Reviewed, UniProtKB/Swiss-Prot P35337 (PGLR_BRANA)

Names and origin

Protein names	Recommended name:     Polygalacturonase       Short name=PG     EC=3.2.1.15 Alternative name(s):     Pectinase
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function in depolymerizing pectin during pollen development, germination, and tube growth.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Tissue specificity	Pollen.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 4 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 397	375	Polygalacturonase		PRO_0000024801
Sites
	Active site	210	1	Proton donor By similarity
	Active site	233	1	By similarity
Amino acid modifications
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P35337-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 94522440A7134260	FASTA	397	42,447
	10 20 30 40 50 60 MGSYLGIYTI LVLCLLGYSA NAEVFTAGGP PNSDITAAVL KAFTSACQAP APSQVLIPKG 70 80 90 100 110 120 DFKLGETVMT GPCKSPIEFT LQGNVKTDGG STQGKDRWVV FEKINGFKLN GGGTFDGEGN 130 140 150 160 170 180 AAWKANNCHK TFECKKLPIS VRFDFVDNAE IKDVTSLDAK NFHFNVISGK NMTFDNIKII 190 200 210 220 230 240 APAESPNTDG IHLGRCEGVK ILNTKIATGD DCISVGDGMK NLLIEKVVCG PGHGISVGSL 250 260 270 280 290 300 GRYGWEQDVT DITVKNCTLE GTSNGLRIKT WPSAACTTTA AGIHFEDIIL NKVSNPILID 310 320 330 340 350 360 QEYCPWNQCN KNKPSTIKLV DITFRNIRGT SENKDAVKLL CSKGHPCENV EIGDINIEYT 370 380 390 GPDGPPTFEC TNVTPKLVGA QNPKACVGPV VKAPGKE

References

[1]

"Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen." Robert L.S., Allard S., Gerster J.L., Cass L., Simmonds J. Plant Mol. Biol. 23:1273-1278(1993) [PubMed: 8292791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Westar. Tissue: Pollen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19879 mRNA. Translation: AAA70402.1.
PIR	S42549.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 393.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_BRANA

Accession

Primary (citable) accession number: P35337

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)