P35368 (ADA1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1B adrenergic receptor

Alternative name(s):
Alpha-1B adrenoreceptor
Short name=Alpha-1B adrenoceptor

Gene names

Name:

ADRA1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G11 proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. Ref.6 Ref.7
Subunit structure	Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes. Ref.7
Subcellular location	Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. Ref.6 Ref.7
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Nucleus
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-modulating G-protein coupled receptor signaling pathway Traceable author statement PubMed 10820200. Source: ProtInc adult heart development Inferred from electronic annotation. Source: Compara behavioral response to cocaine Inferred from electronic annotation. Source: Compara blood vessel remodeling Inferred from electronic annotation. Source: Compara cell growth Inferred from electronic annotation. Source: Compara cell proliferation Traceable author statement PubMed 10820200. Source: ProtInc cell-cell signaling Traceable author statement Ref.1. Source: ProtInc glucose homeostasis Inferred from electronic annotation. Source: Compara intracellular protein kinase cascade Traceable author statement PubMed 10820200. Source: ProtInc locomotory behavior Inferred from electronic annotation. Source: Compara multicellular organismal development Traceable author statement PubMed 10820200. Source: ProtInc negative regulation of glycogen catabolic process Inferred from electronic annotation. Source: Compara organ growth Inferred from electronic annotation. Source: Compara positive regulation of MAPK cascade Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of glycogen catabolic process Inferred from electronic annotation. Source: Compara positive regulation of heart rate by epinephrine-norepinephrine Inferred from electronic annotation. Source: Compara positive regulation of the force of heart contraction by epinephrine-norepinephrine Inferred from electronic annotation. Source: Compara response to amphetamine Inferred from electronic annotation. Source: Compara response to morphine Inferred from electronic annotation. Source: Compara vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara visual learning Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc nuclear membrane Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	alpha1-adrenergic receptor activity Traceable author statement Ref.1. Source: ProtInc protein heterodimerization activity Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Alpha-1B adrenergic receptor		PRO_0000069069
Regions
	Topological domain	1 – 45	45	Extracellular By similarity
	Transmembrane	46 – 70	25	Helical; Name=1; By similarity
	Topological domain	71 – 83	13	Cytoplasmic By similarity
	Transmembrane	84 – 105	22	Helical; Name=2; By similarity
	Topological domain	106 – 115	10	Extracellular By similarity
	Transmembrane	116 – 141	26	Helical; Name=3; By similarity
	Topological domain	142 – 161	20	Cytoplasmic By similarity
	Transmembrane	162 – 184	23	Helical; Name=4; By similarity
	Topological domain	185 – 201	17	Extracellular By similarity
	Transmembrane	202 – 224	23	Helical; Name=5; By similarity
	Topological domain	225 – 295	71	Cytoplasmic By similarity
	Transmembrane	296 – 319	24	Helical; Name=6; By similarity
	Topological domain	320 – 326	7	Extracellular By similarity
	Transmembrane	327 – 351	25	Helical; Name=7; By similarity
	Topological domain	352 – 520	169	Cytoplasmic By similarity
	Region	368 – 380	13	Nuclear localization signal
	Compositional bias	370 – 380	11	Poly-Arg
Amino acid modifications
	Modified residue	264	1	Phosphothreonine By similarity
	Modified residue	267	1	Phosphoserine By similarity
	Lipidation	365	1	S-palmitoyl cysteine Potential
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 195		By similarity
Natural variations
	Natural variant	51	1	V → G. Corresponds to variant rs8192448 [ dbSNP | Ensembl ].		VAR_019510
Experimental info
	Mutagenesis	368 – 380	13	RGRGR…RRRRR → AGAGAAAAAAAAA: Abolishes targeting to the nuclear membrane of cardiac myocytes. Ref.7
	Sequence conflict	371	1	Missing Ref.1
	Sequence conflict	371	1	Missing in AAB59485. Ref.3
	Sequence conflict	498 – 501	4	AAAD → PRH Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35368 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: F081A23D2F943F7F	FASTA	520	56,836
