Reviewed, UniProtKB/Swiss-Prot P35336 (PGLR_ACTDE)

Names and origin

Protein names	Recommended name:     Polygalacturonase       Short name=PG     EC=3.2.1.15 Alternative name(s):     Pectinase
Organism	Actinidia deliciosa (Kiwi)
Taxonomic identifier	3627 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Actinidiaceae › Actinidia

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in concert with the pectinesterase, in the ripening process. Is involved in cell wall metabolism, specifically in polyuronide degradation.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Developmental stage	In ripening fruit.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Fruit ripening
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 467	440	Polygalacturonase		PRO_0000024798
Sites
	Active site	283	1	Proton donor By similarity
	Active site	306	1	By similarity
Amino acid modifications
	Glycosylation	290	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P35336-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5A9A61483C028B7A	FASTA	467	50,776
	10 20 30 40 50 60 MALQRRFFQF VIITLLIPSF ILGYTSAVHE DPPHDYHLEE YGYDFKAYPS YITTIGDNDF 70 80 90 100 110 120 GSSMSHENGI FGLRKVDYGM DRVLDASKTV NVDDFGAKGD GRDDTKAFEK AWKAACSSTS 130 140 150 160 170 180 SAVLLVPKKN YLVRPISFSG PCKSGLTMQI YGTIEASDDR SDYRKDGRHW LVFDSVQNLR 190 200 210 220 230 240 VEGGGTINGN GKIWWQNSCK TNKALPCKDA PTALTFYKSK HVIVKNLKIE NAQQIHVSFD 250 260 270 280 290 300 NCVNVQASNL MVTAPENSPN TDGIHVTGTQ NIHISSCVIG TGDDCISIVN GSRKVRVNDI 310 320 330 340 350 360 TCGPGHGISI GSLGYGNSEA HVSDVVVNGA KLCGTTNGVR IKTWQGGSGS ASNIKFQNVE 370 380 390 400 410 420 MHNVENPIII DQNYCDQDKP CQEQSSAVQV KNVVYQNIKG TCASNVAITF DCSKRFPCQG 430 440 450 460 IVLEDVDLEI EGGAAAKALC NNVELSETGV VSPHCQEEGG EEEEEAS

References

[1]	"A polygalacturonase gene from kiwifruit (Actinidia deliciosa)." Atkinson R.G., Gardner R.C. Plant Physiol. 103:669-670(1993) [PubMed: 8029342] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Hayward.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12019 Genomic DNA. Translation: AAC14453.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 67098.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_ACTDE

Accession

Primary (citable) accession number: P35336

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)