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P35368 (ADA1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1B adrenergic receptor
Alternative name(s):
Alpha-1B adrenoreceptor
Short name=Alpha-1B adrenoceptor
Gene names
Name:ADRA1B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G11 proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. Ref.6 Ref.7

Subunit structure

Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes. Ref.7

Subcellular location

Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. Ref.6 Ref.7

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Nucleus
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 2154750. Source: ProtInc

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Traceable author statement PubMed 10820200. Source: ProtInc

adrenergic receptor signaling pathway

Traceable author statement Ref.1. Source: GOC

adult heart development

Inferred from electronic annotation. Source: Ensembl

behavioral response to cocaine

Inferred from electronic annotation. Source: Ensembl

blood vessel remodeling

Inferred from electronic annotation. Source: Ensembl

cell growth

Inferred from electronic annotation. Source: Ensembl

cell proliferation

Traceable author statement PubMed 10820200. Source: ProtInc

cell-cell signaling

Traceable author statement Ref.1. Source: ProtInc

glucose homeostasis

Inferred from electronic annotation. Source: Ensembl

intracellular signal transduction

Traceable author statement PubMed 10820200. Source: ProtInc

locomotory behavior

Inferred from electronic annotation. Source: Ensembl

multicellular organismal development

Traceable author statement PubMed 10820200. Source: ProtInc

negative regulation of glycogen catabolic process

Inferred from electronic annotation. Source: Ensembl

organ growth

Inferred from electronic annotation. Source: Ensembl

positive regulation of MAPK cascade

Inferred from direct assay Ref.7. Source: UniProtKB

positive regulation of glycogen catabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of heart rate by epinephrine-norepinephrine

Inferred from electronic annotation. Source: Ensembl

positive regulation of the force of heart contraction by epinephrine-norepinephrine

Inferred from electronic annotation. Source: Ensembl

regulation of cardiac muscle contraction

Inferred from electronic annotation. Source: InterPro

regulation of vasoconstriction

Inferred from electronic annotation. Source: InterPro

response to amphetamine

Inferred from electronic annotation. Source: Ensembl

response to morphine

Inferred from electronic annotation. Source: Ensembl

vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

visual learning

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentintegral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

nuclear membrane

Inferred from direct assay Ref.7. Source: UniProtKB

nucleus

Inferred from direct assay Ref.7. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionalpha1-adrenergic receptor activity

Traceable author statement Ref.1. Source: ProtInc

protein heterodimerization activity

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Alpha-1B adrenergic receptor
PRO_0000069069

Regions

Topological domain1 – 4545Extracellular By similarity
Transmembrane46 – 7025Helical; Name=1; By similarity
Topological domain71 – 8313Cytoplasmic By similarity
Transmembrane84 – 10522Helical; Name=2; By similarity
Topological domain106 – 11510Extracellular By similarity
Transmembrane116 – 14126Helical; Name=3; By similarity
Topological domain142 – 16120Cytoplasmic By similarity
Transmembrane162 – 18423Helical; Name=4; By similarity
Topological domain185 – 20117Extracellular By similarity
Transmembrane202 – 22423Helical; Name=5; By similarity
Topological domain225 – 29571Cytoplasmic By similarity
Transmembrane296 – 31924Helical; Name=6; By similarity
Topological domain320 – 3267Extracellular By similarity
Transmembrane327 – 35125Helical; Name=7; By similarity
Topological domain352 – 520169Cytoplasmic By similarity
Region368 – 38013Nuclear localization signal
Compositional bias370 – 38011Poly-Arg

Amino acid modifications

Lipidation3651S-palmitoyl cysteine Potential
Glycosylation101N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...) Potential
Disulfide bond118 ↔ 195 By similarity

Natural variations

Natural variant511V → G.
Corresponds to variant rs8192448 [ dbSNP | Ensembl ].
VAR_019510

Experimental info

Mutagenesis368 – 38013RGRGR…RRRRR → AGAGAAAAAAAAA: Abolishes targeting to the nuclear membrane of cardiac myocytes. Ref.7
Sequence conflict3711Missing Ref.1
Sequence conflict3711Missing in AAB59485. Ref.3
Sequence conflict498 – 5014AAAD → PRH Ref.1

Sequences

Sequence LengthMass (Da)Tools
P35368 [UniParc].

Last modified October 17, 2006. Version 3.
Checksum: F081A23D2F943F7F

FASTA52056,836
        10         20         30         40         50         60 
MNPDLDTGHN TSAPAHWGEL KNANFTGPNQ TSSNSTLPQL DITRAISVGL VLGAFILFAI 

        70         80         90        100        110        120 
VGNILVILSV ACNRHLRTPT NYFIVNLAMA DLLLSFTVLP FSAALEVLGY WVLGRIFCDI 

       130        140        150        160        170        180 
WAAVDVLCCT ASILSLCAIS IDRYIGVRYS LQYPTLVTRR KAILALLSVW VLSTVISIGP 

       190        200        210        220        230        240 
LLGWKEPAPN DDKECGVTEE PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG 

       250        260        270        280        290        300 
VMKEMSNSKE LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV 

       310        320        330        340        350        360 
GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC SSKEFKRAFV 

       370        380        390        400        410        420 
RILGCQCRGR GRRRRRRRRR LGGCAYTYRP WTRGGSLERS QSRKDSLDDS GSCLSGSQRT 

