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P35349 (GRM6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 6

Short name=mGluR6
Gene names
Name:Grm6
Synonyms:Gprc1f, Mglur6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length871 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity By similarity. Signaling stimulates TRPM1 channel activity and Ca2+ uptake. Required for normal vision. Ref.1

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Golgi apparatus membrane; Multi-pass membrane protein By similarity. Cell projectiondendrite By similarity. Note: Subject to trafficking from the endoplasmic reticulum to the Golgi apparatus and then to the cell membrane. Detected at dendritic tips of bipolar cells By similarity.

Tissue specificity

Restricted expression in the inner nuclear layer of the retina. Ref.1

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Biological processSensory transduction
Vision
   Cellular componentCell membrane
Cell projection
Endoplasmic reticulum
Golgi apparatus
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled glutamate receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway

Inferred from direct assay Ref.1. Source: RGD

detection of light stimulus involved in visual perception

Inferred from sequence or structural similarity. Source: UniProtKB

locomotory behavior

Inferred from electronic annotation. Source: Ensembl

positive regulation of calcium ion import

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

retina development in camera-type eye

Inferred from electronic annotation. Source: Ensembl

synaptic transmission

Inferred from expression pattern Ref.1. Source: RGD

   Cellular_componentGolgi membrane

Inferred from sequence or structural similarity. Source: UniProtKB

dendrite

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Traceable author statement Ref.1. Source: RGD

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

new growing cell tip

Inferred from electronic annotation. Source: Ensembl

presynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionadenylate cyclase inhibiting G-protein coupled glutamate receptor activity

Inferred from direct assay Ref.1. Source: RGD

glutamate receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

group III metabotropic glutamate receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 871848Metabotropic glutamate receptor 6
PRO_0000012937

Regions

Topological domain24 – 579556Extracellular Potential
Transmembrane580 – 60223Helical; Name=1; Potential
Topological domain603 – 61614Cytoplasmic Potential
Transmembrane617 – 63721Helical; Name=2; Potential
Topological domain638 – 64811Extracellular Potential
Transmembrane649 – 66719Helical; Name=3; Potential
Topological domain668 – 69124Cytoplasmic Potential
Transmembrane692 – 71221Helical; Name=4; Potential
Topological domain713 – 74230Extracellular Potential
Transmembrane743 – 76422Helical; Name=5; Potential
Topological domain765 – 77713Cytoplasmic Potential
Transmembrane778 – 80023Helical; Name=6; Potential
Topological domain801 – 81313Extracellular Potential
Transmembrane814 – 83926Helical; Name=7; Potential
Topological domain840 – 87132Cytoplasmic Potential
Region169 – 1713Glutamate binding By similarity

Sites

Binding site1481Glutamate By similarity
Binding site2191Glutamate By similarity
Binding site3011Glutamate By similarity
Binding site3941Glutamate By similarity

Amino acid modifications

Glycosylation2901N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation4731N-linked (GlcNAc...) Potential
Glycosylation5611N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 93 By similarity
Disulfide bond238 ↔ 530 By similarity
Disulfide bond361 ↔ 377 By similarity
Disulfide bond417 ↔ 424 By similarity
Disulfide bond512 ↔ 531 By similarity
Disulfide bond516 ↔ 534 By similarity
Disulfide bond537 ↔ 549 By similarity
Disulfide bond552 ↔ 565 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35349 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 9F70B4D6A13B186D

FASTA87195,089
        10         20         30         40         50         60 
MGRLPVLLLW LAWWLSQAGI ACGAGSVRLA GGLTLGGLFP VHARGAAGRA CGALKKEQGV 

        70         80         90        100        110        120 
HRLEAMLYAL DRVNADPELL PGVRLGARLL DTCSRDTYAL EQALSFVQAL IRGRGDGDEA 

       130        140        150        160        170        180 
SVRCPGGVPP LRSAPPERVV AVVGASASSV SIMVANVLRL FAIPQISYAS TAPELSDSTR 

       190        200        210        220        230        240 
YDFFSRVVPP DSYQAQAMVD IVRALGWNYV STLASEGNYG ESGVEAFVQI SREAGGVCIA 

       250        260        270        280        290        300 
QSIKIPREPK PGEFHKVIRR LMETPNARGI IIFANEDDIR RVLEATRQAN LTGHFLWVGS 

       310        320        330        340        350        360 
DSWGSKISPI LNLEEEAVGA ITILPKRASI DGFDQYFMTR SLENNRRNIW FAEFWEENFN 

       370        380        390        400        410        420 
CKLTSSGGQS DDSTRKCTGE ERIGQDSAYE QEGKVQFVID AVYAIAHALH SMHQALCPGH 

       430        440        450        460        470        480 
TGLCPAMEPT DGRTLLHYIR AVRFNGSAGT PVMFNENGDA PGRYDIFQYQ ATNGSASSGG 

       490        500        510        520        530        540 
YQAVGQWAEA LRLDMEVLRW SGDPHEVPPS QCSLPCGPGE RKKMVKGVPC CWHCEACDGY 

       550        560        570        580        590        600 
RFQVDEFTCE ACPGDMRPTP NHTGCRPTPV VRLTWSSPWA ALPLLLAVLG IMATTTIMAT 

       610        620        630        640        650        660 
FMRHNDTPIV RASGRELSYV LLTGIFLIYA ITFLMVAEPC AAICAARRLL LGLGTTLSYS 

       670        680        690        700        710        720 
ALLTKTNRIY RIFEQGKRSV TPPPFISPTS QLVITFGLTS LQVVGVIAWL GAQPPHSVID 

       730        740        750        760        770        780 
YEEQRTVDPE QARGVLKCDM SDLSLIGCLG YSLLLMVTCT VYAIKARGVP ETFNEAKPIG 

       790        800        810        820        830        840 
FTMYTTCIIW LAFVPIFFGT AQSAEKIYIQ TTTLTVSLSL SASVSLGMLY VPKTYVILFH 

       850        860        870 
PEQNVQKRKR SLKKTSTMAA PPQNENAEDA K 

« Hide

References

[1]"Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4-phosphonobutyrate."
Nakajima Y., Iwakabe H., Akazawa C., Nawa H., Shigemoto R., Nakanishi S.
J. Biol. Chem. 268:11868-11873(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Retina.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13963 mRNA. Translation: BAA03066.1.
PIRA46742.
RefSeqNP_075209.1. NM_022920.1.
UniGeneRn.44615.

3D structure databases

ProteinModelPortalP35349.
SMRP35349. Positions 26-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246584. 5 interactions.
IntActP35349. 2 interactions.
MINTMINT-1777333.
STRING10116.ENSRNOP00000000249.

Chemistry

BindingDBP35349.
ChEMBLCHEMBL3842.
GuidetoPHARMACOLOGY294.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP35349.

Proteomic databases

PaxDbP35349.
PRIDEP35349.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000249; ENSRNOP00000000249; ENSRNOG00000000233.
GeneID24419.
KEGGrno:24419.
UCSCRGD:2747. rat.

Organism-specific databases

CTD2916.
RGD2747. Grm6.

Phylogenomic databases

eggNOGNOG295200.
GeneTreeENSGT00750000117581.
HOGENOMHOG000218635.
HOVERGENHBG107965.
InParanoidP35349.
KOK04608.
OMAVCIAQSI.
OrthoDBEOG7Z0JXG.
PhylomeDBP35349.
TreeFamTF313240.

Gene expression databases

GenevestigatorP35349.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000112. GPCR_3_mtglu_rcpt_6.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01056. MTABOTROPC6R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603271.

Entry information

Entry nameGRM6_RAT
AccessionPrimary (citable) accession number: P35349
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries