P35368 (ADA1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 116.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-1B adrenergic receptor Alternative name(s): Alpha-1B adrenoreceptor Short name=Alpha-1B adrenoceptor | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Reference proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 520 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G11 proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. Ref.6 Ref.7 |
| Subunit structure | Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes. Ref.7 |
| Subcellular location | Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. Ref.6 Ref.7 |
| Sequence similarities | Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 520 | 520 | Alpha-1B adrenergic receptor | PRO_0000069069 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 45 | 45 | Extracellular By similarity | ||||||||
| Transmembrane | 46 – 70 | 25 | Helical; Name=1; By similarity | ||||||||
| Topological domain | 71 – 83 | 13 | Cytoplasmic By similarity | ||||||||
| Transmembrane | 84 – 105 | 22 | Helical; Name=2; By similarity | ||||||||
| Topological domain | 106 – 115 | 10 | Extracellular By similarity | ||||||||
| Transmembrane | 116 – 141 | 26 | Helical; Name=3; By similarity | ||||||||
| Topological domain | 142 – 161 | 20 | Cytoplasmic By similarity | ||||||||
| Transmembrane | 162 – 184 | 23 | Helical; Name=4; By similarity | ||||||||
| Topological domain | 185 – 201 | 17 | Extracellular By similarity | ||||||||
| Transmembrane | 202 – 224 | 23 | Helical; Name=5; By similarity | ||||||||
| Topological domain | 225 – 295 | 71 | Cytoplasmic By similarity | ||||||||
| Transmembrane | 296 – 319 | 24 | Helical; Name=6; By similarity | ||||||||
| Topological domain | 320 – 326 | 7 | Extracellular By similarity | ||||||||
| Transmembrane | 327 – 351 | 25 | Helical; Name=7; By similarity | ||||||||
| Topological domain | 352 – 520 | 169 | Cytoplasmic By similarity | ||||||||
| Region | 368 – 380 | 13 | Nuclear localization signal | ||||||||
| Compositional bias | 370 – 380 | 11 | Poly-Arg | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 264 | 1 | Phosphothreonine By similarity | ||||||||
| Modified residue | 267 | 1 | Phosphoserine By similarity | ||||||||
| Lipidation | 365 | 1 | S-palmitoyl cysteine Potential | ||||||||
| Glycosylation | 10 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 24 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 29 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 34 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 118 ↔ 195 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 51 | 1 | V → G. Corresponds to variant rs8192448 [ dbSNP | Ensembl ]. | VAR_019510 | |||||||
Experimental info | |||||||||||
| Mutagenesis | 368 – 380 | 13 | RGRGR…RRRRR → AGAGAAAAAAAAA: Abolishes targeting to the nuclear membrane of cardiac myocytes. Ref.7 | ||||||||
| Sequence conflict | 371 | 1 | Missing Ref.1 | ||||||||
| Sequence conflict | 371 | 1 | Missing in AAB59485. Ref.3 | ||||||||
| Sequence conflict | 498 – 501 | 4 | AAAD → PRH Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Genomic organization and expression of the human alpha 1B-adrenergic receptor." Ramarao C.S., Denker J.M., Perez D.M., Gaivin R.J., Riek R.P., Graham R.M. J. Biol. Chem. 267:21936-21945(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The alpha 1-adrenergic receptor that mediates smooth muscle contraction in human prostate has the pharmacological properties of the cloned human alpha 1c subtype." Forray C., Bard J.A., Wetzel J.M., Chiu G., Shapiro E., Tang R., Lepor H., Hartig P.R., Weinshank R.L., Branchek T.A., Gluchowski C. Mol. Pharmacol. 45:703-708(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [3] | "Cloning and pharmacological characterization of human alpha-1 adrenergic receptors: sequence corrections and direct comparison with other species homologues." Schwinn D.A., Johnston G.I., Page S.O., Mosley M.J., Wilson K.H., Worman N.P., Campbell S., Fidock M.D., Furness L.M., Parry-Smith D.J., Peter B., Bailey D.S. J. Pharmacol. Exp. Ther. 272:134-142(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | NIEHS SNPs program Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [6] | "Nuclear alpha1-adrenergic receptors signal activated ERK localization to caveolae in adult cardiac myocytes." Wright C.D., Chen Q., Baye N.L., Huang Y., Healy C.L., Kasinathan S., O'Connell T.D. Circ. Res. 103:992-1000(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION. |
