvement of ORL1 receptor and ERK kinase in the orphanin FQ-induced nociception in the nucleus accumbens of rats."
Chen L.Y., Huang J.X., Yu L.C.
Regul. Pept. 151:43-47(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16438 mRNA. Translation: BAA03908.1.
U05239 mRNA. Translation: AAA16201.1.
U01913 mRNA. Translation: AAA21025.1.
L28144 mRNA. Translation: AAC37661.1.
U07871 mRNA. Translation: AAA69927.1.
L33916 mRNA. Translation: AAA50827.1.
L29419 mRNA. Translation: AAC42041.1.
AF216218 Genomic DNA. Translation: AAF80990.1.
AY152731 mRNA. Translation: AAN77720.1.
PIRI56520.
RefSeqNP_113757.1. NM_031569.3.
XP_006235798.1. XM_006235736.1.
XP_006235799.1. XM_006235737.1.
XP_006235800.1. XM_006235738.1.
UniGeneRn.9762.

3D structure databases

ProteinModelPortalP35370.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP35370.
ChEMBLCHEMBL4503.
GuidetoPHARMACOLOGY320.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP35370.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000045845; ENSRNOP00000047053; ENSRNOG00000016768.
GeneID29256.
KEGGrno:29256.
UCSCRGD:68438. rat.

Organism-specific databases

CTD4987.
RGD68438. Oprl1.

Phylogenomic databases

eggNOGNOG299442.
GeneTreeENSGT00630000089574.
HOGENOMHOG000230486.
HOVERGENHBG106919.
InParanoidP35370.
KOK04216.
OMAVFAICIF.
OrthoDBEOG7BKCVQ.
PhylomeDBP35370.
TreeFamTF315737.

Gene expression databases

GenevestigatorP35370.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608564.
PROP35370.

Entry information

Entry nameOPRX_RAT
AccessionPrimary (citable) accession number: P35370
Secondary accession number(s): Q791R4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries