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P35343 (CXCR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-X-C chemokine receptor type 2

Short name=CXC-R2
Short name=CXCR-2
Alternative name(s):
GRO/MGSA receptor
High affinity interleukin-8 receptor B
Short name=IL-8R B
CD_antigen=CD182
Gene names
Name:Cxcr2
Synonyms:Cmkar2, Gpcr16, Il8rb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Ref.10

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylated upon ligand binding; which is required for desensitization By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacute inflammatory response to antigenic stimulus

Inferred from electronic annotation. Source: Ensembl

cellular defense response

Inferred from electronic annotation. Source: Ensembl

chemokine-mediated signaling pathway

Inferred from direct assay PubMed 16618742. Source: GOC

chemotaxis

Traceable author statement PubMed 12470609. Source: MGI

metanephric tubule morphogenesis

Inferred from electronic annotation. Source: Ensembl

midbrain development

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 16618742. Source: MGI

negative regulation of neutrophil apoptotic process

Inferred from electronic annotation. Source: Ensembl

neutrophil activation

Inferred from electronic annotation. Source: Ensembl

neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

positive regulation of neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of vascular permeability

Inferred from electronic annotation. Source: Ensembl

receptor internalization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mast cell granule

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionchemokine receptor activity

Inferred from direct assay PubMed 16618742. Source: MGI

interleukin-8 receptor activity

Traceable author statement PubMed 8631787. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359C-X-C chemokine receptor type 2
PRO_0000069339

Regions

Topological domain1 – 4747Extracellular Potential
Transmembrane48 – 7427Helical; Name=1; Potential
Topological domain75 – 839Cytoplasmic Potential
Transmembrane84 – 10421Helical; Name=2; Potential
Topological domain105 – 11915Extracellular Potential
Transmembrane120 – 14122Helical; Name=3; Potential
Topological domain142 – 16221Cytoplasmic Potential
Transmembrane163 – 18220Helical; Name=4; Potential
Topological domain183 – 20725Extracellular Potential
Transmembrane208 – 23023Helical; Name=5; Potential
Topological domain231 – 25020Cytoplasmic Potential
Transmembrane251 – 27222Helical; Name=6; Potential
Topological domain273 – 29321Extracellular Potential
Transmembrane294 – 31421Helical; Name=7; Potential
Topological domain315 – 35945Cytoplasmic Potential

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Disulfide bond118 ↔ 195 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35343 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 74BD166E9B679F88

FASTA35940,425
        10         20         30         40         50         60 
MGEFKVDKFN IEDFFSGDLD IFNYSSGMPS ILPDAVPCHS ENLEINSYAV VVIYVLVTLL 

        70         80         90        100        110        120 
SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WTFGSTLCKI 

       130        140        150        160        170        180 
FSYVKEVTFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCIAMWL LSVILALPIL 

       190        200        210        220        230        240 
ILRNPVKVNL STLVCYEDVG NNTSRLRVVL RILPQTFGFL VPLLIMLFCY GFTLRTLFKA 

       250        260        270        280        290        300 
HMGQKHRAMR VIFAVVLVFL LCWLPYNLVL FTDTLMRTKL IKETCERRDD IDKALNATEI 

       310        320        330        340        350 
LGFLHSCLNP IIYAFIGQKF RHGLLKIMAT YGLVSKEFLA KEGRPSFVSS SSANTSTTL 

« Hide

References

« Hide 'large scale' references
[1]"The murine homologue of the human interleukin-8 receptor type B maps near the Ity-Lsh-Bcg disease resistance locus."
Cerretti D.P., Nelson N., Kozlosky C.J., Morrissey P.J., Copeland N.G., Gilbert D.J., Jenkins N.A., Dosik J.K., Mock B.A.
Genomics 18:410-413(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The N-terminus of interleukin-8 (IL-8) receptor confers high affinity binding to human IL-8."
Suzuki H., Prado G.N., Wilkinson N., Navarro J.
J. Biol. Chem. 269:18263-18266(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The murine interleukin 8 type B receptor homologue and its ligands. Expression and biological characterization."
Bozic C.R., Gerard N.P., von Uexkull-Guldenband C., Kolakowski L.F. Jr., Conklyn M.J., Breslow R., Showell H.J., Gerard C.
J. Biol. Chem. 269:29355-29358(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[4]"Chemokine binding and activities mediated by the mouse IL-8 receptor."
Lee J., Cacalano G., Camerato T., Toy K., Moore M.W., Wood W.I.
J. Immunol. 155:2158-2164(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[5]"Cloning of a cDNA encoding a mouse homolog of the interleukin-8 receptor."
Harada A., Kuno K., Nomura H., Mukaida N., Murakami S., Matsushima K.
Gene 142:297-300(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Inner ear.
[7]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Trophoblast.
[9]"Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors."
Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A.
Genomics 18:175-184(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 145-258.
Tissue: Testis.
[10]"IL-10-conditioned dendritic cells, decommissioned for recruitment of adaptive immunity, elicit innate inflammatory gene products in response to danger signals."
Nolan K.F., Strong V., Soler D., Fairchild P.J., Cobbold S.P., Croxton R., Gonzalo J.-A., Rubio A., Wells M., Waldmann H.
J. Immunol. 172:2201-2209(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23637 Genomic DNA. Translation: AAA39305.1.
L26549 Genomic DNA. Translation: AAL31314.1.
L13239 Genomic DNA. Translation: AAA62109.1.
U31207 Genomic DNA. Translation: AAC52239.1.
D17630 mRNA. Translation: BAA04536.1.
AK158234 mRNA. Translation: BAE34417.1.
CH466548 Genomic DNA. Translation: EDL00307.1.
BC051677 mRNA. Translation: AAH51677.1.
L20337 mRNA. Translation: AAA16853.1.
PIRA48921.
RefSeqNP_034039.1. NM_009909.3.
XP_006495701.1. XM_006495638.1.
UniGeneMm.234466.

3D structure databases

ProteinModelPortalP35343.
SMRP35343. Positions 37-332.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000027372.

Chemistry

GuidetoPHARMACOLOGY69.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP35343.

Proteomic databases

PRIDEP35343.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027372; ENSMUSP00000027372; ENSMUSG00000026180.
ENSMUST00000106899; ENSMUSP00000102512; ENSMUSG00000026180.
GeneID12765.
KEGGmmu:12765.
UCSCuc007bll.1. mouse.

Organism-specific databases

CTD3579.
MGIMGI:105303. Cxcr2.

Phylogenomic databases

eggNOGNOG146068.
GeneTreeENSGT00730000110292.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidQ53X27.
KOK05050.
OMALFRRTIY.
OrthoDBEOG7ZPNK9.
PhylomeDBP35343.
TreeFamTF330966.

Gene expression databases

BgeeP35343.
CleanExMM_IL8RB.
GenevestigatorP35343.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000057. Chemokine_CXCR2.
IPR000174. Chemokine_CXCR_1/2.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PTHR24227:SF39. PTHR24227:SF39. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00427. INTRLEUKIN8R.
PR00573. INTRLEUKN8BR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio282122.
PROP35343.
SOURCESearch...

Entry information

Entry nameCXCR2_MOUSE
AccessionPrimary (citable) accession number: P35343
Secondary accession number(s): Q53X27
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries