P35351 (AGTR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 angiotensin II receptor

Alternative name(s):
Angiotensin II type-2 receptor
Short name=AT2

Gene names

Name:

Agtr2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation By similarity.
Subunit structure	Interacts with MTUS1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Abundant expression in fetal tissues, immature brain, skin wound and atretic ovarian follicles.
Developmental stage	Abundant in whole fetus but decreases rapidly after birth. In adults is highly expressed in the adrenal, present in the brain and uterus but undetectable in the heart.
Post-translational modification	C-terminal Ser or Thr residues may be phosphorylated.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Inferred from electronic annotation. Source: Compara aldosterone secretion Inferred from mutant phenotype PubMed 1566502. Source: RGD behavior Inferred from electronic annotation. Source: Compara brain renin-angiotensin system Inferred from electronic annotation. Source: Compara cell growth involved in cardiac muscle cell development Inferred from mutant phenotype PubMed 21173078. Source: RGD cellular response to dexamethasone stimulus Inferred from direct assay PubMed 17537984. Source: UniProtKB cellular sodium ion homeostasis Inferred from mutant phenotype PubMed 16380540. Source: RGD cerebellar cortex development Inferred from mutant phenotype PubMed 19682991. Source: RGD dopamine biosynthetic process Inferred from mutant phenotype PubMed 20097214. Source: RGD inflammatory response Inferred from direct assay PubMed 21209001. Source: RGD intracellular protein kinase cascade Inferred from electronic annotation. Source: Compara kidney morphogenesis Inferred from mutant phenotype PubMed 18495111. Source: RGD negative regulation of fibroblast proliferation Inferred from mutant phenotype PubMed 22184331. Source: RGD negative regulation of heart rate Inferred from electronic annotation. Source: Compara negative regulation of icosanoid secretion Inferred from mutant phenotype PubMed 18799632. Source: RGD negative regulation of nerve growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of norepinephrine secretion Inferred from direct assay PubMed 18670361. Source: RGD neuron differentiation Traceable author statement PubMed 14664700. Source: RGD positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of branching involved in ureteric bud morphogenesis Inferred from mutant phenotype PubMed 17537984. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype PubMed 22645419. Source: RGD positive regulation of cytokine secretion Inferred from mutant phenotype PubMed 19033105. Source: RGD positive regulation of metanephric glomerulus development Inferred from electronic annotation. Source: Compara positive regulation of nitric-oxide synthase activity Inferred from electronic annotation. Source: Compara positive regulation of phosphoprotein phosphatase activity Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from mutant phenotype PubMed 16380540. Source: RGD positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of vasodilation Inferred from mutant phenotype PubMed 18463192. Source: RGD regulation of metanephros size Inferred from electronic annotation. Source: Compara regulation of systemic arterial blood pressure by circulatory renin-angiotensin Inferred from electronic annotation. Source: Compara regulation of transcription factor import into nucleus Inferred from direct assay PubMed 14657020. Source: RGD renin-angiotensin regulation of aldosterone production Inferred from mutant phenotype PubMed 21318332. Source: RGD response to organic nitrogen Inferred from expression pattern PubMed 16025228. Source: RGD vasodilation by angiotensin involved in regulation of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from direct assay PubMed 14657020. Source: RGD plasma membrane Inferred from direct assay PubMed 14657020. Source: RGD
Molecular_function	G-protein alpha-subunit binding Traceable author statement PubMed 14657020. Source: RGD angiotensin type II receptor activity Traceable author statement PubMed 14664700. Source: RGD peptide hormone binding Inferred from mutant phenotype PubMed 14568903. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Type-2 angiotensin II receptor		PRO_0000069170
Regions
	Topological domain	1 – 45	45	Extracellular Potential
	Transmembrane	46 – 71	26	Helical; Name=1; Potential
	Topological domain	72 – 80	9	Cytoplasmic Potential
	Transmembrane	81 – 102	22	Helical; Name=2; Potential
	Topological domain	103 – 119	17	Extracellular Potential
	Transmembrane	120 – 140	21	Helical; Name=3; Potential
	Topological domain	141 – 160	20	Cytoplasmic Potential
	Transmembrane	161 – 179	19	Helical; Name=4; Potential
	Topological domain	180 – 208	29	Extracellular Potential
	Transmembrane	209 – 234	26	Helical; Name=5; Potential
	Topological domain	235 – 256	22	Cytoplasmic Potential
	Transmembrane	257 – 278	22	Helical; Name=6; Potential
	Topological domain	279 – 297	19	Extracellular Potential
	Transmembrane	298 – 318	21	Helical; Name=7; Potential
	Topological domain	319 – 363	45	Cytoplasmic Potential
Amino acid modifications
	Modified residue	354	1	Phosphoserine; by PKC Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	35 ↔ 290		Ref.6
	Disulfide bond	117 ↔ 195		Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P35351 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 4FE4551A41336650	FASTA	363	41,331
	10 20 30 40 50 60 MKDNFSFAAT SRNITSSLPF DNLNATGTNE SAFNCSHKPA DKHLEAIPVL YYMIFVIGFA 70 80 90 100 110 120 VNIVVVSLFC CQKGPKKVSS IYIFNLAVAD LLLLATLPLW ATYYSYRYDW LFGPVMCKVF 130 140 150 160 170 180 GSFLTLNMFA SIFFITCMSV DRYQSVIYPF LSQRRNPWQA SYVVPLVWCM ACLSSLPTFY 190 200 210 220 230 240 FRDVRTIEYL GVNACIMAFP PEKYAQWSAG IALMKNILGF IIPLIFIATC YFGIRKHLLK 250 260 270 280 290 300 TNSYGKNRIT RDQVLKMAAA VVLAFIICWL PFHVLTFLDA LTWMGIINSC EVIAVIDLAL 310 320 330 340 350 360 PFAILLGFTN SCVNPFLYCF VGNRFQQKLR SVFRVPITWL QGKRETMSCR KSSSLREMDT FVS

References

[1]	"Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition." Kambayashi Y., Bardhan S., Takahashi K., Tsuzuki S., Inui H., Hamakubo T., Inagami T. J. Biol. Chem. 268:24543-24546(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors." Mukoyama M., Nakajima M., Horiuchi M., Sasamura H., Pratt R.E., Dzau V.J. J. Biol. Chem. 268:24539-24542(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Fetus.
[3]	"Cloning, characterization, and genetic mapping of the rat type 2 angiotensin II receptor gene." Koike G., Winer E.S., Horiuchi M., Brown D.M., Szpirer C., Dzau V.J., Jacob H.J. Hypertension 26:998-1002(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Fischer. Tissue: Liver.
[4]	"Cloning, expression and regulation of angiotensin II receptors." Inagami T., Iwai N., Sasaki K., Yamano Y., Bardhan S., Chaki S., Guo D.F., Furuta H., Ohyama K., Kambayashi Y., Takahashi K., Ichiki T. Eur. Heart J. 15:104-107(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Cloning of the rat angiotensin II type 2 receptor gene and identification of its functional promoter region." Kobayashi S., Ohnishi J., Nibu Y., Nishimatsu S., Umemura S., Ishii M., Murakami K., Miyazaki H. Biochim. Biophys. Acta 1262:155-158(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-143. Strain: Sprague-Dawley.
[6]	"Reversible inactivation of AT(2) angiotensin II receptor from cysteine-disulfide bond exchange." Feng Y.H., Saad Y., Karnik S.S. FEBS Lett. 484:133-138(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D16840 mRNA. Translation: BAA04116.1.
U01908 Genomic DNA. Translation: AAC52126.1.
U22663 Genomic DNA. Translation: AAA86509.1.
D43778 Genomic DNA. Translation: BAA07833.1.

IPI

IPI00197641.

PIR

A49092.

RefSeq

NP_036626.1. NM_012494.3.

UniGene

Rn.162367.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G2A	model	-	A	1-363	[»]

ProteinModelPortal

P35351.

ModBase

Search...

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P35351.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000074269; ENSRNOP00000064709; ENSRNOG00000050006.

GeneID

24182.

KEGG

rno:24182.

Organism-specific databases

CTD

186.

RGD

2072. Agtr2.

Phylogenomic databases

GeneTree

ENSGT00700000104167.

HOVERGEN

HBG104998.

KO

K04167.

Gene expression databases

Genevestigator

P35351.

Family and domain databases

InterPro

IPR000147. ATII_AT2_rcpt.
IPR000248. ATII_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00241. ANGIOTENSINR.
PR00636. ANGIOTENSN2R.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P35351.

ChEMBL

CHEMBL257.

NextBio

602529.

Entry information

Entry name

AGTR2_RAT

Accession

Primary (citable) accession number: P35351

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program