P35341 (L_SV41) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Simian virus 41 (SV41) [Complete proteome]

Taxonomic identifier

11228 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Simiiformes [TaxID: 314293]

Protein attributes

Sequence length	2269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxovirus L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2269	2269	RNA-directed RNA polymerase L		PRO_0000142741
Regions
	Domain	666 – 850	185	RdRp catalytic
	Nucleotide binding	1815 – 1824	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P35341 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 3BD60C14AA161F5B	FASTA	2,269	256,432
	10 20 30 40 50 60 MAASADILLP EVHLNSPIVK HKLVYFLLLG KLPHNLSEDE ITPLHNQNWD QIAHEESNLS 70 80 90 100 110 120 ERLFAVRSEL TRRIAQLRAT RWRSEIAVLL WPNSLPYLCT FKPYNRLNTI DEWNKLVSAA 130 140 150 160 170 180 SNILSSPLSK CMQDISTKLI GRTNLFSRSQ SRPGQSADNT ITLNKIAAVW ADNKWQPLVS 190 200 210 220 230 240 LWLTIKYQMR QMIANQSKRT CSELVYVVDT RSGIIIITPE LVTCFDKDHS VLMYFTFEMV 250 260 270 280 290 300 LMISDLFEGR MNVTALCTVS NYLSPLLSRI ERLFDIVDHL AHLLGDNVYK IIASLESLVY 310 320 330 340 350 360 GCLQLHDPVI DLAGTFYSFV AQEIVDGLQQ GNILSPEEAY TVTEQLLECF SGLSPDLTAE 370 380 390 400 410 420 LLCLMRLWGH PNLTAAQAAK KVRDTMCAGK VLDFQIIMKT LAFFHTILIN GYRRKKNGIW 430 440 450 460 470 480 PPLSLPGNAS KSLIELHHDN SEISYEYTLR HWKELSLIEF KKCFDFDPGE ELSIFMKDKA 490 500 510 520 530 540 ISAPKEDWMS VFRKSLIKQR HQRHHIPMPN PFNRRLLLNF IEDPSFDPAK ELEYVTSGEY 550 560 570 580 590 600 LRDPHFCASY SLKEKEIKPD GRIFAKLTNR MRSCQVIAEA LLANHAGKLM KENGVVMNQI 610 620 630 640 650 660 SLTKSLLTMS QIGLISEKAQ RYTRDNIALG ALFSKGQRTR AHPQTQLSSI DSSQNRELPD 670 680 690 700 710 720 DSLELSACFI TTDLTKYCPQ WRYQTIIPFA KTLNRMYGVP HLFEWIHLRL LRSTLYVGDP 730 740 750 760 770 780 FNPPADTSVF DLDQVLNGDI FIVSPKGGIE GLCQKMWTMI SISVIILSSA ESKTRVMSMV 790 800 810 820 830 840 QGDNQAIAVT TKVPRSVPLT EKRNLAYNAS KLFFDRLKHN NFGLGHQLKA QETIISSQFF 850 860 870 880 890 900 IYSKRVFYQG RILTQALKNA SKLCLTADVL GECTQASCSN AATTIMRLTE NGVEKDVCYM 910 920 930 940 950 960 LNVYQAIRQL CFDLYYPQYS IPGEQISQHY LKHPSIVARL VILPSQLGGL NYLSCSRLFN 970 980 990 1000 1010 1020 RNIGDPLGTA VADLKRLIKC GALESWVLSN LLSRKPGTGS WATLAADPYS LNIDYLYPPT 1030 1040 1050 1060 1070 1080 TILKRHTQNT LMEVCKNPML KGVFTDNARE EENNLAKFLL DRDIVLPRVA HIVIEQSSVG 1090 1100 1110 1120 1130 1140 RKKQIQGFFD TTRTIMRKSF EIKPLSSKRT LSVIECNINY LAYNFNIIHH PNPIPGYLQC 1150 1160 1170 1180 1190 1200 ITTDNCSVDI ARSLRKLSWS SLLNGRTLEG LETPDPIEVV NGALVIGVGE CDYCMQGDTK 1210 1220 1230 1240 1250 1260 FTWFFLPKGI EIDGDPEKNP PIRVPYVGSK TEERRVASMA YVKGATSSLK AALRGAGVFI 1270 1280 1290 1300 1310 1320 WAYGDTDANW DDALDLANTR