       430        440        450        460        470        480 
LPSASPSPGY LGRGAPPPVE LCAFPEWKAP GALLSLPAPE PPGRRGRHDS GPLFTFKLLT 

       490        500        510        520 
EPESPGTDGG ASNGGCEAAA DVANGQPGFK SNMPLAPGQF 

« Hide

References

« Hide 'large scale' references
[1]"Genomic organization and expression of the human alpha 1B-adrenergic receptor."
Ramarao C.S., Denker J.M., Perez D.M., Gaivin R.J., Riek R.P., Graham R.M.
J. Biol. Chem. 267:21936-21945(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The alpha 1-adrenergic receptor that mediates smooth muscle contraction in human prostate has the pharmacological properties of the cloned human alpha 1c subtype."
Forray C., Bard J.A., Wetzel J.M., Chiu G., Shapiro E., Tang R., Lepor H., Hartig P.R., Weinshank R.L., Branchek T.A., Gluchowski C.
Mol. Pharmacol. 45:703-708(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Cloning and pharmacological characterization of human alpha-1 adrenergic receptors: sequence corrections and direct comparison with other species homologues."
Schwinn D.A., Johnston G.I., Page S.O., Mosley M.J., Wilson K.H., Worman N.P., Campbell S., Fidock M.D., Furness L.M., Parry-Smith D.J., Peter B., Bailey D.S.
J. Pharmacol. Exp. Ther. 272:134-142(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]NIEHS SNPs program
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Nuclear alpha1-adrenergic receptors signal activated ERK localization to caveolae in adult cardiac myocytes."
Wright C.D., Chen Q., Baye N.L., Huang Y., Healy C.L., Kasinathan S., O'Connell T.D.
Circ. Res. 103:992-1000(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION.
[7]"Nuclear localization drives alpha1-adrenergic receptor oligomerization and signaling in cardiac myocytes."
Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D.
Cell. Signal. 24:794-802(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, MUTAGENESIS OF 368-ARG--ARG-380.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M99589 Genomic DNA. No translation available.
M99590 Genomic DNA. No translation available.
U03865 mRNA. Translation: AAB60352.1.
L31773 mRNA. Translation: AAB59485.1.
EU332831 Genomic DNA. Translation: ABY87520.1.
BC136568 mRNA. Translation: AAI36569.1.
BC136569 mRNA. Translation: AAI36570.1.
PIRA45121.
RefSeqNP_000670.1. NM_000679.3.
UniGeneHs.368632.

3D structure databases

ProteinModelPortalP35368.
SMRP35368. Positions 48-364.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106657. 2 interactions.
DIPDIP-33399N.
IntActP35368. 4 interactions.
MINTMINT-120889.
STRING9606.ENSP00000306662.

Chemistry

BindingDBP35368.
ChEMBLCHEMBL2094251.
DrugBankDB00346. Alfuzosin.
DB00217. Bethanidine.
DB00298. Dapiprazole.
DB04840. Debrisoquin.
DB01576. Dextroamphetamine.
DB00590. Doxazosin.
DB00226. Guanadrel Sulfate.
DB01170. Guanethidine.
DB01018. Guanfacine.
DB00598. Labetalol.
DB01255. Lisdexamfetamine.
DB01577. Methamphetamine.
DB01403. Methotrimeprazine.
DB00723. Methoxamine.
DB00211. Midodrine.
DB00745. Modafinil.
DB01149. Nefazodone.
DB00368. Norepinephrine.
DB00334. Olanzapine.
DB01579. Phendimetrazine.
DB00388. Phenylephrine.
DB00457. Prazosin.
DB00420. Promazine.
DB01608. Propericiazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00734. Risperidone.
DB06144. Sertindole.
DB00706. Tamsulosin.
DB01162. Terazosin.
DB00656. Trazodone.
GuidetoPHARMACOLOGY23.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP35368.

Polymorphism databases

DMDM116241241.

Proteomic databases

PaxDbP35368.
PRIDEP35368.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306675; ENSP00000306662; ENSG00000170214.
GeneID147.
KEGGhsa:147.
UCSCuc003lxt.1. human.

Organism-specific databases

CTD147.
GeneCardsGC05P159276.
HGNCHGNC:278. ADRA1B.
MIM104220. gene.
neXtProtNX_P35368.
PharmGKBPA33.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG253615.
HOGENOMHOG000239244.
HOVERGENHBG106962.
InParanoidP35368.
KOK04136.
OMATYRPWTR.
OrthoDBEOG7H4DTB.
PhylomeDBP35368.
TreeFamTF331895.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
SignaLinkP35368.

Gene expression databases

BgeeP35368.
CleanExHS_ADRA1B.
GenevestigatorP35368.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002233. ADR_fam.
IPR001115. ADRA1B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24248. PTHR24248. 1 hit.
PTHR24248:SF17. PTHR24248:SF17. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01103. ADRENERGICR.
PR00556. ADRENRGCA1BR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiAlpha-1B_adrenergic_receptor.
GenomeRNAi147.
NextBio581.
PROP35368.
SOURCESearch...

Entry information

Entry nameADA1B_HUMAN
AccessionPrimary (citable) accession number: P35368
Secondary accession number(s): B0LPE1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries