Reviewed, UniProtKB/Swiss-Prot P35367 (HRH1_HUMAN)

Names and origin

Protein names

Recommended name:
Histamine H1 receptor
Short name=HH1R
Short name=H1R

Gene names

Name:

HRH1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger Ref.1 Traceable author statement. Source: ProtInc inflammatory response Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.3 Traceable author statement. Source: ProtInc
Molecular function	histamine receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Histamine H1 receptor		PRO_0000069676
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 49	20	1 Potential
	Topological domain	50 – 63	14	Cytoplasmic Potential
	Transmembrane	64 – 83	20	2 Potential
	Topological domain	84 – 101	18	Extracellular Potential
	Transmembrane	102 – 123	22	3 Potential
	Topological domain	124 – 145	22	Cytoplasmic Potential
	Transmembrane	146 – 165	20	4 Potential
	Topological domain	166 – 189	24	Extracellular Potential
	Transmembrane	190 – 210	21	5 Potential
	Topological domain	211 – 418	208	Cytoplasmic Potential
	Transmembrane	419 – 438	20	6 Potential
	Topological domain	439 – 450	12	Extracellular Potential
	Transmembrane	451 – 470	20	7 Potential
	Topological domain	471 – 487	17	Cytoplasmic Potential
Amino acid modifications
	Modified residue	255	1	Phosphoserine Ref.10
	Modified residue	275	1	Phosphoserine Ref.10 Ref.11
	Modified residue	279	1	Phosphothreonine Ref.11
	Lipidation	445	1	S-palmitoyl cysteine Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Disulfide bond	100 ↔ 180		By similarity
Natural variations
	Natural variant	19	1	K → N: dbSNP rs2067466.		VAR_049410
	Natural variant	270	1	G → E: dbSNP rs7651620.		VAR_033476
	Natural variant	385	1	D → E in a colorectal cancer sample; somatic mutation. Ref.12		VAR_035761
Experimental info
	Sequence conflict	308	1	V → E in AAH60802. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P35367-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: E5DB418A4C17A985	FASTA	487	55,784
	10 20 30 40 50 60 MSLPNSSCLL EDKMCEGNKT TMASPQLMPL VVVLSTICLV TVGLNLLVLY AVRSERKLHT 70 80 90 100 110 120 VGNLYIVSLS VADLIVGAVV MPMNILYLLM SKWSLGRPLC LFWLSMDYVA STASIFSVFI 130 140 150 160 170 180 LCIDRYRSVQ QPLRYLKYRT KTRASATILG AWFLSFLWVI PILGWNHFMQ QTSVRREDKC 190 200 210 220 230 240 ETDFYDVTWF KVMTAIINFY LPTLLMLWFY AKIYKAVRQH CQHRELINRS LPSFSEIKLR 250 260 270 280 290 300 PENPKGDAKK PGKESPWEVL KRKPKDAGGG SVLKSPSQTP KEMKSPVVFS QEDDREVDKL 310 320 330 340 350 360 YCFPLDIVHM QAAAEGSSRD YVAVNRSHGQ LKTDEQGLNT HGASEISEDQ MLGDSQSFSR 370 380 390 400 410 420 TDSDTTTETA PGKGKLRSGS NTGLDYIKFT WKRLRSHSRQ YVSGLHMNRE RKAAKQLGFI 430 440 450 460 470 480 MAAFILCWIP YFIFFMVIAF CKNCCNEHLH MFTIWLGYIN STLNPLIYPL CNENFKKTFK RILHIRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic cloning, heterologous expression and pharmacological characterization of a human histamine H1 receptor." de Backer M.D., Gommeren W., Moereels H., Nobels G., van Gompel P., Leysen J.E., Luyten W.H.M.L. Biochem. Biophys. Res. Commun. 197:1601-1608(1993) [PubMed: 8280179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of the human histamine H1 receptor gene." Fukui K., Fujimoto K., Mizuguchi H., Sakamoto K., Horio Y., Takai S., Yamada K., Ito S. Biochem. Biophys. Res. Commun. 201:894-901(1994) [PubMed: 8003029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Stable expression of human H1-histamine-receptor cDNA in Chinese hamster ovary cells. Pharmacological characterisation of the protein, tissue distribution of messenger RNA and chromosomal localisation of the gene." Moguilevsky N., Varsalona F., Noyer M., Gillard M., Guillaume J.P., Garcia L., Szpirer C., Szpirer J., Bollen A. Eur. J. Biochem. 224:489-495(1994) [PubMed: 7925364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	Rae J.L., Shepard A.R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens epithelium.