VQISKEQLQA LTPLPTSANI THRLDDGATT IKFTPASSYA 1330 1340 1350 1360 1370 1380 FSSYTHISND QQYLEVDNRV VDSNIIYQQL MITGLGIIET YNNPPIRTSS EELTLHLHTS 1390 1400 1410 1420 1430 1440 SSCCIRPVDG CIICESPSLL PQLTVPYTNP FVYDPNPLAD YEIAHLDYLS YQARIGSIEH 1450 1460 1470 1480 1490 1500 YSLQDRIDLL AHLTAKQMIN SIIGLDESVS LLNDAVVTSD YTNNWISECS YTKIDLVFKM 1510 1520 1530 1540 1550 1560 MAWNLLLELS FQMYYLRITT WSNIFDYTYM TLRRIPGNAL TNIAATISHP KLLRRAMNLD 1570 1580 1590 1600 1610 1620 VITPVHAPYL ASLDYIKLSI DAIQWGIKQV LADLHNGIDY EILILSEDSL ELSDRAMNLI 1630 1640 1650 1660 1670 1680 ARKLTLLALI QGNQLVLPKI KGLSPDEKCL VLTEHLMSEY QLLLLDDAEL SKRSYNLTNP 1690 1700 1710 1720 1730 1740 RITAFPSNNF YLTRKVLNSI RDSEEGQYLI GAYYDSFQQM ETDIILHSTL IAPYDTSETL 1750 1760 1770 1780 1790 1800 TKFDLCISLF PHDDNLEKYP LPVDHDSQSA VSTIVPGPPI HHVLRPLGVS STSWYKGLSY 1810 1820 1830 1840 1850 1860 VRYLELCKVP TGDHLYLAEG SGASMSLVEM LIPGQKVYYN SLFSSGENPP QRNYAPLPTQ 1870 1880 1890 1900 1910 1920 FVQSVPYKLW QADLTNKEGI IEDFIPLWNG NGAVTDLSNK DCVAFIIHKV GAEQASLVHV 1930 1940 1950 1960 1970 1980 DLESTANLNQ QSLSRSQIHA LIIATTVLKR GGFLVYKTSW LPFSRLSQLA CVLWSFFDKI 1990 2000 2010 2020 2030 2040 TMIRSSYSDP NSHEIYLVCR LAADFKTIGF SAALASAIAI AIHGDGFTTI HPDVVSNYWQ 2050 2060 2070 2080 2090 2100 HHLENVGRVG KAIDDVIDGV STNFYSGDNG LILRCGGTPS SRKWLDIDLL PTFSSLQETL 2110 2120 2130 2140 2150 2160 VNLVTVHLKE IIEIQTSSME DYTSLLFTPY NIGSVGKIRT IVKLILERSL MYVIRNWLVM 2170 2180 2190 2200 2210 2220 PSSYQDSVRQ DLELGSFRLS SVLQEDTFWK LTENRKYLAS QLTRDYITTF FTTHSILPIH 2230 2240 2250 2260 RSYQKRIWKA LGSVIYCSEV PGESQQNWDS IPLVYEEDQI ERGIDGEEL

References

[1]

"Nucleotide sequence analysis of the simian virus 41 gene encoding the large (L) protein and construction of a phylogenetic tree for the L proteins of paramyxoviruses." Ogawa M., Mutsuga N., Tsurudome M., Kawano M., Matsumura H., Kusagawa S., Komada H., Nishio M., Ito Y. J. Gen. Virol. 73:2743-2750(1992) [PubMed: 1328485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Toshiba/Chanock.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64275 Genomic RNA. Translation: CAA45569.1.
PIR	JQ1750.
RefSeq	YP_138510.1. NC_006428.1.
3D structure databases
ProteinModelPortal	P35341.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3159460.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR016269. RNA-dir_pol_paramyxovirus. IPR024352. RNA_pol_cap_MeTfrase. IPR002877. rRNA_MeTrfase_RrmJ/FtsJ. [Graphical view]
Pfam	PF01728. FtsJ. 1 hit. PF12803. G-7-MTase. 1 hit. PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_SV41

Accession

Primary (citable) accession number: P35341

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program