P35351 (AGTR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 angiotensin II receptor

Alternative name(s):
Angiotensin II type-2 receptor
Short name=AT2

Gene names

Name:

Agtr2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation By similarity.
Subunit structure	Interacts with MTUS1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Abundant expression in fetal tissues, immature brain, skin wound and atretic ovarian follicles.
Developmental stage	Abundant in whole fetus but decreases rapidly after birth. In adults is highly expressed in the adrenal, present in the brain and uterus but undetectable in the heart.
Post-translational modification	Carboxyl-terminal Ser or Thr residues may be phosphorylated.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to dexamethasone stimulus Inferred from direct assay. Source: UniProtKB cellular sodium ion homeostasis Inferred from mutant phenotype. Source: RGD neuron differentiation Traceable author statement. Source: RGD positive regulation of branching involved in ureteric bud morphogenesis Inferred from mutant phenotype. Source: UniProtKB positive regulation of renal sodium excretion Inferred from mutant phenotype. Source: RGD positive regulation of vasodilation Inferred from mutant phenotype. Source: RGD regulation of transcription factor import into nucleus Inferred from direct assay. Source: RGD renin-angiotensin regulation of aldosterone production Inferred from mutant phenotype. Source: RGD response to organic nitrogen Inferred from expression pattern. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from direct assay. Source: RGD plasma membrane Inferred from direct assay. Source: RGD
Molecular function	G-protein alpha-subunit binding Traceable author statement. Source: RGD angiotensin type II receptor activity Traceable author statement. Source: RGD peptide hormone binding Inferred from mutant phenotype. Source: RGD transcription factor binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Type-2 angiotensin II receptor		PRO_0000069170
Regions
	Topological domain	1 – 45	45	Extracellular Potential
	Transmembrane	46 – 71	26	Helical; Name=1; Potential
	Topological domain	72 – 80	9	Cytoplasmic Potential
	Transmembrane	81 – 102	22	Helical; Name=2; Potential
	Topological domain	103 – 119	17	Extracellular Potential
	Transmembrane	120 – 140	21	Helical; Name=3; Potential
	Topological domain	141 – 160	20	Cytoplasmic Potential
	Transmembrane	161 – 179	19	Helical; Name=4; Potential
	Topological domain	180 – 208	29	Extracellular Potential
	Transmembrane	209 – 234	26	Helical; Name=5; Potential
	Topological domain	235 – 256	22	Cytoplasmic Potential
	Transmembrane	257 – 278	22	Helical; Name=6; Potential
	Topological domain	279 – 297	19	Extracellular Potential
	Transmembrane	298 – 318	21	Helical; Name=7; Potential
	Topological domain	319 – 363	45	Cytoplasmic Potential
Amino acid modifications
	Modified residue	354	1	Phosphoserine; by PKC Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	35 ↔ 290		Ref.6
	Disulfide bond	117 ↔ 195		Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P35351 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 4FE4551A41336650	FASTA	363	41,331
	10 20 30 40 50 60 MKDNFSFAAT SRNITSSLPF DNLNATGTNE SAFNCSHKPA DKHLEAIPVL YYMIFVIGFA 70 80 90 100 110 120 VNIVVVSLFC CQKGPKKVSS IYIFNLAVAD LLLLATLPLW ATYYSYRYDW LFGPVMCKVF 130 140 150 160 170 180 GSFLTLNMFA SIFFITCMSV DRYQSVIYPF LSQRRNPWQA SYVVPLVWCM ACLSSLPTFY 190 200 210 220 230 240 FRDVRTIEYL GVNACIMAFP PEKYAQWSAG IALMKNILGF IIPLIFIATC YFGIRKHLLK 250 260 270 280 290 300 TNSYGKNRIT RDQVLKMAAA VVLAFIICWL PFHVLTFLDA LTWMGIINSC EVIAVIDLAL 310 320 330 340 350 360 PFAILLGFTN SCVNPFLYCF VGNRFQQKLR SVFRVPITWL QGKRETMSCR KSSSLREMDT FVS

References

[1]	"Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition." Kambayashi Y., Bardhan S., Takahashi K., Tsuzuki S., Inui H., Hamakubo T., Inagami T. J. Biol. Chem. 268:24543-24546(1993) [PubMed: 8227011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors." Mukoyama M., Nakajima M., Horiuchi M., Sasamura H., Pratt R.E., Dzau V.J. J. Biol. Chem. 268:24539-24542(1993) [PubMed: 8227010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Fetus.
[3]	"Cloning, characterization, and genetic mapping of the rat type 2 angiotensin II receptor gene." Koike G., Winer E.S., Horiuchi M., Brown D.M., Szpirer C., Dzau V.J., Jacob H.J. Hypertension 26:998-1002(1995) [PubMed: 7490161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Fischer. Tissue: Liver.
[4]	"Cloning, expression and regulation of angiotensin II receptors." Inagami T., Iwai N., Sasaki K., Yamano Y., Bardhan S., Chaki S., Guo D.F., Furuta H., Ohyama K., Kambayashi Y., Takahashi K., Ichiki T. Eur. Heart J. 15:104-107(1994) [PubMed: 7713098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Cloning of the rat angiotensin II type 2 receptor gene and identification of its functional promoter region." Kobayashi S., Ohnishi J., Nibu Y., Nishimatsu S., Umemura S., Ishii M., Murakami K., Miyazaki H. Biochim. Biophys. Acta 1262:155-158(1995) [PubMed: 7599191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-143. Strain: Sprague-Dawley.
[6]	"Reversible inactivation of AT(2) angiotensin II receptor from cysteine-disulfide bond exchange." Feng Y.H., Saad Y., Karnik S.S. FEBS Lett. 484:133-138(2000) [PubMed: 11068047] [Abstract] Cited for: DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D16840 mRNA. Translation: BAA04116.1.
U01908 Genomic DNA. Translation: AAC52126.1.
U22663 Genomic DNA. Translation: AAA86509.1.
D43778 Genomic DNA. Translation: BAA07833.1.

IPI

IPI00197641.

PIR

A49092.

RefSeq

NP_036626.1. NM_012494.3.

UniGene

Rn.162367.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G2A	model	-	A	1-363	[»]

ProteinModelPortal

P35351.

ModBase

Search...

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P35351.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

24182.

KEGG

rno:24182.

UCSC

NM_012494. rat.

Organism-specific databases

CTD

186.

RGD

2072. Agtr2.

Phylogenomic databases

HOVERGEN

HBG104998.

Gene expression databases

Genevestigator

P35351.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR000147. ATII_AT2_rcpt.
IPR000248. ATII_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]

KO

K04167.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00241. ANGIOTENSINR.
PR00636. ANGIOTENSN2R.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

602529.

Entry information

Entry name

AGTR2_RAT

Accession

Primary (citable) accession number: P35351

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program