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Protein

Alpha-1B adrenergic receptor

Gene

ADRA1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.2 Publications

GO - Molecular functioni

  1. alpha1-adrenergic receptor activity Source: ProtInc
  2. protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: ProtInc
  2. adrenergic receptor signaling pathway Source: GOC
  3. adult heart development Source: Ensembl
  4. behavioral response to cocaine Source: Ensembl
  5. blood vessel remodeling Source: Ensembl
  6. cell-cell signaling Source: ProtInc
  7. cell growth Source: Ensembl
  8. cell proliferation Source: ProtInc
  9. glucose homeostasis Source: Ensembl
  10. G-protein coupled receptor signaling pathway Source: ProtInc
  11. intracellular signal transduction Source: ProtInc
  12. locomotory behavior Source: Ensembl
  13. multicellular organismal development Source: ProtInc
  14. negative regulation of glycogen catabolic process Source: Ensembl
  15. organ growth Source: Ensembl
  16. positive regulation of glycogen catabolic process Source: Ensembl
  17. positive regulation of heart rate by epinephrine-norepinephrine Source: Ensembl
  18. positive regulation of MAPK cascade Source: UniProtKB
  19. positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: Ensembl
  20. regulation of cardiac muscle contraction Source: InterPro
  21. regulation of vasoconstriction Source: InterPro
  22. response to amphetamine Source: Ensembl
  23. response to morphine Source: Ensembl
  24. vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure Source: Ensembl
  25. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_16927. Adrenoceptors.
REACT_18283. G alpha (q) signalling events.
REACT_18407. G alpha (12/13) signalling events.
SignaLinkiP35368.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1B adrenergic receptor
Alternative name(s):
Alpha-1B adrenoreceptor
Short name:
Alpha-1B adrenoceptor
Gene namesi
Name:ADRA1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:278. ADRA1B.

Subcellular locationi

Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularBy similarityAdd
BLAST
Transmembranei46 – 7025Helical; Name=1By similarityAdd
BLAST
Topological domaini71 – 8313CytoplasmicBy similarityAdd
BLAST
Transmembranei84 – 10522Helical; Name=2By similarityAdd
BLAST
Topological domaini106 – 11510ExtracellularBy similarity
Transmembranei116 – 14126Helical; Name=3By similarityAdd
BLAST
Topological domaini142 – 16120CytoplasmicBy similarityAdd
BLAST
Transmembranei162 – 18423Helical; Name=4By similarityAdd
BLAST
Topological domaini185 – 20117ExtracellularBy similarityAdd
BLAST
Transmembranei202 – 22423Helical; Name=5By similarityAdd
BLAST
Topological domaini225 – 29571CytoplasmicBy similarityAdd
BLAST
Transmembranei296 – 31924Helical; Name=6By similarityAdd
BLAST
Topological domaini320 – 3267ExtracellularBy similarity
Transmembranei327 – 35125Helical; Name=7By similarityAdd
BLAST
Topological domaini352 – 520169CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
  2. nuclear membrane Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi368 – 38013RGRGR…RRRRR → AGAGAAAAAAAAA: Abolishes targeting to the nuclear membrane of cardiac myocytes. 1 PublicationAdd
BLAST

Organism-specific databases

PharmGKBiPA33.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Alpha-1B adrenergic receptorPRO_0000069069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
Lipidationi365 – 3651S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP35368.
PaxDbiP35368.
PRIDEiP35368.

PTM databases

PhosphoSiteiP35368.

Expressioni

Gene expression databases

BgeeiP35368.
CleanExiHS_ADRA1B.
GenevestigatoriP35368.

Interactioni

Subunit structurei

Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes.1 Publication

Protein-protein interaction databases

BioGridi106657. 2 interactions.
DIPiDIP-33399N.
IntActiP35368. 4 interactions.
MINTiMINT-120889.
STRINGi9606.ENSP00000306662.