[5]	"Human-specific amino acid changes found in 103 protein-coding genes." Kitano T., Liu Y.-H., Ueda S., Saitou N. Mol. Biol. Evol. 21:936-944(2004) [PubMed: 15014171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255 AND SER-275, MASS SPECTROMETRY.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275 AND THR-279, MASS SPECTROMETRY.
[12]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-385.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34897 mRNA. Translation: CAA84380.1. X76786 Genomic DNA. Translation: CAA54182.1. D14436 Genomic DNA. Translation: BAA03319.1. D28481 mRNA. Translation: BAA05840.1. AF026261 mRNA. Translation: AAB95156.1. AB041380 Genomic DNA. Translation: BAA94465.1. AY136743 mRNA. Translation: AAN01269.1. AK289412 mRNA. Translation: BAF82101.1. CH471055 Genomic DNA. Translation: EAW64092.1. BC060802 mRNA. Translation: AAH60802.1.
IPI	IPI00018147.
PIR	JC2495.
RefSeq	NP_000852.1. NP_001091681.1. NP_001091682.1. NP_001091683.1.
UniGene	Hs.1570
3D structure databases
SMR	P35367. Positions 27-484.
ModBase	Search...
Protein-protein interaction databases
STRING	P35367.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35367.
Proteomic databases
PRIDE	P35367.
Genome annotation databases
Ensembl	ENST00000355172; ENSP00000347299; ENSG00000196639; Homo sapiens. [Genome view] ENST00000397056; ENSP00000380247; ENSG00000196639; Homo sapiens. [Genome view] ENST00000431010; ENSP00000397028; ENSG00000196639; Homo sapiens. [Genome view] ENST00000438284; ENSP00000406705; ENSG00000196639; Homo sapiens. [Genome view]
GeneID	3269.
KEGG	hsa:3269.
UCSC	uc003bwb.2. human.
Organism-specific databases
CTD	3269.
GeneCards	GC03P011223.
H-InvDB	HIX0030701.
HGNC	HGNC:5182. HRH1.
MIM	600167. gene.
PharmGKB	PA29456.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13104.
HOGENOM	HBG447339.
HOVERGEN	HBG101103.
InParanoid	P35367.
OMA	LHMFTIW.
OrthoDB	EOG9M94B3.
PhylomeDB	P35367.
Enzyme and pathway databases
Reactome	REACT_14797. Signaling by GPCR.
Gene expression databases
ArrayExpress	P35367.
Bgee	P35367.
CleanEx	HS_HRH1.
Genevestigator	P35367.
GermOnline	ENSG00000196639. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000921. Histamine_H1_recept. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00530. HISTAMINEH1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	P35367.
DrugBank	DB01615. Aceprometazine. DB00637. Astemizole. DB00719. Azatadine. DB00972. Azelastine. DB00767. Benzquinamide. DB04890. Bepotastine. DB01237. Bromodiphenhydramine. DB00835. Brompheniramine. DB00354. Buclizine. DB00748. Carbinoxamine. DB00341. Cetirizine. DB04837. Chlophedianol. DB01114. Chlorpheniramine. DB01239. Chlorprothixene. DB00568. Cinnarizine. DB00283. Clemastine. DB00363. Clozapine. DB01176. Cyclizine. DB00434. Cyproheptadine. DB01151. Desipramine. DB00967. Desloratadine. DB00405. Dexbrompheniramine. DB00985. Dimenhydrinate. DB01075. Diphenhydramine. DB01146. Diphenylpyraline. DB01142. Doxepin. DB00366. Doxylamine. DB01084. Emedastine. DB00751. Epinastine. DB00950. Fexofenadine. DB04841. Flunarizine. DB00667. Histamine Phosphate. DB00557. Hydroxyzine. DB00920. Ketotifen. DB01106. Levocabastine. DB00455. Loratadine. DB00934. Maprotiline. DB00737. Meclizine. DB01071. Mequitazine. DB00902. Methdilazine. DB01403. Methotrimeprazine. DB06148. Mianserin. DB00370. Mirtazapine. DB00716. Nedocromil. DB00334. Olanzapine. DB00768. Olopatadine. DB01173. Orphenadrine. DB00885. Pemirolast. DB01619. Phenindamine. DB01620. Pheniramine. DB00433. Prochlorperazine. DB00420. Promazine. DB01069. Promethazine. DB00777. Propiomazine. DB01224. Quetiapine. DB00734. Risperidone. DB00342. Terfenadine. DB00372. Thiethylperazine. DB00656. Trazodone. DB01246. Trimeprazine. DB00792. Tripelennamine. DB00427. Triprolidine. DB00246. Ziprasidone.
NextBio	12981.
SOURCE	Search...

Entry information

Entry name

HRH1_HUMAN

Accession

Primary (citable) accession number: P35367
Secondary accession number(s): A8K047, Q6P9E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	March 2, 2010
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.