| [7] | "Nuclear localization drives alpha1-adrenergic receptor oligomerization and signaling in cardiac myocytes." Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D. Cell. Signal. 24:794-802(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, MUTAGENESIS OF 368-ARG--ARG-380. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M99589 Genomic DNA. No translation available. M99590 Genomic DNA. No translation available. U03865 mRNA. Translation: AAB60352.1. L31773 mRNA. Translation: AAB59485.1. EU332831 Genomic DNA. Translation: ABY87520.1. BC136568 mRNA. Translation: AAI36569.1. BC136569 mRNA. Translation: AAI36570.1. |
| IPI | IPI00218912. |
| PIR | A45121. |
| RefSeq | NP_000670.1. NM_000679.3. |
| UniGene | Hs.368632. |
3D structure databases | |
| ProteinModelPortal | P35368. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-33399N. |
| IntAct | P35368. 3 interactions. |
| MINT | MINT-120889. |
| STRING | 9606.ENSP00000306662. |
Protein family/group databases | |
| GPCRDB | Search... |
PTM databases | |
| PhosphoSite | P35368. |
Polymorphism databases | |
| DMDM | 116241241. |
Proteomic databases | |
| PaxDb | P35368. |
| PRIDE | P35368. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000306675; ENSP00000306662; ENSG00000170214. |
| GeneID | 147. |
| KEGG | hsa:147. |
| UCSC | uc003lxt.1. human. |
Organism-specific databases | |
| CTD | 147. |
| GeneCards | GC05P159276. |
| HGNC | HGNC:278. ADRA1B. |
| MIM | 104220. gene. |
| neXtProt | NX_P35368. |
| PharmGKB | PA33. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | NOG253615. |
| HOGENOM | HOG000239244. |
| HOVERGEN | HBG106962. |
| InParanoid | P35368. |
| KO | K04136. |
| OMA | TYRPWTR. |
| OrthoDB | EOG480HWT. |
| PhylomeDB | P35368. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | lysophospholipid_pathway. LPA receptor mediated events. |
| Reactome | REACT_111102. Signal Transduction. |
Gene expression databases | |
| Bgee | P35368. |
| CleanEx | HS_ADRA1B. |
| Genevestigator | P35368. |
| GermOnline | ENSG00000170214. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001115. Adrene_rcpt_A1B. IPR002233. Adrnrgc_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view] |
| Pfam | PF00001. 7tm_1. 1 hit. [Graphical view] |
| PRINTS | PR01103. ADRENERGICR. PR00556. ADRENRGCA1BR. PR00237. GPCRRHODOPSN. |
| PROSITE | PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| BindingDB | P35368. |
| ChEMBL | CHEMBL232. |
| DrugBank | DB00346. Alfuzosin. DB00217. Bethanidine. DB00298. Dapiprazole. DB04840. Debrisoquin. DB01576. Dextroamphetamine. DB00590. Doxazosin. DB00226. Guanadrel Sulfate. DB01170. Guanethidine. DB01018. Guanfacine. DB00598. Labetalol. DB01255. Lisdexamfetamine. DB01577. Methamphetamine. DB01403. Methotrimeprazine. DB00723. Methoxamine. DB00211. Midodrine. DB00745. Modafinil. DB01149. Nefazodone. DB00368. Norepinephrine. DB00334. Olanzapine. DB01579. Phendimetrazine. DB00388. Phenylephrine. DB00457. Prazosin. DB00420. Promazine. DB01608. Propericiazine. DB00777. Propiomazine. DB01224. Quetiapine. DB00734. Risperidone. DB06144. Sertindole. DB00706. Tamsulosin. DB01162. Terazosin. DB00656. Trazodone. |
| GenomeRNAi | 147. |
| NextBio | 581. |
| SOURCE | Search... |
Entry information
| Entry name | ADA1B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P35368 Secondary accession number(s): B0LPE1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| 7-transmembrane G-linked receptors List of 7-transmembrane G-linked receptor entries |
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