Structurei

3D structure databases

ProteinModelPortaliP35368.
SMRiP35368. Positions 48-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi368 – 38013Nuclear localization signalAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi370 – 38011Poly-ArgAdd
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253615.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239244.
HOVERGENiHBG106962.
InParanoidiP35368.
KOiK04136.
OMAiTYRPWTR.
OrthoDBiEOG7H4DTB.
PhylomeDBiP35368.
TreeFamiTF331895.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001115. ADRA1B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF17. PTHR24248:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00556. ADRENRGCA1BR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPDLDTGHN TSAPAHWGEL KNANFTGPNQ TSSNSTLPQL DITRAISVGL
60 70 80 90 100
VLGAFILFAI VGNILVILSV ACNRHLRTPT NYFIVNLAMA DLLLSFTVLP
110 120 130 140 150
FSAALEVLGY WVLGRIFCDI WAAVDVLCCT ASILSLCAIS IDRYIGVRYS
160 170 180 190 200
LQYPTLVTRR KAILALLSVW VLSTVISIGP LLGWKEPAPN DDKECGVTEE
210 220 230 240 250
PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG VMKEMSNSKE
260 270 280 290 300
LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV
310 320 330 340 350
GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC
360 370 380 390 400
SSKEFKRAFV RILGCQCRGR GRRRRRRRRR LGGCAYTYRP WTRGGSLERS
410 420 430 440 450
QSRKDSLDDS GSCLSGSQRT LPSASPSPGY LGRGAPPPVE LCAFPEWKAP
460 470 480 490 500
GALLSLPAPE PPGRRGRHDS GPLFTFKLLT EPESPGTDGG ASNGGCEAAA
510 520
DVANGQPGFK SNMPLAPGQF
Length:520
Mass (Da):56,836
Last modified:October 17, 2006 - v3
Checksum:iF081A23D2F943F7F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti371 – 3711Missing (PubMed:1328250).Curated
Sequence conflicti371 – 3711Missing in AAB59485 (PubMed:7815325).Curated
Sequence conflicti498 – 5014AAAD → PRH (PubMed:1328250).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti51 – 511V → G.
Corresponds to variant rs8192448 [ dbSNP | Ensembl ].
VAR_019510

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99589 Genomic DNA. No translation available.
M99590 Genomic DNA. No translation available.
U03865 mRNA. Translation: AAB60352.1.
L31773 mRNA. Translation: AAB59485.1.
EU332831 Genomic DNA. Translation: ABY87520.1.
BC136568 mRNA. Translation: AAI36569.1.
BC136569 mRNA. Translation: AAI36570.1.
CCDSiCCDS4347.1.
PIRiA45121.
RefSeqiNP_000670.1. NM_000679.3.
UniGeneiHs.368632.

Genome annotation databases

EnsembliENST00000306675; ENSP00000306662; ENSG00000170214.
GeneIDi147.
KEGGihsa:147.
UCSCiuc003lxt.1. human.

Polymorphism databases

DMDMi116241241.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99589 Genomic DNA. No translation available.
M99590 Genomic DNA. No translation available.
U03865 mRNA. Translation: AAB60352.1.
L31773 mRNA. Translation: AAB59485.1.
EU332831 Genomic DNA. Translation: ABY87520.1.
BC136568 mRNA. Translation: AAI36569.1.
BC136569 mRNA. Translation: AAI36570.1.
CCDSiCCDS4347.1.
PIRiA45121.
RefSeqiNP_000670.1. NM_000679.3.
UniGeneiHs.368632.

3D structure databases

ProteinModelPortaliP35368.
SMRiP35368. Positions 48-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106657. 2 interactions.
DIPiDIP-33399N.
IntActiP35368. 4 interactions.
MINTiMINT-120889.
STRINGi9606.ENSP00000306662.

