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P35363 (5HT2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 2A
Short name=5-HT-2
Short name=5-HT-2A
Alternative name(s):
Serotonin receptor 2A
Gene names
Name:Htr2a
Synonyms:Htr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.

Subunit structure

Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Localizes to the postsynaptic thickening of axo-dendritic synapses. Ref.2

Domain

The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4 By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Compara

aging

Inferred from electronic annotation. Source: Compara

cell death

Inferred from electronic annotation. Source: Compara

memory

Inferred from electronic annotation. Source: Compara

negative regulation of potassium ion transport

Inferred from electronic annotation. Source: Compara

negative regulation of synaptic transmission, glutamatergic

Inferred from electronic annotation. Source: Compara

phosphatidylinositol 3-kinase cascade

Inferred from electronic annotation. Source: Compara

phosphatidylinositol biosynthetic process

Inferred from electronic annotation. Source: Compara

phospholipase C-activating serotonin receptor signaling pathway

Inferred from electronic annotation. Source: Compara

positive regulation of MAP kinase activity

Inferred from electronic annotation. Source: Compara

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Compara

positive regulation of vasoconstriction

Inferred from electronic annotation. Source: Compara

regulation of dopamine secretion

Inferred from electronic annotation. Source: Compara

release of sequestered calcium ion into cytosol

Inferred from electronic annotation. Source: Compara

response to drug

Inferred from electronic annotation. Source: Compara

response to stress

Inferred from electronic annotation. Source: Compara

sensory perception of pain

Inferred from electronic annotation. Source: Compara

sleep

Inferred from electronic annotation. Source: Compara

temperature homeostasis

Inferred from electronic annotation. Source: Compara

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Compara

dendritic shaft

Inferred from electronic annotation. Source: Compara

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding

Inferred from electronic annotation. Source: Compara

drug binding

Inferred from electronic annotation. Source: Compara

phosphatidylinositol phospholipase C activity

Inferred from electronic annotation. Source: Compara

serotonin binding

Inferred from sequence orthology PubMed 7582481. Source: MGI

serotonin receptor activity

Inferred from sequence orthology PubMed 7582481. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4714715-hydroxytryptamine receptor 2A
PRO_0000068948

Regions

Topological domain1 – 7575Extracellular By similarity
Transmembrane76 – 9924Helical; Name=1; By similarity
Topological domain100 – 11011Cytoplasmic By similarity
Transmembrane111 – 13222Helical; Name=2; By similarity
Topological domain133 – 14715Extracellular By similarity
Transmembrane148 – 17124Helical; Name=3; By similarity
Topological domain172 – 19120Cytoplasmic By similarity
Transmembrane192 – 21524Helical; Name=4; By similarity
Topological domain216 – 23318Extracellular By similarity
Transmembrane234 – 25421Helical; Name=5; By similarity
Topological domain255 – 32470Cytoplasmic By similarity
Transmembrane325 – 34622Helical; Name=6; By similarity
Topological domain347 – 36216Extracellular By similarity
Transmembrane363 – 38422Helical; Name=7; By similarity
Topological domain385 – 47187Cytoplasmic By similarity
Motif469 – 4713PDZ-binding

Amino acid modifications

Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation541N-linked (GlcNAc...) Potential
Disulfide bond148 ↔ 227 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35363 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: DE763E5617EE8435

FASTA47152,842
        10         20         30         40         50         60 
MEILCEDNIS LSSIPNSLMQ LGDDSRLYPN DFNSRDANTS EASNWTIDAE NRTNLSCEGY 

        70         80         90        100        110        120 
LPPTCLSILH LQEKNWSALL TTVVIILTIA GNILVIMAVS LEKKLQNATN YFLMSLAIAD 

       130        140        150        160        170        180 
MLLGFLVMPV SMLTILYGYR WPLPSKLCAV WIYLDVLFST ASIMHLCAIS LDRYVAIQNP 

       190        200        210        220        230        240 
IHHSRFNSRT KAFLKIIAVW TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF 

       250        260        270        280        290        300 
VAFFIPLTIM VITYFLTIKS LQKEATLCVS DLSTRAKLSS FSFLPQSSLS SEKLFQRSIH 

       310        320        330        340        350        360 
REPGSYAGRR TMQSISNEQK ACKVLGIVFF LFVVMWCPFF ITNIMAVICK ESCNENVIGA 

       370        380        390        400        410        420 
LLNVFVWIGY LSSAVNPLVY TLFNKTYRSA FSRYIQCQYK ENRKPLQLIL VNTIPTLAYK 

       430        440        450        460        470 
SSQLQVGQKK NSQEDAEPTA NDCSMVTLGN QHSEEMCTDN IETVNEKVSC V

« Hide

References

[1]"Gene structure and expression of the mouse 5-HT2 receptor."
Yang W., Chen K., Lan N.C., Gallaher T.K., Shih J.C.
J. Neurosci. Res. 33:196-204(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The serotonin 5-HT2A and 5-HT2C receptors interact with specific sets of PDZ proteins."
Becamel C., Gavarini S., Chanrion B., Alonso G., Galeotti N., Dumuis A., Bockaert J., Marin P.
J. Biol. Chem. 279:20257-20266(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S49542 mRNA. Translation: AAB24369.1.
IPIIPI00117490.
PIRS40689.
RefSeqNP_766400.1. NM_172812.2.
UniGeneMm.214351.

3D structure databases

ProteinModelPortalP35363.
SMRP35363. Positions 38-395.
ModBaseSearch...

Protein-protein interaction databases

IntActP35363. 1 interaction.
STRING10090.ENSMUSP00000047774.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP35363.

Proteomic databases

PRIDEP35363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000036653; ENSMUSP00000047774; ENSMUSG00000034997.
GeneID15558.
KEGGmmu:15558.

Organism-specific databases

CTD3356.
MGIMGI:109521. Htr2a.

Phylogenomic databases

eggNOGNOG247243.
HOGENOMHOG000240378.
HOVERGENHBG107487.
InParanoidP35363.
KOK04157.
OMAFITNVMA.
OrthoDBEOG4TMR24.

Gene expression databases

ArrayExpressP35363.
BgeeP35363.
CleanExMM_HTR2A.
GenevestigatorP35363.

Family and domain databases

InterProIPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP35363.
ChEMBLCHEMBL5377.
NextBio288500.
SOURCESearch...

Entry information

Entry name5HT2A_MOUSE
AccessionPrimary (citable) accession number: P35363
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents