P35343 (CXCR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-X-C chemokine receptor type 2
Short name=CXC-R2
Short name=CXCR-2

Alternative name(s):
GRO/MGSA receptor
High affinity interleukin-8 receptor B
Short name=IL-8R B
CD_antigen=CD182

Gene names

Name:	Cxcr2
Synonyms:	Cmkar2, Gpcr16, Il8rb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated upon ligand binding; which is required for desensitization By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from direct assay PubMed 16618742. Source: MGI cellular defense response Inferred from electronic annotation. Source: Compara chemotaxis Traceable author statement PubMed 12470609. Source: MGI elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara negative regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: InterPro neutrophil activation Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara receptor internalization Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mast cell granule Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	interleukin-8 receptor activity Traceable author statement PubMed 8631787. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	C-X-C chemokine receptor type 2		PRO_0000069339
Regions
	Topological domain	1 – 47	47	Extracellular Potential
	Transmembrane	48 – 74	27	Helical; Name=1; Potential
	Topological domain	75 – 83	9	Cytoplasmic Potential
	Transmembrane	84 – 104	21	Helical; Name=2; Potential
	Topological domain	105 – 119	15	Extracellular Potential
	Transmembrane	120 – 141	22	Helical; Name=3; Potential
	Topological domain	142 – 162	21	Cytoplasmic Potential
	Transmembrane	163 – 182	20	Helical; Name=4; Potential
	Topological domain	183 – 207	25	Extracellular Potential
	Transmembrane	208 – 230	23	Helical; Name=5; Potential
	Topological domain	231 – 250	20	Cytoplasmic Potential
	Transmembrane	251 – 272	22	Helical; Name=6; Potential
	Topological domain	273 – 293	21	Extracellular Potential
	Transmembrane	294 – 314	21	Helical; Name=7; Potential
	Topological domain	315 – 359	45	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 195		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35343 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 74BD166E9B679F88	FASTA	359	40,425
	10 20 30 40 50 60 MGEFKVDKFN IEDFFSGDLD IFNYSSGMPS ILPDAVPCHS ENLEINSYAV VVIYVLVTLL 70 80 90 100 110 120 SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WTFGSTLCKI 130 140 150 160 170 180 FSYVKEVTFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCIAMWL LSVILALPIL 190 200 210 220 230 240 ILRNPVKVNL STLVCYEDVG NNTSRLRVVL RILPQTFGFL VPLLIMLFCY GFTLRTLFKA 250 260 270 280 290 300 HMGQKHRAMR VIFAVVLVFL LCWLPYNLVL FTDTLMRTKL IKETCERRDD IDKALNATEI 310 320 330 340 350 LGFLHSCLNP IIYAFIGQKF RHGLLKIMAT YGLVSKEFLA KEGRPSFVSS SSANTSTTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine homologue of the human interleukin-8 receptor type B maps near the Ity-Lsh-Bcg disease resistance locus." Cerretti D.P., Nelson N., Kozlosky C.J., Morrissey P.J., Copeland N.G., Gilbert D.J., Jenkins N.A., Dosik J.K., Mock B.A. Genomics 18:410-413(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The N-terminus of interleukin-8 (IL-8) receptor confers high affinity binding to human IL-8." Suzuki H., Prado G.N., Wilkinson N., Navarro J. J. Biol. Chem. 269:18263-18266(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The murine interleukin 8 type B receptor homologue and its ligands. Expression and biological characterization." Bozic C.R., Gerard N.P., von Uexkull-Guldenband C., Kolakowski L.F. Jr., Conklyn M.J., Breslow R., Showell H.J., Gerard C. J. Biol. Chem. 269:29355-29358(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[4]	"Chemokine binding and activities mediated by the mouse IL-8 receptor." Lee J., Cacalano G., Camerato T., Toy K., Moore M.W., Wood W.I. J. Immunol. 155:2158-2164(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[5]	"Cloning of a cDNA encoding a mouse homolog of the interleukin-8 receptor." Harada A., Kuno K., Nomura H., Mukaida N., Murakami S., Matsushima K. Gene 142:297-300(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Inner ear.
[7]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Trophoblast.
[9]	"Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors." Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A. Genomics 18:175-184(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 145-258. Tissue: Testis.
[10]	"IL-10-conditioned dendritic cells, decommissioned for recruitment of adaptive immunity, elicit innate inflammatory gene products in response to danger signals." Nolan K.F., Strong V., Soler D., Fairchild P.J., Cobbold S.P., Croxton R., Gonzalo J.-A., Rubio A., Wells M., Waldmann H. J. Immunol. 172:2201-2209(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23637 Genomic DNA. Translation: AAA39305.1. L26549 Genomic DNA. Translation: AAL31314.1. L13239 Genomic DNA. Translation: AAA62109.1. U31207 Genomic DNA. Translation: AAC52239.1. D17630 mRNA. Translation: BAA04536.1. AK158234 mRNA. Translation: BAE34417.1. CH466548 Genomic DNA. Translation: EDL00307.1. BC051677 mRNA. Translation: AAH51677.1. L20337 mRNA. Translation: AAA16853.1.
IPI	IPI00117446.
PIR	A48921.
RefSeq	NP_034039.1. NM_009909.3.
UniGene	Mm.234466.
3D structure databases
ProteinModelPortal	P35343.
SMR	P35343. Positions 37-332.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000027372.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P35343.
Proteomic databases
PRIDE	P35343.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027372; ENSMUSP00000027372; ENSMUSG00000026180. ENSMUST00000106899; ENSMUSP00000102512; ENSMUSG00000026180.
GeneID	12765.
KEGG	mmu:12765.
UCSC	uc007bll.1. mouse.
Organism-specific databases
CTD	3579.
MGI	MGI:105303. Cxcr2.
Phylogenomic databases
eggNOG	NOG146068.
GeneTree	ENSGT00640000091250.
HOGENOM	HOG000234122.
HOVERGEN	HBG106917.
InParanoid	Q53X27.
KO	K05050.
OMA	THGLISK.
OrthoDB	EOG4M0F2B.
Gene expression databases
Bgee	P35343.
CleanEx	MM_IL8RB.
Genevestigator	P35343.
Family and domain databases
InterPro	IPR000174. Chemokine_CXCR. IPR000057. Chemokine_CXCR2. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00427. INTRLEUKIN8R. PR00573. INTRLEUKN8BR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	282122.
SOURCE	Search...

Entry information

Entry name

CXCR2_MOUSE

Accession

Primary (citable) accession number: P35343
Secondary accession number(s): Q53X27

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program