	10 20 30 40 50 60 MNPDLDTGHN TSAPAHWGEL KNANFTGPNQ TSSNSTLPQL DITRAISVGL VLGAFILFAI 70 80 90 100 110 120 VGNILVILSV ACNRHLRTPT NYFIVNLAMA DLLLSFTVLP FSAALEVLGY WVLGRIFCDI 130 140 150 160 170 180 WAAVDVLCCT ASILSLCAIS IDRYIGVRYS LQYPTLVTRR KAILALLSVW VLSTVISIGP 190 200 210 220 230 240 LLGWKEPAPN DDKECGVTEE PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG 250 260 270 280 290 300 VMKEMSNSKE LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV 310 320 330 340 350 360 GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC SSKEFKRAFV 370 380 390 400 410 420 RILGCQCRGR GRRRRRRRRR LGGCAYTYRP WTRGGSLERS QSRKDSLDDS GSCLSGSQRT 430 440 450 460 470 480 LPSASPSPGY LGRGAPPPVE LCAFPEWKAP GALLSLPAPE PPGRRGRHDS GPLFTFKLLT 490 500 510 520 EPESPGTDGG ASNGGCEAAA DVANGQPGFK SNMPLAPGQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization and expression of the human alpha 1B-adrenergic receptor." Ramarao C.S., Denker J.M., Perez D.M., Gaivin R.J., Riek R.P., Graham R.M. J. Biol. Chem. 267:21936-21945(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The alpha 1-adrenergic receptor that mediates smooth muscle contraction in human prostate has the pharmacological properties of the cloned human alpha 1c subtype." Forray C., Bard J.A., Wetzel J.M., Chiu G., Shapiro E., Tang R., Lepor H., Hartig P.R., Weinshank R.L., Branchek T.A., Gluchowski C. Mol. Pharmacol. 45:703-708(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Cloning and pharmacological characterization of human alpha-1 adrenergic receptors: sequence corrections and direct comparison with other species homologues." Schwinn D.A., Johnston G.I., Page S.O., Mosley M.J., Wilson K.H., Worman N.P., Campbell S., Fidock M.D., Furness L.M., Parry-Smith D.J., Peter B., Bailey D.S. J. Pharmacol. Exp. Ther. 272:134-142(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	NIEHS SNPs program Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Nuclear alpha1-adrenergic receptors signal activated ERK localization to caveolae in adult cardiac myocytes." Wright C.D., Chen Q., Baye N.L., Huang Y., Healy C.L., Kasinathan S., O'Connell T.D. Circ. Res. 103:992-1000(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION.
[7]	"Nuclear localization drives alpha1-adrenergic receptor oligomerization and signaling in cardiac myocytes." Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D. Cell. Signal. 24:794-802(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, MUTAGENESIS OF 368-ARG--ARG-380.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M99589 Genomic DNA. No translation available. M99590 Genomic DNA. No translation available. U03865 mRNA. Translation: AAB60352.1. L31773 mRNA. Translation: AAB59485.1. EU332831 Genomic DNA. Translation: ABY87520.1. BC136568 mRNA. Translation: AAI36569.1. BC136569 mRNA. Translation: AAI36570.1.
IPI	IPI00218912.
PIR	A45121.
RefSeq	NP_000670.1. NM_000679.3.
UniGene	Hs.368632.
3D structure databases
ProteinModelPortal	P35368.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-33399N.
IntAct	P35368. 3 interactions.
MINT	MINT-120889.
STRING	9606.ENSP00000306662.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35368.
Polymorphism databases
DMDM	116241241.
Proteomic databases
PaxDb	P35368.
PRIDE	P35368.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306675; ENSP00000306662; ENSG00000170214.
GeneID	147.
KEGG	hsa:147.
UCSC	uc003lxt.1. human.
Organism-specific databases
CTD	147.
GeneCards	GC05P159276.
HGNC	HGNC:278. ADRA1B.
MIM	104220. gene.
neXtProt	NX_P35368.
PharmGKB	PA33.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG253615.
HOGENOM	HOG000239244.
HOVERGEN	HBG106962.
InParanoid	P35368.
KO	K04136.
OMA	TYRPWTR.
OrthoDB	EOG480HWT.
PhylomeDB	P35368.
Enzyme and pathway databases
Pathway_Interaction_DB	lysophospholipid_pathway. LPA receptor mediated events.
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P35368.
CleanEx	HS_ADRA1B.
Genevestigator	P35368.
GermOnline	ENSG00000170214. Homo sapiens.
Family and domain databases
InterPro	IPR001115. Adrene_rcpt_A1B. IPR002233. Adrnrgc_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00556. ADRENRGCA1BR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P35368.
ChEMBL	CHEMBL232.
DrugBank	DB00346. Alfuzosin. DB00217. Bethanidine. DB00298. Dapiprazole. DB04840. Debrisoquin. DB01576. Dextroamphetamine. DB00590. Doxazosin. DB00226. Guanadrel Sulfate. DB01170. Guanethidine. DB01018. Guanfacine. DB00598. Labetalol. DB01255. Lisdexamfetamine. DB01577. Methamphetamine. DB01403. Methotrimeprazine. DB00723. Methoxamine. DB00211. Midodrine. DB00745. Modafinil. DB01149. Nefazodone. DB00368. Norepinephrine. DB00334. Olanzapine. DB01579. Phendimetrazine. DB00388. Phenylephrine. DB00457. Prazosin. DB00420. Promazine. DB01608. Propericiazine. DB00777. Propiomazine. DB01224. Quetiapine. DB00734. Risperidone. DB06144. Sertindole. DB00706. Tamsulosin. DB01162. Terazosin. DB00656. Trazodone.
GenomeRNAi	147.
NextBio	581.
SOURCE	Search...

Entry information

Entry name

ADA1B_HUMAN

Accession

Primary (citable) accession number: P35368
Secondary accession number(s): B0LPE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.