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Protein

Rhodopsin

Gene

rho

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Photoreceptor required for image-forming vision at low light intensity. While most salt water fish species use retinal as chromophore, most freshwater fish use 3-dehydroretinal, or a mixture of retinal and 3-dehydroretinal (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by arrestin and terminates signaling (By similarity).By similarity

Caution

Was originally (PubMed:8327475 and PubMed:8924413) thought to be an ultraviolet-sensitive opsin present in short single cones.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei113Plays an important role in the conformation switch to the active conformationBy similarity1

GO - Molecular functioni

  • 11-cis retinal binding Source: UniProtKB
  • G-protein coupled photoreceptor activity Source: ZFIN
  • photoreceptor activity Source: ZFIN
  • retinal binding Source: ZFIN

GO - Biological processi

  • absorption of visible light Source: ZFIN
  • detection of light stimulus Source: ZFIN
  • protein-chromophore linkage Source: UniProtKB-KW
  • rhodopsin mediated signaling pathway Source: UniProtKB
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionG-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer
Biological processSensory transduction, Vision
LigandChromophore

Enzyme and pathway databases

ReactomeiR-DRE-2453902 The canonical retinoid cycle in rods (twilight vision)
R-DRE-2485179 Activation of the phototransduction cascade
R-DRE-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-DRE-418594 G alpha (i) signalling events
R-DRE-419771 Opsins
R-DRE-5620916 VxPx cargo-targeting to cilium

Protein family/group databases

TCDBi9.A.14.1.12 the g-protein-coupled receptor (gpcr) family

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin
Gene namesi
Name:rho
Synonyms:zfo2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-990415-271 rho

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36ExtracellularCuratedAdd BLAST36
Transmembranei37 – 61Helical; Name=1By similarityAdd BLAST25
Topological domaini62 – 73CytoplasmicCuratedAdd BLAST12
Transmembranei74 – 96Helical; Name=2By similarityAdd BLAST23
Topological domaini97 – 110ExtracellularCuratedAdd BLAST14
Transmembranei111 – 133Helical; Name=3By similarityAdd BLAST23
Topological domaini134 – 152CytoplasmicCuratedAdd BLAST19
Transmembranei153 – 173Helical; Name=4By similarityAdd BLAST21
Topological domaini174 – 202ExtracellularCuratedAdd BLAST29
Transmembranei203 – 224Helical; Name=5By similarityAdd BLAST22
Topological domaini225 – 252CytoplasmicCuratedAdd BLAST28
Transmembranei253 – 274Helical; Name=6By similarityAdd BLAST22
Topological domaini275 – 286ExtracellularCuratedAdd BLAST12
Transmembranei287 – 308Helical; Name=7By similarityAdd BLAST22
Topological domaini309 – 354CytoplasmicCuratedAdd BLAST46

Keywords - Cellular componenti

Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001976541 – 354RhodopsinAdd BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi15N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei296N6-(retinylidene)lysineBy similarity1

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.By similarity
Contains one covalently linked retinal chromophore.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP35359
PRIDEiP35359

Expressioni

Tissue specificityi

Retinal rod photoreceptor cells, predominantly in the outer segments (at protein level) (PubMed:10349976). Retinal rod photoreceptor cells (PubMed:8327475, PubMed:8603882).3 Publications

Developmental stagei

First detected at 51 hours post-fertilization (hpf) in the ventral retina. At 60 hpf, expressed in a strip across the ventrotemporal retina. By 96 hpf, also expressed in the dorsal retina.1 Publication

Gene expression databases

BgeeiENSDARG00000002193
ExpressionAtlasiP35359 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011562

Structurei

3D structure databases

ProteinModelPortaliP35359
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi134 – 136'Ionic lock' involved in activated form stabilizationBy similarity3

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118977
HOGENOMiHOG000253932
HOVERGENiHBG107442
InParanoidiP35359
KOiK04250
OMAiCGKNPFE
OrthoDBiEOG091G0BDA
TreeFamiTF324998

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001760 Opsin
IPR027430 Retinal_BS
IPR000732 Rhodopsin
IPR019477 Rhodopsin_N
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PF10413 Rhodopsin_N, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00238 OPSIN
PR00579 RHODOPSIN
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
PS00238 OPSIN, 1 hit

Sequencei

Sequence statusi: Complete.

P35359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGTEGPAFY VPMSNATGVV RSPYEYPQYY LVAPWAYGLL AAYMFFLIIT
60 70 80 90 100
GFPVNFLTLY VTIEHKKLRT PLNYILLNLA IADLFMVFGG FTTTMYTSLH
110 120 130 140 150
GYFVFGRLGC NLEGFFATLG GEMGLWSLVV LAIERWMVVC KPVSNFRFGE
160 170 180 190 200
NHAIMGVAFT WVMACSCAVP PLVGWSRYIP EGMQCSCGVD YYTRTPGVNN
210 220 230 240 250
ESFVIYMFIV HFFIPLIVIF FCYGRLVCTV KEAAAQQQES ETTQRAEREV
260 270 280 290 300
TRMVIIMVIA FLICWLPYAG VAWYIFTHQG SEFGPVFMTL PAFFAKTSAV
310 320 330 340 350
YNPCIYICMN KQFRHCMITT LCCGKNPFEE EEGASTTASK TEASSVSSSS

VSPA
Length:354
Mass (Da):39,706
Last modified:May 27, 2002 - v2
Checksum:i6C5D49B51059D238
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39 – 40LL → FV (PubMed:8327475).Curated2
Sequence conflicti39 – 40LL → FV (PubMed:8924413).Curated2
Sequence conflicti126W → K (PubMed:8327475).Curated1
Sequence conflicti126W → K (PubMed:8924413).Curated1
Sequence conflicti235A → R (PubMed:8327475).Curated1
Sequence conflicti235A → R (PubMed:8924413).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11014 mRNA Translation: AAA85566.1
AF105152 mRNA Translation: AAD14679.1
AF109368 mRNA Translation: AAD24751.1
AF331797 Genomic DNA Translation: AAK01136.1
BC063938 mRNA Translation: AAH63938.1
PIRiA48191
RefSeqiNP_571159.1, NM_131084.1
UniGeneiDr.354

Genome annotation databases

EnsembliENSDART00000027000; ENSDARP00000011562; ENSDARG00000002193
GeneIDi30295
KEGGidre:30295

Similar proteinsi

Entry informationi

Entry nameiOPSD_DANRE
AccessioniPrimary (citable) accession number: P35359
Secondary accession number(s): Q9PWN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 27, 2002
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

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