Chemistry

BindingDBiP35368.
ChEMBLiCHEMBL2094251.
DrugBankiDB01614. Acepromazine.
DB00346. Alfuzosin.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00182. Amphetamine.
DB01238. Aripiprazole.
DB01200. Bromocriptine.
DB00248. Cabergoline.
DB01136. Carvedilol.
DB00477. Chlorpromazine.
DB00575. Clonidine.
DB00363. Clozapine.
DB00298. Dapiprazole.
DB01151. Desipramine.
DB01576. Dextroamphetamine.
DB00590. Doxazosin.
DB01142. Doxepin.
DB04855. Dronedarone.
DB06262. Droxidopa.
DB00668. Epinephrine.
DB01049. Ergoloid mesylate.
DB00696. Ergotamine.
DB00800. Fenoldopam.
DB00458. Imipramine.
DB00598. Labetalol.
DB01255. Lisdexamfetamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01365. Mephentermine.
DB01403. Methotrimeprazine.
DB00723. Methoxamine.
DB06148. Mianserin.
DB00211. Midodrine.
DB00370. Mirtazapine.
DB00745. Modafinil.
DB01149. Nefazodone.
DB00622. Nicardipine.
DB00368. Norepinephrine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00935. Oxymetazoline.
DB01267. Paliperidone.
DB01186. Pergolide.
DB01579. Phendimetrazine.
DB00925. Phenoxybenzamine.
DB00388. Phenylephrine.
DB00457. Prazosin.
DB00420. Promazine.
DB01608. Propericiazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00734. Risperidone.
DB06144. Sertindole.
DB06207. Silodosin.
DB00706. Tamsulosin.
DB01162. Terazosin.
DB01622. Thioproperazine.
DB00679. Thioridazine.
DB00726. Trimipramine.
DB06694. Xylometazoline.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi23.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP35368.

Polymorphism databases

DMDMi116241241.

Proteomic databases

MaxQBiP35368.
PaxDbiP35368.
PRIDEiP35368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306675; ENSP00000306662; ENSG00000170214.
GeneIDi147.
KEGGihsa:147.
UCSCiuc003lxt.1. human.

Organism-specific databases

CTDi147.
GeneCardsiGC05P159343.
HGNCiHGNC:278. ADRA1B.
MIMi104220. gene.
neXtProtiNX_P35368.
PharmGKBiPA33.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG253615.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239244.
HOVERGENiHBG106962.
InParanoidiP35368.
KOiK04136.
OMAiTYRPWTR.
OrthoDBiEOG7H4DTB.
PhylomeDBiP35368.
TreeFamiTF331895.

Enzyme and pathway databases

ReactomeiREACT_16927. Adrenoceptors.
REACT_18283. G alpha (q) signalling events.
REACT_18407. G alpha (12/13) signalling events.
SignaLinkiP35368.

Miscellaneous databases

GeneWikiiAlpha-1B_adrenergic_receptor.
GenomeRNAii147.
NextBioi581.
PROiP35368.
SOURCEiSearch...

Gene expression databases

BgeeiP35368.
CleanExiHS_ADRA1B.
GenevestigatoriP35368.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001115. ADRA1B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF17. PTHR24248:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00556. ADRENRGCA1BR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization and expression of the human alpha 1B-adrenergic receptor."
    Ramarao C.S., Denker J.M., Perez D.M., Gaivin R.J., Riek R.P., Graham R.M.
    J. Biol. Chem. 267:21936-21945(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The alpha 1-adrenergic receptor that mediates smooth muscle contraction in human prostate has the pharmacological properties of the cloned human alpha 1c subtype."
    Forray C., Bard J.A., Wetzel J.M., Chiu G., Shapiro E., Tang R., Lepor H., Hartig P.R., Weinshank R.L., Branchek T.A., Gluchowski C.
    Mol. Pharmacol. 45:703-708(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Cloning and pharmacological characterization of human alpha-1 adrenergic receptors: sequence corrections and direct comparison with other species homologues."
    Schwinn D.A., Johnston G.I., Page S.O., Mosley M.J., Wilson K.H., Worman N.P., Campbell S., Fidock M.D., Furness L.M., Parry-Smith D.J., Peter B., Bailey D.S.
    J. Pharmacol. Exp. Ther. 272:134-142(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. NIEHS SNPs program
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Nuclear alpha1-adrenergic receptors signal activated ERK localization to caveolae in adult cardiac myocytes."
    Wright C.D., Chen Q., Baye N.L., Huang Y., Healy C.L., Kasinathan S., O'Connell T.D.
    Circ. Res. 103:992-1000(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  7. "Nuclear localization drives alpha1-adrenergic receptor oligomerization and signaling in cardiac myocytes."
    Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D.
    Cell. Signal. 24:794-802(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, MUTAGENESIS OF 368-ARG--ARG-380.

Entry informationi

Entry nameiADA1B_HUMAN
AccessioniPrimary (citable) accession number: P35368
Secondary accession number(s): B0LPE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: February 4, 2015
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.