Reviewed, UniProtKB/Swiss-Prot P35339 (PGLR3_MAIZE)

Names and origin

Protein names

Recommended name:
    Exopolygalacturonase
      Short name=ExoPG
    EC=3.2.1.67
Alternative name(s):
    Pectinase
    Galacturan 1,4-alpha-galacturonidase

Gene names

Name:

PG2C

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.
Catalytic activity	((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.
Subcellular location	Secreted. Secreted › cell wall.
Tissue specificity	Pollen.
Developmental stage	Late stages of pollen development.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galacturan 1,4-alpha-galacturonidase activity Inferred from electronic annotation. Source: EC polygalacturonase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 410	388	Exopolygalacturonase		PRO_0000024808
Sites
	Active site	233	1	Proton donor By similarity
	Active site	256	1	By similarity
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	246	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P35339-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5BD1310F588AE7CA	FASTA	410	43,297
	10 20 30 40 50 60 MACIDNAMRA LFLLALFCVV HGEKAKSKDN DAKASGPGGS FDITKLGASG NGKTDSTKAV 70 80 90 100 110 120 QEAWASACGG TGKQTILIPK GDFLVGPLNF TGPCKGDVTI QVNGNLLATT DLSQYKDHGN 130 140 150 160 170 180 WIEILRVDNL VITGKGKLDG QGPAVWSKNS CVKKYDCKIL PNSLVMDFVN NGEVSGITLL 190 200 210 220 230 240 NSKFFHMNMY KCKDMLIKDV NVTAPGDSPN TDGIHMGDSS GVTITNTVIG VGDDCISIGP 250 260 270 280 290 300 GTSKVNITGV TCGPGHGISI GSLGRYKDEK DVTDINVKDC TLKKTANGVR IKAYEDAASV 310 320 330 340 350 360 LTASKIHYEN IKMEDSGYPI IIDMKYCPNK LCTANGASKV TVKDVTFKNI TGTSSTPEAV 370 380 390 400 410 NLLCTAKIPC TGVTMDDVNI KYSGTNNKTM AVCKNAKGSA KGCLKELACF

References

[1]	"Characterization of a multigene family encoding an exopolygalacturonase in maize." Barakate A., Martin W., Quigley F., Mache R. J. Mol. Biol. 229:797-801(1993) [PubMed: 8433375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Missouri 17. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66422 Genomic DNA. Translation: CAA47052.1.
PIR	S30067.
UniGene	Zm.36659
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Organism-specific databases
Gramene	P35339.
MaizeGDB	65847.
Enzyme and pathway databases
BRENDA	3.2.1.67. 289.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR3_MAIZE

Accession

Primary (citable) accession number: P35339

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)